BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135464.8 - phase: 0
(559 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CA784687 similar to SP|Q9JX33|DXR_N 1-deoxy-D-xylulose 5-phospha... 141 9e-34
TC220066 similar to UP|Q8DAZ0 (Q8DAZ0) Predicted transcriptional... 102 6e-22
TC224454 83 3e-16
CA938853 similar to GP|1684847|gb|A pinin {Homo sapiens}, partia... 73 3e-13
AW757181 37 0.030
BM178719 37 0.030
TC219288 similar to GB|AAS99672.1|46518381|BT012528 At5g56840 {A... 31 1.3
TC232603 weakly similar to UP|Q39482 (Q39482) Legumin (Fragment)... 31 1.3
TC209804 similar to UP|Q42949 (Q42949) Catechol O-methyltransfer... 30 2.8
TC209805 similar to UP|Q42949 (Q42949) Catechol O-methyltransfer... 30 3.7
TC210186 UP|Q93WQ0 (Q93WQ0) Subtilisin-type protease, complete 30 3.7
BI973638 similar to GP|19550749|gb| caffeic acid O-methyltransfe... 29 6.3
TC219502 similar to UP|Q70DJ9 (Q70DJ9) Calcium binding protein, ... 29 6.3
TC206391 28 8.3
TC228766 similar to GB|AAS99672.1|46518381|BT012528 At5g56840 {A... 28 8.3
TC233008 similar to UP|Q9ASW3 (Q9ASW3) AT3g49260/F2K15_120 (Guar... 28 8.3
>CA784687 similar to SP|Q9JX33|DXR_N 1-deoxy-D-xylulose 5-phosphate
reductoisomerase (EC 1.1.1.267) (DXP reductoisomerase),
partial (6%)
Length = 437
Score = 141 bits (355), Expect = 9e-34
Identities = 63/133 (47%), Positives = 92/133 (68%)
Frame = -1
Query: 5 LGNGYFEFYFSSYEDMRSVWSKGTQNLKPGLLRLFEWSKDFTARTQRQTHAQVWVRLHEL 64
LG G++EF F+S ED+RSVW G NL+P LRL +WS +F RQTHAQ W R+HE
Sbjct: 428 LGKGFYEFSFASLEDLRSVWLVGNWNLQP*SLRLSQWSSNFNPNKVRQTHAQCWKRVHEF 249
Query: 65 PQEYWMDRTLKEIASAIGTPLLIDSATQNRVFGHYARVLVDMDLSKHIFNEVMIERTGFS 124
QEYW + L EI A+G+P+ +D AT+++ FGH++ +L+D+DL+K + EVM+ER ++
Sbjct: 248 SQEYWDLQLLFEIVGAVGSPITLDEATKSKTFGHFSCILMDVDLAKTLHLEVMVERESYA 69
Query: 125 FSIEITYERLPAF 137
F +++ YE F
Sbjct: 68 FLVKVNYENFFPF 30
>TC220066 similar to UP|Q8DAZ0 (Q8DAZ0) Predicted transcriptional regulator,
partial (47%)
Length = 1012
Score = 102 bits (253), Expect = 6e-22
Identities = 52/134 (38%), Positives = 76/134 (55%), Gaps = 1/134 (0%)
Frame = +3
Query: 8 GYFEFYFSSYEDMRSVWSKGT-QNLKPGLLRLFEWSKDFTARTQRQTHAQVWVRLHELPQ 66
G++E F S ED+ SV S GT NL P ++ L W+ DF + T+ W+ L+ L
Sbjct: 411 GFYEIVFFSDEDL*SVRSMGTWNNLNPSVICLLAWT*DFNPGKLKTTNTHCWMWLYGLAY 590
Query: 67 EYWMDRTLKEIASAIGTPLLIDSATQNRVFGHYARVLVDMDLSKHIFNEVMIERTGFSFS 126
EYW + L EI AIG+P+ +D T + FGHYAR+LVD DL+ + ++++ER G S
Sbjct: 591 EYWHPQILVEIGGAIGSPIALDENTVKKTFGHYARILVDFDLTNKLLKQILVERKGLPSS 770
Query: 127 IEITYERLPAFCTH 140
+ +R A H
Sbjct: 771 WGWSMKRFHASALH 812
Score = 29.3 bits (64), Expect = 4.8
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = +2
Query: 122 GFSFSIEITYERLPAFCTHC 141
GF+F + + YE++P FC+ C
Sbjct: 755 GFAFFVGVEYEKVPRFCSTC 814
>TC224454
Length = 660
Score = 83.2 bits (204), Expect = 3e-16
Identities = 40/84 (47%), Positives = 54/84 (63%)
Frame = +2
Query: 5 LGNGYFEFYFSSYEDMRSVWSKGTQNLKPGLLRLFEWSKDFTARTQRQTHAQVWVRLHEL 64
LG G+++F F+S ED+ VW G+ L+ G+LRL +W DF RQTH +WVRL EL
Sbjct: 404 LGCGFYKFNFASLEDLHKVWIAGSWKLQLGILRLTQWIPDFNPYNMRQTHV*LWVRLFEL 583
Query: 65 PQEYWMDRTLKEIASAIGTPLLID 88
P EYW R L EI + IG +++D
Sbjct: 584 PLEYWRPR-LCEITTYIGMSIILD 652
>CA938853 similar to GP|1684847|gb|A pinin {Homo sapiens}, partial (2%)
Length = 422
Score = 73.2 bits (178), Expect = 3e-13
Identities = 39/94 (41%), Positives = 58/94 (61%), Gaps = 7/94 (7%)
Frame = -3
Query: 439 LWSKDLSITV---LFVSDQC----IALQISCHQSIVYIAAVYASTYYLKRRQLWADLTHI 491
L K+LS+ + LF S C +A ++S + AAVYAST Y+KRR LW++LT +
Sbjct: 285 LQKKNLSLKLSPKLFKSFDCSHQ*VAFKLSLGSKTSFFAAVYASTAYVKRRDLWSELTDL 106
Query: 492 QEHFVGPWLFVGDFNAVLGAHEKCGRRPPPPLSC 525
Q+ +G W F+GDFN+ +GA EK G + ++C
Sbjct: 105 QQKHLGSWPFIGDFNSNVGAQEKRGGKMSCSIAC 4
>AW757181
Length = 422
Score = 36.6 bits (83), Expect = 0.030
Identities = 32/139 (23%), Positives = 52/139 (37%)
Frame = +3
Query: 8 GYFEFYFSSYEDMRSVWSKGTQNLKPGLLRLFEWSKDFTARTQRQTHAQVWVRLHELPQE 67
GY+ F+S E ++ G+ + + + W F ++ WV+ LP +
Sbjct: 9 GYYLVQFTSEEGYKNAMFNGS*MVADHYIIV*RWKPTFIQNAKKM*KVAEWVQFLLLPMK 188
Query: 68 YWMDRTLKEIASAIGTPLLIDSATQNRVFGHYARVLVDMDLSKHIFNEVMIERTGFSFSI 127
+ D L S +GT L ID T ++ V++ L+K R F
Sbjct: 189 LYHDVFL*TTWSLLGTILNID*CTSIHSKARFSPTCVEVVLNKTKLVSKFEARWNVYF-- 362
Query: 128 EITYERLPAFCTHCGNIGH 146
+ L C CG GH
Sbjct: 363 -VE*NGLHLICFQCGTYGH 416
>BM178719
Length = 378
Score = 36.6 bits (83), Expect = 0.030
Identities = 16/33 (48%), Positives = 22/33 (66%)
Frame = +2
Query: 493 EHFVGPWLFVGDFNAVLGAHEKCGRRPPPPLSC 525
+ + GP F+GD NA+LG HEK G + P +SC
Sbjct: 92 QRYPGP*CFLGDNNAILGEHEKRGGKLPLYISC 190
>TC219288 similar to GB|AAS99672.1|46518381|BT012528 At5g56840 {Arabidopsis
thaliana;} , partial (41%)
Length = 720
Score = 31.2 bits (69), Expect = 1.3
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = +2
Query: 133 RLPAFCTHCGNIGHHNSSC 151
R+ C+HCGNIGH++ +C
Sbjct: 20 RMGRKCSHCGNIGHNSRTC 76
>TC232603 weakly similar to UP|Q39482 (Q39482) Legumin (Fragment), partial
(4%)
Length = 1123
Score = 31.2 bits (69), Expect = 1.3
Identities = 15/42 (35%), Positives = 21/42 (49%), Gaps = 1/42 (2%)
Frame = +1
Query: 481 RRQLWADLTHIQEHFVGP-WLFVGDFNAVLGAHEKCGRRPPP 521
++Q W ++ I+ G W VGDFNAV E+ G P
Sbjct: 373 KKQEWEEIIRIKNEMGGERWCIVGDFNAVCSEEERKGVETNP 498
>TC209804 similar to UP|Q42949 (Q42949) Catechol O-methyltransferase ,
partial (50%)
Length = 676
Score = 30.0 bits (66), Expect = 2.8
Identities = 18/62 (29%), Positives = 29/62 (46%)
Frame = -2
Query: 189 GSSKAFEAPVGNNNAESIPTTHKVATKLPVQTDLTESADAQHSDSANVSAAIHHSDDEDN 248
G + E P G ++ T+ +LP Q + +HS S+ S +IHHS+ E +
Sbjct: 471 GFESSSEHPFGKRPSKQHLTSLV*LEELPPQLSPFHHQELRHSSSSTTSDSIHHSNHEVS 292
Query: 249 LS 250
S
Sbjct: 291 TS 286
>TC209805 similar to UP|Q42949 (Q42949) Catechol O-methyltransferase ,
partial (24%)
Length = 946
Score = 29.6 bits (65), Expect = 3.7
Identities = 17/58 (29%), Positives = 27/58 (46%)
Frame = -2
Query: 189 GSSKAFEAPVGNNNAESIPTTHKVATKLPVQTDLTESADAQHSDSANVSAAIHHSDDE 246
G + E P G ++ T+ +LP Q + +HS S+ S +IHHS+ E
Sbjct: 672 GFESSSEHPFGKRPSKQHLTSLV*LEELPPQLSPFHHQELRHSSSSTTSDSIHHSNHE 499
>TC210186 UP|Q93WQ0 (Q93WQ0) Subtilisin-type protease, complete
Length = 2401
Score = 29.6 bits (65), Expect = 3.7
Identities = 13/32 (40%), Positives = 16/32 (49%), Gaps = 5/32 (15%)
Frame = -2
Query: 135 PAFCTHCGN-----IGHHNSSCRWLHPVKEPL 161
P CT C N + HHN+SCR H P+
Sbjct: 786 PTLCTPCRNTVVRCVSHHNTSCRSRHMGSMPI 691
>BI973638 similar to GP|19550749|gb| caffeic acid O-methyltransferase II
{Nicotiana tabacum}, partial (14%)
Length = 422
Score = 28.9 bits (63), Expect = 6.3
Identities = 15/40 (37%), Positives = 22/40 (54%), Gaps = 5/40 (12%)
Frame = -3
Query: 227 DAQHSDSANVSAAIHHSDDEDNLSA-----AAHHSDDVSP 261
+ QHS S+ S+ IHHS+ E + S+ AH +SP
Sbjct: 348 ELQHSSSSTPSSTIHHSNHEAHTSSKWHLLGAHFQTYISP 229
>TC219502 similar to UP|Q70DJ9 (Q70DJ9) Calcium binding protein, partial
(74%)
Length = 883
Score = 28.9 bits (63), Expect = 6.3
Identities = 14/60 (23%), Positives = 33/60 (54%)
Frame = +3
Query: 390 KASLRKGFVQHPRKGYSSSLILMNILFWNIRVTKYFLNVRDNLQPNLWALWSKDLSITVL 449
+ASL+K +H K I MN + W++R+ ++ + +++ +++ W +LS ++
Sbjct: 666 EASLKKMEKEHSDKKAK*IKIDMNYISWDMRLCEFLVECLESVIYGVYSYWCMNLSYVLI 845
>TC206391
Length = 755
Score = 28.5 bits (62), Expect = 8.3
Identities = 13/34 (38%), Positives = 22/34 (64%)
Frame = -1
Query: 263 NVTKDPLDFEFIPVMPDDINKATLPSTSIHVLEE 296
N T+D +++ F V PD N ++LPST I ++ +
Sbjct: 491 NFTQDKIEYHFCAV-PDRTNPSSLPSTKITLINK 393
>TC228766 similar to GB|AAS99672.1|46518381|BT012528 At5g56840 {Arabidopsis
thaliana;} , partial (54%)
Length = 1220
Score = 28.5 bits (62), Expect = 8.3
Identities = 8/14 (57%), Positives = 12/14 (85%)
Frame = +3
Query: 138 CTHCGNIGHHNSSC 151
C+HCG IGH++ +C
Sbjct: 138 CSHCGTIGHNSRTC 179
>TC233008 similar to UP|Q9ASW3 (Q9ASW3) AT3g49260/F2K15_120 (Guard cell
associated protein) (SF16-like protein), partial (29%)
Length = 700
Score = 28.5 bits (62), Expect = 8.3
Identities = 22/71 (30%), Positives = 33/71 (45%), Gaps = 2/71 (2%)
Frame = +1
Query: 262 QNVTKDPLDFEFIPV--MPDDINKATLPSTSIHVLEETADTAEVPIEDTTAAAEPLFTDV 319
Q+ + + FE P PD+++ A + +TS V E A T AAAE T V
Sbjct: 220 QHEVAEVVSFEHFPAESSPDNVSNAEMSTTSTPVTNEDRSHAIAVAAATAAAAE---TAV 390
Query: 320 MALEAQTQTNR 330
+A +A + R
Sbjct: 391 VAAQAAARVVR 423
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.134 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,250,877
Number of Sequences: 63676
Number of extensions: 438608
Number of successful extensions: 2378
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 2355
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2376
length of query: 559
length of database: 12,639,632
effective HSP length: 102
effective length of query: 457
effective length of database: 6,144,680
effective search space: 2808118760
effective search space used: 2808118760
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC135464.8