BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135464.2 - phase: 0 /pseudo
(1353 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC231899 similar to UP|Q850H7 (Q850H7) Gag-pol polyprotein (Frag... 138 1e-32
BQ296988 similar to GP|21740616|em OSJNBb0089K24.12 {Oryza sativ... 131 2e-30
TC231544 weakly similar to UP|Q9FLR2 (Q9FLR2) Polyprotein-like, ... 124 3e-28
TC225402 weakly similar to UP|Q6I923 (Q6I923) Copia-like retrotr... 114 3e-25
BU764568 99 2e-23
TC204438 homologue to UP|Q84VH6 (Q84VH6) Gag-pol polyprotein, co... 103 5e-22
TC204439 UP|Q84VI4 (Q84VI4) Gag-pol polyprotein, complete 103 5e-22
BU548243 92 2e-18
BG508993 92 2e-18
BG882494 weakly similar to GP|27901694|gb| gag-pol polyprotein {... 90 6e-18
TC223792 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polypro... 84 4e-16
BE474381 similar to GP|21434|emb|CA ORF4 {Solanum tuberosum}, pa... 79 1e-14
TC213888 similar to UP|Q9SFE1 (Q9SFE1) T26F17.17, partial (11%) 78 2e-14
BU549979 78 3e-14
BU550032 similar to GP|27901696|gb| gag-pol polyprotein {Vitis v... 75 1e-13
CA935263 52 3e-13
TC213445 65 5e-13
TC211663 68 3e-11
AW185460 66 9e-11
BI969608 similar to GP|21434|emb|CA ORF4 {Solanum tuberosum}, pa... 64 6e-10
>TC231899 similar to UP|Q850H7 (Q850H7) Gag-pol polyprotein (Fragment), partial
(30%)
Length = 687
Score = 138 bits (348), Expect = 1e-32
Identities = 65/107 (60%), Positives = 79/107 (73%)
Frame = +2
Query: 1195 LLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCEL 1254
L GY DADW GCP RRS +GYC FIG +LVSWKSKKQ +V+RSS+EAEYR++ TCEL
Sbjct: 17 LSGYCDADWAGCPMDRRSTSGYCVFIGGNLVSWKSKKQTVVARSSAEAEYRSMAMVTCEL 196
Query: 1255 Q*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLDIDC 1301
+ L +L L CD+Q+ALHIASNP+FHERTKH++IDC
Sbjct: 197 MWIKQFLQELRFCEELQMKLYCDNQAALHIASNPVFHERTKHIEIDC 337
>BQ296988 similar to GP|21740616|em OSJNBb0089K24.12 {Oryza sativa (japonica
cultivar-group)}, partial (1%)
Length = 408
Score = 131 bits (329), Expect = 2e-30
Identities = 55/113 (48%), Positives = 85/113 (74%)
Frame = -1
Query: 2 RCNNLVHSWLMNSVTPSITQSLIYTDSAVDAWNDLQARFSRADRVRISTLQRDLHSFRQN 61
RCN L+HSW++NSV PSI++S+++ D+A D W DL+ RFS+ D VR+S +Q+++++ Q
Sbjct: 408 RCNMLIHSWILNSVEPSISRSIVFMDNASDVWLDLKERFSQGDLVRVSEIQQEIYALTQG 229
Query: 62 SLSIIEYFTELKSYWEELEVFCPIPNCTCNVRCSCEAMRNSRRGSDTPLSHRF 114
+ S+ ++++ K+ WEELE++ PIPNCTC+ RCSC+AMR +RR T RF
Sbjct: 228 TRSVTTFYSDKKALWEELEIYMPIPNCTCHHRCSCDAMRLARRHHHTLHVMRF 70
>TC231544 weakly similar to UP|Q9FLR2 (Q9FLR2) Polyprotein-like, partial (16%)
Length = 662
Score = 124 bits (311), Expect = 3e-28
Identities = 69/133 (51%), Positives = 90/133 (66%), Gaps = 3/133 (2%)
Frame = +3
Query: 1181 PGTGLFFPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSR--S 1238
P GL F R+S + +LG+SDADW C D+ +SIT YCFF+G SL+SWK+KKQ VSR S
Sbjct: 54 PRKGLSFSRESPIQILGFSDADWATCIDSSKSITWYCFFLGSSLISWKAKKQNTVSRSSS 233
Query: 1239 SSEAEYRALVASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALH-IASNPIFHERTKHL 1297
SSEA+YRAL ++TCELQ L YLL DL+VT + CD+QSAL + I+H + L
Sbjct: 234 SSEAKYRALTSTTCELQWLTYLLKDLHVTL-----IYCDNQSALQ*LPIKVIYHGQ---L 389
Query: 1298 DIDCQCSEGETSK 1310
+IDC +T +
Sbjct: 390 EIDCHIVREKTQQ 428
>TC225402 weakly similar to UP|Q6I923 (Q6I923) Copia-like retrotransposon
Hopscotch polyprotein, partial (7%)
Length = 1446
Score = 114 bits (285), Expect = 3e-25
Identities = 55/102 (53%), Positives = 72/102 (69%)
Frame = +2
Query: 1200 DADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCELQ*LVY 1259
DA+W P R S GYC IG +LV WKS K +V+RSS+EAEY+A+ +TCEL +
Sbjct: 8 DANWAVSPIDRGSTLGYCVSIGENLVLWKSNK*NVVARSSAEAEYKAMTVATCELIWIKQ 187
Query: 1260 LLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLDIDC 1301
LL +L ++ L CD+Q+ALHIASNP+FHERTKH++IDC
Sbjct: 188 LLQELKFGSTQQMKLCCDNQAALHIASNPVFHERTKHIEIDC 313
>BU764568
Length = 420
Score = 99.0 bits (245), Expect(2) = 2e-23
Identities = 49/84 (58%), Positives = 62/84 (73%)
Frame = +3
Query: 1214 TGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCELQ*LVYLLTDLNVTCSKVPG 1273
+GYC IG +L+SWKSKKQ +V++SS+EAEYRA+ TCEL L LL +L
Sbjct: 168 SGYCVLIGGNLISWKSKKQSVVAKSSAEAEYRAMALVTCELIWLKQLL*ELKFEEDTQMT 347
Query: 1274 LLCDSQSALHIASNPIFHERTKHL 1297
L+CD+Q+ALHIASNPIFH RTKH+
Sbjct: 348 LICDNQAALHIASNPIFH*RTKHI 419
Score = 30.4 bits (67), Expect(2) = 2e-23
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +1
Query: 1178 ETSPGTGLFFPRDSSLHLLGYSDADWGGCPDTR 1210
+++PG GL + ++GYSDAD G P R
Sbjct: 67 KSAPGKGLIYEDKGHSQIIGYSDAD*VGSPSDR 165
>TC204438 homologue to UP|Q84VH6 (Q84VH6) Gag-pol polyprotein, complete
Length = 4734
Score = 103 bits (257), Expect = 5e-22
Identities = 53/116 (45%), Positives = 75/116 (63%)
Frame = +1
Query: 1184 GLFFPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAE 1243
G+ + S L+GY DADW G D R+S +G CF++G +L+SW SKKQ VS S++EAE
Sbjct: 4228 GIMYCHCSDSMLVGYCDADWAGSADDRKSTSGGCFYLGTNLISWFSKKQNCVSLSTAEAE 4407
Query: 1244 YRALVASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLDI 1299
Y A +S +L + +L + NV V L CD+ SA++I+ NP+ H RTKH+DI
Sbjct: 4408 YIAAGSSCSQLVWMKQMLKEYNVE-QDVMTLYCDNMSAINISKNPVQHSRTKHIDI 4572
>TC204439 UP|Q84VI4 (Q84VI4) Gag-pol polyprotein, complete
Length = 4731
Score = 103 bits (257), Expect = 5e-22
Identities = 53/116 (45%), Positives = 76/116 (64%)
Frame = +1
Query: 1184 GLFFPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAE 1243
G+ + S+ L+GY DADW G D R+S +G CF++G +L+SW SKKQ VS S++EAE
Sbjct: 4225 GIMYCHCSNPMLVGYCDADWAGSADDRKSTSGGCFYLGNNLISWFSKKQNCVSLSTAEAE 4404
Query: 1244 YRALVASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLDI 1299
Y A +S +L + +L + NV V L CD+ SA++I+ NP+ H RTKH+DI
Sbjct: 4405 YIAAGSSCSQLVWMKQMLKEYNVE-QDVMTLYCDNMSAINISKNPVQHSRTKHIDI 4569
>BU548243
Length = 599
Score = 92.0 bits (227), Expect = 2e-18
Identities = 45/101 (44%), Positives = 68/101 (66%)
Frame = -1
Query: 1200 DADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCELQ*LVY 1259
DA W D RS G F+G +L+SW S+KQQ+ ++SS+EAEYR++ ++ EL +
Sbjct: 587 DAGWASDVDDHRSTLGSAIFLGPNLISWWSRKQQVTAQSSTEAEYRSIAQTSAELTWIQA 408
Query: 1260 LLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLDID 1300
LL +L + + P +LCD++SA+ IA N +FH RTKH++ID
Sbjct: 407 LLMELQIPFTP-PVILCDNKSAVAIAHNLVFHSRTKHMEID 288
>BG508993
Length = 374
Score = 91.7 bits (226), Expect = 2e-18
Identities = 46/115 (40%), Positives = 71/115 (61%)
Frame = +1
Query: 1184 GLFFPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAE 1243
GLF+ ++ L+G+ D+D+ G D R+S TG+ FF+G + +W SKKQ IV+ + EAE
Sbjct: 22 GLFYSPSNNYKLVGFCDSDFAGDVDDRKSTTGFVFFMGDCVFTWSSKKQGIVTLFTCEAE 201
Query: 1244 YRALVASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLD 1298
Y A + TC L LL +L + + + D++SA +A N +FHER+KH+D
Sbjct: 202 YVAATSCTCHAIWLRRLLEELQLLQKESTKIYVDNRSAQELAKNSVFHERSKHID 366
>BG882494 weakly similar to GP|27901694|gb| gag-pol polyprotein {Vitis
vinifera}, partial (20%)
Length = 400
Score = 90.1 bits (222), Expect = 6e-18
Identities = 49/123 (39%), Positives = 70/123 (56%)
Frame = -2
Query: 1179 TSPGTGLFFPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRS 1238
++PG GL + ++GY D DW G +R YC I L+SW SKK +V+RS
Sbjct: 381 SAPGKGLTYEDREHTQIVGYLDVDWAGSAIHKRPTCRYCVLIRGKLISWNSKKHNVVTRS 202
Query: 1239 SSEAEYRALVASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKHLD 1298
++ EYRA+ A C+L L L + L+CD+Q+AL+IASN F RTKH++
Sbjct: 201 RTKVEYRAM-ALICQLIWFKQLRKQLQFEDTMQMTLICDNQAALYIASNQ-FSTRTKHIE 28
Query: 1299 IDC 1301
I+C
Sbjct: 27 INC 19
>TC223792 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein, partial
(7%)
Length = 804
Score = 84.0 bits (206), Expect = 4e-16
Identities = 46/138 (33%), Positives = 77/138 (55%), Gaps = 3/138 (2%)
Frame = +1
Query: 1176 LFETSPGTGLFFP---RDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQ 1232
L + + G+G+ FP + LLGY+D+DW P+ +S GY F + V+ SKKQ
Sbjct: 157 LIKGTIGSGVLFPFKAKSGKPDLLGYTDSDWKRDPEQEKSTGGYLFMYNDAPVA*SSKKQ 336
Query: 1233 QIVSRSSSEAEYRALVASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHE 1292
+++ S+ EAEY A C+ ++ LL +L + K LL D++SA+++A +P H
Sbjct: 337 DVIALSTCEAEYVAASLGACQAVWMMNLLEELKLRERKPVNLLIDNKSAINLAKHPTLHG 516
Query: 1293 RTKHLDIDCQCSEGETSK 1310
R+KH+++ + SK
Sbjct: 517 RSKHIELRFHYIRDQVSK 570
>BE474381 similar to GP|21434|emb|CA ORF4 {Solanum tuberosum}, partial (20%)
Length = 406
Score = 79.3 bits (194), Expect = 1e-14
Identities = 44/85 (51%), Positives = 56/85 (65%)
Frame = +3
Query: 1197 GYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCELQ* 1256
GY+DADW G RRS +GYC F+G +LVS SKKQ +V+RSS+EAE+RAL CE
Sbjct: 153 GYTDADWAGSVTDRRSTSGYCTFVGGNLVS*-SKKQSVVARSSAEAEFRALAHGICETLW 329
Query: 1257 LVYLLTDLNVTCSKVPGLLCDSQSA 1281
+ LL +L V S L CD++SA
Sbjct: 330 VKKLLQELKVHSSPPIKLYCDNKSA 404
>TC213888 similar to UP|Q9SFE1 (Q9SFE1) T26F17.17, partial (11%)
Length = 493
Score = 78.2 bits (191), Expect = 2e-14
Identities = 39/91 (42%), Positives = 58/91 (62%)
Frame = +3
Query: 1208 DTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCELQ*LVYLLTDLNVT 1267
D R+S TG+ FF+G + +W SKKQ IV+ S+ EAEY A + C L LL +L +
Sbjct: 9 DDRKSTTGFVFFMGDTAFTWMSKKQPIVTLSTCEAEYVAATSCVCHAIWLRNLLKELKMP 188
Query: 1268 CSKVPGLLCDSQSALHIASNPIFHERTKHLD 1298
+ + D++SAL +A NP+FHE++KH+D
Sbjct: 189 QEEPMEICVDNKSALALAKNPVFHEKSKHID 281
>BU549979
Length = 615
Score = 77.8 bits (190), Expect = 3e-14
Identities = 41/117 (35%), Positives = 69/117 (58%), Gaps = 2/117 (1%)
Frame = -1
Query: 1185 LFFPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEY 1244
L + + + L ++GYSD+D+ GC D+RRS +GY F + +VSW+S KQ +++ S+ E E+
Sbjct: 522 LMYKQTNCLEVIGYSDSDFAGCVDSRRSTSGYIFMLADGVVSWRSSKQTLIATSTMEVEF 343
Query: 1245 RALVASTCELQ*LVYLLTDLNV--TCSKVPGLLCDSQSALHIASNPIFHERTKHLDI 1299
+T L ++ L V + S+ L CD+ +A+ +A N R+KH+DI
Sbjct: 342 VPCFEATSHGVWLKSFMSSLRVVDSISRPLKLYCDNFAAVFMAKNNKSGNRSKHIDI 172
>BU550032 similar to GP|27901696|gb| gag-pol polyprotein {Vitis vinifera},
partial (21%)
Length = 698
Score = 75.5 bits (184), Expect = 1e-13
Identities = 37/74 (50%), Positives = 49/74 (66%)
Frame = -2
Query: 1228 KSKKQQIVSRSSSEAEYRALVASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHIASN 1287
KSKKQ +V+ S+E EY ++ TCEL + L +L L CD+Q+ALHIASN
Sbjct: 697 KSKKQTVVA*XSAEVEY*SMTMVTCELMWIKQXLQELRFCEVVQMKLYCDNQAALHIASN 518
Query: 1288 PIFHERTKHLDIDC 1301
P+FHER KH++IDC
Sbjct: 517 PVFHERAKHIEIDC 476
>CA935263
Length = 446
Score = 51.6 bits (122), Expect(2) = 3e-13
Identities = 22/40 (55%), Positives = 28/40 (70%)
Frame = +3
Query: 1203 WGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEA 1242
W G P RS +GYC IG +L+SW+SKKQ +RSS+EA
Sbjct: 3 WEGSPSNSRSTSGYCVLIGENLISWRSKKQNTFARSSAEA 122
Score = 43.1 bits (100), Expect(2) = 3e-13
Identities = 21/53 (39%), Positives = 31/53 (57%)
Frame = +1
Query: 1244 YRALVASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNPIFHERTKH 1296
Y A+ A+ E+ LL L ++ L+C +Q+ LHIAS P+FHE+T H
Sbjct: 124 YCAMTAAAIEITWPRQLLQQLRFGDTQATKLICHNQATLHIASTPVFHEQTTH 282
>TC213445
Length = 705
Score = 64.7 bits (156), Expect(2) = 5e-13
Identities = 35/94 (37%), Positives = 55/94 (58%)
Frame = +1
Query: 1206 CPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAEYRALVASTCELQ*LVYLLTDLN 1265
C R S + C FIG +LVSW SKKQ V S++EAEY + + ++ + L D
Sbjct: 397 CKTDRESTSDTCHFIGSALVSWHSKKQNSVVLSTAEAEYISARSYYAQIFWMRQQLFDYG 576
Query: 1266 VTCSKVPGLLCDSQSALHIASNPIFHERTKHLDI 1299
+ +P + CD+ SA++++ N I + RTKH++I
Sbjct: 577 LKLDHIP-IRCDNTSAINLSKNHILYSRTKHIEI 675
Score = 29.3 bits (64), Expect(2) = 5e-13
Identities = 11/18 (61%), Positives = 17/18 (94%)
Frame = +2
Query: 1184 GLFFPRDSSLHLLGYSDA 1201
GL++P++SS +L+GYSDA
Sbjct: 347 GLWYPKNSSYNLVGYSDA 400
>TC211663
Length = 426
Score = 67.8 bits (164), Expect = 3e-11
Identities = 30/75 (40%), Positives = 50/75 (66%)
Frame = -3
Query: 19 ITQSLIYTDSAVDAWNDLQARFSRADRVRISTLQRDLHSFRQNSLSIIEYFTELKSYWEE 78
I Q +IY D A + WNDL+ FS+ D ++I+ LQ +++ +Q S +++++FT+LK WEE
Sbjct: 424 IAQIVIYFDHATNIWNDLKEGFSQGDLLQIAELQEEIYRLKQGSHTVLDFFTKLKFVWEE 245
Query: 79 LEVFCPIPNCTCNVR 93
L+ + + CTC R
Sbjct: 244 LDNYGLMNLCTCPSR 200
>AW185460
Length = 411
Score = 66.2 bits (160), Expect = 9e-11
Identities = 28/64 (43%), Positives = 45/64 (69%)
Frame = +2
Query: 1184 GLFFPRDSSLHLLGYSDADWGGCPDTRRSITGYCFFIG*SLVSWKSKKQQIVSRSSSEAE 1243
G+++ +++ LLGY+D+DW G D +S +GY F +G + SW SKKQ V++S++EAE
Sbjct: 212 GIWYTTETNSELLGYTDSDWAGSTDDMKSTSGYAFSLGSGMFSWASKKQATVAQSTAEAE 391
Query: 1244 YRAL 1247
Y A+
Sbjct: 392 YVAV 403
>BI969608 similar to GP|21434|emb|CA ORF4 {Solanum tuberosum}, partial (19%)
Length = 454
Score = 63.5 bits (153), Expect = 6e-10
Identities = 29/72 (40%), Positives = 49/72 (67%)
Frame = -2
Query: 1229 SKKQQIVSRSSSEAEYRALVASTCELQ*LVYLLTDLNVTCSKVPGLLCDSQSALHIASNP 1288
+K + +RSS++AEYRA+ CE+ *L +L +L + + L CD+++A++I+ NP
Sbjct: 426 TKNKMXXARSSAKAEYRAMAQGVCEIL*LKRILEELQLPMTLPMKLYCDNKAAINISQNP 247
Query: 1289 IFHERTKHLDID 1300
+ H RTKH++ID
Sbjct: 246 VQHGRTKHVEID 211
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.360 0.159 0.618
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,572,806
Number of Sequences: 63676
Number of extensions: 1421646
Number of successful extensions: 19104
Number of sequences better than 10.0: 134
Number of HSP's better than 10.0 without gapping: 10407
Number of HSP's successfully gapped in prelim test: 880
Number of HSP's that attempted gapping in prelim test: 7592
Number of HSP's gapped (non-prelim): 12844
length of query: 1353
length of database: 12,639,632
effective HSP length: 109
effective length of query: 1244
effective length of database: 5,698,948
effective search space: 7089491312
effective search space used: 7089491312
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.9 bits)
S2: 65 (29.6 bits)
Medicago: description of AC135464.2