BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135461.10 + phase: 0
(425 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC219506 similar to UP|Q9M8Z1 (Q9M8Z1) T6K12.3 protein (AT3g0435... 404 e-113
TC210800 similar to UP|Q9M8Z1 (Q9M8Z1) T6K12.3 protein (AT3g0435... 347 8e-96
BE609440 weakly similar to GP|20197184|gb expressed protein {Ara... 187 8e-48
CO984562 120 1e-27
BF595188 similar to GP|15450691|gb| AT3g04350/T6K12_3 {Arabidops... 112 3e-25
TC210620 31 1.2
BU765852 29 4.6
TC225904 similar to UP|O48937 (O48937) NADPH cytochrome P450 red... 29 4.6
BI967853 28 6.0
TC228141 similar to GB|AAL77730.1|18700240|AY078029 AT3g54810/F2... 28 6.0
TC232084 UP|Q84PP2 (Q84PP2) Transcription factor MYB101 (Fragmen... 28 7.8
>TC219506 similar to UP|Q9M8Z1 (Q9M8Z1) T6K12.3 protein (AT3g04350/T6K12_3),
partial (36%)
Length = 995
Score = 404 bits (1037), Expect = e-113
Identities = 186/212 (87%), Positives = 201/212 (94%)
Frame = +3
Query: 214 LGGAFTDIAMWVFCPFNGPATLKVSLMNIEMNKIGEHVGDWEHFTLRVSNFTGELWSVFF 273
LGGAFTDI MWVFCPFNGPATLKV+LMNIEM+KIGEHVGDWEHFTLR+SNFTGELWSV+F
Sbjct: 3 LGGAFTDIVMWVFCPFNGPATLKVALMNIEMSKIGEHVGDWEHFTLRISNFTGELWSVYF 182
Query: 274 SEHSGGKWVNAFDLEFIKENKPIVYSSRHGHASYPHAGTYLQGSSKLGIGVRNDAAKSNF 333
S+HSGG W++AFDLEF K NKPIVYSS+ GHAS+PH GTYLQGSSKLGIGVRNDAA S F
Sbjct: 183 SQHSGGGWIHAFDLEFNKGNKPIVYSSKDGHASFPHPGTYLQGSSKLGIGVRNDAAPSKF 362
Query: 334 ILDSSFRYKIVAAEYLGDGVITEPCWLQYMREWGPTIVYDSRSEIEKIIDMLPIFVRFSV 393
I+DSS +Y+IVAAEYLGDGVI EPCWLQYMREWGPTIVYDSRSEIEKII++LP+FVRFSV
Sbjct: 363 IVDSSIKYQIVAAEYLGDGVIAEPCWLQYMREWGPTIVYDSRSEIEKIINLLPLFVRFSV 542
Query: 394 ENLFELFPTELSGEEGPTGPKEKDNWLGDEYC 425
ENLFELFPTEL GEEGPTGPKEKDNWLGDEYC
Sbjct: 543 ENLFELFPTELYGEEGPTGPKEKDNWLGDEYC 638
>TC210800 similar to UP|Q9M8Z1 (Q9M8Z1) T6K12.3 protein (AT3g04350/T6K12_3),
partial (35%)
Length = 986
Score = 347 bits (889), Expect = 8e-96
Identities = 155/228 (67%), Positives = 197/228 (85%)
Frame = +1
Query: 196 LKKGNIESAELYVHVKPALGGAFTDIAMWVFCPFNGPATLKVSLMNIEMNKIGEHVGDWE 255
++ G+++SA+LYVHVKPALGG FTDIAMWVFCPFNGP+TLK+ + + +K+GEHVGDWE
Sbjct: 10 IQAGDLKSAKLYVHVKPALGGTFTDIAMWVFCPFNGPSTLKIGITSRAFSKVGEHVGDWE 189
Query: 256 HFTLRVSNFTGELWSVFFSEHSGGKWVNAFDLEFIKENKPIVYSSRHGHASYPHAGTYLQ 315
HFTLR+ NF+GELWS++FS+HSGGKWV+A++LE+I NK +VYSS++GHASYPH GTYLQ
Sbjct: 190 HFTLRICNFSGELWSIYFSQHSGGKWVDAYELEYIDGNKAVVYSSKNGHASYPHPGTYLQ 369
Query: 316 GSSKLGIGVRNDAAKSNFILDSSFRYKIVAAEYLGDGVITEPCWLQYMREWGPTIVYDSR 375
GSSKLGIG+RNDA +SN +DSS +Y++VAAEYLGD V+ EP WLQ+MREWGP IVYDS+
Sbjct: 370 GSSKLGIGIRNDATRSNLYVDSSVQYELVAAEYLGD-VVREPQWLQFMREWGPKIVYDSK 546
Query: 376 SEIEKIIDMLPIFVRFSVENLFELFPTELSGEEGPTGPKEKDNWLGDE 423
+E++KII+ LP +R S +L + P EL GEEGPTGPKEK+NW+GDE
Sbjct: 547 TELDKIINALPGGLRNSFVSLIKKLPVELYGEEGPTGPKEKNNWIGDE 690
>BE609440 weakly similar to GP|20197184|gb expressed protein {Arabidopsis
thaliana}, partial (26%)
Length = 461
Score = 187 bits (475), Expect = 8e-48
Identities = 88/152 (57%), Positives = 107/152 (69%), Gaps = 1/152 (0%)
Frame = +1
Query: 146 WFFKNGAILYTAGN-AKGKAIDYHGTNLPGGGYNDGAFWIDLPTDEDARSNLKKGNIESA 204
WFF NGA+LY G +K +I +G NLP DGA+W+DLP D + +KKG+++SA
Sbjct: 1 WFFSNGALLYKKGQESKPVSISPNGANLPQDPNIDGAYWVDLPADSTNKERVKKGDLKSA 180
Query: 205 ELYVHVKPALGGAFTDIAMWVFCPFNGPATLKVSLMNIEMNKIGEHVGDWEHFTLRVSNF 264
YVHVKP LGG FTDIAMWVF PFNGPA KV + + + KIGEHVGDWEH TLRVSNF
Sbjct: 181 ISYVHVKPMLGGTFTDIAMWVFYPFNGPARAKVEFLTVNLGKIGEHVGDWEHVTLRVSNF 360
Query: 265 TGELWSVFFSEHSGGKWVNAFDLEFIKENKPI 296
GEL V+FS+HS G W ++ LEF NKP+
Sbjct: 361 NGELKHVYFSQHSKGVWFDSSQLEFQSGNKPL 456
>CO984562
Length = 623
Score = 120 bits (301), Expect = 1e-27
Identities = 65/156 (41%), Positives = 84/156 (53%)
Frame = -1
Query: 268 LWSVFFSEHSGGKWVNAFDLEFIKENKPIVYSSRHGHASYPHAGTYLQGSSKLGIGVRND 327
LW V+FS+HS G+W +A +LEF N+P+ YSS HGHA +P G +QG G+GVRND
Sbjct: 623 LWKVYFSQHSKGQWEDASELEFQNGNRPVAYSSLHGHALFPKPGLVMQGMR--GLGVRND 450
Query: 328 AAKSNFILDSSFRYKIVAAEYLGDGVITEPCWLQYMREWGPTIVYDSRSEIEKIIDMLPI 387
AAKS+ ++D + ++IVAAEYLG I EP WL + WGP
Sbjct: 449 AAKSDAVMDMATWFEIVAAEYLG-SQIREPPWLNFCMNWGP------------------- 330
Query: 388 FVRFSVENLFELFPTELSGEEGPTGPKEKDNWLGDE 423
+EGP GPK+KD W GDE
Sbjct: 329 -------------------KEGPKGPKQKDMWKGDE 279
>BF595188 similar to GP|15450691|gb| AT3g04350/T6K12_3 {Arabidopsis
thaliana}, partial (10%)
Length = 249
Score = 112 bits (280), Expect = 3e-25
Identities = 51/82 (62%), Positives = 65/82 (79%)
Frame = +2
Query: 82 VSVGTFFCGTYFDSEQVVDVVCLKNLDSLLHAMPNLNQIHALIEHYGPTVYFHPDEKYMP 141
VSVGTFFC + +++ + VVCLKNL+ +L AMP L+ IHALIEHYGPTV+FHP+E Y+P
Sbjct: 2 VSVGTFFCTSCWNNGDELSVVCLKNLNPVLPAMPRLH*IHALIEHYGPTVFFHPEEVYLP 181
Query: 142 SSVSWFFKNGAILYTAGNAKGK 163
SSV WFF NGA+LY G + G+
Sbjct: 182 SSVDWFFNNGALLYRKGVSTGE 247
>TC210620
Length = 698
Score = 30.8 bits (68), Expect = 1.2
Identities = 15/44 (34%), Positives = 20/44 (45%)
Frame = +1
Query: 11 WLPNPPKGYKAVGIVVTTNPDEPKAEEVRCVRTDLTEVCETSDL 54
W P P G+ A G V EP+ + V C+ L E E +L
Sbjct: 91 WHPRAPDGFVAPGCVAIAGYLEPEPDLVYCIAESLVEETEFEEL 222
>BU765852
Length = 427
Score = 28.9 bits (63), Expect = 4.6
Identities = 12/40 (30%), Positives = 21/40 (52%)
Frame = +2
Query: 300 SRHGHASYPHAGTYLQGSSKLGIGVRNDAAKSNFILDSSF 339
+ H H + H L KLG+ +RN AA+ + +L + +
Sbjct: 164 THHHHLEFSHYNNPLHSVDKLGMRMRNSAARVSLLLGNFY 283
>TC225904 similar to UP|O48937 (O48937) NADPH cytochrome P450 reductase ,
partial (68%)
Length = 1704
Score = 28.9 bits (63), Expect = 4.6
Identities = 16/53 (30%), Positives = 24/53 (45%), Gaps = 1/53 (1%)
Frame = +1
Query: 117 LNQIHALIEHYGPTVYFHPDEKYMPSSVSW-FFKNGAILYTAGNAKGKAIDYH 168
L+++ GPT + + +S W GA +Y G+AKG A D H
Sbjct: 1156 LDELILAFSREGPTKEYVQHKMMEKASEIWSMISQGAYIYVCGDAKGMARDVH 1314
>BI967853
Length = 666
Score = 28.5 bits (62), Expect = 6.0
Identities = 13/42 (30%), Positives = 21/42 (49%)
Frame = +2
Query: 73 CDRGMLARGVSVGTFFCGTYFDSEQVVDVVCLKNLDSLLHAM 114
C ++ RG+ + T F + ++CL NL LLHA+
Sbjct: 380 CSYSLIGRGILIXX*LAHTRFGRKLPTFLLCLNNLYLLLHAL 505
>TC228141 similar to GB|AAL77730.1|18700240|AY078029 AT3g54810/F28P10_210
{Arabidopsis thaliana;} , partial (23%)
Length = 1143
Score = 28.5 bits (62), Expect = 6.0
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +1
Query: 300 SRHGHASYPHAGTYLQGSSKLGIGVRNDAAKSNFIL 335
+ H H + H L KLG+ +RN AA+ + +L
Sbjct: 229 THHHHLEFSHYNNPLHSVDKLGMRMRNSAARVSLLL 336
>TC232084 UP|Q84PP2 (Q84PP2) Transcription factor MYB101 (Fragment), complete
Length = 1383
Score = 28.1 bits (61), Expect = 7.8
Identities = 14/51 (27%), Positives = 23/51 (44%), Gaps = 13/51 (25%)
Frame = -2
Query: 109 SLLHAMPNLNQIHALIEHYGPTVY-------------FHPDEKYMPSSVSW 146
S LH + L+ HA + Y P ++ FH ++ PSS++W
Sbjct: 191 STLHVLEVLSNSHAHVSEYNPPIFYPLLGSKAPSSNHFHHCMEFSPSSLTW 39
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.138 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,756,240
Number of Sequences: 63676
Number of extensions: 313815
Number of successful extensions: 1357
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 1343
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1353
length of query: 425
length of database: 12,639,632
effective HSP length: 100
effective length of query: 325
effective length of database: 6,272,032
effective search space: 2038410400
effective search space used: 2038410400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC135461.10