BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135234.2 - phase: 0 /pseudo
(1380 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BU549069 188 1e-47
BQ272766 weakly similar to GP|28558781|gb| pol protein {Cucumis ... 168 1e-41
TC213413 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, parti... 74 3e-13
TC211627 74 6e-13
TC211973 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, parti... 62 1e-09
NP595172 polyprotein [Glycine max] 61 3e-09
TC213114 weakly similar to UP|Q8W150 (Q8W150) Polyprotein, parti... 58 3e-08
TC212592 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, parti... 53 8e-07
BM731326 weakly similar to GP|21740635|em OSJNBb0043H09.2 {Oryza... 51 4e-06
TC234828 40 0.005
BE807407 33 0.87
CO982196 32 2.5
TC232772 weakly similar to UP|Q9LQH2 (Q9LQH2) F15O4.13, partial ... 31 3.3
CA784792 similar to PIR|T06782|T067 extensin - soybean, partial ... 30 7.3
BU578866 30 7.3
>BU549069
Length = 615
Score = 188 bits (478), Expect = 1e-47
Identities = 89/132 (67%), Positives = 108/132 (81%)
Frame = -1
Query: 1239 LRVTPMTGVGRALKSKKLTPKFIGPYQISERVGTVAYRVGLPPHLSNLHDVFHVSQLRKY 1298
L+VTP TGVG+ALKS+KLTP FIG +QI +R VAY++ LPP LSNLH+VFHVSQLR Y
Sbjct: 615 LKVTPRTGVGQALKSRKLTPHFIGHFQILKRAXPVAYQIALPPSLSNLHNVFHVSQLRMY 436
Query: 1299 VPDPSHVIHKGDVQVRDNLTVETLPLRIDDRKVKSLRGKEIPLVRVVWGGATGESLTWEL 1358
+ DPSHV+ DVQV++NLT ETLPLRI+DR+ K LR KE PLV+V+WGG +GE TWEL
Sbjct: 435 IHDPSHVVKLDDVQVKENLTYETLPLRIEDRRTKHLRRKENPLVKVIWGGTSGEDATWEL 256
Query: 1359 ESKMRESYPELF 1370
ES+MR +YP LF
Sbjct: 255 ESQMRVAYPSLF 220
>BQ272766 weakly similar to GP|28558781|gb| pol protein {Cucumis melo}, partial
(9%)
Length = 410
Score = 168 bits (426), Expect = 1e-41
Identities = 81/122 (66%), Positives = 100/122 (81%)
Frame = -3
Query: 1234 GDHVFLRVTPMTGVGRALKSKKLTPKFIGPYQISERVGTVAYRVGLPPHLSNLHDVFHVS 1293
GDHVFLRVTP TGVGRALKS KLTP FIGP+QI ++V VAY++ LPP L++LH+VFHVS
Sbjct: 366 GDHVFLRVTP*TGVGRALKS*KLTPHFIGPFQILKKVDFVAYQIALPPSLTSLHNVFHVS 187
Query: 1294 QLRKYVPDPSHVIHKGDVQVRDNLTVETLPLRIDDRKVKSLRGKEIPLVRVVWGGATGES 1353
Q+ KY+ DPSH+++ VQV++NL ETLPLRI D + K LRGKEI LV+V+WGGA+GE
Sbjct: 186 QIHKYINDPSHMVNLDVVQVKENLAHETLPLRIKDMRTKHLRGKEILLVKVIWGGASGEE 7
Query: 1354 LT 1355
T
Sbjct: 6 AT 1
>TC213413 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, partial (5%)
Length = 761
Score = 74.3 bits (181), Expect = 3e-13
Identities = 45/134 (33%), Positives = 66/134 (48%), Gaps = 1/134 (0%)
Frame = +1
Query: 1234 GDHVFLRVTP-MTGVGRALKSKKLTPKFIGPYQISERVGTVAYRVGLPPHLSNLHDVFHV 1292
GD V +++ P G KLT ++ GP+++ ER+G V YR+ L H S +H VFHV
Sbjct: 7 GDWVLVKLRPHRQGSASETTYSKLTKRYYGPFEVQERLGKVVYRLKLTAH-SRIHPVFHV 183
Query: 1293 SQLRKYVPDPSHVIHKGDVQVRDNLTVETLPLRIDDRKVKSLRGKEIPLVRVVWGGATGE 1352
S L+ +V DP H G + V PL + D K+ +V V W A+ +
Sbjct: 184 SLLKAFVGDP-ETTHAGPLPVMRTEEATNTPLTVIDSKLVPADNGPRRMVLVQWPSASRQ 360
Query: 1353 SLTWELESKMRESY 1366
+WE +RE Y
Sbjct: 361 DASWEDWQVLRERY 402
>TC211627
Length = 1034
Score = 73.6 bits (179), Expect = 6e-13
Identities = 47/134 (35%), Positives = 69/134 (51%), Gaps = 1/134 (0%)
Frame = +3
Query: 1234 GDHVFLRVTPMTGVGRALKSKKLTPKFIGPYQISERVGTVAYRVGLPPHLSNLHDVFHVS 1293
GD V++R+ P KL+ +F GPYQI RVG VAYR+ LPP S +H +FHVS
Sbjct: 429 GDWVYVRL*PYRQTSIQSTYTKLSKRFYGPYQIQARVGQVAYRLQLPP-TSKIHPIFHVS 605
Query: 1294 QLR-KYVPDPSHVIHKGDVQVRDNLTVETLPLRIDDRKVKSLRGKEIPLVRVVWGGATGE 1352
L+ + P P ++ ++ V+ PL+ D K+ IP V V W E
Sbjct: 606 LLKVHHGPIPPELLALPPFSTTNHPLVQ--PLQFLDWKMDESTTPPIPQVLVQWTNLAPE 779
Query: 1353 SLTWELESKMRESY 1366
TWE +++++ Y
Sbjct: 780 DTTWESWTQLKDIY 821
>TC211973 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, partial (4%)
Length = 730
Score = 62.4 bits (150), Expect = 1e-09
Identities = 31/75 (41%), Positives = 50/75 (66%), Gaps = 1/75 (1%)
Frame = +1
Query: 1234 GDHVFLRVTPMTGVGRALK-SKKLTPKFIGPYQISERVGTVAYRVGLPPHLSNLHDVFHV 1292
GD VFL++ P A + ++KL+P+F P+Q+ +VGT+AY++ LP H+ +H VFHV
Sbjct: 145 GD*VFLKMQPYRRRSLAKRINEKLSPRFYAPFQVFNKVGTIAYKLDLPSHI-KIHPVFHV 321
Query: 1293 SQLRKYVPDPSHVIH 1307
S L+K P+ H+ +
Sbjct: 322 SLLKKASPNLYHLCY 366
>NP595172 polyprotein [Glycine max]
Length = 4659
Score = 61.2 bits (147), Expect = 3e-09
Identities = 41/143 (28%), Positives = 69/143 (47%), Gaps = 9/143 (6%)
Frame = +1
Query: 1234 GDHVFLRVTPMTGVGRAL-KSKKLTPKFIGPYQISERVGTVAYRVGLPPHLSNLHDVFHV 1292
GD V +++ P L K++KL+ ++ GP+++ ++G VAY++ L P + +H VFHV
Sbjct: 4063 GDEVLVKLQPYRQHSAVLRKNQKLSMRYFGPFKVLAKIGDVAYKLEL-PSAARIHPVFHV 4239
Query: 1293 SQLR--------KYVPDPSHVIHKGDVQVRDNLTVETLPLRIDDRKVKSLRGKEIPLVRV 1344
SQL+ Y+P P V G V P++I ++ +I + V
Sbjct: 4240 SQLKPFNGTAQDPYLPLPLTVTEMGPVM---------QPVKILASRIIIRGHNQIEQILV 4392
Query: 1345 VWGGATGESLTWELESKMRESYP 1367
W + TWE ++ SYP
Sbjct: 4393 QWENGLQDEATWEDIEDIKASYP 4461
Score = 53.9 bits (128), Expect = 5e-07
Identities = 58/202 (28%), Positives = 94/202 (45%)
Frame = +2
Query: 464 WKTCLIRSL*NRVSRRGERLCCW*RRRMVA*DCA*TTVS*TKLR*RTGIRFRGLTT*WIS 523
+K C ++ N+V+ C RR+M D A * + *R + + ++
Sbjct: 1850 YKKCWFKASFNQVTVHFPYPYCLLRRKMEVGDSAQIIEL*MQSL*RIAFQCQQWMNC*MN 2029
Query: 524 WLVQRFLVRLT*GQVTIR*R*RMKICRRRTLGHVVVITNTR*CLSVLLMRQVYLWSIRTE 583
++ *GQ TI+ ++I R+ L H++ I N *C VLLM Q + + T+
Sbjct: 2030 YMELNIFQNWI*GQDTIKFWYSLRIERKLLLEHIMAIMNG**CHLVLLMHQPHSNA**TK 2209
Query: 584 FFMLFWIDSWWCLLMIF*FILRLKKSMPSI*GLYYRF*KRKSCMLSCRNVNSG*VK*VFS 643
+F L + + C LM *+I+ L + + S L YR * S +L C NV+ K +
Sbjct: 2210 YFSLLSENLFLCFLMTS*YIVLLGRIISSTWNLCYRP*SNISYLLDCPNVHLEIQKLITW 2389
Query: 644 ATLFLVVVLLLILRKLMQYPIG 665
AT +L +++ QY IG
Sbjct: 2390 ATRYLG*EFPWRIQRYKQY*IG 2455
Score = 40.8 bits (94), Expect = 0.004
Identities = 44/187 (23%), Positives = 80/187 (42%)
Frame = +3
Query: 987 CRSFCLCMFDLSEV*S*TSEACRVVDTIRCARVEVGQHFYGFYDEFTEYSDRA*CYLGFC 1046
C+S M DLS * A R T + +G+ +GF+ FT++ C+ G
Sbjct: 3324 CQSLYTEMLDLSTS*IKQYTASRTTPTFTYSSTSLGRCSHGFHHRFTKFL-WIICHNGSH 3500
Query: 1047 G*VDEVSALYSDKYQFSGTSAGGDLHPCHCEVAWCSVEYCVG*RSEVHF*ILEEFARCFG 1106
* ++ + +S K ++ H ++ W + Y V R + L F +
Sbjct: 3501 **THQICSFHSIKS*L*Q*GGSRGIYESHSQITWDTKIYSV*SR*GFYQYFLATFIQVTR 3680
Query: 1107 FEIEAEFGLSSSDRWSVGEDDTIFRGSTESVCT*ARWSLG*SPTVDRVHIQQ*LPFEYWY 1166
+ I LSS+ RW++ + +F + + * LG T+ RV + + +E +
Sbjct: 3681 YYIGHVISLSSTVRWTI*GAE*MFGDVS*MLHV*TS*RLGKGITMGRVLV*HCISYELRH 3860
Query: 1167 DTVRSFV 1173
D+++S V
Sbjct: 3861 DSIQSLV 3881
>TC213114 weakly similar to UP|Q8W150 (Q8W150) Polyprotein, partial (7%)
Length = 810
Score = 58.2 bits (139), Expect = 3e-08
Identities = 31/70 (44%), Positives = 46/70 (65%), Gaps = 1/70 (1%)
Frame = +3
Query: 1231 VSRGDHVFLRVTPMTGVGRALK-SKKLTPKFIGPYQISERVGTVAYRVGLPPHLSNLHDV 1289
+S GD V+L++ P A K ++KL+P+F GPYQI +++G VA+ + LPP +H V
Sbjct: 42 LSVGDWVYLKLQPYRLKSLAKKRNEKLSPRFYGPYQIKKQIGLVAFELDLPP-ARKIHPV 218
Query: 1290 FHVSQLRKYV 1299
FH S L+K V
Sbjct: 219 FHASLLKKAV 248
>TC212592 weakly similar to UP|Q84ZV5 (Q84ZV5) Polyprotein, partial (4%)
Length = 664
Score = 53.1 bits (126), Expect = 8e-07
Identities = 37/103 (35%), Positives = 55/103 (52%)
Frame = +2
Query: 1255 KLTPKFIGPYQISERVGTVAYRVGLPPHLSNLHDVFHVSQLRKYVPDPSHVIHKGDVQVR 1314
KL+ +F GP+Q+ ERVG VAYR+ LP + LH VFH S L+ + +P +
Sbjct: 74 KLSKRFFGPFQVVERVGKVAYRLQLPVD-AKLHPVFHCSLLKPFQGNPPDTAAPLPPTLF 250
Query: 1315 DNLTVETLPLRIDDRKVKSLRGKEIPLVRVVWGGATGESLTWE 1357
D+ +V PL I +++ +I V V W G + + TWE
Sbjct: 251 DHQSV-IAPLVI--LATRTVNDDDIE-VLVQWQGLSPDDATWE 367
>BM731326 weakly similar to GP|21740635|em OSJNBb0043H09.2 {Oryza sativa
(japonica cultivar-group)}, partial (3%)
Length = 424
Score = 50.8 bits (120), Expect = 4e-06
Identities = 27/68 (39%), Positives = 42/68 (61%), Gaps = 1/68 (1%)
Frame = +1
Query: 1231 VSRGDHVFLRVTP-MTGVGRALKSKKLTPKFIGPYQISERVGTVAYRVGLPPHLSNLHDV 1289
++ GD V+L++ P G KLT +F GPY + +VG VA+++ LP + +H V
Sbjct: 85 INVGDWVYLKIRPHRQGSMPPRLHPKLTARFYGPYLVMRQVGAVAFQLQLPSE-ARIHPV 261
Query: 1290 FHVSQLRK 1297
FHVSQL++
Sbjct: 262 FHVSQLKR 285
>TC234828
Length = 857
Score = 40.4 bits (93), Expect = 0.005
Identities = 29/87 (33%), Positives = 41/87 (46%)
Frame = +1
Query: 397 LCRWRIRL*LTDYR**MNSLRCFLMRSLMCHRRGR*SFQLTLFQERSRCRWHHIVCQHRS 456
LC W+ L L YR N + F M + CH R + + LT R +CR I C +
Sbjct: 595 LCMWKRTLKLVVYRWCQNFQKFFRMMFVNCHLREKWNSLLTWCLGRIQCRLRLIECLRWN 774
Query: 457 WLN*RNSWKTCLIRSL*NRVSRRGERL 483
*R+ ++T +L + RGERL
Sbjct: 775 *QR*RHKYRTF*ANNLFVQAHHRGERL 855
>BE807407
Length = 341
Score = 33.1 bits (74), Expect = 0.87
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = +2
Query: 1255 KLTPKFIGPYQISERVGTVAYRVGLPPH 1282
KLT ++ GPY + +VG VA+++ LP H
Sbjct: 158 KLTARYYGPYLVLIKVGVVAFQLQLPKH 241
>CO982196
Length = 812
Score = 31.6 bits (70), Expect = 2.5
Identities = 29/92 (31%), Positives = 39/92 (41%)
Frame = +3
Query: 991 CLCMFDLSEV*S*TSEACRVVDTIRCARVEVGQHFYGFYDEFTEYSDRA*CYLGFCG*VD 1050
C CM +LSE * * CR T + V + YGF+ T+ S R Y+ G* D
Sbjct: 492 CCCM*NLSEK*D*YIIPCRAAITTAYSN*GVD*YLYGFHWRLTK-SSREGQYISGSG*AD 668
Query: 1051 EVSALYSDKYQFSGTSAGGDLHPCHCEVAWCS 1082
V + + K + +H E AW S
Sbjct: 669 *VCSFFCIKSPLYS*GSS*VIH*RIGETAWFS 764
>TC232772 weakly similar to UP|Q9LQH2 (Q9LQH2) F15O4.13, partial (7%)
Length = 729
Score = 31.2 bits (69), Expect = 3.3
Identities = 14/48 (29%), Positives = 27/48 (56%)
Frame = +1
Query: 1252 KSKKLTPKFIGPYQISERVGTVAYRVGLPPHLSNLHDVFHVSQLRKYV 1299
+ KL P+ GP+Q+ ER+ AY++ +P + F+++ L +V
Sbjct: 322 RMSKLQPRGDGPFQVLERINYNAYKIDIPGEY-EVSSSFNIADLTPFV 462
>CA784792 similar to PIR|T06782|T067 extensin - soybean, partial (11%)
Length = 435
Score = 30.0 bits (66), Expect = 7.3
Identities = 13/46 (28%), Positives = 28/46 (60%)
Frame = -2
Query: 555 GHVVVITNTR*CLSVLLMRQVYLWSIRTEFFMLFWIDSWWCLLMIF 600
G ++V+ R*C+ V L+ + + W + +F L+W WW +++++
Sbjct: 173 GMLIVVRRGR*CIDVRLLVR-WWWRVVVVWFFLWW--GWW*VVVVW 45
>BU578866
Length = 409
Score = 30.0 bits (66), Expect = 7.3
Identities = 17/58 (29%), Positives = 27/58 (46%)
Frame = +2
Query: 1283 LSNLHDVFHVSQLRKYVPDPSHVIHKGDVQVRDNLTVETLPLRIDDRKVKSLRGKEIP 1340
LSN+HDV S L + P V DV N ++++ L R ++ L + +P
Sbjct: 212 LSNMHDVVSTSSLDLALVKPLPVSFPSDVSTGKNTNIDSVQLTQATRPIRRLYLENLP 385
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.369 0.167 0.626
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,397,691
Number of Sequences: 63676
Number of extensions: 942256
Number of successful extensions: 11621
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 4547
Number of HSP's successfully gapped in prelim test: 410
Number of HSP's that attempted gapping in prelim test: 6823
Number of HSP's gapped (non-prelim): 5502
length of query: 1380
length of database: 12,639,632
effective HSP length: 109
effective length of query: 1271
effective length of database: 5,698,948
effective search space: 7243362908
effective search space used: 7243362908
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.7 bits)
S2: 65 (29.6 bits)
Medicago: description of AC135234.2