BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC134822.4 - phase: 0
(290 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC205709 weakly similar to UP|O45549 (O45549) C. elegans NUD-1 p... 371 e-103
TC205708 weakly similar to UP|O35685 (O35685) MNUDC protein (Nuc... 280 7e-76
TC229080 72 2e-13
TC205710 similar to UP|Q8L5X1 (Q8L5X1) Fimbrin-like protein (Act... 65 5e-11
BE023042 similar to SP|P26585|HMGL HMG1/2-like protein (SB11 pro... 38 0.006
TC220935 30 1.3
TC222447 similar to UP|O48933 (O48933) Histidinol dehydrogenase ... 30 1.3
TC234331 weakly similar to UP|Q96316 (Q96316) Blue-copper bingin... 29 2.9
TC209347 28 3.7
CA853409 similar to GP|16209702|gb| AT3g58970/F17J16_20 {Arabido... 28 3.7
CF922383 28 3.7
TC216791 similar to UP|O23144 (O23144) Proton pump interactor (A... 28 3.7
TC234145 similar to UP|Q84L08 (Q84L08) GONST4 Golgi Nucleotide s... 28 4.9
TC224710 similar to UP|Q8ZZP6 (Q8ZZP6) Conserved protein, partia... 28 4.9
TC217799 similar to UP|O23173 (O23173) Nucleoporin-like protein,... 28 4.9
TC227846 weakly similar to UP|Q947K4 (Q947K4) Thiohydroximate S-... 28 4.9
TC227442 homologue to GB|CAF05883.1|40804623|BX908789 related to... 28 4.9
BE612319 28 4.9
TC218503 28 6.4
BU765568 similar to GP|2194119|gb|A F20P5.5 gene product {Arabid... 28 6.4
>TC205709 weakly similar to UP|O45549 (O45549) C. elegans NUD-1 protein
(Corresponding sequence F53A2.4), partial (31%)
Length = 1291
Score = 371 bits (953), Expect = e-103
Identities = 191/304 (62%), Positives = 230/304 (74%), Gaps = 14/304 (4%)
Frame = +2
Query: 1 MAIISDYQDETQTPSSSSQPKP-----SKTIPFSSTFDPSKPTAFLEKVFDFIAKESTDF 55
MAIISD+ +E Q P+ SS P+P S + FS+ FD S P AFLE+VF F+++ S DF
Sbjct: 41 MAIISDFNEEEQKPTHSSPPQPQSSSSSSSSSFSAAFDRSNPIAFLERVFQFVSEHS-DF 217
Query: 56 FDKDSAEKMVLSAVRAAKVKKAKAVAAEKAKI---------ASQEKAKAAAGIKVNDEKS 106
+SAEK + VRAA KK + + +E+ K AS+E+ K+ +K E
Sbjct: 218 LATESAEKEIALLVRAAGKKKREFLKSEREKAEKKKREDLKASEEREKSDKRLKEEKEPK 397
Query: 107 GVGTEKKDGESGLAAPNQGNGMDLEKYSWTQTLQELNVNVPVPNGTKSGFVICEIKKNHL 166
T K + S + PN+GNG+DLEKYSWTQ+LQE+NVNVPVPNGTKS FV EIKKNHL
Sbjct: 398 AEDTSKDESTSKV--PNKGNGLDLEKYSWTQSLQEVNVNVPVPNGTKSRFVTVEIKKNHL 571
Query: 167 KVGLKGQPPIIDGELYKFIKADECYWSIEDQSTVSILLTKHDQMDWWKCLVKGDPLINTQ 226
KVGLKGQPPII+GE YK +K D+CYWSIEDQ+ +SILLTKHDQM+WWKCLVKGDP I+TQ
Sbjct: 572 KVGLKGQPPIIEGEFYKSVKPDDCYWSIEDQNAISILLTKHDQMEWWKCLVKGDPEIDTQ 751
Query: 227 KVEPESSKLGELDSETRMTVEKMMFDQRQKSMGLPTSEELEKQEMMKKFMSQHPNMDFSG 286
KVEPE+SKLG+LD ETR TVEKMMFDQRQKSMGLPTSEEL+KQEM+KKFMS+HP MDFS
Sbjct: 752 KVEPENSKLGDLDPETRQTVEKMMFDQRQKSMGLPTSEELQKQEMLKKFMSEHPEMDFSR 931
Query: 287 AKLS 290
AK+S
Sbjct: 932 AKIS 943
>TC205708 weakly similar to UP|O35685 (O35685) MNUDC protein (Nuclear
distribution gene C homolog) (Sig 92 protein) (Mus
musculus 10 days neonate cerebellum cDNA, RIKEN
full-length enriched library, clone:6530413H12
product:nuclear distribution gene C homolog
(Aspergillus), full inser, partial (15%)
Length = 757
Score = 280 bits (715), Expect = 7e-76
Identities = 143/248 (57%), Positives = 181/248 (72%), Gaps = 12/248 (4%)
Frame = +3
Query: 1 MAIISDYQDETQTPSSSSQPKP---SKTIPFSSTFDPSKPTAFLEKVFDFIAKESTDFFD 57
MAIISD+ +E P+ SS P+P S + FS+TFD S P AFLE+VF+F++++S DF
Sbjct: 21 MAIISDFNEEEPKPAHSSPPQPQSSSSSSSFSATFDRSNPIAFLERVFEFVSEQS-DFLA 197
Query: 58 KDSAEKMVLSAVRAAKVKKAKAVAAEKAKI---------ASQEKAKAAAGIKVNDEKSGV 108
SAEK ++S V AA KK + + AE+ ++E+ K ++N+EK
Sbjct: 198 TQSAEKEIVSLVHAAGKKKRELLKAERENADKKKREDLKVTEERQKVEK--RLNEEKELK 371
Query: 109 GTEKKDGESGLAAPNQGNGMDLEKYSWTQTLQELNVNVPVPNGTKSGFVICEIKKNHLKV 168
+ ES PN+GNG+DLEKYSWTQ+LQE+NVNVPVPNGTKS FV EIKKNHLKV
Sbjct: 372 AEDTNKNESASRVPNKGNGLDLEKYSWTQSLQEVNVNVPVPNGTKSRFVTVEIKKNHLKV 551
Query: 169 GLKGQPPIIDGELYKFIKADECYWSIEDQSTVSILLTKHDQMDWWKCLVKGDPLINTQKV 228
GLKGQPPII+GE +K +K D+CYWSIEDQ+++SILLTKHDQM+WWKCLVKGDP I+TQKV
Sbjct: 552 GLKGQPPIIEGEFHKSVKPDDCYWSIEDQNSISILLTKHDQMEWWKCLVKGDPEIDTQKV 731
Query: 229 EPESSKLG 236
EPE+SKLG
Sbjct: 732 EPENSKLG 755
>TC229080
Length = 792
Score = 72.4 bits (176), Expect = 2e-13
Identities = 44/159 (27%), Positives = 74/159 (45%), Gaps = 1/159 (0%)
Frame = +3
Query: 133 YSWTQTLQELNVNVPVPNGTKSGFVICEIKKNHLKVGLKGQPPIIDGELYKFIKADECYW 192
+ W QTL E+N+ + +P S C+I+ HL++G+KG PP ++ +L +K D +W
Sbjct: 150 FEWDQTLDEVNIYITLPPNVHSKQFYCKIQSKHLELGIKGNPPYLNHDLTSPVKTDSSFW 329
Query: 193 SIEDQSTVSILLTKHDQMDWWKCLVKGDPLINTQKVEPESSKLGELDSETRMTVEKMMFD 252
++ED + I L K D+ W + G G+LD
Sbjct: 330 TLED-DIMHITLQKRDKGQTWASPILGQ---------------GQLD------------- 422
Query: 253 QRQKSMGLPTSEELE-KQEMMKKFMSQHPNMDFSGAKLS 290
P + +LE K+ M+++F ++P DFS A+ S
Sbjct: 423 --------PYATDLEQKRLMLQRFQEENPGFDFSQAQFS 515
>TC205710 similar to UP|Q8L5X1 (Q8L5X1) Fimbrin-like protein (Actin binding
motif), partial (11%)
Length = 714
Score = 64.7 bits (156), Expect = 5e-11
Identities = 45/129 (34%), Positives = 70/129 (53%), Gaps = 10/129 (7%)
Frame = +2
Query: 6 DYQDETQTPSSSSQPKP-SKTIPFSSTFDPSKPTAFLEKVFDFIAKESTDFFDKDSAEKM 64
++ +E Q P+ SS P+P S + FS TFD S P FL +VF+F++ E +DF + SAEK
Sbjct: 53 NFNEEEQKPAHSSPPQPQSSSSSFSGTFDMSNPITFLVRVFEFVS-EHSDFLETKSAEKE 229
Query: 65 VLSAVRAAKVKKAKAVAAEKAKI---------ASQEKAKAAAGIKVNDEKSGVGTEKKDG 115
V S V A + KK + + E+ K+ AS+E+ K+ +K +EK +
Sbjct: 230 VASLVCAVE-KKREFLKVEREKVEKKKREDLKASKERGKSDKRLK--EEKKSKAEDTNKD 400
Query: 116 ESGLAAPNQ 124
+S PN+
Sbjct: 401 KSTSRVPNK 427
>BE023042 similar to SP|P26585|HMGL HMG1/2-like protein (SB11 protein).
[Soybean] {Glycine max}, partial (84%)
Length = 388
Score = 37.7 bits (86), Expect = 0.006
Identities = 35/101 (34%), Positives = 46/101 (44%), Gaps = 1/101 (0%)
Frame = +2
Query: 33 DPSKPTAFLEKVFDFIAKESTDFFDKDSAEKMVLSAV-RAAKVKKAKAVAAEKAKIASQE 91
DPSKP VFDFI +E F K+ E + AV ++A+ K AEKA ++
Sbjct: 116 DPSKPKRPASAVFDFI-EEFRKVFSKEHPENKAVCAVGKSARTKWKTMSDAEKAPYVAES 292
Query: 92 KAKAAAGIKVNDEKSGVGTEKKDGESGLAAPNQGNGMDLEK 132
+ + KV EKS KK E P G+ D EK
Sbjct: 293 EKR-----KVEFEKSIRAYIKKQAE----GPTGGDEEDSEK 388
>TC220935
Length = 669
Score = 30.0 bits (66), Expect = 1.3
Identities = 13/31 (41%), Positives = 19/31 (60%)
Frame = -3
Query: 147 PVPNGTKSGFVICEIKKNHLKVGLKGQPPII 177
P+P+GT G I E+K +K+G K P I+
Sbjct: 376 PLPSGTLHGGCILEVKLRQIKIGRKLLPSIV 284
>TC222447 similar to UP|O48933 (O48933) Histidinol dehydrogenase (Fragment),
partial (39%)
Length = 656
Score = 30.0 bits (66), Expect = 1.3
Identities = 22/63 (34%), Positives = 27/63 (41%), Gaps = 1/63 (1%)
Frame = +1
Query: 4 ISDYQDETQTPSSSSQPKPSKTIPFSSTFDPSKPTA-FLEKVFDFIAKESTDFFDKDSAE 62
I+ Y+ TPS K I FSS F P + K D KE T FDK +
Sbjct: 115 INSYRLSNLTPSELQSLKSRPRIDFSSIFSVVNPIVDDVHKRGDAAVKEYTSKFDKVELD 294
Query: 63 KMV 65
K+V
Sbjct: 295 KVV 303
>TC234331 weakly similar to UP|Q96316 (Q96316) Blue-copper binging protein
III (Uclacyanin 3), partial (13%)
Length = 710
Score = 28.9 bits (63), Expect = 2.9
Identities = 19/42 (45%), Positives = 25/42 (59%), Gaps = 3/42 (7%)
Frame = +1
Query: 11 TQTPSSSSQP--KPSKTIPFSSTF-DPSKPTAFLEKVFDFIA 49
T +PSSSS P PS ++P SSTF + S PT L + F +
Sbjct: 208 TPSPSSSSSPYPPPSSSLPPSSTFTNKSPPTQTLALSYYFFS 333
>TC209347
Length = 640
Score = 28.5 bits (62), Expect = 3.7
Identities = 30/114 (26%), Positives = 47/114 (40%), Gaps = 7/114 (6%)
Frame = +3
Query: 10 ETQTPSSSSQPKPSKTIPFSSTFDPSKPTAFLEKVFDFIAKESTDFF-DKDSAEKMVLSA 68
E+ P+S P+P+ + DP +E F D+ D++SAE+ +
Sbjct: 228 ESSEPASPDAPEPAAPV------DP------VELAFSKANAYKADYNNDENSAEERNVGD 371
Query: 69 VRAAKVKKAKAVAAEKAKIASQEKAKAA------AGIKVNDEKSGVGTEKKDGE 116
+ + +A EKAK Q KA AA + V++ SG K GE
Sbjct: 372 ETPKDLPDSVKIAIEKAKKYKQNKAVAAVTETTQGSLGVSERSSGKNKVGKKGE 533
>CA853409 similar to GP|16209702|gb| AT3g58970/F17J16_20 {Arabidopsis
thaliana}, partial (16%)
Length = 542
Score = 28.5 bits (62), Expect = 3.7
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = +1
Query: 12 QTPSSSSQPKPSKTIPFSSTFDPSKPTAFLEK 43
+TPSS++Q P +T F + P+ PT+ LE+
Sbjct: 349 RTPSSATQRFPLETCEFWAPSSPTPPTSSLER 444
>CF922383
Length = 691
Score = 28.5 bits (62), Expect = 3.7
Identities = 24/98 (24%), Positives = 46/98 (46%), Gaps = 4/98 (4%)
Frame = +1
Query: 178 DGELYKFIKADECYWSIE---DQSTVSILLTKHDQMDWWKCLVKGDPLINTQKVEPESSK 234
D +F++A E +E + T S+ ++H+ +KG+ + +K+E K
Sbjct: 409 DSPSREFLEAQEKIQELEVELQRLTESLKTSEHENDQ-----LKGEISVTKEKLEESGKK 573
Query: 235 LGELDSETRMTVEKMMFDQRQKSMGLPTSEE-LEKQEM 271
ELD + E+++ + + + L T EE L QE+
Sbjct: 574 YEELDLSHKKLQEQILEPENKYNQQLSTLEEALXSQEV 687
>TC216791 similar to UP|O23144 (O23144) Proton pump interactor
(AT4g27500/F27G19_100), partial (25%)
Length = 1385
Score = 28.5 bits (62), Expect = 3.7
Identities = 36/130 (27%), Positives = 50/130 (37%), Gaps = 19/130 (14%)
Frame = +3
Query: 11 TQTPSSSSQPKPSKTIPFSSTFDPSKPTAFLEKVFDFIAK-ESTDFFDKDSAE------- 62
T+ P +P P +T+P SK + K D +K ES D + D E
Sbjct: 435 TKQPKEEPKPSPQETLPAQKE---SKKRS-KNKGRDLKSKPESKDVAETDEYEFKNPQKE 602
Query: 63 ----------KMVLSAVRAAKVKKAKAVAAEKAKIASQEKAKAAAGIKVNDEKSGVGTEK 112
+ R ++ KAK K K+A + AKAA + EK EK
Sbjct: 603 APAKEPEIDPAKLKEMKREEEIAKAKQALERKKKLAEKAAAKAAIRAQKEAEKKLKDREK 782
Query: 113 K-DGESGLAA 121
K +SG AA
Sbjct: 783 KAKKKSGTAA 812
>TC234145 similar to UP|Q84L08 (Q84L08) GONST4 Golgi Nucleotide sugar
transporter (Fragment), partial (29%)
Length = 442
Score = 28.1 bits (61), Expect = 4.9
Identities = 18/44 (40%), Positives = 20/44 (44%)
Frame = +1
Query: 11 TQTPSSSSQPKPSKTIPFSSTFDPSKPTAFLEKVFDFIAKESTD 54
T TPS S PK S P SST PS PT + S+D
Sbjct: 307 TTTPSPSPPPKNSSPPPSSST-SPSSPTPIFSAMPTSTPSSSSD 435
>TC224710 similar to UP|Q8ZZP6 (Q8ZZP6) Conserved protein, partial (5%)
Length = 787
Score = 28.1 bits (61), Expect = 4.9
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = -2
Query: 14 PSSSSQPKPSKTIPFSSTFDP 34
P+SSS PKP+ TIP + T P
Sbjct: 153 PNSSSTPKPTPTIPNTCTHHP 91
>TC217799 similar to UP|O23173 (O23173) Nucleoporin-like protein, partial
(23%)
Length = 538
Score = 28.1 bits (61), Expect = 4.9
Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 2/35 (5%)
Frame = +2
Query: 11 TQTPSSSSQPKPSKTIPFSSTFD--PSKPTAFLEK 43
T PSSSSQP + +P + P KP+ FL++
Sbjct: 230 TIAPSSSSQPTTASIVPVFDFYSGLPRKPSPFLQQ 334
>TC227846 weakly similar to UP|Q947K4 (Q947K4) Thiohydroximate
S-glucosyltransferase, partial (49%)
Length = 1605
Score = 28.1 bits (61), Expect = 4.9
Identities = 12/37 (32%), Positives = 19/37 (50%)
Frame = +2
Query: 155 GFVICEIKKNHLKVGLKGQPPIIDGELYKFIKADECY 191
GF+ +K HL + + G PP+ L F++ E Y
Sbjct: 413 GFIQLPVKMEHLPLRVPGLPPLDSRALPSFVRFPESY 523
>TC227442 homologue to GB|CAF05883.1|40804623|BX908789 related to prefoldin
subunit 3 {Neurospora crassa;} , partial (6%)
Length = 595
Score = 28.1 bits (61), Expect = 4.9
Identities = 20/63 (31%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Frame = +1
Query: 52 STDFFDKDSAEKMVLSAVRAAK-VKKAKAVAAEKAKIASQEKAKAAAGIKVNDEKSGVGT 110
++D ++K+ EK + ++K + AKAVA+ A +KAK AA + KSG
Sbjct: 160 ASDHYEKEEEEKTKMKKSGSSKKMADAKAVAS-----AGMDKAKTAAVVGAQKVKSGTSA 324
Query: 111 EKK 113
K
Sbjct: 325 GLK 333
>BE612319
Length = 398
Score = 28.1 bits (61), Expect = 4.9
Identities = 19/67 (28%), Positives = 33/67 (48%), Gaps = 6/67 (8%)
Frame = +2
Query: 15 SSSSQPKPSKTIPFSSTFDPSKPTAFLE-----KVFDFIA-KESTDFFDKDSAEKMVLSA 68
S S P P T P ST P+ PT+ + +VF + +E ++D+ +K+V S
Sbjct: 119 SLSPSPSPILTDPLLSTSSPATPTSLEDAYEALRVFQILGIEEKNTPVNEDTCQKVVESL 298
Query: 69 VRAAKVK 75
++ +K
Sbjct: 299 ASSSPLK 319
>TC218503
Length = 640
Score = 27.7 bits (60), Expect = 6.4
Identities = 19/46 (41%), Positives = 26/46 (56%), Gaps = 6/46 (13%)
Frame = +2
Query: 3 IISDYQDETQT--PSSSSQPKPSKTIPFSS----TFDPSKPTAFLE 42
I S +Q++TQT S S PSKT+ SS TFD +P+ L+
Sbjct: 206 IASAFQEQTQTLQQQSLSSECPSKTVSNSSTDETTFDFERPSKLLK 343
>BU765568 similar to GP|2194119|gb|A F20P5.5 gene product {Arabidopsis
thaliana}, partial (6%)
Length = 421
Score = 27.7 bits (60), Expect = 6.4
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +1
Query: 13 TPSSSSQPKPSKTIPFSSTFDPSKPTAFLEKVFD 46
TPS S P PS + PF P P+A EK ++
Sbjct: 259 TPSPSPSPSPSPSTPFP---HPPSPSAEKEKTYE 351
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.310 0.127 0.355
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,533,983
Number of Sequences: 63676
Number of extensions: 133984
Number of successful extensions: 794
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 776
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 788
length of query: 290
length of database: 12,639,632
effective HSP length: 96
effective length of query: 194
effective length of database: 6,526,736
effective search space: 1266186784
effective search space used: 1266186784
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC134822.4