Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC134521.1 - phase: 0 /pseudo
         (324 letters)

Database: GMGI 
           63,676 sequences; 37,918,896 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC222199                                                              118  3e-27
TC216808                                                               59  9e-20
BI317622                                                               56  3e-08
BE611176                                                               39  3e-05
BG508280                                                               45  4e-05
TC223279                                                               32  0.51
TC223682 homologue to UP|SYE_ARATH (Q9FEA2) Glutamyl-tRNA synthe...    31  0.67
TC214657 similar to UP|Q9XGV6 (Q9XGV6) Bacterial-induced peroxid...    29  2.5
BM086125                                                               29  2.5
TC213622                                                               29  3.3
TC216967                                                               28  7.4
TC221598 homologue to GB|AAM19966.1|20466089|AY098956 At2g32600/...    28  7.4
TC215322 similar to UP|Q9LSL3 (Q9LSL3) Oligopeptidase A, partial...    27  9.6
TC221584 weakly similar to UP|Q9FH22 (Q9FH22) Gb|AAF17687.1, par...    27  9.6

>TC222199 
          Length = 691

 Score =  118 bits (296), Expect = 3e-27
 Identities = 79/229 (34%), Positives = 117/229 (50%), Gaps = 22/229 (9%)
 Frame = +2

Query: 75  DFQLFPTLEEYSHWVGLPVLDEVPFHAFGPIP---KIPTIAKALHLEVADIKDKFTTKDG 131
           DFQL PT+EE+   +G P+ +  P+ + G +P   +IPT+ K     +  +K    T++G
Sbjct: 5   DFQLVPTIEEFEEILGCPLGERKPYLSSGCLPSLSRIPTMVKDSARGLDRVKQ---TRNG 175

Query: 132 LPCLPYNFLHQKATTCFEKSKTDDFESILALLIYGIVLFPKVDKFVDMNAIRIFLT---- 187
           +  LP  +L  KA     +     F  +LALLI+G+VLFP VD  VD+ AI  FL     
Sbjct: 176 IAGLPRKYLEDKARGIANQGDWVPFMDVLALLIFGVVLFPNVDGLVDLAAIDAFLAYHHS 355

Query: 188 -QNPVPVLLADTYVAIHDRTSHGKGTIVCCTPLLHRWITSHL-PRPSIRPEP-------- 237
            ++PV  +LAD +   + R       I+CC P L  W+ SHL  + + RP P        
Sbjct: 356 KESPVVAVLADLFDTFNRRYEKSSARIICCLPALCVWLVSHLFQQDTRRPCPLLSHRSCT 535

Query: 238 ----IPWSQKLMTWTPKDIIWFNPSCD-PEFIIDSCGDFNNVPLLGTQG 281
               I W Q L     + I WF    +  E ++ SCG + N+PL+GT+G
Sbjct: 536 EKRRIDWDQLLAGIGGRTISWFPRWKEGKEGVLFSCGRYPNIPLVGTRG 682


>TC216808 
          Length = 1144

 Score = 58.9 bits (141), Expect(2) = 9e-20
 Identities = 38/141 (26%), Positives = 68/141 (47%)
 Frame = +2

Query: 6   RRTHGYKFKAPDTESLEGLRGLTKMLKNPGHFQERYGNLLDILKTKVDVMLLNTLVQFYD 65
           +R +  K K+ +  SL  L  L   L+    F++ YG + D+ + +V +  + +L Q+YD
Sbjct: 146 KRFYQVKIKSLEVTSLGELGQLMGQLQR*A-FRKAYGKIWDLARVEVSMEPIASLSQYYD 322

Query: 66  PIYHGFTFPDFQLFPTLEEYSHWVGLPVLDEVPFHAFGPIPKIPTIAKALHLEVADIKDK 125
                FTF DFQL PT++E     G  +    P+   G  P    +AK + +   ++   
Sbjct: 323 QPLRCFTFEDFQLVPTVKELEEIRGCQLGGRKPYLFSGFYPSTTRVAKVVKISAQELNRV 502

Query: 126 FTTKDGLPCLPYNFLHQKATT 146
              ++G+  +P   L ++A T
Sbjct: 503 KQNRNGVVGVPRKCLEERAKT 565



 Score = 55.5 bits (132), Expect(2) = 9e-20
 Identities = 35/83 (42%), Positives = 49/83 (58%), Gaps = 7/83 (8%)
 Frame = +3

Query: 156 FESILALLIYGIVLFPKVDKFVDMNAIRIFLT-----QNPVPVLLA--DTYVAIHDRTSH 208
           F  ILALLI+G+VLFP ++  VD+ AI  FL      ++PV  +LA  DT+    +++S 
Sbjct: 594 FIDILALLIFGVVLFPNMEGLVDLAAIDAFLAFHHGKESPVVAILAMYDTFDRGCEKSS- 770

Query: 209 GKGTIVCCTPLLHRWITSHLPRP 231
               IVCC   L+ W+ SHL  P
Sbjct: 771 --ARIVCCALALYVWLVSHLFSP 833


>BI317622 
          Length = 370

 Score = 55.8 bits (133), Expect = 3e-08
 Identities = 40/123 (32%), Positives = 54/123 (43%), Gaps = 19/123 (15%)
 Frame = -2

Query: 174 DKFVDMNAIRIFLTQN-----PVPVLLADTYVAIHDRTSHGKGTIVCCTPLLHRWITSHL 228
           D  VD+ AI  FL  +     P+  +LAD Y   + R       IVCCTP L+ W+ SHL
Sbjct: 369 DGLVDLAAIGAFLAYHDYKEIPIVAMLADLYDTFNRRCEKSSTRIVCCTPALYVWLVSHL 190

Query: 229 PRPSIRPE-PIP------------WSQKLMTWTPKDIIWFNPSCDPEF-IIDSCGDFNNV 274
            R  +R   P+             W Q L       + WF    +    ++ SCG F NV
Sbjct: 189 FRQEVRHACPLESHCSCTEKGGENWGQLLANKEGASVNWFPRWKEGRTRVLISCGVFLNV 10

Query: 275 PLL 277
           PL+
Sbjct: 9   PLM 1


>BE611176 
          Length = 424

 Score = 38.9 bits (89), Expect(2) = 3e-05
 Identities = 24/74 (32%), Positives = 38/74 (50%)
 Frame = -2

Query: 5   GRRTHGYKFKAPDTESLEGLRGLTKMLKNPGHFQERYGNLLDILKTKVDVMLLNTLVQFY 64
           G+R +  K K  D  S++ L  L   L+    F++ Y  +LD+   ++    + +L Q+Y
Sbjct: 363 GKRFYQVKIKGLDITSIKELGRLMGPLQMQA-FRKAYEKILDLTIAEISTEAIASLTQYY 187

Query: 65  DPIYHGFTFPDFQL 78
           D     FTF DFQL
Sbjct: 186 D*PLRCFTFGDFQL 145



 Score = 26.2 bits (56), Expect(2) = 3e-05
 Identities = 11/33 (33%), Positives = 18/33 (54%)
 Frame = -1

Query: 80  PTLEEYSHWVGLPVLDEVPFHAFGPIPKIPTIA 112
           PT+EE+   +G P+    P+   G +P +  IA
Sbjct: 142 PTVEEFEEILGCPLRGRKPYFFSGFLPSLSKIA 44


>BG508280 
          Length = 418

 Score = 45.1 bits (105), Expect = 4e-05
 Identities = 19/33 (57%), Positives = 26/33 (78%)
 Frame = +1

Query: 264 IIDSCGDFNNVPLLGTQGGISYSPILARRQFEY 296
           ++ SCG F NVPL+GT+G ISY+P+LA +Q  Y
Sbjct: 70  VLISCGGFPNVPLMGTRGCISYNPVLAIKQLGY 168


>TC223279 
          Length = 539

 Score = 31.6 bits (70), Expect = 0.51
 Identities = 17/44 (38%), Positives = 23/44 (51%)
 Frame = -3

Query: 173 VDKFVDMNAIRIFLTQNPVPVLLADTYVAIHDRTSHGKGTIVCC 216
           ++KF    AI+IFLT    P  +A  Y+       HGK  I+CC
Sbjct: 447 LNKFDRSEAIQIFLTSTFDPFHIATLYLHRGP*HLHGKNCIICC 316


>TC223682 homologue to UP|SYE_ARATH (Q9FEA2) Glutamyl-tRNA synthetase
           (Glutamate--tRNA ligase) (GluRS) , partial (19%)
          Length = 461

 Score = 31.2 bits (69), Expect = 0.67
 Identities = 18/63 (28%), Positives = 25/63 (39%), Gaps = 2/63 (3%)
 Frame = -2

Query: 203 HDRTSHGKGTIVCCTPLLHRWITSHLP--RPSIRPEPIPWSQKLMTWTPKDIIWFNPSCD 260
           HDR   G   IVC           H P    ++ P  +   + L  W P+   W +PS  
Sbjct: 436 HDRIGVGSPHIVCIKNSSQTAYKDHNPLQHQALHPNQVQAKKDL*AWPPQTPYWTSPSQC 257

Query: 261 PEF 263
           P+F
Sbjct: 256 PQF 248


>TC214657 similar to UP|Q9XGV6 (Q9XGV6) Bacterial-induced peroxidase
           precursor  , partial (72%)
          Length = 1750

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 16/43 (37%), Positives = 19/43 (43%)
 Frame = +3

Query: 203 HDRTSHGKGTIVCCTPLLHRWITSHLPRPSIRPEPIPWSQKLM 245
           H    H K  + C     HRW   H P PS  P P P  Q+L+
Sbjct: 369 HQLRHHQKSQLPC-----HRW--EHQPGPSRHPHPQPLRQQLL 476


>BM086125 
          Length = 428

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 18/55 (32%), Positives = 22/55 (39%), Gaps = 7/55 (12%)
 Frame = -2

Query: 203 HDRTSHGKGTIVCCTPLLHR---WITSHLPR----PSIRPEPIPWSQKLMTWTPK 250
           H     G+ T+ CC     R   WI    P     PS+ P+P  W  KL    PK
Sbjct: 196 HRNEERGRNTLRCCCCYRWR*LHWIPIQAPLALLLPSLLPQPRCWCSKLSL*APK 32


>TC213622 
          Length = 607

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 12/31 (38%), Positives = 18/31 (57%)
 Frame = +3

Query: 224 ITSHLPRPSIRPEPIPWSQKLMTWTPKDIIW 254
           + S +P PS  P P+  +  L T+TPK  +W
Sbjct: 174 VHSRIPMPSFPPSPL--TSALSTFTPKPAVW 260


>TC216967 
          Length = 1117

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 18/68 (26%), Positives = 32/68 (46%), Gaps = 7/68 (10%)
 Frame = +1

Query: 66  PIYHGFTFPDFQLFPTLEEYSHWVGLPVLDEVP----FHAFGPIPKIPTIAKALHLEVAD 121
           P+ +   FP++  FPTL ++  + G+P     P    F+     P   TIA  +    +D
Sbjct: 124 PLANPAGFPEYYYFPTL-QFEPFGGMPFFTHAPPPAMFYPVAETPLTNTIANQIDYYFSD 300

Query: 122 ---IKDKF 126
              +KD++
Sbjct: 301 ANLVKDEY 324


>TC221598 homologue to GB|AAM19966.1|20466089|AY098956 At2g32600/T26B15.16
           {Arabidopsis thaliana;} , partial (64%)
          Length = 542

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 14/47 (29%), Positives = 21/47 (43%), Gaps = 3/47 (6%)
 Frame = -1

Query: 239 PWSQKLMTWTPKDIIWFN---PSCDPEFIIDSCGDFNNVPLLGTQGG 282
           PW ++L  W      WF    P C+P      CG+ + V +   +GG
Sbjct: 275 PWPRELHVWPS----WFGGAFPECEPSSCPRCCGE*DRVCIRSFRGG 147


>TC215322 similar to UP|Q9LSL3 (Q9LSL3) Oligopeptidase A, partial (56%)
          Length = 1338

 Score = 27.3 bits (59), Expect = 9.6
 Identities = 20/72 (27%), Positives = 31/72 (42%), Gaps = 3/72 (4%)
 Frame = -1

Query: 142 QKATTCFEKSKTDDFESILAL---LIYGIVLFPKVDKFVDMNAIRIFLTQNPVPVLLADT 198
           Q+A+    +  +  F++ L      +YG   FP  +K    +       Q P P L    
Sbjct: 741 QQASNILLQGDSPQFQNALQCPLKFLYGNTSFP*TEKVTQQHPTLSL*YQQPDPHLA**A 562

Query: 199 YVAIHDRTSHGK 210
           YV +HD+  HGK
Sbjct: 561 YVEVHDQI-HGK 529


>TC221584 weakly similar to UP|Q9FH22 (Q9FH22) Gb|AAF17687.1, partial (35%)
          Length = 694

 Score = 27.3 bits (59), Expect = 9.6
 Identities = 14/40 (35%), Positives = 21/40 (52%)
 Frame = -3

Query: 51  KVDVMLLNTLVQFYDPIYHGFTFPDFQLFPTLEEYSHWVG 90
           K+ + L +TL     P+Y   +FP   LFP  + +S  VG
Sbjct: 311 KICLHLQSTLQNALSPLYM*MSFPGKSLFPQRKHWSRKVG 192


  Database: GMGI
    Posted date:  Oct 22, 2004  4:58 PM
  Number of letters in database: 37,918,896
  Number of sequences in database:  63,676
  
Lambda     K      H
   0.324    0.144    0.458 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,655,657
Number of Sequences: 63676
Number of extensions: 289422
Number of successful extensions: 1378
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 1363
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1373
length of query: 324
length of database: 12,639,632
effective HSP length: 97
effective length of query: 227
effective length of database: 6,463,060
effective search space: 1467114620
effective search space used: 1467114620
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 59 (27.3 bits)


Medicago: description of AC134521.1