BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC134049.5 + phase: 0
(162 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CF922226 108 9e-25
TC226202 similar to UP|O81126 (O81126) 9G8-like SR protein (RSZp... 40 5e-04
TC226201 similar to UP|O81126 (O81126) 9G8-like SR protein (RSZp... 40 5e-04
TC226200 similar to UP|O81126 (O81126) 9G8-like SR protein (RSZp... 40 5e-04
TC216231 similar to UP|Q41188 (Q41188) Glycine-rich protein 2 (G... 39 0.001
TC206178 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, part... 39 0.001
TC206177 similar to UP|Q41188 (Q41188) Glycine-rich protein 2 (G... 39 0.001
TC223754 similar to UP|Q86EQ4 (Q86EQ4) Clone ZZD1536 mRNA sequen... 38 0.002
CO984791 38 0.002
TC206180 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, part... 36 0.009
TC219583 weakly similar to UP|GRP2_NICSY (P27484) Glycine-rich p... 35 0.012
TC206176 weakly similar to UP|Q8LPA7 (Q8LPA7) Cold shock protein... 35 0.016
TC208012 similar to UP|Q761Z7 (Q761Z7) BRI1-KD interacting prote... 35 0.020
TC210743 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1,... 34 0.027
CO984873 34 0.035
TC227588 similar to PIR|T00837|T00837 glycine-rich protein T13L1... 33 0.059
TC231744 32 0.10
TC217411 similar to UP|Q8L7S8 (Q8L7S8) At5g26740, partial (26%) 31 0.23
AW203350 31 0.30
CO982951 31 0.30
>CF922226
Length = 667
Score = 108 bits (271), Expect = 9e-25
Identities = 68/184 (36%), Positives = 105/184 (56%), Gaps = 28/184 (15%)
Frame = -3
Query: 2 EQLAEFNKILDDLENIEVQLEDEDKAILLLCALPRSFESFKDTMLYGKEGTVTLEEVQAA 61
EQL FNK++ DLENI+V ++DED+A+LLLC LP+S+ FK+T+L+G++ +V+L+EVQ A
Sbjct: 584 EQLDLFNKLILDLENIDVTIDDEDQALLLLCYLPKSYSHFKETLLFGRD-SVSLDEVQTA 408
Query: 62 LRTKELTKSKDLRADEDNEGLSVSKGNGGGRGNRGSSK----------SGNKDKYKCFKC 111
L +KEL + K+ ++ EGL+ ++G + + K GN K +C+ C
Sbjct: 407 LNSKELNERKEKKSSASGEGLT-ARGKTFKKDSEFDKKKQKPENQKNGEGNIFKIRCYHC 231
Query: 112 HKLGHFKRDCPE------------DNESSAQVVSEEYGDAGALVVS------CWEEDEGE 153
K GH ++ CPE D+ ++A V + Y A AL+VS W D G
Sbjct: 230 KKEGHTRKVCPERQKNGGSNNRKKDSGNAAIVQDDGYESAEALMVSEKNPETKWIMDSGC 51
Query: 154 GSHL 157
H+
Sbjct: 50 SWHM 39
>TC226202 similar to UP|O81126 (O81126) 9G8-like SR protein (RSZp22 splicing
factor), partial (72%)
Length = 1003
Score = 40.0 bits (92), Expect = 5e-04
Identities = 19/37 (51%), Positives = 24/37 (64%)
Frame = +3
Query: 85 SKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDC 121
S+G GGGRG R SG D KC++C + GHF R+C
Sbjct: 780 SRGGGGGRGGR----SGGSD-LKCYECGEPGHFAREC 875
>TC226201 similar to UP|O81126 (O81126) 9G8-like SR protein (RSZp22 splicing
factor), partial (72%)
Length = 820
Score = 40.0 bits (92), Expect = 5e-04
Identities = 19/37 (51%), Positives = 24/37 (64%)
Frame = +1
Query: 85 SKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDC 121
S+G GGGRG R SG D KC++C + GHF R+C
Sbjct: 193 SRGGGGGRGGR----SGGSD-LKCYECGEPGHFAREC 288
>TC226200 similar to UP|O81126 (O81126) 9G8-like SR protein (RSZp22 splicing
factor), partial (89%)
Length = 936
Score = 40.0 bits (92), Expect = 5e-04
Identities = 19/37 (51%), Positives = 24/37 (64%)
Frame = +2
Query: 85 SKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDC 121
S+G GGGRG R SG D KC++C + GHF R+C
Sbjct: 284 SRGGGGGRGGR----SGGSD-LKCYECGEPGHFAREC 379
>TC216231 similar to UP|Q41188 (Q41188) Glycine-rich protein 2 (GRP2)
(AT4g38680/F20M13_240), partial (42%)
Length = 891
Score = 38.9 bits (89), Expect = 0.001
Identities = 24/74 (32%), Positives = 30/74 (40%)
Frame = +3
Query: 87 GNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDCPEDNESSAQVVSEEYGDAGALVVSC 146
G GGG G G G C+KC + GH RDC + + YG G SC
Sbjct: 183 GGGGGYGGGGGGGGGG-----CYKCGETGHIARDCSQGGGGGGR-----YGGGGGGGGSC 332
Query: 147 WEEDEGEGSHLGID 160
+ + GE H D
Sbjct: 333 Y--NCGESGHFARD 368
Score = 36.6 bits (83), Expect = 0.005
Identities = 16/36 (44%), Positives = 19/36 (52%)
Frame = +3
Query: 87 GNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDCP 122
G GGGR G G+ C+ C + GHF RDCP
Sbjct: 282 GGGGGRYGGGGGGGGS-----CYNCGESGHFARDCP 374
>TC206178 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (69%)
Length = 1138
Score = 38.9 bits (89), Expect = 0.001
Identities = 22/62 (35%), Positives = 26/62 (41%)
Frame = +2
Query: 85 SKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDCPEDNESSAQVVSEEYGDAGALVV 144
S G GGG G RG G +D C C +LGH RDC ++ G G L
Sbjct: 776 SGGGGGGGGARGGGGGGYRDVV-CRNCQQLGHMSRDC-----MGPLMICHNCGGRGHLAY 937
Query: 145 SC 146
C
Sbjct: 938 EC 943
Score = 29.6 bits (65), Expect = 0.66
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = +2
Query: 108 CFKCHKLGHFKRDCPED 124
C C K GH RDCP D
Sbjct: 650 CNNCRKTGHLARDCPND 700
Score = 26.2 bits (56), Expect = 7.3
Identities = 10/28 (35%), Positives = 14/28 (49%)
Frame = +2
Query: 95 RGSSKSGNKDKYKCFKCHKLGHFKRDCP 122
R S+ G C C + GH+ R+CP
Sbjct: 305 RRDSRRGFSRDNLCKNCKRPGHYARECP 388
>TC206177 similar to UP|Q41188 (Q41188) Glycine-rich protein 2 (GRP2)
(AT4g38680/F20M13_240), partial (22%)
Length = 406
Score = 38.5 bits (88), Expect = 0.001
Identities = 18/49 (36%), Positives = 22/49 (44%)
Frame = +1
Query: 74 RADEDNEGLSVSKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDCP 122
RA+ G + GGG G G G C+ C + GHF RDCP
Sbjct: 4 RAEFARGGGGGGRYGGGGGGRYGGGGGGGGGGGSCYSCGESGHFARDCP 150
>TC223754 similar to UP|Q86EQ4 (Q86EQ4) Clone ZZD1536 mRNA sequence, partial
(22%)
Length = 742
Score = 38.1 bits (87), Expect = 0.002
Identities = 17/37 (45%), Positives = 18/37 (47%)
Frame = +2
Query: 87 GNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDCPE 123
G GGR G G K CF C K GHF R+C E
Sbjct: 359 GMEGGRFGGGGGSGGGGGKSTCFNCGKPGHFARECVE 469
Score = 32.7 bits (73), Expect = 0.078
Identities = 14/31 (45%), Positives = 18/31 (57%)
Frame = +2
Query: 91 GRGNRGSSKSGNKDKYKCFKCHKLGHFKRDC 121
G+GN G SG CF+C ++GH RDC
Sbjct: 278 GKGNIGGGGSGGG----CFRCGEVGHLARDC 358
Score = 30.8 bits (68), Expect = 0.30
Identities = 14/36 (38%), Positives = 17/36 (46%)
Frame = +2
Query: 86 KGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDC 121
+G GG G G G+ CF+C GH RDC
Sbjct: 179 RGGGGSVGIGGGGGGGS-----CFRCGGFGHMARDC 271
Score = 29.6 bits (65), Expect = 0.66
Identities = 14/37 (37%), Positives = 15/37 (39%)
Frame = +2
Query: 85 SKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDC 121
S +GGG G G CF C GH RDC
Sbjct: 92 SSNSGGGGGGSGGG---------CFNCGGFGHLARDC 175
Score = 26.9 bits (58), Expect = 4.3
Identities = 12/38 (31%), Positives = 17/38 (44%)
Frame = +2
Query: 90 GGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDCPEDNES 127
G R N G + C++C + GH RDC + S
Sbjct: 11 GARRNGGGGAA-------CYQCGEFGHLARDCNRSSNS 103
>CO984791
Length = 672
Score = 38.1 bits (87), Expect = 0.002
Identities = 31/126 (24%), Positives = 60/126 (47%), Gaps = 6/126 (4%)
Frame = -3
Query: 2 EQLAEFNKILDDLENIEVQLEDEDKAILLLCALPRSFESFKDTMLYGKEGTVTLEEVQAA 61
E + E + + L++++++L ++ L+L +LP F FK + K+ E +
Sbjct: 556 EYIIEMSNLASKLKSLKLELGEDLLMYLVLISLPAHFGQFKVSYNT*KDKWSLNELISHC 377
Query: 62 LRTKEL-----TKSKDLRADEDNEGLSVSKGNGGGRGNRGSSKSGNKDK-YKCFKCHKLG 115
++ +E T+S L + N+ +KG G + K NKD+ + C+ K G
Sbjct: 376 VQEEESL*RDRTESAHLISTSQNKKWKKTKGAA*GTS*Q---KKQNKDEEFACYFYKKSG 206
Query: 116 HFKRDC 121
H K++C
Sbjct: 205 HMKKEC 188
>TC206180 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (42%)
Length = 655
Score = 35.8 bits (81), Expect = 0.009
Identities = 20/58 (34%), Positives = 24/58 (40%)
Frame = +3
Query: 89 GGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDCPEDNESSAQVVSEEYGDAGALVVSC 146
GGG G RG G +D C C +LGH RDC ++ G G L C
Sbjct: 264 GGGGGARGGGGGGYRDVV-CRNCQQLGHMSRDC-----MGPLMICHNCGGRGHLAYEC 419
Score = 29.6 bits (65), Expect = 0.66
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = +3
Query: 108 CFKCHKLGHFKRDCPED 124
C C K GH RDCP D
Sbjct: 135 CNNCRKTGHLARDCPND 185
>TC219583 weakly similar to UP|GRP2_NICSY (P27484) Glycine-rich protein 2,
partial (45%)
Length = 995
Score = 35.4 bits (80), Expect = 0.012
Identities = 15/46 (32%), Positives = 22/46 (47%), Gaps = 10/46 (21%)
Frame = +2
Query: 86 KGNGGGRGNRGSSKSGNKDKYK----------CFKCHKLGHFKRDC 121
+G GGGRG G G + + + C+ C ++GH RDC
Sbjct: 329 RGGGGGRGRGGGRYGGGEGRGRGFGRRGGGPECYNCGRIGHLARDC 466
Score = 35.0 bits (79), Expect = 0.016
Identities = 16/39 (41%), Positives = 21/39 (53%), Gaps = 3/39 (7%)
Frame = +2
Query: 87 GNGGGRGNRGSSK---SGNKDKYKCFKCHKLGHFKRDCP 122
G GGG G+ G ++ G CF C + GHF R+CP
Sbjct: 473 GQGGGGGDDGRNRRRGGGGGGGGGCFNCGEEGHFARECP 589
>TC206176 weakly similar to UP|Q8LPA7 (Q8LPA7) Cold shock protein-1, partial
(77%)
Length = 883
Score = 35.0 bits (79), Expect = 0.016
Identities = 16/36 (44%), Positives = 18/36 (49%)
Frame = +3
Query: 87 GNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDCP 122
G GGG G GS C+ C + GHF RDCP
Sbjct: 558 GGGGGGGGGGS----------CYSCGESGHFARDCP 635
Score = 31.6 bits (70), Expect = 0.17
Identities = 14/35 (40%), Positives = 16/35 (45%)
Frame = +3
Query: 87 GNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDC 121
G GGG G G G C+ C + GH RDC
Sbjct: 384 GGGGGYGGGGRGGGGGA----CYNCGESGHLARDC 476
>TC208012 similar to UP|Q761Z7 (Q761Z7) BRI1-KD interacting protein 117
(Fragment), partial (38%)
Length = 993
Score = 34.7 bits (78), Expect = 0.020
Identities = 17/57 (29%), Positives = 28/57 (48%), Gaps = 1/57 (1%)
Frame = +1
Query: 68 TKSKDLRADE-DNEGLSVSKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDCPE 123
+ K ++ +E +E ++ +G G G + K + C+KC K GH RDC E
Sbjct: 13 SSGKSIKKEETSSENDTLDQGKKPGSGPSDAPKVPSDAPKICYKCKKAGHLSRDCKE 183
>TC210743 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(53%)
Length = 452
Score = 34.3 bits (77), Expect = 0.027
Identities = 23/85 (27%), Positives = 36/85 (42%)
Frame = +3
Query: 33 ALPRSFESFKDTMLYGKEGTVTLEEVQAALRTKELTKSKDLRADEDNEGLSVSKGNGGGR 92
A+ + ++ L G+ TV + Q + + ++ D D + G GGGR
Sbjct: 240 AMDEAIDAMNGMDLDGRTITVDRAQPQQGSTRDDGDRYRERGRDRDRD-----HGGGGGR 404
Query: 93 GNRGSSKSGNKDKYKCFKCHKLGHF 117
G+ G +CFKC K GHF
Sbjct: 405 GSNGG---------ECFKCGKPGHF 452
>CO984873
Length = 754
Score = 33.9 bits (76), Expect = 0.035
Identities = 14/38 (36%), Positives = 23/38 (59%)
Frame = -2
Query: 84 VSKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDC 121
+ KG+ + ++K K++ KCF C+K GH K+DC
Sbjct: 429 MKKGDQAKNKGKITAKPVIKNESKCFFCNKKGHIKKDC 316
>TC227588 similar to PIR|T00837|T00837 glycine-rich protein T13L16.11 -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(10%)
Length = 1300
Score = 33.1 bits (74), Expect = 0.059
Identities = 15/41 (36%), Positives = 25/41 (60%), Gaps = 2/41 (4%)
Frame = +1
Query: 108 CFKCHKLGHFKRDCPEDNESSAQ--VVSEEYGDAGALVVSC 146
CF C K GH +DCPE + S+++ + + G++G + SC
Sbjct: 184 CFICKKGGHRAKDCPEKHTSTSKSIAICLKCGNSGHDIFSC 306
Score = 28.1 bits (61), Expect = 1.9
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = +1
Query: 108 CFKCHKLGHFKRDCPEDNES 127
CFKC + GHF R+C +S
Sbjct: 505 CFKCGEEGHFARECTSSIKS 564
>TC231744
Length = 794
Score = 32.3 bits (72), Expect = 0.10
Identities = 26/122 (21%), Positives = 54/122 (43%)
Frame = +2
Query: 2 EQLAEFNKILDDLENIEVQLEDEDKAILLLCALPRSFESFKDTMLYGKEGTVTLEEVQAA 61
E + E + + L++++++L ++ L+L +LP F FK + K+ +L E+ +
Sbjct: 92 EYIMEISNLASKLKSLKLELGEDLFVHLVLISLPAHFGQFKVSYNTQKDKW-SLNELISH 268
Query: 62 LRTKELTKSKDLRADEDNEGLSVSKGNGGGRGNRGSSKSGNKDKYKCFKCHKLGHFKRDC 121
+E +D N+ +K K +++ C+ C K H K+ C
Sbjct: 269 CVQEEERL*RDRTESAHNKKRKKTKDVA--EKTS*QKKQQKDEEFTCYFCKKSRHMKKKC 442
Query: 122 PE 123
P+
Sbjct: 443 PK 448
>TC217411 similar to UP|Q8L7S8 (Q8L7S8) At5g26740, partial (26%)
Length = 1275
Score = 31.2 bits (69), Expect = 0.23
Identities = 13/32 (40%), Positives = 19/32 (58%), Gaps = 2/32 (6%)
Frame = +3
Query: 93 GNRGSSKSGNKDKY--KCFKCHKLGHFKRDCP 122
G+R SS+ + D++ CF C + GH DCP
Sbjct: 765 GSRRSSRPSSSDRFGGACFNCGESGHRASDCP 860
>AW203350
Length = 411
Score = 30.8 bits (68), Expect = 0.30
Identities = 10/15 (66%), Positives = 12/15 (79%)
Frame = +1
Query: 108 CFKCHKLGHFKRDCP 122
C+KC K GH+ RDCP
Sbjct: 16 CYKCGKPGHWSRDCP 60
>CO982951
Length = 757
Score = 30.8 bits (68), Expect = 0.30
Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 2/40 (5%)
Frame = -3
Query: 86 KGNGGGRGNRGSSKSGNKDKYK--CFKCHKLGHFKRDCPE 123
KG G + N S + K + C C K GHF++DCP+
Sbjct: 296 KGKGPLKINNNSKQIQKKTSKRNNCHFCGKSGHFQKDCPK 177
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.310 0.132 0.373
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,038,503
Number of Sequences: 63676
Number of extensions: 68881
Number of successful extensions: 538
Number of sequences better than 10.0: 83
Number of HSP's better than 10.0 without gapping: 497
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 532
length of query: 162
length of database: 12,639,632
effective HSP length: 90
effective length of query: 72
effective length of database: 6,908,792
effective search space: 497433024
effective search space used: 497433024
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC134049.5