Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC133139.2 + phase: 0 /pseudo
         (155 letters)

Database: GMGI 
           63,676 sequences; 37,918,896 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC212986                                                               50  6e-07
BM108247 weakly similar to PIR|H84710|H847 Mutator-like transpos...    37  0.003
BF425842                                                               36  0.007
TC228067                                                               32  0.12
TC228324 UP|LGUL_SOYBN (P46417) Lactoylglutathione lyase  (Methy...    32  0.16
TC210267 similar to UP|Q8LGU6 (Q8LGU6) HOBBIT protein, partial (...    30  0.47
TC228281 similar to UP|BIOB_ARATH (P54967) Biotin synthase  (Bio...    30  0.61
TC206861                                                               28  1.4
TC205889 UP|Q9FQE9 (Q9FQE9) Glutathione S-transferase GST 9  , c...    28  1.8
BQ273732 similar to GP|8978266|dbj| anthocyanidin-3-glucoside rh...    27  3.0
TC228023 similar to UP|Q9FLH2 (Q9FLH2) 2-oxoglutarate dehydrogen...    27  5.2
TC207488 similar to UP|Q9SJE0 (Q9SJE0) T27G7.21, partial (58%)         27  5.2
BI785278                                                               26  6.7
TC216101 similar to UP|Q9SCA1 (Q9SCA1) Calcium-binding protein, ...    26  8.8
BG509681 similar to EGAD|98465|10647 7 kDa photosystem ii core c...    26  8.8
TC215330 similar to UP|Q6T7D1 (Q6T7D1) Fiber protein Fb19 (Fragm...    26  8.8
TC221801 similar to UP|KHL1_MOUSE (Q9JI74) Kelch-like protein 1,...    26  8.8

>TC212986 
          Length = 404

 Score = 49.7 bits (117), Expect = 6e-07
 Identities = 22/53 (41%), Positives = 35/53 (65%), Gaps = 2/53 (3%)
 Frame = +1

Query: 87  HYRN--QIRDWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNNVLK 137
           H+R+   IR       +W+D  PK++W+Q  D+G+RWG +TTN+SE  N++ K
Sbjct: 1   HWRHLGDIRANKPSAAEWLDQLPKQKWVQCFDEGKRWG-LTTNLSESVNSMFK 156


>BM108247 weakly similar to PIR|H84710|H847 Mutator-like transposase
           [imported] - Arabidopsis thaliana, partial (4%)
          Length = 265

 Score = 37.4 bits (85), Expect = 0.003
 Identities = 15/55 (27%), Positives = 28/55 (50%)
 Frame = +2

Query: 76  SGYSMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISE 130
           + ++ T  +      +I + S +  KW+      QW   H +G R+GH+++NI E
Sbjct: 35  ASHATTTIAFKEKMGEIEEVSPEAAKWLQQFXPSQWALVHFKGTRFGHLSSNIEE 199


>BF425842 
          Length = 414

 Score = 36.2 bits (82), Expect = 0.007
 Identities = 18/47 (38%), Positives = 27/47 (57%), Gaps = 5/47 (10%)
 Frame = +3

Query: 10  WVTPQPDLGLISDRHESIKSVVRRLDSNWH-----HVFCIRHITQLF 51
           +VTPQP+L +ISDR   + + ++     W+      V+CIRHI   F
Sbjct: 246 YVTPQPNLCIISDRGTGLLATLQSKHVGWNGPDVSSVYCIRHIASNF 386


>TC228067 
          Length = 1716

 Score = 32.0 bits (71), Expect = 0.12
 Identities = 23/63 (36%), Positives = 29/63 (45%), Gaps = 9/63 (14%)
 Frame = +1

Query: 100 VKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNNV--LKG-----GPQSSNHTN--CPG 150
           V+WV D  +   LQ HD   R G      ++   +    KG     GP S NHT+  CPG
Sbjct: 490 VEWVKDQIRANGLQMHDNVLRKGINPRTRAQQLEDFRRFKGISHYEGPDSDNHTSLPCPG 669

Query: 151 NLL 153
            LL
Sbjct: 670 ELL 678


>TC228324 UP|LGUL_SOYBN (P46417) Lactoylglutathione lyase  (Methylglyoxalase)
           (Aldoketomutase) (Glyoxalase I) , complete
          Length = 995

 Score = 31.6 bits (70), Expect = 0.16
 Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 3/45 (6%)
 Frame = +1

Query: 79  SMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWL---QAHDQGRR 120
           S   PS  + R+Q R W D + K + D  KK WL   + H++G++
Sbjct: 313 SPLMPSDPYKRSQARFWVDYIDKKIYDTWKKMWLSKGEEHEEGKK 447


>TC210267 similar to UP|Q8LGU6 (Q8LGU6) HOBBIT protein, partial (39%)
          Length = 1273

 Score = 30.0 bits (66), Expect = 0.47
 Identities = 13/41 (31%), Positives = 23/41 (55%)
 Frame = -3

Query: 71   INFLPSGYSMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQW 111
            I+F P  +S   P++  YR+ +  W   V+  + ++PKK W
Sbjct: 1199 ISFSPIQFSSFGPALGIYRSFLAPWLHHVLLLICESPKKLW 1077


>TC228281 similar to UP|BIOB_ARATH (P54967) Biotin synthase  (Biotin
           synthetase) , partial (39%)
          Length = 677

 Score = 29.6 bits (65), Expect = 0.61
 Identities = 18/56 (32%), Positives = 25/56 (44%)
 Frame = +2

Query: 94  DWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNNVLKGGPQSSNHTNCP 149
           DWS D VK + D+P    L    Q  R  H    + +C    +K G  S + + CP
Sbjct: 299 DWSRDQVKSIYDSPILDLLFHGAQVHRHAHNFREVQQCTLLSIKTGGCSEDCSYCP 466


>TC206861 
          Length = 1326

 Score = 28.5 bits (62), Expect = 1.4
 Identities = 16/52 (30%), Positives = 24/52 (45%)
 Frame = -2

Query: 13   PQPDLGLISDRHESIKSVVRRLDSNWHHVFCIRHITQLFEDFQEHRDEEKSK 64
            P P L + S +      +V+   S WHH    +++    E  QE  + EKSK
Sbjct: 1016 PFPCLTIKSGKSTKASVMVQWTSSVWHHEVNTKNLQIKIESLQESHNLEKSK 861


>TC205889 UP|Q9FQE9 (Q9FQE9) Glutathione S-transferase GST 9  , complete
          Length = 1011

 Score = 28.1 bits (61), Expect = 1.8
 Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 3/41 (7%)
 Frame = +3

Query: 83  PSITHYRNQIRDWSDDVVKWVDDNPKKQWL---QAHDQGRR 120
           PS  + R Q R W D + K V D  +K WL   + H+ G++
Sbjct: 333 PSDPYERAQARFWVDYIDKKVYDTWRKMWLSKGEEHEAGKK 455


>BQ273732 similar to GP|8978266|dbj| anthocyanidin-3-glucoside
           rhamnosyltransferase-like {Arabidopsis thaliana},
           partial (3%)
          Length = 419

 Score = 27.3 bits (59), Expect = 3.0
 Identities = 17/56 (30%), Positives = 27/56 (47%), Gaps = 1/56 (1%)
 Frame = +2

Query: 8   RLWVTPQPDL-GLISDRHESIKSVVRRLDSNWHHVFCIRHITQLFEDFQEHRDEEK 62
           R +++PQP + GL     + + S +R  D+ W  +   RHI      F EH +  K
Sbjct: 80  RGFLSPQPFISGLTKSEEQQVLSKIRTQDA-WQILISERHIPAATCFFSEHAENSK 244


>TC228023 similar to UP|Q9FLH2 (Q9FLH2) 2-oxoglutarate dehydrogenase, E1
            component, partial (36%)
          Length = 1591

 Score = 26.6 bits (57), Expect = 5.2
 Identities = 15/44 (34%), Positives = 19/44 (43%), Gaps = 6/44 (13%)
 Frame = -3

Query: 112  LQAHDQGRRWGHITTNISECFNNVL------KGGPQSSNHTNCP 149
            L  H  G+ W +I TN   CF+N         G  Q  N T+ P
Sbjct: 1508 LPRHASGK-WNYIVTNYGRCFSNYTA*IKKKNGNSQGINSTSKP 1380


>TC207488 similar to UP|Q9SJE0 (Q9SJE0) T27G7.21, partial (58%)
          Length = 955

 Score = 26.6 bits (57), Expect = 5.2
 Identities = 11/44 (25%), Positives = 22/44 (50%), Gaps = 4/44 (9%)
 Frame = -3

Query: 95  WSDDVVKWVDD----NPKKQWLQAHDQGRRWGHITTNISECFNN 134
           WSD + +  ++    N     L+  +  R+WG ++  + EC+ N
Sbjct: 224 WSDSLGEGAEEQYATNGNADDLELEEMKRKWGCLSVKVEECWYN 93


>BI785278 
          Length = 422

 Score = 26.2 bits (56), Expect = 6.7
 Identities = 10/25 (40%), Positives = 13/25 (52%)
 Frame = -1

Query: 118 GRRWGHITTNISECFNNVLKGGPQS 142
           G+ WGH      +C    L+GGP S
Sbjct: 326 GQEWGHGLLPRIQCLTKQLQGGPNS 252


>TC216101 similar to UP|Q9SCA1 (Q9SCA1) Calcium-binding protein, partial
           (65%)
          Length = 796

 Score = 25.8 bits (55), Expect = 8.8
 Identities = 14/51 (27%), Positives = 23/51 (44%)
 Frame = +1

Query: 19  LISDRHESIKSVVRRLDSNWHHVFCIRHITQLFEDFQEHRDEEKSKKYGLN 69
           LI D+   +  ++ R+D N      +     L+E   E RDEE+  +   N
Sbjct: 325 LIPDK--DLAQMIERIDVNGDGCVDMDEFGDLYESIMEERDEEEDMREAFN 471


>BG509681 similar to EGAD|98465|10647 7 kDa photosystem ii core complex
          protein {Spinacia oleracea}, partial (72%)
          Length = 396

 Score = 25.8 bits (55), Expect = 8.8
 Identities = 10/18 (55%), Positives = 13/18 (71%)
 Frame = +1

Query: 74 LPSGYSMTQPSITHYRNQ 91
          LPS +S   PS+TH R+Q
Sbjct: 10 LPSAFSTPPPSLTHQRHQ 63


>TC215330 similar to UP|Q6T7D1 (Q6T7D1) Fiber protein Fb19 (Fragment),
          partial (94%)
          Length = 1021

 Score = 25.8 bits (55), Expect = 8.8
 Identities = 8/20 (40%), Positives = 15/20 (75%)
 Frame = -2

Query: 25 ESIKSVVRRLDSNWHHVFCI 44
          ES+++ +  +DSN HH+ C+
Sbjct: 66 ESVRAEITVVDSNHHHLLCL 7


>TC221801 similar to UP|KHL1_MOUSE (Q9JI74) Kelch-like protein 1, partial
           (3%)
          Length = 968

 Score = 25.8 bits (55), Expect = 8.8
 Identities = 8/15 (53%), Positives = 10/15 (66%)
 Frame = +1

Query: 35  DSNWHHVFCIRHITQ 49
           D+ WH  FC RHI +
Sbjct: 331 DAGWHCSFCFRHINE 375


  Database: GMGI
    Posted date:  Oct 22, 2004  4:58 PM
  Number of letters in database: 37,918,896
  Number of sequences in database:  63,676
  
Lambda     K      H
   0.318    0.135    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,479,410
Number of Sequences: 63676
Number of extensions: 145447
Number of successful extensions: 674
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 673
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 673
length of query: 155
length of database: 12,639,632
effective HSP length: 89
effective length of query: 66
effective length of database: 6,972,468
effective search space: 460182888
effective search space used: 460182888
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)


Medicago: description of AC133139.2