BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC131240.2 + phase: 0
(90 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ253804 similar to GP|10176854|dbj pectinacetylesterase {Arabid... 97 1e-21
TC204336 similar to UP|Q41695 (Q41695) Pectinacetylesterase prec... 64 2e-11
TC205391 similar to UP|Q8L7Z5 (Q8L7Z5) At1g57590/T8L23_6, partia... 63 2e-11
BM307860 61 1e-10
TC205388 similar to UP|Q8L7Z5 (Q8L7Z5) At1g57590/T8L23_6, partia... 57 1e-09
TC205392 57 1e-09
TC204335 homologue to UP|Q41695 (Q41695) Pectinacetylesterase pr... 55 8e-09
AW598043 similar to PIR|S68805|S688 pectin acetylesterase (EC 3.... 55 8e-09
TC204334 weakly similar to UP|Q41695 (Q41695) Pectinacetylestera... 54 1e-08
TC204338 weakly similar to UP|Q41695 (Q41695) Pectinacetylestera... 48 7e-07
BE610392 46 3e-06
BF324517 weakly similar to PIR|S68805|S688 pectin acetylesterase... 35 0.008
TC204615 similar to GB|AAL08296.1|15912325|AY056440 At1g60710/F8... 29 0.35
BE608416 weakly similar to PIR|T05825|T058 pectin acetylesterase... 29 0.46
TC230317 26 3.0
TC228350 similar to UP|Q75IA4 (Q75IA4) Expressed protein, partia... 26 3.9
TC224608 UP|VATL_PHAAU (O22552) Vacuolar ATP synthase 16 kDa pro... 25 5.0
TC204618 similar to UP|Q9ASZ9 (Q9ASZ9) At1g60730/F8A5_24, partia... 25 6.6
TC234824 similar to UP|Q9I7K2 (Q9I7K2) CG4655-PA, partial (4%) 25 8.6
CA784288 25 8.6
>BQ253804 similar to GP|10176854|dbj pectinacetylesterase {Arabidopsis
thaliana}, partial (15%)
Length = 423
Score = 97.1 bits (240), Expect = 1e-21
Identities = 48/68 (70%), Positives = 55/68 (80%), Gaps = 1/68 (1%)
Frame = +3
Query: 14 VAFALFYLITVESWRVHSQEPK-KLYVNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGE 72
+AFA L TVE WRV SQ+ + +L VNMTLV +AR +GA CLDGSLPAYHL RGFGAG+
Sbjct: 147 LAFAFLCLFTVEPWRVCSQQQQQRLLVNMTLVPSARASGALCLDGSLPAYHLHRGFGAGK 326
Query: 73 DNWLLQFE 80
DNWLLQFE
Sbjct: 327 DNWLLQFE 350
>TC204336 similar to UP|Q41695 (Q41695) Pectinacetylesterase precursor ,
partial (98%)
Length = 1592
Score = 63.5 bits (153), Expect = 2e-11
Identities = 28/43 (65%), Positives = 33/43 (76%)
Frame = +1
Query: 38 YVNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
YV +T+V NA GA CLDGS PAYH DRGFG+G +NWL+ FE
Sbjct: 124 YVPITIVQNAVAKGAVCLDGSPPAYHFDRGFGSGINNWLVAFE 252
>TC205391 similar to UP|Q8L7Z5 (Q8L7Z5) At1g57590/T8L23_6, partial (82%)
Length = 1469
Score = 63.2 bits (152), Expect = 2e-11
Identities = 26/50 (52%), Positives = 35/50 (70%)
Frame = +1
Query: 31 SQEPKKLYVNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
S + L V++TL+ A GA CLDG++P YHLDRGFG+G D+WL+ E
Sbjct: 175 SSSSQPLMVDLTLIQGADSKGAVCLDGTVPGYHLDRGFGSGADSWLIHLE 324
>BM307860
Length = 418
Score = 60.8 bits (146), Expect = 1e-10
Identities = 30/69 (43%), Positives = 39/69 (56%)
Frame = +2
Query: 12 FAVAFALFYLITVESWRVHSQEPKKLYVNMTLVNNARETGAFCLDGSLPAYHLDRGFGAG 71
F + F+L ++S R SQ V +TL+ NA +T A CLDGS P YH GFG+G
Sbjct: 80 FLILFSLLLFSHLDS-RYDSQPHHSKLVPLTLLRNANQTRALCLDGSAPGYHFQSGFGSG 256
Query: 72 EDNWLLQFE 80
NWL+ E
Sbjct: 257 SRNWLIHIE 283
>TC205388 similar to UP|Q8L7Z5 (Q8L7Z5) At1g57590/T8L23_6, partial (86%)
Length = 1433
Score = 57.4 bits (137), Expect = 1e-09
Identities = 24/47 (51%), Positives = 31/47 (65%)
Frame = +1
Query: 34 PKKLYVNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
P L V +TL+ A GA CLDG+LP YH GFG+G ++WL+Q E
Sbjct: 160 PPPLMVPLTLIQGAASKGAVCLDGTLPGYHFHPGFGSGANSWLIQLE 300
>TC205392
Length = 517
Score = 57.4 bits (137), Expect = 1e-09
Identities = 24/47 (51%), Positives = 31/47 (65%)
Frame = +3
Query: 34 PKKLYVNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
P L V +TL+ A GA CLDG+LP YH GFG+G ++WL+Q E
Sbjct: 123 PPPLMVPLTLIQGAASKGAVCLDGTLPGYHFHPGFGSGANSWLIQLE 263
>TC204335 homologue to UP|Q41695 (Q41695) Pectinacetylesterase precursor ,
complete
Length = 1724
Score = 54.7 bits (130), Expect = 8e-09
Identities = 24/42 (57%), Positives = 29/42 (68%)
Frame = +3
Query: 39 VNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
V +T V NA GA CLDGS PAYH +G GAG +NW++ FE
Sbjct: 315 VGITFVENAVAKGAVCLDGSPPAYHFHKGSGAGINNWIVHFE 440
>AW598043 similar to PIR|S68805|S688 pectin acetylesterase (EC 3.1.1.-)
precursor - mung bean, partial (23%)
Length = 414
Score = 54.7 bits (130), Expect = 8e-09
Identities = 23/42 (54%), Positives = 29/42 (68%)
Frame = +3
Query: 39 VNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
V + LV NA GA CLDGS PAYH D+GFG G ++W++ E
Sbjct: 123 VPLILVENAESKGAVCLDGSPPAYHFDKGFGEGINSWIVHIE 248
>TC204334 weakly similar to UP|Q41695 (Q41695) Pectinacetylesterase precursor
, partial (95%)
Length = 1508
Score = 54.3 bits (129), Expect = 1e-08
Identities = 24/42 (57%), Positives = 27/42 (64%)
Frame = +1
Query: 39 VNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
V +TLV NA GA CLDGS PAYH D GF G NW++ E
Sbjct: 160 VPLTLVKNAESKGAVCLDGSPPAYHFDNGFEEGIKNWIVHIE 285
>TC204338 weakly similar to UP|Q41695 (Q41695) Pectinacetylesterase precursor
, partial (18%)
Length = 568
Score = 48.1 bits (113), Expect = 7e-07
Identities = 23/40 (57%), Positives = 28/40 (69%)
Frame = +3
Query: 38 YVNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLL 77
YV +T+V NA GA CLDGS PAYH +GFG+G N L+
Sbjct: 399 YVPITIVQNAVAKGAVCLDGSPPAYHFCKGFGSGIINRLV 518
>BE610392
Length = 418
Score = 46.2 bits (108), Expect = 3e-06
Identities = 19/34 (55%), Positives = 25/34 (72%)
Frame = +2
Query: 37 LYVNMTLVNNARETGAFCLDGSLPAYHLDRGFGA 70
L V +TL+ +A GA CLDG+LP YHL RG+G+
Sbjct: 317 LMVGLTLIQSAAAKGAVCLDGTLPGYHLHRGYGS 418
>BF324517 weakly similar to PIR|S68805|S688 pectin acetylesterase (EC
3.1.1.-) precursor - mung bean, partial (11%)
Length = 265
Score = 34.7 bits (78), Expect = 0.008
Identities = 16/31 (51%), Positives = 22/31 (70%)
Frame = +2
Query: 50 TGAFCLDGSLPAYHLDRGFGAGEDNWLLQFE 80
+G+ LDGS PA HLD+ FG+ + WL+ FE
Sbjct: 2 SGSC*LDGSPPAPHLDKRFGSWINYWLVAFE 94
>TC204615 similar to GB|AAL08296.1|15912325|AY056440 At1g60710/F8A5_23
{Arabidopsis thaliana;} , partial (94%)
Length = 2219
Score = 29.3 bits (64), Expect = 0.35
Identities = 13/39 (33%), Positives = 20/39 (50%)
Frame = +2
Query: 50 TGAFCLDGSLPAYHLDRGFGAGEDNWLLQFEVYSYFSHC 88
+G CLD +L + R + G NWL + +V+ HC
Sbjct: 1682 SGRLCLDFNLKIWSRTRQYSQGLMNWLQRRDVHHLSLHC 1798
>BE608416 weakly similar to PIR|T05825|T058 pectin acetylesterase homolog
T5K18.200 - Arabidopsis thaliana, partial (13%)
Length = 385
Score = 28.9 bits (63), Expect = 0.46
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +2
Query: 39 VNMTLVNNARETGAFCLDGSLPAYHLDRGF 68
+ +TL+NN A L+ + PAYH +R F
Sbjct: 77 IPLTLINNTESKRAIYLNKNPPAYHFNRQF 166
>TC230317
Length = 1368
Score = 26.2 bits (56), Expect = 3.0
Identities = 12/31 (38%), Positives = 20/31 (63%)
Frame = -2
Query: 29 VHSQEPKKLYVNMTLVNNARETGAFCLDGSL 59
V ++EPKKL++ + ++ E+G F L G L
Sbjct: 170 VDAKEPKKLHLFLFSIHEGLESGFFFLYGGL 78
>TC228350 similar to UP|Q75IA4 (Q75IA4) Expressed protein, partial (65%)
Length = 1410
Score = 25.8 bits (55), Expect = 3.9
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +1
Query: 9 TMNFAVAFALFYLITVESWRVHSQEPKKLYVNMTL 43
T FAVA A+F++ + + V+ P Y N+ L
Sbjct: 298 TWEFAVAVAVFFVFSTGLFCVYLSLPSAAYTNLKL 402
>TC224608 UP|VATL_PHAAU (O22552) Vacuolar ATP synthase 16 kDa proteolipid
subunit , complete
Length = 886
Score = 25.4 bits (54), Expect = 5.0
Identities = 18/62 (29%), Positives = 25/62 (40%)
Frame = +2
Query: 27 WRVHSQEPKKLYVNMTLVNNARETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFEVYSYFS 86
W S+ + ++ + V N E C+DG A D AG D W + Y F
Sbjct: 92 WLPRSRRCPRFFLYGSGVRNREERRRGCVDGRDEAGAGDEIDRAGCDGWCVG---YLRFD 262
Query: 87 HC 88
HC
Sbjct: 263 HC 268
>TC204618 similar to UP|Q9ASZ9 (Q9ASZ9) At1g60730/F8A5_24, partial (58%)
Length = 828
Score = 25.0 bits (53), Expect = 6.6
Identities = 13/40 (32%), Positives = 20/40 (49%)
Frame = +2
Query: 48 RETGAFCLDGSLPAYHLDRGFGAGEDNWLLQFEVYSYFSH 87
+ TG CLD +L + R + G NWL + +V+ H
Sbjct: 299 KTTGRDCLDFNLKIWSRIRLYSRGLMNWLQKKDVHHLSLH 418
>TC234824 similar to UP|Q9I7K2 (Q9I7K2) CG4655-PA, partial (4%)
Length = 426
Score = 24.6 bits (52), Expect = 8.6
Identities = 9/29 (31%), Positives = 19/29 (65%)
Frame = +1
Query: 18 LFYLITVESWRVHSQEPKKLYVNMTLVNN 46
LF+L+ + SW V + +K ++N+ + +N
Sbjct: 169 LFFLLLIASWGVWK*KEEKKFINIHIHSN 255
>CA784288
Length = 422
Score = 24.6 bits (52), Expect = 8.6
Identities = 14/37 (37%), Positives = 18/37 (47%), Gaps = 3/37 (8%)
Frame = +3
Query: 53 FCLDGSLPAYHLDRGFGAGEDNWLLQFE---VYSYFS 86
F L G L YH G + NW+L F + S+FS
Sbjct: 30 FSLQG*LHHYHRSHTTGVPKPNWVLCFNTNTMLSFFS 140
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.326 0.137 0.436
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,939,666
Number of Sequences: 63676
Number of extensions: 58056
Number of successful extensions: 357
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 356
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 356
length of query: 90
length of database: 12,639,632
effective HSP length: 66
effective length of query: 24
effective length of database: 8,437,016
effective search space: 202488384
effective search space used: 202488384
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)
Medicago: description of AC131240.2