BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC129091.6 - phase: 0 /pseudo
(1077 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC219163 232 8e-61
TC219162 192 9e-49
CO980552 135 2e-46
BI785584 149 5e-36
BU082693 129 9e-30
BE346645 97 5e-20
BE346909 93 7e-19
AI960758 35 3e-05
TC227949 similar to UP|Q9ZV88 (Q9ZV88) F9K20.26 protein (At1g787... 32 1.5
CO985896 32 2.0
BU549955 32 2.0
CD392500 31 3.4
BM887294 31 3.4
TC220803 weakly similar to UP|Q8LAD3 (Q8LAD3) Glu-tRNA(Gln) amid... 31 3.4
BG882257 30 4.4
CO982792 30 4.4
CO984133 30 5.7
TC230312 similar to UP|Q9XI34 (Q9XI34) F9L1.30, partial (5%) 30 7.5
CO982259 30 7.5
>TC219163
Length = 895
Score = 232 bits (591), Expect = 8e-61
Identities = 112/177 (63%), Positives = 128/177 (72%), Gaps = 12/177 (6%)
Frame = +2
Query: 913 KMAMQNIPRPRGVDDTRLLLRKLELLPPAEVWHRLNMFGGPCTDPD------DSPRPVRS 966
KMAMQN+PRPRG D LLL +LEL PPAE WHR NMFGGP +DPD D+ V
Sbjct: 101 KMAMQNMPRPRGADAAGLLLCELELHPPAEEWHRRNMFGGPWSDPDVLDFANDALNLVSL 280
Query: 967 NPLDSRSLESNNIDYGTNGLWPRKHRMIERDAAFALNTS------LDIMGSRRDVITTSW 1020
+PLDS SLE ++ YG NGLW R RM ERDAAF LNT L IMGSRRD +T W
Sbjct: 281 HPLDSSSLEYCDVYYGANGLWSR*RRMSERDAAFGLNTYVGLGAYLGIMGSRRDAVTALW 460
Query: 1021 KTGLEGVWYKCIRCMRQTSAFTSPASANIPNQNERESWWVSRWATNCPMCGGRWVRV 1077
KTG+EG+WYKC+RC+RQT AF SPAS N+P+QN+RE WW+S WA CPMCGG WVRV
Sbjct: 461 KTGIEGIWYKCMRCLRQTCAFASPASTNLPSQNDREIWWISSWAYGCPMCGGTWVRV 631
>TC219162
Length = 700
Score = 192 bits (487), Expect = 9e-49
Identities = 89/134 (66%), Positives = 102/134 (75%), Gaps = 12/134 (8%)
Frame = +3
Query: 956 DPDD------SPRPVRSNPLDSRSLESNNIDYGTNGLWPRKHRMIERDAAFALNTS---- 1005
DPDD +P+ + NPLDS SLE+ ++ YG NGLWPRK RM ERDAAF LNTS
Sbjct: 3 DPDDLDSANDAPKLISLNPLDSSSLENCDVYYGANGLWPRKRRMSERDAAFGLNTSVGLG 182
Query: 1006 --LDIMGSRRDVITTSWKTGLEGVWYKCIRCMRQTSAFTSPASANIPNQNERESWWVSRW 1063
L IMGSRRDV+T WKTGLEG+WYKCIRC+RQT AF SPAS N+P+QN+RE WW+SRW
Sbjct: 183 AYLGIMGSRRDVVTALWKTGLEGIWYKCIRCLRQTCAFASPASTNLPSQNDREIWWISRW 362
Query: 1064 ATNCPMCGGRWVRV 1077
A CPMCGG WVRV
Sbjct: 363 AYGCPMCGGTWVRV 404
>CO980552
Length = 685
Score = 135 bits (340), Expect(2) = 2e-46
Identities = 62/89 (69%), Positives = 68/89 (75%)
Frame = -2
Query: 217 TQNATLHKWFCTSKGLLACGPSGIITGDAIITDNGTLLVAGVPAVNPAAIIVWEVMPGRG 276
TQN T KWFCTSKGLL CGPSGI+ GDAIITD+G + VAGVP VNP+ I+VWEV PG G
Sbjct: 684 TQNGTTPKWFCTSKGLLGCGPSGIMAGDAIITDSGAMHVAGVPIVNPSTIVVWEVAPGPG 505
Query: 277 NSLQFSPKKSINNGVPPLSAPNWSGFAPL 305
N Q +PK S GVPPLS PNW GFA L
Sbjct: 504 NGFQVTPKTSTTCGVPPLSPPNWDGFAAL 418
Score = 70.5 bits (171), Expect(2) = 2e-46
Identities = 48/74 (64%), Positives = 52/74 (69%), Gaps = 1/74 (1%)
Frame = -3
Query: 305 LYHFTARQFQNFQHM*VLSLQLNLQHPLPGVLV*QGQPSIRLVTVL**LL**LRVCI*F* 364
LY + A QF+ FQHM*VL LQLNLQ PL GVLV*Q P I+LV V **LL**LRV *F*
Sbjct: 332 LYLYIAHQFRIFQHM*VLKLQLNLQQPLHGVLV*QQWPLIQLVRVQ**LL**LRV*S*F* 153
Query: 365 LDL-PVLQFQYILC 377
L P + FQ C
Sbjct: 152 LFFSPQISFQIAQC 111
>BI785584
Length = 421
Score = 149 bits (375), Expect(2) = 5e-36
Identities = 77/123 (62%), Positives = 89/123 (71%), Gaps = 12/123 (9%)
Frame = +1
Query: 916 MQNIPRPRGVDDTRLLLRKLELLPPAEVWHRLNMFGGPCTDPD------DSPRPVRSNPL 969
MQN+PRPRG D LLLR+LEL PPAE WHR NMFG P +DP+ D+P+ V S+PL
Sbjct: 52 MQNMPRPRGADAAGLLLRELELHPPAEEWHRRNMFGAPWSDPEDVDCVNDTPKLVNSDPL 231
Query: 970 DSRSLESNNIDYGTNGLWPRKHRMIERDAAFALNTS------LDIMGSRRDVITTSWKTG 1023
D SLE ++ YGT+ LWPRK RM ERDAAF LNTS L IMGSRRDV+T +WKTG
Sbjct: 232 DFSSLEHCDVYYGTHRLWPRKRRMSERDAAFGLNTSVGLGGYLGIMGSRRDVVTATWKTG 411
Query: 1024 LEG 1026
LEG
Sbjct: 412 LEG 420
Score = 21.9 bits (45), Expect(2) = 5e-36
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = +3
Query: 889 ELHCTS*GFGSIPCHTSA 906
EL CT+ G GSIP T A
Sbjct: 3 EL*CTARGCGSIPWSTYA 56
>BU082693
Length = 429
Score = 129 bits (323), Expect = 9e-30
Identities = 66/89 (74%), Positives = 72/89 (80%), Gaps = 1/89 (1%)
Frame = +2
Query: 16 PMIEE-VEAGTVFHIRLKQSPSTLLHKMNLHESMRRNFSAVAWCAKLNAIACATETCARI 74
PM E+ V TVF IRLKQ S LLHKM++ E + RNFSAV+WC KLNAIACA ETCARI
Sbjct: 164 PMEEDPVNPATVFSIRLKQPRSNLLHKMSVPE-LCRNFSAVSWCGKLNAIACAAETCARI 340
Query: 75 PRSSVNPPFWIPIHIVIPERPTENAVFNV 103
P S+ NPPFWIPIHIVIPERPTE AVFNV
Sbjct: 341 PSSTANPPFWIPIHIVIPERPTECAVFNV 427
>BE346645
Length = 187
Score = 96.7 bits (239), Expect = 5e-20
Identities = 37/53 (69%), Positives = 43/53 (80%)
Frame = +3
Query: 1025 EGVWYKCIRCMRQTSAFTSPASANIPNQNERESWWVSRWATNCPMCGGRWVRV 1077
EGVWYKC+RC RQTSAFTSP + P+QN+RE WW+SRW +CPMCGG W RV
Sbjct: 3 EGVWYKCVRCQRQTSAFTSPDATASPSQNDREMWWISRWVHSCPMCGGTWARV 161
>BE346909
Length = 435
Score = 92.8 bits (229), Expect = 7e-19
Identities = 51/106 (48%), Positives = 64/106 (60%), Gaps = 23/106 (21%)
Frame = +3
Query: 800 GDCHFLQRLCQLLFFCFFFKRSQLVLYMNGLRRTAETSL--------------------- 838
GDCHFL RLCQLL FCFFF+R+QL Y+N RT++T++
Sbjct: 3 GDCHFLHRLCQLLLFCFFFRRTQLPRYIN---RTSDTNIQKPQSNTPAPGKVEEIAKPVS 173
Query: 839 --VRSDDGQTGRAGQIVHGSKGGEEPSLGHIRLGTGNSGQGYSFKE 882
V+SDDGQTGR G+KG EE G RLG+GN+GQGY+F+E
Sbjct: 174 AVVKSDDGQTGRT-----GAKGAEEVPSGRSRLGSGNAGQGYTFEE 296
>AI960758
Length = 204
Score = 35.4 bits (80), Expect(2) = 3e-05
Identities = 16/22 (72%), Positives = 18/22 (81%)
Frame = +2
Query: 916 MQNIPRPRGVDDTRLLLRKLEL 937
MQN+PRPRG D LLLR+LEL
Sbjct: 137 MQNMPRPRGADAAGLLLRELEL 202
Score = 31.6 bits (70), Expect(2) = 3e-05
Identities = 15/19 (78%), Positives = 16/19 (83%)
Frame = +1
Query: 883 AALY*GELHCTS*GFGSIP 901
AALY* ELHCT+*G GS P
Sbjct: 70 AALY*WELHCTA*GCGSSP 126
>TC227949 similar to UP|Q9ZV88 (Q9ZV88) F9K20.26 protein
(At1g78700/F9K20_26), partial (78%)
Length = 1834
Score = 32.0 bits (71), Expect = 1.5
Identities = 24/68 (35%), Positives = 28/68 (40%), Gaps = 8/68 (11%)
Frame = +2
Query: 804 FLQRLCQLLFF--------CFFFKRSQLVLYMNGLRRTAETSLVRSDDGQTGRAGQIVHG 855
F C L FF CFFF VL+ L A S Q GR+G+ G
Sbjct: 212 FAYSRCVLCFFLTFVFACVCFFF--CSFVLFGASLIFPARRG*RPSRGSQRGRSGRTTRG 385
Query: 856 SKGGEEPS 863
+GG EPS
Sbjct: 386 ERGGGEPS 409
>CO985896
Length = 851
Score = 31.6 bits (70), Expect = 2.0
Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 6/50 (12%)
Frame = +3
Query: 50 RNFSAVAWCAKLNAIACATET-----CARIPRSSV-NPPFWIPIHIVIPE 93
R F + WC L + E C +P S V +PPF + IHI++P+
Sbjct: 639 RKFHKIYWCCCLTVLNQKKELQISYKCFWVPASVVLDPPFKVSIHIILPK 788
>BU549955
Length = 627
Score = 31.6 bits (70), Expect = 2.0
Identities = 18/76 (23%), Positives = 38/76 (49%)
Frame = -1
Query: 420 MYSGLFVTVVNFNDMLHILFDGEFLKQFSATTFLNTVLSAWFGVFNFCYTYLYLYSHW*N 479
+YSG + ++N +++ LK F + ++ +L + ++ + Y Y+Y+Y +
Sbjct: 435 IYSGTDIYLINKKKCNNLI-----LKMFLSEIVIDIILKMFLYIYIYIYIYIYIYIY--- 280
Query: 480 NLYCYMYNTPYR*IYL 495
+Y Y+Y Y I L
Sbjct: 279 -IYIYIYIYTYSQIML 235
Score = 30.8 bits (68), Expect = 3.4
Identities = 14/37 (37%), Positives = 21/37 (55%)
Frame = -2
Query: 466 FCYTYLYLYSHW*NNLYCYMYNTPYR*IYLPTY*MCF 502
+ Y Y+Y+Y +Y Y+Y Y IY+PT +CF
Sbjct: 326 YIYIYIYIY------IYIYIYIYIYIYIYIPTVRLCF 234
>CD392500
Length = 326
Score = 30.8 bits (68), Expect = 3.4
Identities = 14/30 (46%), Positives = 16/30 (52%)
Frame = +1
Query: 1044 PASANIPNQNERESWWVSRWATNCPMCGGR 1073
P +AN P ER WW+ R P CGGR
Sbjct: 217 PNAAN-PEVEERSKWWLMRSFEISPTCGGR 303
>BM887294
Length = 421
Score = 30.8 bits (68), Expect = 3.4
Identities = 12/32 (37%), Positives = 18/32 (55%)
Frame = +3
Query: 460 WFGVFNFCYTYLYLYSHW*NNLYCYMYNTPYR 491
+F +FNF + Y +LY H N + Y + YR
Sbjct: 297 FFNIFNFVFIYFFLYQHLKNIKFTGYYKSIYR 392
>TC220803 weakly similar to UP|Q8LAD3 (Q8LAD3) Glu-tRNA(Gln) amidotransferase
subunit C, partial (35%)
Length = 643
Score = 30.8 bits (68), Expect = 3.4
Identities = 14/30 (46%), Positives = 16/30 (52%)
Frame = -2
Query: 1044 PASANIPNQNERESWWVSRWATNCPMCGGR 1073
P +AN P ER WW+ R P CGGR
Sbjct: 168 PNAAN-PEVEERSKWWLMRSFEISPTCGGR 82
>BG882257
Length = 388
Score = 30.4 bits (67), Expect = 4.4
Identities = 15/35 (42%), Positives = 21/35 (59%)
Frame = +3
Query: 461 FGVFNFCYTYLYLYSHW*NNLYCYMYNTPYR*IYL 495
F VFNF Y Y+Y+Y + +Y Y+Y Y IY+
Sbjct: 180 FLVFNFIYIYIYIYIY----IYIYIYIYIYIYIYI 272
>CO982792
Length = 522
Score = 30.4 bits (67), Expect = 4.4
Identities = 14/45 (31%), Positives = 25/45 (55%)
Frame = +1
Query: 454 NTVLSAWFGVFNFCYTYLYLYSHW*NNLYCYMYNTPYR*IYLPTY 498
N V+S + ++ + Y Y+Y+Y + +Y Y+Y Y IY+ Y
Sbjct: 361 NLVISYYIYIYIYIYIYIYIYIYIYIYIYIYIYIYIYIYIYIYIY 495
>CO984133
Length = 240
Score = 30.0 bits (66), Expect = 5.7
Identities = 13/39 (33%), Positives = 23/39 (58%)
Frame = -1
Query: 463 VFNFCYTYLYLYSHW*NNLYCYMYNTPYR*IYLPTY*MC 501
++ + Y Y+Y+Y + +Y Y+Y Y IY+ Y*+C
Sbjct: 213 IYIYIYIYIYIYIYIYIYIYIYIYIYIYIYIYIYIY*IC 97
Score = 29.6 bits (65), Expect = 7.5
Identities = 13/54 (24%), Positives = 26/54 (48%)
Frame = +1
Query: 445 KQFSATTFLNTVLSAWFGVFNFCYTYLYLYSHW*NNLYCYMYNTPYR*IYLPTY 498
K S + ++ + ++ + Y Y+Y+Y + +Y Y+Y Y IY+ Y
Sbjct: 52 KSVSVLAYFFFIIRVTYSIYIYIYIYIYIYIYIYIYIYIYIYIYIYIYIYIYIY 213
>TC230312 similar to UP|Q9XI34 (Q9XI34) F9L1.30, partial (5%)
Length = 701
Score = 29.6 bits (65), Expect = 7.5
Identities = 26/83 (31%), Positives = 33/83 (39%)
Frame = -3
Query: 737 GTTISGGSTQMNAWVQEAIAKISSTSDGVSNLTPTSPIGGPSSLMPISINTETFPGTPAV 796
GT + GG+ M VQ + + GV+ T GG F GTP
Sbjct: 336 GTAVQGGTWFMGTAVQGGTWFMRNVEQGVTWFMRTPVRGGT-----------WFKGTP-- 196
Query: 797 RLIGDCHFLQRLCQLLFFCFFFK 819
+IGD R L FF FFF+
Sbjct: 195 -VIGDSGVCDRNFLLDFFLFFFR 130
>CO982259
Length = 306
Score = 29.6 bits (65), Expect = 7.5
Identities = 13/41 (31%), Positives = 22/41 (52%)
Frame = +3
Query: 458 SAWFGVFNFCYTYLYLYSHW*NNLYCYMYNTPYR*IYLPTY 498
S W ++ + Y Y+Y+Y + +Y Y+Y Y IY+ Y
Sbjct: 174 SNWIYIYIYIYIYIYIYIYIYIYIYIYIYIYIYIYIYIYIY 296
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.340 0.148 0.510
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,542,593
Number of Sequences: 63676
Number of extensions: 947786
Number of successful extensions: 11138
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 9326
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 10207
length of query: 1077
length of database: 12,639,632
effective HSP length: 107
effective length of query: 970
effective length of database: 5,826,300
effective search space: 5651511000
effective search space used: 5651511000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 64 (29.3 bits)
Medicago: description of AC129091.6