BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC129091.12 + phase: 0
(162 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC224043 similar to UP|Q6VY19 (Q6VY19) NADPH HC toxin reductase ... 152 7e-38
TC217112 weakly similar to UP|P93188 (P93188) NADPH-dependent HC... 138 1e-33
TC217111 similar to UP|P93188 (P93188) NADPH-dependent HC-toxin ... 77 4e-25
TC225589 similar to UP|O04391 (O04391) Cinnamyl alcohol dehydrog... 89 9e-19
TC208377 similar to GB|AAM64538.1|21592589|AY086975 cinnamoyl-Co... 88 2e-18
TC226859 homologue to UP|O48602 (O48602) 2'-hydroxydihydrodaidze... 87 5e-18
TC213000 similar to UP|Q40316 (Q40316) Vestitone reductase, part... 87 5e-18
CF808387 87 5e-18
TC210161 UP|Q9SDZ2 (Q9SDZ2) 2'-hydroxy isoflavone/dihydroflavono... 86 8e-18
TC215695 similar to UP|P93700 (P93700) CPRD14 protein , complete 86 8e-18
TC224892 similar to UP|O48602 (O48602) 2'-hydroxydihydrodaidzein... 85 2e-17
TC217827 80 3e-16
TC217923 UP|Q9SPJ5 (Q9SPJ5) Dihydroflavonol-4-reductase DFR1, co... 80 4e-16
TC218842 similar to UP|Q9SPJ5 (Q9SPJ5) Dihydroflavonol-4-reducta... 80 6e-16
TC225292 weakly similar to GB|AAM64538.1|21592589|AY086975 cinna... 77 3e-15
TC225590 weakly similar to UP|O04391 (O04391) Cinnamyl alcohol d... 77 3e-15
TC225293 weakly similar to GB|AAM64538.1|21592589|AY086975 cinna... 77 5e-15
TC226045 similar to UP|Q9FSC6 (Q9FSC6) Cinnamoyl-CoA reductase ... 76 8e-15
TC226046 similar to UP|Q7Y0H8 (Q7Y0H8) Cinnamoyl CoA reductase, ... 76 8e-15
TC225591 similar to UP|O04391 (O04391) Cinnamyl alcohol dehydrog... 75 2e-14
>TC224043 similar to UP|Q6VY19 (Q6VY19) NADPH HC toxin reductase (Fragment),
partial (32%)
Length = 575
Score = 152 bits (384), Expect = 7e-38
Identities = 73/91 (80%), Positives = 79/91 (86%), Gaps = 1/91 (1%)
Frame = +1
Query: 4 RCKVCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHAN-TRLVLFEA 62
RCKVCVTGGA YIGS LVKKLL+KGYTVH+TLRN KDESK+ LRG PHAN RLVLFEA
Sbjct: 16 RCKVCVTGGASYIGSCLVKKLLQKGYTVHSTLRNFKDESKIGLLRGLPHANDERLVLFEA 195
Query: 63 DIYKPDDFGTAIQGCEFVFHVATPYLHQTDS 93
DIYKPD++ AIQGCE VFHVATPY HQ+DS
Sbjct: 196 DIYKPDEYEPAIQGCEIVFHVATPYEHQSDS 288
>TC217112 weakly similar to UP|P93188 (P93188) NADPH-dependent HC-toxin
reductase , partial (33%)
Length = 1054
Score = 138 bits (347), Expect = 1e-33
Identities = 64/93 (68%), Positives = 76/93 (80%)
Frame = +1
Query: 2 ERRCKVCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFE 61
E CKVCVTGG+GYIGS L+KKLL KGYTVHATLR+LK+ESKV L+ P + +LVLFE
Sbjct: 46 EEGCKVCVTGGSGYIGSWLIKKLLAKGYTVHATLRDLKNESKVGLLKSLPQSEGKLVLFE 225
Query: 62 ADIYKPDDFGTAIQGCEFVFHVATPYLHQTDSQ 94
ADIY P+DF AI+GC+FVFHVATP +H+ SQ
Sbjct: 226 ADIYNPNDFDLAIEGCKFVFHVATPMIHEPGSQ 324
>TC217111 similar to UP|P93188 (P93188) NADPH-dependent HC-toxin reductase ,
partial (23%)
Length = 1312
Score = 77.4 bits (189), Expect(2) = 4e-25
Identities = 35/56 (62%), Positives = 43/56 (76%)
Frame = +1
Query: 39 KDESKVSFLRGFPHANTRLVLFEADIYKPDDFGTAIQGCEFVFHVATPYLHQTDSQ 94
++ESKVS L+ P + +LVLFEADIY P+DF AI+GCEFVFHVATP +H SQ
Sbjct: 328 ENESKVSLLKSLPQSEGKLVLFEADIYNPNDFDHAIEGCEFVFHVATPMIHDPGSQ 495
Score = 53.5 bits (127), Expect(2) = 4e-25
Identities = 25/33 (75%), Positives = 29/33 (87%)
Frame = +2
Query: 6 KVCVTGGAGYIGSLLVKKLLEKGYTVHATLRNL 38
KVCVTG +GYI S LVKKLL KG++VHATLR+L
Sbjct: 146 KVCVTGASGYIASSLVKKLLAKGHSVHATLRDL 244
>TC225589 similar to UP|O04391 (O04391) Cinnamyl alcohol dehydrogenase ,
partial (97%)
Length = 1242
Score = 89.0 bits (219), Expect = 9e-19
Identities = 54/131 (41%), Positives = 76/131 (57%)
Frame = +3
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG AGYI S +VK LLE+GYTV AT+RN D +KV L A RL LF+AD+
Sbjct: 42 VCVTGAAGYIASWIVKFLLERGYTVRATVRNPNDHTKVEHLLKLEGAKERLHLFKADLLG 221
Query: 67 PDDFGTAIQGCEFVFHVATPYLHQTDSQVNIQILIISLFFTHGNGAMWGRVLPSPSPSPT 126
+ F + ++GC+ VFH A+P++ +N++ L G + VL S SP+
Sbjct: 222 ENSFDSIVEGCDGVFHTASPFI------INVKDPQADLLDPAVKGTL--NVLKSCVKSPS 377
Query: 127 LIYLPLTLSIA 137
+ + LT S+A
Sbjct: 378 VKRVILTSSVA 410
>TC208377 similar to GB|AAM64538.1|21592589|AY086975 cinnamoyl-CoA
reductase-like protein {Arabidopsis thaliana;} ,
partial (98%)
Length = 1087
Score = 88.2 bits (217), Expect = 2e-18
Identities = 42/80 (52%), Positives = 58/80 (72%)
Frame = +2
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTGG+G IGS LV LL++GYTVHAT++NL DE++ L+ A+TRL LF+ D+ +
Sbjct: 17 VCVTGGSGCIGSWLVHLLLDRGYTVHATVQNLNDEAETKHLQSLDGASTRLRLFQMDLLR 196
Query: 67 PDDFGTAIQGCEFVFHVATP 86
D A++GC VFH+A+P
Sbjct: 197HDTVLAAVRGCAGVFHLASP 256
>TC226859 homologue to UP|O48602 (O48602) 2'-hydroxydihydrodaidzein
reductase, complete
Length = 1364
Score = 86.7 bits (213), Expect = 5e-18
Identities = 39/87 (44%), Positives = 59/87 (66%), Gaps = 1/87 (1%)
Frame = +2
Query: 1 MERRCKVCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESK-VSFLRGFPHANTRLVL 59
+E + ++CVTGG G++GS ++K LLE GY V+ T+R+ + VSFL P A+ +L +
Sbjct: 62 VEGKGRICVTGGTGFLGSWIIKSLLEHGYAVNTTIRSDPGRKRDVSFLTNLPGASEKLKI 241
Query: 60 FEADIYKPDDFGTAIQGCEFVFHVATP 86
F AD+ P+ FG A++GC +FH ATP
Sbjct: 242 FNADLSDPESFGPAVEGCVGIFHTATP 322
>TC213000 similar to UP|Q40316 (Q40316) Vestitone reductase, partial (52%)
Length = 525
Score = 86.7 bits (213), Expect = 5e-18
Identities = 42/86 (48%), Positives = 58/86 (66%), Gaps = 1/86 (1%)
Frame = +1
Query: 2 ERRCKVCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESK-VSFLRGFPHANTRLVLF 60
E + +VCVTGG G+IGS ++K+LLE GYTV+ T+R+ + VSFL P A+ +L +F
Sbjct: 28 EEKGRVCVTGGTGFIGSWIIKRLLEGGYTVNTTIRSDPGRKRDVSFLTNLPFASKKLRIF 207
Query: 61 EADIYKPDDFGTAIQGCEFVFHVATP 86
AD+ P+ F AI+GC V H ATP
Sbjct: 208 NADLSNPESFSEAIEGCIGVLHTATP 285
>CF808387
Length = 686
Score = 86.7 bits (213), Expect = 5e-18
Identities = 39/87 (44%), Positives = 59/87 (66%), Gaps = 1/87 (1%)
Frame = +1
Query: 1 MERRCKVCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESK-VSFLRGFPHANTRLVL 59
+E + ++CVTGG G++GS ++K LLE GY V+ T+R+ + VSFL P A+ +L +
Sbjct: 406 VEGKGRICVTGGTGFLGSWIIKSLLEHGYAVNTTIRSDPGRKRDVSFLTNLPGASEKLKI 585
Query: 60 FEADIYKPDDFGTAIQGCEFVFHVATP 86
F AD+ P+ FG A++GC +FH ATP
Sbjct: 586 FNADLSDPESFGPAVEGCVGIFHTATP 666
>TC210161 UP|Q9SDZ2 (Q9SDZ2) 2'-hydroxy isoflavone/dihydroflavonol reductase
homolog (Fragment), complete
Length = 1336
Score = 85.9 bits (211), Expect = 8e-18
Identities = 42/82 (51%), Positives = 57/82 (69%), Gaps = 1/82 (1%)
Frame = +1
Query: 6 KVCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESK-VSFLRGFPHANTRLVLFEADI 64
+VCVTGG G+IGS ++K+LLE GY+V+ T+R + K VSFL P A+ RL + AD+
Sbjct: 22 RVCVTGGTGFIGSWIIKRLLEDGYSVNTTVRPDPEHRKDVSFLTSLPRASQRLQILSADL 201
Query: 65 YKPDDFGTAIQGCEFVFHVATP 86
P+ F +I+GC VFHVATP
Sbjct: 202 SNPESFIASIEGCMGVFHVATP 267
>TC215695 similar to UP|P93700 (P93700) CPRD14 protein , complete
Length = 1414
Score = 85.9 bits (211), Expect = 8e-18
Identities = 52/131 (39%), Positives = 73/131 (55%)
Frame = +1
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG +GYI S +VK LL +GYTV AT+R+ D K++ L G A RL L++A++ +
Sbjct: 202 VCVTGASGYIASWIVKFLLNRGYTVKATVRDTSDPKKINHLVGLDGAKERLHLYKANLLE 381
Query: 67 PDDFGTAIQGCEFVFHVATPYLHQTDSQVNIQILIISLFFTHGNGAMWGRVLPSPSPSPT 126
F + +QGC VFH A+P+ H N++ L G + VL S PT
Sbjct: 382 EGSFNSVVQGCHAVFHTASPFYH------NVKDPQAELLDPALKGTL--NVLKSCVNLPT 537
Query: 127 LIYLPLTLSIA 137
L + LT S+A
Sbjct: 538 LERVVLTSSVA 570
>TC224892 similar to UP|O48602 (O48602) 2'-hydroxydihydrodaidzein reductase,
complete
Length = 1368
Score = 84.7 bits (208), Expect = 2e-17
Identities = 39/86 (45%), Positives = 58/86 (67%), Gaps = 1/86 (1%)
Frame = +2
Query: 2 ERRCKVCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESK-VSFLRGFPHANTRLVLF 60
E + +VCVTGG G++GS ++K+LLE GY V+ T+R+ + VSFL P A+ +L +F
Sbjct: 119 EGKGRVCVTGGTGFLGSWIIKRLLEDGYAVNTTIRSDPGRKRDVSFLTNLPGASEKLKIF 298
Query: 61 EADIYKPDDFGTAIQGCEFVFHVATP 86
AD+ P+ F A++GC +FH ATP
Sbjct: 299 NADLSDPESFDPAVEGCVGIFHTATP 376
>TC217827
Length = 1189
Score = 80.5 bits (197), Expect = 3e-16
Identities = 42/93 (45%), Positives = 55/93 (58%)
Frame = +1
Query: 8 CVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYKP 67
CVTGG G+I S LVK LLEKG+TV T+RN D KV FL A RL + +AD+
Sbjct: 49 CVTGGTGFIASYLVKALLEKGHTVRTTVRNPGDVEKVGFLTELSGAKERLKILKADLLVE 228
Query: 68 DDFGTAIQGCEFVFHVATPYLHQTDSQVNIQIL 100
F A++G + VFH+A+P L D V ++
Sbjct: 229 GSFDEAVRGVDGVFHMASPVLIPYDENVQQNLI 327
>TC217923 UP|Q9SPJ5 (Q9SPJ5) Dihydroflavonol-4-reductase DFR1, complete
Length = 1254
Score = 80.1 bits (196), Expect = 4e-16
Identities = 40/80 (50%), Positives = 56/80 (70%)
Frame = +3
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG +G+IGS LV +L+++GYTV AT+R+ + KV L P A ++L L++AD+ +
Sbjct: 72 VCVTGASGFIGSWLVMRLIKRGYTVRATVRDPVNMKKVKNLVELPGAKSKLSLWKADLAE 251
Query: 67 PDDFGTAIQGCEFVFHVATP 86
F AI+GC VFHVATP
Sbjct: 252 EGSFDEAIKGCTGVFHVATP 311
>TC218842 similar to UP|Q9SPJ5 (Q9SPJ5) Dihydroflavonol-4-reductase DFR1,
partial (42%)
Length = 496
Score = 79.7 bits (195), Expect = 6e-16
Identities = 39/80 (48%), Positives = 55/80 (68%)
Frame = +2
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG +GYIGS LV +L+E+GYTV AT+ + D +V L P A ++L L++A++ +
Sbjct: 80 VCVTGASGYIGSWLVMRLIERGYTVRATVLDPADMREVKHLLDLPGAESKLSLWKAELTE 259
Query: 67 PDDFGTAIQGCEFVFHVATP 86
F AI+GC VFH+ATP
Sbjct: 260 EGSFDEAIKGCTGVFHLATP 319
>TC225292 weakly similar to GB|AAM64538.1|21592589|AY086975 cinnamoyl-CoA
reductase-like protein {Arabidopsis thaliana;} , partial
(87%)
Length = 1195
Score = 77.4 bits (189), Expect = 3e-15
Identities = 40/93 (43%), Positives = 56/93 (60%), Gaps = 2/93 (2%)
Frame = +3
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG +G IGS + LL++GYTVHAT++++KDE++ L A +RL FE D+
Sbjct: 60 VCVTGASGAIGSWVALLLLQRGYTVHATVQDIKDENETKHLEEMEGAKSRLHFFEMDLLD 239
Query: 67 PDDFGTAIQGCEFVFHVATPYL--HQTDSQVNI 97
D AI+GC V H+A P + H D + I
Sbjct: 240 IDSIAAAIKGCSGVIHLACPNIIGHVEDPEKQI 338
>TC225590 weakly similar to UP|O04391 (O04391) Cinnamyl alcohol dehydrogenase
, partial (98%)
Length = 1141
Score = 77.4 bits (189), Expect = 3e-15
Identities = 48/131 (36%), Positives = 76/131 (57%)
Frame = +3
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG +G+I S ++K LL++GYTV AT+R+ KV L A RL LF+AD+ +
Sbjct: 75 VCVTGASGFIASWIIKLLLQRGYTVRATVRDPSKPEKVDHLLKLDGAKERLHLFKADLLE 254
Query: 67 PDDFGTAIQGCEFVFHVATPYLHQTDSQVNIQILIISLFFTHGNGAMWGRVLPSPSPSPT 126
F +A +GC+ VFH A+P +H + Q++ ++ G + V+ S + SP+
Sbjct: 255 EGSFDSAFEGCDGVFHTASP-VHFIVTDPQNQLIDPAI-----KGTL--NVVKSCAKSPS 410
Query: 127 LIYLPLTLSIA 137
+ + LT S+A
Sbjct: 411 VKQVILTSSVA 443
>TC225293 weakly similar to GB|AAM64538.1|21592589|AY086975 cinnamoyl-CoA
reductase-like protein {Arabidopsis thaliana;} , partial
(97%)
Length = 1421
Score = 76.6 bits (187), Expect = 5e-15
Identities = 37/80 (46%), Positives = 51/80 (63%)
Frame = +1
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG +G IGS +V LL++GYTVHAT++++KDE++ L A + L FE D+
Sbjct: 142 VCVTGASGAIGSWVVLLLLQRGYTVHATVQDIKDENETKHLEEMEGAKSHLHFFEMDLLD 321
Query: 67 PDDFGTAIQGCEFVFHVATP 86
D AI+GC V H+A P
Sbjct: 322 IDSIAAAIKGCSGVIHLACP 381
>TC226045 similar to UP|Q9FSC6 (Q9FSC6) Cinnamoyl-CoA reductase , partial
(93%)
Length = 1254
Score = 75.9 bits (185), Expect = 8e-15
Identities = 40/89 (44%), Positives = 51/89 (56%)
Frame = +2
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
+CVTG G+I S +VK LLEKGYTV TLRN D+ K L+ F A+ RL L + D+
Sbjct: 80 ICVTGAGGFIASWMVKLLLEKGYTVRGTLRN-PDDPKNGHLKEFEGASERLTLHKVDLLH 256
Query: 67 PDDFGTAIQGCEFVFHVATPYLHQTDSQV 95
D + I GC VFH A+P + V
Sbjct: 257 LDSVRSVINGCHGVFHTASPVTDNPEEMV 343
>TC226046 similar to UP|Q7Y0H8 (Q7Y0H8) Cinnamoyl CoA reductase, partial
(58%)
Length = 780
Score = 75.9 bits (185), Expect = 8e-15
Identities = 40/89 (44%), Positives = 51/89 (56%)
Frame = +1
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
+CVTG G+I S +VK LLEKGYTV TLRN D+ K L+ F A+ RL L + D+
Sbjct: 115 ICVTGAGGFIASWMVKLLLEKGYTVRGTLRN-PDDPKNGHLKEFEGASQRLTLHKVDLLH 291
Query: 67 PDDFGTAIQGCEFVFHVATPYLHQTDSQV 95
D + I GC VFH A+P + V
Sbjct: 292 LDSVRSVINGCHGVFHTASPVTDNPEEMV 378
>TC225591 similar to UP|O04391 (O04391) Cinnamyl alcohol dehydrogenase ,
partial (62%)
Length = 713
Score = 74.7 bits (182), Expect = 2e-14
Identities = 37/80 (46%), Positives = 51/80 (63%)
Frame = +1
Query: 7 VCVTGGAGYIGSLLVKKLLEKGYTVHATLRNLKDESKVSFLRGFPHANTRLVLFEADIYK 66
VCVTG +G+I S +VK LL++GYTV AT+R + KV L A RL LF+AD+ +
Sbjct: 100 VCVTGASGFIASWIVKFLLQRGYTVRATVRYPSNLKKVDHLVKLEGAKERLQLFKADLLE 279
Query: 67 PDDFGTAIQGCEFVFHVATP 86
F + ++GC VFH A+P
Sbjct: 280 EGSFDSVVEGCHGVFHTASP 339
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.323 0.142 0.436
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,571,035
Number of Sequences: 63676
Number of extensions: 138450
Number of successful extensions: 1018
Number of sequences better than 10.0: 120
Number of HSP's better than 10.0 without gapping: 961
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1000
length of query: 162
length of database: 12,639,632
effective HSP length: 90
effective length of query: 72
effective length of database: 6,908,792
effective search space: 497433024
effective search space used: 497433024
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC129091.12