BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC127429.13 - phase: 0 /pseudo
(1603 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW472271 123 6e-28
AW472275 97 4e-20
TC212253 homologue to UP|Q9FNQ1 (Q9FNQ1) RNA helicase, partial (... 59 2e-08
BU764286 53 9e-07
TC205570 homologue to UP|Q93VJ8 (Q93VJ8) AT5g11200/F2I11_90 (AT5... 38 0.041
TC205571 homologue to UP|Q93VJ8 (Q93VJ8) AT5g11200/F2I11_90 (AT5... 37 0.091
AW757077 37 0.091
TC232022 homologue to UP|Q9LU46 (Q9LU46) DEAD-box protein abstra... 34 0.59
TC206536 similar to UP|Q9FZ92 (Q9FZ92) F3H9.16 protein, partial ... 33 1.0
TC234146 homologue to UP|Q84ND9 (Q84ND9) PolI-like DNA polymeras... 33 1.3
BE021424 homologue to GP|11994141|d contains similarity to DNA p... 33 1.3
BI469188 32 1.7
CA784665 32 2.2
TC208786 similar to UP|Q9SMX1 (Q9SMX1) Mitochondrial RNA helicas... 31 3.8
TC210165 similar to UP|Q84ND9 (Q84ND9) PolI-like DNA polymerase,... 31 5.0
TC223634 31 5.0
TC209018 30 6.5
TC210164 similar to UP|Q84ND9 (Q84ND9) PolI-like DNA polymerase,... 30 6.5
TC225745 malate dehydrogenase 30 8.5
CO981930 30 8.5
>AW472271
Length = 259
Score = 123 bits (309), Expect = 6e-28
Identities = 65/83 (78%), Positives = 71/83 (85%)
Frame = +2
Query: 1445 STSQGSAADIIKIAMIKIYSEIFPGVDKLDSSSSVTAKFEMLRDRCRILLQVHDELVLEV 1504
S QGSAADIIKIAM++IYS I G D DSSSS+ KF ML+DRCRILLQVHDEL+LE
Sbjct: 11 SICQGSAADIIKIAMLRIYSAIVTGFDSPDSSSSLPTKFHMLKDRCRILLQVHDELMLEG 190
Query: 1505 DPSVVKEAALLLQTSMENAVSLL 1527
DPSV+KEAALLLQTSMENAVSLL
Sbjct: 191 DPSVIKEAALLLQTSMENAVSLL 259
>AW472275
Length = 259
Score = 97.4 bits (241), Expect = 4e-20
Identities = 54/83 (65%), Positives = 62/83 (74%)
Frame = +2
Query: 1445 STSQGSAADIIKIAMIKIYSEIFPGVDKLDSSSSVTAKFEMLRDRCRILLQVHDELVLEV 1504
S QGSAA II IAM+ I+S I G D SSSS++ K ML+DR RILLQ HDEL++E
Sbjct: 11 SICQGSAAHIINIAMLTIHSAIVTGFDTPHSSSSLSTKSHMLKDRSRILLQGHDELMMEG 190
Query: 1505 DPSVVKEAALLLQTSMENAVSLL 1527
DPS +KEAALL TSMENAVSLL
Sbjct: 191 DPSAIKEAALLWHTSMENAVSLL 259
>TC212253 homologue to UP|Q9FNQ1 (Q9FNQ1) RNA helicase, partial (11%)
Length = 703
Score = 58.5 bits (140), Expect = 2e-08
Identities = 56/239 (23%), Positives = 103/239 (42%), Gaps = 7/239 (2%)
Frame = +1
Query: 3 ILVLPYVSICTEKAEHLEK-LLEPLGKHVRSYYGNQGGG-SLPKDTSVAVCTIEKANSLI 60
I + P +I E+ +K L+ LGK + G+ + ++ + T EK + +
Sbjct: 52 IYIAPLKAIVRERMSDWQKRLVSQLGKKMVEMTGDYTPDLTALLSANIIISTPEKWDGIS 231
Query: 61 NRLLEEGRLSEMGIIVIDELHMVGDPKRGYLLELMLTKLRYAAGEGISKSSDGEGSGGSS 120
++++G++++DE+H++G RG +LE++++++RY + +
Sbjct: 232 RNWHSRSYVTKVGLMILDEIHLLG-ADRGPILEVIVSRMRYISSQ--------------- 363
Query: 121 DKNDPAQGLQIVGMSATMPNVAAVADWLQAAL-----YQTEFRPVPLEEYIKVGNSIYNK 175
+ ++ VG+S + N +ADWL ++ RPVPLE YI+ Y
Sbjct: 364 ----TERAVRFVGLSTALANAGDLADWLGVEEIGLFNFKPSVRPVPLEVYIQGYPGKYYC 531
Query: 176 SMELSRTIPKGADLGGKDPDHVVELCNEVVQEGQSVLIFCSSRKGCESTARHVAKFLKS 234
S P A + P + VLIF SSR+ TA + +F S
Sbjct: 532 PRMNSMNKPAYAAICTHSP-------------AKPVLIFVSSRRQTRLTALDLIQFAAS 669
>BU764286
Length = 428
Score = 53.1 bits (126), Expect = 9e-07
Identities = 31/83 (37%), Positives = 46/83 (55%), Gaps = 10/83 (12%)
Frame = +1
Query: 285 EEREIVETCYRKGLLRVLTATSTLAAGVNLPARRVIFRQPRIGCDFIDG----------T 334
++R +VE + +++L TSTLA GVNLPA VI + G ++ DG T
Sbjct: 7 KDRSLVEELFANNKIQILVCTSTLAWGVNLPAHLVIIK----GTEYYDGKAKRYVDFPIT 174
Query: 335 RYMQMAGRAGRTGIDTKGESVLI 357
+QM GRAGR D G++V++
Sbjct: 175 DILQMMGRAGRPQFDQHGKAVIL 243
>TC205570 homologue to UP|Q93VJ8 (Q93VJ8) AT5g11200/F2I11_90
(AT5g11170/F2I11_60), partial (98%)
Length = 1745
Score = 37.7 bits (86), Expect = 0.041
Identities = 38/161 (23%), Positives = 74/161 (45%), Gaps = 5/161 (3%)
Frame = +1
Query: 200 LCNEVVQEGQSVLIFCSSRKGCESTARHVAKFL---KSFTVDINENNCEFADITSAINSL 256
+C + +Q+ + + ++ +H K K+ ++ + +F + + S+
Sbjct: 835 VCKKFMQDPMEIYVDDEAKLTLHGLVQHYIKLKEEEKNRKLNDLLDALDFNQVVIFVKSV 1014
Query: 257 RKCPAGLDPVLEE-TFPAGVAFHHAGLTVEEREIVETCYRKGLLRVLTATSTLAAGVNLP 315
+ A LD +L E FP+ H+G++ EER +++G R+L AT + G+++
Sbjct: 1015SRA-AELDKLLVECNFPSICI--HSGMSQEERLKRYKGFKEGHTRILVATDLVGRGIDIE 1185
Query: 316 ARRVIFRQPRIGCDFIDGT-RYMQMAGRAGRTGIDTKGESV 355
++ I D D Y+ GRAGR G TKG ++
Sbjct: 1186RVNIV-----INYDMPDSADTYLHRVGRAGRFG--TKGLAI 1287
>TC205571 homologue to UP|Q93VJ8 (Q93VJ8) AT5g11200/F2I11_90
(AT5g11170/F2I11_60), partial (42%)
Length = 1287
Score = 36.6 bits (83), Expect = 0.091
Identities = 31/97 (31%), Positives = 49/97 (49%), Gaps = 2/97 (2%)
Frame = +3
Query: 261 AGLDPVLEE-TFPAGVAFHHAGLTVEEREIVETCYRKGLLRVLTATSTLAAGVNLPARRV 319
A LD +L E FP+ H+G++ EER +++G R+L AT + G+++ +
Sbjct: 603 AELDKLLVECNFPSICI--HSGMSQEERLKRYKGFKEGHTRILVATDLVGRGIDIERVNI 776
Query: 320 IFRQPRIGCDFIDGT-RYMQMAGRAGRTGIDTKGESV 355
+ I D D Y+ GRAGR G TKG ++
Sbjct: 777 V-----INYDMPDSADTYLHRVGRAGRFG--TKGLAI 866
>AW757077
Length = 423
Score = 36.6 bits (83), Expect = 0.091
Identities = 28/98 (28%), Positives = 41/98 (41%), Gaps = 6/98 (6%)
Frame = +1
Query: 75 IVIDELHMVGDPKRGYLLELMLTKLRYAAGEGISKSSDGEGSGGSSDKNDPAQGLQIVGM 134
IV DE+H + D +RG + E + S KN + V +
Sbjct: 4 IVFDEVHYMRDRERGVVWEESIVL---------------------SPKNS-----RFVFL 105
Query: 135 SATMPNVAAVADWLQAALYQ------TEFRPVPLEEYI 166
SAT+PN ADW+ Q T++RP PL+ Y+
Sbjct: 106 SATVPNAKEFADWVAKVHQQPCHVVYTDYRPTPLQHYL 219
>TC232022 homologue to UP|Q9LU46 (Q9LU46) DEAD-box protein abstrakt, partial
(39%)
Length = 829
Score = 33.9 bits (76), Expect = 0.59
Identities = 27/113 (23%), Positives = 51/113 (44%), Gaps = 6/113 (5%)
Frame = +2
Query: 279 HAGLTVEEREIVETCYRKGLLRVLTATSTLAAGVNLPARRVIFRQPRIGCDFIDGTRYMQ 338
H G EERE ++ G VL AT + G++ P + + + + + +
Sbjct: 209 HGGKDQEEREYAIAAFKAGKKDVLVATDVASKGLDFPDIQHVINYD-MPAEIENYVHRIG 385
Query: 339 MAGRAGRTGIDT------KGESVLICKPQELKKVMGLLNESCPPLHSCLSEDL 385
GR G+TGI T + E+ L+ +LK ++ + PP+ + L++ +
Sbjct: 386 RTGRCGKTGIATTFINKNQSETTLL----DLKHLLQEAKQRIPPVLAELNDPM 532
>TC206536 similar to UP|Q9FZ92 (Q9FZ92) F3H9.16 protein, partial (45%)
Length = 1221
Score = 33.1 bits (74), Expect = 1.0
Identities = 22/72 (30%), Positives = 33/72 (45%)
Frame = +1
Query: 279 HAGLTVEEREIVETCYRKGLLRVLTATSTLAAGVNLPARRVIFRQPRIGCDFIDGTRYMQ 338
H G + E+REI +R VL AT G+++P + G + T +
Sbjct: 580 HGGKSQEQREISLEGFRTKRYNVLVATDVAGRGIDIPDVAHVINYDMPG-NIEMYTHRIG 756
Query: 339 MAGRAGRTGIDT 350
GRAG+TG+ T
Sbjct: 757 RTGRAGKTGVAT 792
>TC234146 homologue to UP|Q84ND9 (Q84ND9) PolI-like DNA polymerase, partial
(7%)
Length = 451
Score = 32.7 bits (73), Expect = 1.3
Identities = 16/45 (35%), Positives = 27/45 (59%)
Frame = +1
Query: 1209 LDALRVNARDYFVPTQDNWLLLTADYSQIELRLMAHFSKDSTLIE 1253
L+ R R F+ N L++ ADY Q+ELR++AH + +++E
Sbjct: 256 LEKDRYKIRQAFIAAPGNSLIV-ADYGQLELRILAHLANCKSMLE 387
>BE021424 homologue to GP|11994141|d contains similarity to DNA polymerase
I~gene_id:K10D20.8 {Arabidopsis thaliana}, partial (12%)
Length = 421
Score = 32.7 bits (73), Expect = 1.3
Identities = 16/45 (35%), Positives = 27/45 (59%)
Frame = +3
Query: 1209 LDALRVNARDYFVPTQDNWLLLTADYSQIELRLMAHFSKDSTLIE 1253
L+ R R F+ N L++ ADY Q+ELR++AH + +++E
Sbjct: 177 LEKDRYKIRQAFIAAPGNSLIV-ADYGQLELRILAHLANCKSMLE 308
>BI469188
Length = 421
Score = 32.3 bits (72), Expect = 1.7
Identities = 16/45 (35%), Positives = 27/45 (59%)
Frame = +1
Query: 1209 LDALRVNARDYFVPTQDNWLLLTADYSQIELRLMAHFSKDSTLIE 1253
L+ R R F+ N L++ ADY Q+ELR++AH + +++E
Sbjct: 232 LEKDRYKIRQAFIAAPGNSLIV-ADYGQLELRILAHLADCKSMLE 363
>CA784665
Length = 434
Score = 32.0 bits (71), Expect = 2.2
Identities = 13/22 (59%), Positives = 18/22 (81%)
Frame = +1
Query: 336 YMQMAGRAGRTGIDTKGESVLI 357
Y+QM+GRAGR GID +G +L+
Sbjct: 7 YIQMSGRAGRRGIDERGICILM 72
>TC208786 similar to UP|Q9SMX1 (Q9SMX1) Mitochondrial RNA helicase
(At4g14790), partial (49%)
Length = 1032
Score = 31.2 bits (69), Expect = 3.8
Identities = 26/81 (32%), Positives = 37/81 (45%), Gaps = 4/81 (4%)
Frame = +2
Query: 301 VLTATSTLAAGVNLPARRVIFRQPRI--GCDFIDGT--RYMQMAGRAGRTGIDTKGESVL 356
VL A+ + G+NL R+IF + G + D T Q+AGRAGR G + V
Sbjct: 140 VLVASDAIGMGLNLNISRIIFSTMKKFDGFEVRDLTVPEIKQIAGRAGRYGSNFPVGEVT 319
Query: 357 ICKPQELKKVMGLLNESCPPL 377
++L + LN P L
Sbjct: 320 CMDEEDLPLLHSSLNSPSPIL 382
>TC210165 similar to UP|Q84ND9 (Q84ND9) PolI-like DNA polymerase, partial (16%)
Length = 721
Score = 30.8 bits (68), Expect = 5.0
Identities = 21/68 (30%), Positives = 35/68 (50%), Gaps = 1/68 (1%)
Frame = +1
Query: 1437 HVNIVFLPSTSQGSAADIIKIAMIKIYSEIFPGVDKLDSSSSVTAKFEMLRDR-CRILLQ 1495
H+ + + QGSAAD+ AM++I +K + L++ ++LLQ
Sbjct: 244 HIERAAINTPVQGSAADVAMCAMLQI------------------SKNKRLKELGWKLLLQ 369
Query: 1496 VHDELVLE 1503
VHDE++LE
Sbjct: 370 VHDEVILE 393
>TC223634
Length = 578
Score = 30.8 bits (68), Expect = 5.0
Identities = 12/22 (54%), Positives = 15/22 (67%)
Frame = +1
Query: 1582 RTWGSLEPFAPDKFTGDTPIAD 1603
RTW SLEP+ DKF T +A+
Sbjct: 1 RTWXSLEPYTSDKFNNATLVAE 66
>TC209018
Length = 1688
Score = 30.4 bits (67), Expect = 6.5
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +3
Query: 1362 YLWAVCNLIMCKYIHDPDM*RLSREGNDFC 1391
Y W+ C+++ + H D+* S EGN FC
Sbjct: 831 YPWSFCSVLSFAFPHAADL*EGSEEGNIFC 920
>TC210164 similar to UP|Q84ND9 (Q84ND9) PolI-like DNA polymerase, partial (9%)
Length = 781
Score = 30.4 bits (67), Expect = 6.5
Identities = 19/67 (28%), Positives = 33/67 (48%)
Frame = +2
Query: 1437 HVNIVFLPSTSQGSAADIIKIAMIKIYSEIFPGVDKLDSSSSVTAKFEMLRDRCRILLQV 1496
H+ + + QGSAAD+ AM++I + + ++ ++LLQV
Sbjct: 107 HIERAAINTPVQGSAADVAMCAMLEISNNM-----------------KLKELGWKLLLQV 235
Query: 1497 HDELVLE 1503
HDE++LE
Sbjct: 236 HDEVILE 256
>TC225745 malate dehydrogenase
Length = 773
Score = 30.0 bits (66), Expect = 8.5
Identities = 17/59 (28%), Positives = 27/59 (44%)
Frame = -3
Query: 350 TKGESVLICKPQELKKVMGLLNESCPPLHSCLSEDLNGMTHAILEVVAGGIVQTANDIH 408
T LI KP + LN++ P H C + +NG +A ++V GI D++
Sbjct: 636 TNSSQTLIIKPWRIPT----LNKTTKPPHHCRQQYINGSENAKFDLVLKGISSNLPDMY 472
>CO981930
Length = 724
Score = 30.0 bits (66), Expect = 8.5
Identities = 18/66 (27%), Positives = 34/66 (51%), Gaps = 3/66 (4%)
Frame = +2
Query: 436 CPEESLIVLADLSRAREGFVLASDLHLVYLVTPINVDVEP---DWESYYERFVKLSPLDQ 492
C +++ +A L + F+L + HLV L P ++ P W+ YYE+ + P+++
Sbjct: 332 CSASTVVFVATLVKPHYSFLLLWNYHLVQL-NPDHMYQYPTHISWDCYYEQILLEFPVNE 508
Query: 493 SVGNRV 498
+ RV
Sbjct: 509 GLVERV 526
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.136 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 70,279,944
Number of Sequences: 63676
Number of extensions: 981187
Number of successful extensions: 4537
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 4480
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4531
length of query: 1603
length of database: 12,639,632
effective HSP length: 110
effective length of query: 1493
effective length of database: 5,635,272
effective search space: 8413461096
effective search space used: 8413461096
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 66 (30.0 bits)
Medicago: description of AC127429.13