BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC127428.6 - phase: 0 /pseudo
(322 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC218008 similar to UP|Q6NNG9 (Q6NNG9) At1g30300, partial (86%) 376 e-122
TC230514 similar to UP|Q6NNG9 (Q6NNG9) At1g30300, partial (55%) 349 1e-96
TC230513 similar to UP|Q6NNG9 (Q6NNG9) At1g30300, partial (34%) 212 2e-55
TC210051 similar to UP|Q6NNG9 (Q6NNG9) At1g30300, partial (48%) 164 7e-55
CD406300 64 9e-24
AW348999 similar to GP|9294602|dbj hydrolase-like protein {Arabi... 70 1e-12
TC230696 homologue to UP|BRU1_SOYBN (P35694) Brassinosteroid-reg... 67 1e-11
BI788317 58 5e-10
TC233980 similar to UP|Q6K3J2 (Q6K3J2) Hydrolase-like protein, p... 55 3e-08
AI461213 similar to GP|9294602|dbj| hydrolase-like protein {Arab... 44 8e-05
BM732072 35 0.046
TC210059 similar to UP|Q8S9A1 (Q8S9A1) Glucosyltransferase-8 (Fr... 28 4.3
BU761565 weakly similar to GP|17381244|gb AT3g23600/MDB19_9 {Ara... 28 7.3
TC227463 similar to UP|SYFB_ARATH (Q9SGE9) Probable phenylalanyl... 28 7.3
TC219594 similar to UP|Q8W4B1 (Q8W4B1) RNA-binding protein-like ... 27 9.5
BE611647 27 9.5
>TC218008 similar to UP|Q6NNG9 (Q6NNG9) At1g30300, partial (86%)
Length = 1428
Score = 376 bits (965), Expect(2) = e-122
Identities = 188/238 (78%), Positives = 202/238 (83%), Gaps = 1/238 (0%)
Frame = +1
Query: 15 ESALIFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNYRCNTSMLIDYCG-S 73
ESALIFLGTG S VP++ CLI+PSDPPC VC QSLS+PP NPNYRCNTS+LIDY S
Sbjct: 133 ESALIFLGTGVSGTVPSMRCLIDPSDPPCLVCTQSLSLPPHLNPNYRCNTSLLIDYYSQS 312
Query: 74 GSNHNYILIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVLGLDDVRAVQPFSPTN 133
+ H YILID GKTFRETVL SI+LTHEHADAVLGLDD+RAVQPFSPTN
Sbjct: 313 DATHKYILIDAGKTFRETVL-----------SSILLTHEHADAVLGLDDIRAVQPFSPTN 459
Query: 134 DIDPTPIYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAWNIITDDCNQPFFASGLK 193
DIDPTPIYL++HSMDS+E+ FPYLVQK+ KEGQEIRRVAQ WNII DDCN PF ASGLK
Sbjct: 460 DIDPTPIYLTRHSMDSMEKVFPYLVQKKHKEGQEIRRVAQCCWNIIADDCNLPFSASGLK 639
Query: 194 FTPLPVMHGEDYICLGFLFGEKSRVAYISDVSRIPASTEYVISKSGAGQLDLLILDSL 251
F PLPVMHGEDYICLGFLFGEK RVAYISDVSRIPASTEYVISKSGAGQLDLLILD+L
Sbjct: 640 FIPLPVMHGEDYICLGFLFGEKDRVAYISDVSRIPASTEYVISKSGAGQLDLLILDTL 813
Score = 80.9 bits (198), Expect(2) = e-122
Identities = 41/53 (77%), Positives = 43/53 (80%)
Frame = +3
Query: 259 YVTTDWIA*CSSLFSTDS*NCEEVVSKANPFDWNDS*V*SPQGQ*VFERVVQK 311
Y DWIA*CS L ST S*NCEEV+SKANP DWNDS V*SPQGQ*VF +VQK
Sbjct: 807 YTEKDWIA*CSLLSSTGS*NCEEVMSKANPIDWNDSRV*SPQGQ*VFNGLVQK 965
>TC230514 similar to UP|Q6NNG9 (Q6NNG9) At1g30300, partial (55%)
Length = 705
Score = 349 bits (895), Expect = 1e-96
Identities = 169/201 (84%), Positives = 181/201 (89%), Gaps = 2/201 (0%)
Frame = +1
Query: 1 MATSNGTDDGTPPSESALIFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNY 60
MATSNG DGT P ESALIFLG+GCSSMVPNV+CL+ PSDPPC VC QSLSIPPE+NPNY
Sbjct: 55 MATSNGFVDGTSP-ESALIFLGSGCSSMVPNVMCLLQPSDPPCPVCVQSLSIPPERNPNY 231
Query: 61 RCNTSMLIDYCGSGS--NHNYILIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVL 118
RCNTS+LIDYCG + + YILIDVGKTFRETVLRWFV HRIP++DSIILTHEHADAVL
Sbjct: 232 RCNTSLLIDYCGDANAGDRKYILIDVGKTFRETVLRWFVAHRIPRVDSIILTHEHADAVL 411
Query: 119 GLDDVRAVQPFSPTNDIDPTPIYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAWNI 178
GLDDVRAVQ FSPTNDIDPTPIYLSQHSMDSI EKFPYLVQK+RKEG EIRRVAQ+ WNI
Sbjct: 412 GLDDVRAVQAFSPTNDIDPTPIYLSQHSMDSIAEKFPYLVQKKRKEGAEIRRVAQIDWNI 591
Query: 179 ITDDCNQPFFASGLKFTPLPV 199
I DDCN+PF ASGLKFTPLPV
Sbjct: 592 IADDCNKPFLASGLKFTPLPV 654
>TC230513 similar to UP|Q6NNG9 (Q6NNG9) At1g30300, partial (34%)
Length = 486
Score = 212 bits (539), Expect = 2e-55
Identities = 103/134 (76%), Positives = 111/134 (81%), Gaps = 5/134 (3%)
Frame = +1
Query: 11 TPPSESALI--FLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNYRCNTSMLI 68
T PS I F GCSSMVPN++CL+ PSDPPC VC QSLSIPPE+NPNYRCNTS+LI
Sbjct: 85 TAPSLGICIXSFSALGCSSMVPNLMCLLQPSDPPCPVCVQSLSIPPERNPNYRCNTSLLI 264
Query: 69 DY---CGSGSNHNYILIDVGKTFRETVLRWFVHHRIPKIDSIILTHEHADAVLGLDDVRA 125
DY +G + YILIDVGKTFRETVLRWFV HRIPKIDSIILTHEHADAVLGLDD+RA
Sbjct: 265 DYRAAAANGGDRKYILIDVGKTFRETVLRWFVAHRIPKIDSIILTHEHADAVLGLDDIRA 444
Query: 126 VQPFSPTNDIDPTP 139
VQ FSPTNDIDPTP
Sbjct: 445 VQAFSPTNDIDPTP 486
>TC210051 similar to UP|Q6NNG9 (Q6NNG9) At1g30300, partial (48%)
Length = 851
Score = 164 bits (414), Expect(2) = 7e-55
Identities = 79/87 (90%), Positives = 83/87 (94%)
Frame = +2
Query: 167 EIRRVAQMAWNIITDDCNQPFFASGLKFTPLPVMHGEDYICLGFLFGEKSRVAYISDVSR 226
EIRRVAQ+ WNII DDCNQ FFASGLKFTPLPVMHGEDYICLGFLFGEK+RVAYISDVS
Sbjct: 2 EIRRVAQIDWNIIADDCNQQFFASGLKFTPLPVMHGEDYICLGFLFGEKNRVAYISDVSP 181
Query: 227 IPASTEYVISKSGAGQLDLLILDSLYR 253
+PA+TEYVISKSGAGQLDLLILDSLYR
Sbjct: 182 VPANTEYVISKSGAGQLDLLILDSLYR 262
Score = 68.2 bits (165), Expect(2) = 7e-55
Identities = 35/49 (71%), Positives = 39/49 (79%)
Frame = +1
Query: 263 DWIA*CSSLFSTDS*NCEEVVSKANPFDWNDS*V*SPQGQ*VFERVVQK 311
DW+ CS LFSTDS NCEEVVSKAN +W+DS V*SP Q*VF+ VVQK
Sbjct: 262 DWVTQCSPLFSTDSRNCEEVVSKANTTNWHDSRV*SP*RQ*VFDGVVQK 408
>CD406300
Length = 682
Score = 64.3 bits (155), Expect(2) = 9e-24
Identities = 34/48 (70%), Positives = 38/48 (78%)
Frame = -3
Query: 264 WIA*CSSLFSTDS*NCEEVVSKANPFDWNDS*V*SPQGQ*VFERVVQK 311
W+ CS LFSTDS NCEEVVS AN +W+DS*V*SP Q*VF+ VVQK
Sbjct: 581 WVTQCSPLFSTDSRNCEEVVS*ANTTNWHDS*V*SP*RQ*VFDGVVQK 438
Score = 63.5 bits (153), Expect(2) = 9e-24
Identities = 32/33 (96%), Positives = 32/33 (96%)
Frame = -1
Query: 221 ISDVSRIPASTEYVISKSGAGQLDLLILDSLYR 253
ISDVSR PASTEYVISKSGAGQLDLLILDSLYR
Sbjct: 682 ISDVSRFPASTEYVISKSGAGQLDLLILDSLYR 584
>AW348999 similar to GP|9294602|dbj hydrolase-like protein {Arabidopsis
thaliana}, partial (46%)
Length = 642
Score = 70.5 bits (171), Expect = 1e-12
Identities = 52/134 (38%), Positives = 76/134 (55%), Gaps = 1/134 (0%)
Frame = -3
Query: 119 GLDDVRAVQPFSPTNDIDP-TPIYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAWN 177
GLDD+R TN++ PIY+++ + + + YLV +V+++ +N
Sbjct: 640 GLDDLR-----DWTNNVQXHIPIYVAKRDFEVMTKTHYYLVDTSVILPGA--KVSELQFN 482
Query: 178 IITDDCNQPFFASGLKFTPLPVMHGEDYICLGFLFGEKSRVAYISDVSRIPASTEYVISK 237
II+++ PFF LKFTPLPV HG++Y LGF FG + YISDVS IP T Y + K
Sbjct: 481 IISEE---PFFVHELKFTPLPVWHGQNYRSLGFRFG---NICYISDVSDIPEET-YPLLK 323
Query: 238 SGAGQLDLLILDSL 251
++LILD+L
Sbjct: 322 ----DCEILILDAL 293
>TC230696 homologue to UP|BRU1_SOYBN (P35694) Brassinosteroid-regulated
protein BRU1 precursor , partial (33%)
Length = 1208
Score = 67.0 bits (162), Expect = 1e-11
Identities = 31/36 (86%), Positives = 33/36 (91%)
Frame = +1
Query: 113 HADAVLGLDDVRAVQPFSPTNDIDPTPIYLSQHSMD 148
HADAV+GLDDVR V FSPT+DIDPTPIYLSQHSMD
Sbjct: 820 HADAVVGLDDVRDVLAFSPTSDIDPTPIYLSQHSMD 927
>BI788317
Length = 420
Score = 58.2 bits (139), Expect(2) = 5e-10
Identities = 33/52 (63%), Positives = 37/52 (70%)
Frame = +2
Query: 260 VTTDWIA*CSSLFSTDS*NCEEVVSKANPFDWNDS*V*SPQGQ*VFERVVQK 311
V DWI S L STD *NC+ V+SKANP D N+S V*SPQGQ VF +VQK
Sbjct: 38 VGKDWIIQRSLLSSTDY*NCKVVMSKANPIDRNESQV*SPQGQRVFNGMVQK 193
Score = 23.1 bits (48), Expect(2) = 5e-10
Identities = 10/11 (90%), Positives = 11/11 (99%)
Frame = +3
Query: 241 GQLDLLILDSL 251
GQLDLLILD+L
Sbjct: 9 GQLDLLILDAL 41
>TC233980 similar to UP|Q6K3J2 (Q6K3J2) Hydrolase-like protein, partial (19%)
Length = 421
Score = 55.5 bits (132), Expect = 3e-08
Identities = 33/69 (47%), Positives = 40/69 (57%)
Frame = +3
Query: 15 ESALIFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNYRCNTSMLIDYCGSG 74
++ LIFLGTG S VP V CL NP C VC+++ N N R NTS+LI + S
Sbjct: 225 QTELIFLGTGTSEGVPRVSCLTNPL-LKCEVCSKAAQ---PGNKNRRLNTSILIRHPNSS 392
Query: 75 SNHNYILID 83
HN ILID
Sbjct: 393 GTHNNILID 419
>AI461213 similar to GP|9294602|dbj| hydrolase-like protein {Arabidopsis
thaliana}, partial (25%)
Length = 432
Score = 44.3 bits (103), Expect = 8e-05
Identities = 21/40 (52%), Positives = 24/40 (59%)
Frame = +3
Query: 57 NPNYRCNTSMLIDYCGSGSNHNYILIDVGKTFRETVLRWF 96
N N R NTS+LI + S HN ILID GK F + L WF
Sbjct: 288 NKNRRLNTSILIRHPNSSGTHNNILIDAGKFFYHSALHWF 407
Score = 33.5 bits (75), Expect = 0.13
Identities = 15/24 (62%), Positives = 17/24 (70%)
Frame = +1
Query: 15 ESALIFLGTGCSSMVPNVLCLINP 38
++ LIFLGTG S VP V CL NP
Sbjct: 52 QTELIFLGTGTSEGVPRVSCLTNP 123
>BM732072
Length = 423
Score = 35.0 bits (79), Expect = 0.046
Identities = 17/28 (60%), Positives = 19/28 (67%), Gaps = 2/28 (7%)
Frame = +1
Query: 255 LSFFY--VTTDWIA*CSSLFSTDS*NCE 280
+SFF V DW+ CS LFSTDS NCE
Sbjct: 340 VSFFVLCVIADWVTQCSPLFSTDSRNCE 423
>TC210059 similar to UP|Q8S9A1 (Q8S9A1) Glucosyltransferase-8 (Fragment),
partial (37%)
Length = 803
Score = 28.5 bits (62), Expect = 4.3
Identities = 16/38 (42%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Frame = -2
Query: 27 SMVPNVLCLINPSDPPCSVCAQ-SLSIPPEKNPNYRCN 63
S VP+ LCL++ DPP S+ + L P +PN R N
Sbjct: 490 SSVPHFLCLLSNHDPPHSLLNRFLLHRVPSHHPNPRLN 377
>BU761565 weakly similar to GP|17381244|gb AT3g23600/MDB19_9 {Arabidopsis
thaliana}, partial (60%)
Length = 447
Score = 27.7 bits (60), Expect = 7.3
Identities = 11/50 (22%), Positives = 21/50 (42%)
Frame = +2
Query: 127 QPFSPTNDIDPTPIYLSQHSMDSIEEKFPYLVQKQRKEGQEIRRVAQMAW 176
+PF+ N P P++L H D E +++ + +G + W
Sbjct: 29 EPFNYQNSNRPLPVWLKDHGPDKGSEATKPIIEALKSKGVSVIAAVGFCW 178
>TC227463 similar to UP|SYFB_ARATH (Q9SGE9) Probable phenylalanyl-tRNA
synthetase beta chain (Phenylalanine--tRNA ligase beta
chain) (PheRS) , partial (10%)
Length = 676
Score = 27.7 bits (60), Expect = 7.3
Identities = 20/56 (35%), Positives = 26/56 (45%)
Frame = -1
Query: 227 IPASTEYVISKSGAGQLDLLILDSLYRFLSFFYVTTDWIA*CSSLFSTDS*NCEEV 282
IP E + SK GQ+ L LYR LS T + + S LF+T * +V
Sbjct: 364 IPQFDE*IYSKGRMGQMGLPTDTDLYRKLSMLSSTKEQGSGMSKLFNTSG*TMPKV 197
>TC219594 similar to UP|Q8W4B1 (Q8W4B1) RNA-binding protein-like (At5g53060),
partial (13%)
Length = 852
Score = 27.3 bits (59), Expect = 9.5
Identities = 13/30 (43%), Positives = 18/30 (59%)
Frame = -2
Query: 62 CNTSMLIDYCGSGSNHNYILIDVGKTFRET 91
CN+ ++ CGS SN NYI +G+ R T
Sbjct: 239 CNSLQILIGCGSFSNRNYI---IGRRCRRT 159
>BE611647
Length = 410
Score = 27.3 bits (59), Expect = 9.5
Identities = 16/54 (29%), Positives = 26/54 (47%)
Frame = +3
Query: 12 PPSESALIFLGTGCSSMVPNVLCLINPSDPPCSVCAQSLSIPPEKNPNYRCNTS 65
PPS +A ++ P+ L + + PP S + PP ++P RC+TS
Sbjct: 48 PPSPTASSRNAAASATASPSSNPLPSSTGPPLSTAS-----PPRRSPTTRCSTS 194
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.332 0.145 0.468
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,585,639
Number of Sequences: 63676
Number of extensions: 271524
Number of successful extensions: 1706
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 1650
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1699
length of query: 322
length of database: 12,639,632
effective HSP length: 97
effective length of query: 225
effective length of database: 6,463,060
effective search space: 1454188500
effective search space used: 1454188500
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 59 (27.3 bits)
Medicago: description of AC127428.6