BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC127428.4 + phase: 0
(358 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC212187 weakly similar to UP|Q6USA0 (Q6USA0) Transposase (Fragm... 125 2e-46
TC231143 134 5e-32
TC224534 similar to UP|Q6USA0 (Q6USA0) Transposase (Fragment), p... 134 6e-32
TC231809 100 8e-22
TC234723 similar to UP|Q9FHY5 (Q9FHY5) Emb|CAB72466.1 (At5g41980... 97 9e-21
BQ742132 weakly similar to GP|28416671|gb| At5g41980 {Arabidopsi... 76 3e-14
AW396897 similar to GP|28416671|gb| At5g41980 {Arabidopsis thali... 69 3e-12
TC210984 67 1e-11
TC214571 weakly similar to UP|Q9ZSW3 (Q9ZSW3) CAK associated cyc... 31 0.99
TC211744 weakly similar to UP|O46921 (O46921) Thylakoid-bound as... 29 2.9
TC209445 similar to GB|AAB63625.1|2262117|ATAC002343 auxin induc... 28 4.9
CD413373 28 6.4
TC224955 UP|Q8JUF5 (Q8JUF5) Polyprotein 1, partial (44%) 28 8.4
TC220792 weakly similar to UP|Q84ME1 (Q84ME1) At5g19080/T16G12_1... 28 8.4
>TC212187 weakly similar to UP|Q6USA0 (Q6USA0) Transposase (Fragment),
partial (45%)
Length = 973
Score = 125 bits (313), Expect(2) = 2e-46
Identities = 72/191 (37%), Positives = 102/191 (52%), Gaps = 29/191 (15%)
Frame = +1
Query: 188 KALTTANLNFPHPPQG---------------------------KYYLVDSGYPTPIGYIG 220
+AL L+FPHPPQG +YLVDS YPT +G++G
Sbjct: 382 EALRKPALHFPHPPQGT*NLYIINICIIIILCSNMLYCFIL*VNFYLVDSCYPTFMGFLG 561
Query: 221 PYRCERYHLPEFRRSSGFENHNEVFNYYHSSLRCTIERTFGVWKNRFAILRSMPKFKYET 280
Y RYHLP+FR EVFNYYHSSL+ TIER FG+ K R+ IL +M F +T
Sbjct: 562 SYNKTRYHLPQFRIGPRIRGRVEVFNYYHSSLQSTIERAFGLCKARWKILGNMSPFALKT 741
Query: 281 QVHIVVATMAIHNFIRRSAEMDVDFNLYEDENTVIHHDDDHRSTNLNQS--QSFNVASSS 338
Q I+VA M IHNFI+R+ + D +F+ +++N I D D + + + + S+
Sbjct: 742 QNQIIVACMTIHNFIQRNDKSDGEFDSLDEDNEYIDTDKDESEVGPSTTTWEESDAQSTL 921
Query: 339 EMDHARNSIRD 349
M+ R S+++
Sbjct: 922 LMERFRESLKN 954
Score = 79.0 bits (193), Expect(2) = 2e-46
Identities = 33/61 (54%), Positives = 44/61 (72%)
Frame = +2
Query: 126 ETVRVIDGTHVSCVVSASEQPRFIGRKGYPTQNIMAVCDWNMCFTFVLAGWEGTAHDARV 185
+ + IDGTH+ V + Q + GRKGY T N+M VCD++MCFTFV AGWEG+AHD ++
Sbjct: 197 DCIGAIDGTHIRVCVPSHLQGVYSGRKGYTTTNVMIVCDFSMCFTFVWAGWEGSAHDTKI 376
Query: 186 F 186
F
Sbjct: 377 F 379
>TC231143
Length = 941
Score = 134 bits (338), Expect = 5e-32
Identities = 81/185 (43%), Positives = 106/185 (56%), Gaps = 25/185 (13%)
Frame = +3
Query: 1 MNFWKEQLEHNTQEEEDDDTFEESYILAALLGEYATKYLCKEPCRTSELTGHAWVQEILQ 60
MN + EQLE NT E DD+ E++ + +GEYA K+LCKE CRT++ GHAWVQE+LQ
Sbjct: 396 MNDFLEQLESNTNE--DDELMEQASYVVVFIGEYAIKHLCKEICRTNKQIGHAWVQEVLQ 569
Query: 61 GNPTRCYEMFRMEKHIFHKLCHELVEHDLKSSKHM-----GVEEMVAMFLVVVGHGV--- 112
G+P CYEMF MEKHIF+ LC +LV+H +K +K++ EE ++ L V +
Sbjct: 570 GHPIHCYEMFCMEKHIFYMLCXKLVDH-VKGNKNLQERF*DSEENMSRHLHEVLIALLKL 746
Query: 113 ---GNRMIQERFQHS--------------GETVRVIDGTHVSCVVSASEQPRFIGRKGYP 155
R + F+ S + DGTHV C+ SEQ RFI RKG P
Sbjct: 747 SMEXXRPLDPLFRDSHAKIENDTWYWPFFKNVIGTNDGTHVPCMXXHSEQARFIRRKGIP 926
Query: 156 TQNIM 160
TQNIM
Sbjct: 927 TQNIM 941
>TC224534 similar to UP|Q6USA0 (Q6USA0) Transposase (Fragment), partial (50%)
Length = 968
Score = 134 bits (337), Expect = 6e-32
Identities = 79/234 (33%), Positives = 125/234 (52%), Gaps = 7/234 (2%)
Frame = +1
Query: 127 TVRVIDGTHVSCVVSASEQPRFIGRKGYPTQNIMAVCDWNMCFTFVLAGWEGTAHDARVF 186
++ +DG H+ V+A ++PR+ RKG N++ C N+ F +VL GWEG+A D+RV
Sbjct: 253 SIGALDGIHIPVTVTAEDRPRYHNRKGDIFTNVLGACGPNLRFIYVLPGWEGSAGDSRVL 432
Query: 187 DKALTTANLNFPHPPQGKYYLVDSGYPTPIGYIGPYRCERYHLPEFRRSSGFENHNEVFN 246
AL N H P GKY+LVD+GY G++ PY RYH E+ ++ +N+ E+FN
Sbjct: 433 *DALHRQNC--LHIPNGKYFLVDAGYTNGPGFLTPY*RTRYHRNEWIGNTP-QNYKELFN 603
Query: 247 YYHSSLRCTIERTFGVWKNRFAILRSMPKFKYETQVHIVVATMAIHNFIRRSAEMDVDFN 306
H+S + IER+FGV K R++ILR+ F +TQ+ I+ + NFIR
Sbjct: 604 LRHASAKNVIERSFGVLKKRWSILRTPSFFDIKTQIRIINTCFMLRNFIR---------- 753
Query: 307 LYEDENTVIHHDDDHRSTNLNQSQSFNVAS-------SSEMDHARNSIRDQIIA 353
D+ +S + ++Q + S + +M+ N+IRD++ A
Sbjct: 754 ------------DEQQSDPILEAQDLELLSVVDNELINQQMERVTNNIRDEVTA 879
>TC231809
Length = 760
Score = 100 bits (250), Expect = 8e-22
Identities = 56/133 (42%), Positives = 72/133 (54%), Gaps = 1/133 (0%)
Frame = +3
Query: 175 GWEGTAHDARVFDKALTTANLNFPHPPQGKYYLVDSGYPTPIGYIGPYRCERYHLPEFRR 234
G EG+ D V L + P PQGKYYLV GY G+I PY+ RY EFR
Sbjct: 3 GGEGSVXDXXVLXXVLDXXDQXXPQIPQGKYYLVXKGYLNTEGFIAPYQGVRYQHYEFRG 182
Query: 235 SSGF-ENHNEVFNYYHSSLRCTIERTFGVWKNRFAILRSMPKFKYETQVHIVVATMAIHN 293
++ N E+FN+ H LR I R+F V K RF IL+ P++ ++ Q IV+A +HN
Sbjct: 183 ANQLPRNAKELFNHRHCFLRNAILRSFNVLKTRFPILKLAPQYSFQIQRDIVIAGCVLHN 362
Query: 294 FIRRSAEMDVDFN 306
FIRR D FN
Sbjct: 363 FIRREERNDWIFN 401
>TC234723 similar to UP|Q9FHY5 (Q9FHY5) Emb|CAB72466.1 (At5g41980), partial
(24%)
Length = 837
Score = 97.4 bits (241), Expect = 9e-21
Identities = 49/108 (45%), Positives = 66/108 (60%)
Frame = +2
Query: 203 GKYYLVDSGYPTPIGYIGPYRCERYHLPEFRRSSGFENHNEVFNYYHSSLRCTIERTFGV 262
GKYY+VDS Y G++ PY Y+ EF ++ +E+FN HS LR I+RTFG+
Sbjct: 137 GKYYIVDSKYQNVPGFVAPYSSTPYYSKEFLSDYHPQDASELFNQRHSLLRHVIDRTFGI 316
Query: 263 WKNRFAILRSMPKFKYETQVHIVVATMAIHNFIRRSAEMDVDFNLYED 310
K RF IL S P + +TQV +VVA A+HN+IRR D F +YE+
Sbjct: 317 LKARFPILMSAPSYPLQTQVKLVVAACALHNYIRREKPDDWLFKMYEE 460
>BQ742132 weakly similar to GP|28416671|gb| At5g41980 {Arabidopsis thaliana},
partial (25%)
Length = 429
Score = 75.9 bits (185), Expect = 3e-14
Identities = 37/71 (52%), Positives = 50/71 (70%), Gaps = 1/71 (1%)
Frame = +3
Query: 59 LQGNPTRCYEMFRMEKHIFHKLCHELVEHD-LKSSKHMGVEEMVAMFLVVVGHGVGNRMI 117
L G+ C EM RM+KH+FHKLC L + L+ + + +EE +A+FL V+GH NR+I
Sbjct: 9 LNGHGDFCREMLRMDKHVFHKLCDILRQRAMLRDTAGVMIEEQLAIFLNVIGHNERNRVI 188
Query: 118 QERFQHSGETV 128
QERFQHSGET+
Sbjct: 189 QERFQHSGETI 221
>AW396897 similar to GP|28416671|gb| At5g41980 {Arabidopsis thaliana},
partial (24%)
Length = 430
Score = 69.3 bits (168), Expect = 3e-12
Identities = 39/118 (33%), Positives = 63/118 (53%), Gaps = 1/118 (0%)
Frame = +2
Query: 12 TQEEEDDDTFEESYILAALLGEYATKYLCKEPCRTSELTGHAWVQEILQGNPTRCYEMFR 71
+ EEE+D + G +ATK L G ++ E+L G C E FR
Sbjct: 89 SSEEEND----------GVSGNHATKELIHLASN-----GAKFIDEVLNGQNECCLENFR 223
Query: 72 MEKHIFHKLCHELVEHDL-KSSKHMGVEEMVAMFLVVVGHGVGNRMIQERFQHSGETV 128
M+KH+F+KLC L L + + + +EE + +F+ ++GH + R +QE F++SGET+
Sbjct: 224 MDKHVFYKLCDILQAKGLLRHTNRIKIEEQLGIFMFIIGHNLRTRAVQELFRYSGETI 397
>TC210984
Length = 1085
Score = 67.4 bits (163), Expect = 1e-11
Identities = 37/94 (39%), Positives = 55/94 (58%), Gaps = 6/94 (6%)
Frame = +3
Query: 243 EVFNYYHSSLRCTIERTFGVWKNRFAILRSMPKFKYETQVHIVVATMAIHNFIR---RSA 299
E+FN H+SLR IER FG++K+RF I +S P F ++TQ +V+A A+HNF+R RS
Sbjct: 426 ELFNLRHASLRNVIERIFGIFKSRFTIFKSAPPFLFKTQAELVLACAALHNFLRKECRSD 605
Query: 300 EMDVDFNLYEDENTVI---HHDDDHRSTNLNQSQ 330
E V+ ++ + + D+DH Q Q
Sbjct: 606 EFPVEPTDESSSSSSVLPNYEDNDHEPIVQTQEQ 707
>TC214571 weakly similar to UP|Q9ZSW3 (Q9ZSW3) CAK associated cyclinH
homolog, partial (37%)
Length = 798
Score = 30.8 bits (68), Expect = 0.99
Identities = 15/47 (31%), Positives = 28/47 (58%), Gaps = 1/47 (2%)
Frame = -1
Query: 59 LQGNPTRCYEMFRMEKHIF-HKLCHELVEHDLKSSKHMGVEEMVAMF 104
L +P RCY++FRM+K F +++ + K++G+ E V++F
Sbjct: 798 LNHHPMRCYQIFRMKKICFLNQVTYCKASTT*SQLKNVGIHEQVSLF 658
>TC211744 weakly similar to UP|O46921 (O46921) Thylakoid-bound ascorbate
peroxidase, partial (6%)
Length = 305
Score = 29.3 bits (64), Expect = 2.9
Identities = 15/59 (25%), Positives = 33/59 (55%), Gaps = 2/59 (3%)
Frame = +3
Query: 293 NFIRRSAEMDVDFNLYEDENTVIHHDDDHRSTNLNQS--QSFNVASSSEMDHARNSIRD 349
NFI+R+ + D +FN +++N I D+D + + + + S+ +M+ R S+++
Sbjct: 21 NFIQRNDKSDGEFNSLDEDNADIDSDEDGSEVGPSTTTWEEPDAQSTLQMERFRESLKN 197
>TC209445 similar to GB|AAB63625.1|2262117|ATAC002343 auxin inducible protein
isolog {Arabidopsis thaliana;} , partial (18%)
Length = 996
Score = 28.5 bits (62), Expect = 4.9
Identities = 14/52 (26%), Positives = 25/52 (47%), Gaps = 2/52 (3%)
Frame = -3
Query: 39 LCKEPCRTSELTGHAWVQ--EILQGNPTRCYEMFRMEKHIFHKLCHELVEHD 88
LC PC+ + H + E+L+ RC +H+LCH+++E +
Sbjct: 385 LC*SPCQPGQGNDHCSISLSEVLK*AKLRC--------SCYHRLCHDIIEKE 254
>CD413373
Length = 667
Score = 28.1 bits (61), Expect = 6.4
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +1
Query: 323 STNLNQSQSFNVASSSEMDHARNSIRDQIIAYKL 356
S+ L SQS + SS ++ AR S+ ++ AYKL
Sbjct: 400 SSELPSSQSSSSNSSEDLSRARLSVHSRLSAYKL 501
>TC224955 UP|Q8JUF5 (Q8JUF5) Polyprotein 1, partial (44%)
Length = 2743
Score = 27.7 bits (60), Expect = 8.4
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +1
Query: 16 EDDDTFEESYILAALLGEYATKYLCKEPCRTSE 48
+++D+FEE + AAL G Y+ K P TSE
Sbjct: 643 DEEDSFEEVSLSAALNGVEGLDYMEKIPLATSE 741
>TC220792 weakly similar to UP|Q84ME1 (Q84ME1) At5g19080/T16G12_120, partial
(44%)
Length = 1156
Score = 27.7 bits (60), Expect = 8.4
Identities = 11/30 (36%), Positives = 16/30 (52%)
Frame = +3
Query: 198 PHPPQGKYYLVDSGYPTPIGYIGPYRCERY 227
P PPQG + + Y + GY+GP + Y
Sbjct: 237 PPPPQGYFIASTNPYASTTGYVGPPPTQYY 326
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.136 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,642,621
Number of Sequences: 63676
Number of extensions: 299396
Number of successful extensions: 1319
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 1307
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1313
length of query: 358
length of database: 12,639,632
effective HSP length: 98
effective length of query: 260
effective length of database: 6,399,384
effective search space: 1663839840
effective search space used: 1663839840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC127428.4