BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC127169.10 + phase: 1 /pseudo/partial
(367 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC225216 homologue to UP|Q6PW04 (Q6PW04) Membrane-anchored endo-... 55 5e-08
TC225224 similar to UP|Q6PW04 (Q6PW04) Membrane-anchored endo-1,... 52 3e-07
TC225220 similar to UP|Q6PW04 (Q6PW04) Membrane-anchored endo-1,... 51 9e-07
TC225223 similar to UP|Q6QLN2 (Q6QLN2) Endo-1,4-beta-glucanase ... 51 9e-07
TC225217 homologue to UP|Q6PW04 (Q6PW04) Membrane-anchored endo-... 48 8e-06
TC226013 similar to UP|Q8L6S6 (Q8L6S6) Endo-beta-1,4-glucanase ... 32 0.45
BQ610423 similar to PIR|T07612|T076 cellulase (EC 3.2.1.4) Cel3 ... 31 0.77
TC223402 weakly similar to UP|Q9SFV8 (Q9SFV8) T1B9.5 protein, pa... 31 1.0
TC205687 similar to PIR|E96786|E96786 protein F10A5.13 [imported... 30 1.3
BQ741939 30 2.2
NP005443 cellulase 29 3.8
TC210032 similar to UP|Q41012 (Q41012) Endo-1,4-beta-glucanase p... 28 5.0
CF920945 28 8.5
>TC225216 homologue to UP|Q6PW04 (Q6PW04) Membrane-anchored
endo-1,4-beta-glucanase , complete
Length = 2204
Score = 55.1 bits (131), Expect = 5e-08
Identities = 27/74 (36%), Positives = 41/74 (54%), Gaps = 10/74 (13%)
Frame = +2
Query: 40 QLNIRQNTVNRYDHARELIKWGTDYLLLTFDSSASEINQIHAKVG-GSLKGSE------- 91
+ + + +H +E+IKWGTDY L +F+S+A I + A+VG G G +
Sbjct: 551 EYSAKYEAAGELEHVKEIIKWGTDYFLKSFNSTADTITTLAAQVGLGDTSGGDSPNDHYC 730
Query: 92 --KPEDMEYPRPTT 103
+PEDM+Y RP T
Sbjct: 731 WMRPEDMDYDRPVT 772
Score = 49.7 bits (117), Expect = 2e-06
Identities = 28/57 (49%), Positives = 37/57 (64%)
Frame = +2
Query: 311 YDLRNNNSYTEPTLAGNAGLVAALISLTSTSTIGSGFDTDHTIFNQTRQYRPQNLPP 367
+D+R N +YTEPTLAGNAGLVAAL++L+ G+ D +TIF+ P PP
Sbjct: 1667 HDVRTNYNYTEPTLAGNAGLVAALVALSGDK--GTSID-KNTIFSAVPPMFPTPPPP 1828
Score = 37.4 bits (85), Expect(2) = 8e-04
Identities = 29/88 (32%), Positives = 44/88 (49%)
Frame = +1
Query: 219 WLPLRGNFEYVP*IY*PYHVLLTKIVVAIIKICRGIYTIEPWIPKFSPICC*CFIYGFSF 278
W+ + NFE + HVLL+ + + R + +I+PW SPIC C ++G
Sbjct: 1174 WVSI*RNFEDLSQSDKHNHVLLSTSFHKL*QNQRWLDSIKPW*ASASPICGQCGLFGCPI 1353
Query: 279 C*LYESPRCSWMVL*S*LLFNICSKGIC 306
*L +WMVL +* LFN + +C
Sbjct: 1354 **LS*CC*YTWMVLWT*FLFNRRAP*LC 1437
Score = 22.7 bits (47), Expect(2) = 8e-04
Identities = 8/13 (61%), Positives = 13/13 (99%)
Frame = +2
Query: 205 LLLTRVRMFLNPG 217
+LL+R+R+FL+PG
Sbjct: 1139 VLLSRLRLFLSPG 1177
>TC225224 similar to UP|Q6PW04 (Q6PW04) Membrane-anchored
endo-1,4-beta-glucanase , partial (54%)
Length = 1078
Score = 52.4 bits (124), Expect = 3e-07
Identities = 27/63 (42%), Positives = 39/63 (61%), Gaps = 11/63 (17%)
Frame = +2
Query: 53 HARELIKWGTDYLLLTFDSSASEINQIHAKVGG--SLKGSE---------KPEDMEYPRP 101
H +++IKWGTDYLL F+S+A I Q+ +VG + +GS +PED++Y RP
Sbjct: 650 HVKDIIKWGTDYLLKNFNSTADTITQLGMQVGSGDTSQGSTTPNDHYCWMRPEDIDYDRP 829
Query: 102 TTT 104
T T
Sbjct: 830 TQT 838
>TC225220 similar to UP|Q6PW04 (Q6PW04) Membrane-anchored
endo-1,4-beta-glucanase , partial (31%)
Length = 573
Score = 50.8 bits (120), Expect = 9e-07
Identities = 26/59 (44%), Positives = 36/59 (60%), Gaps = 10/59 (16%)
Frame = +2
Query: 55 RELIKWGTDYLLLTFDSSASEINQIHAKVG-GSLKGSE---------KPEDMEYPRPTT 103
+E+IKWGTDY L +F+S+A I + A+VG G G + +PEDM+Y RP T
Sbjct: 5 KEIIKWGTDYFLKSFNSTADTITTLAAQVGLGDTSGGDSPNDHYCWMRPEDMDYDRPVT 181
>TC225223 similar to UP|Q6QLN2 (Q6QLN2) Endo-1,4-beta-glucanase , partial
(61%)
Length = 1470
Score = 50.8 bits (120), Expect = 9e-07
Identities = 27/57 (47%), Positives = 36/57 (62%)
Frame = +3
Query: 311 YDLRNNNSYTEPTLAGNAGLVAALISLTSTSTIGSGFDTDHTIFNQTRQYRPQNLPP 367
+D+R N +YTEPTLAGNAGLVAAL++L+ T G +T+F+ P PP
Sbjct: 957 HDVRTNYNYTEPTLAGNAGLVAALVALSGDKTTGI---DKNTLFSAVPPMFPTPPPP 1118
Score = 39.3 bits (90), Expect(2) = 2e-04
Identities = 29/91 (31%), Positives = 41/91 (44%)
Frame = +2
Query: 216 PG*WLPLRGNFEYVP*IY*PYHVLLTKIVVAIIKICRGIYTIEPWIPKFSPICC*CFIYG 275
P WL L N ++ HVLL V + + +I+PW SPICC C ++G
Sbjct: 455 PQSWLSL*RNSNHISQSDRHSHVLLPPNVHQL**NKGWLDSIKPWQASASPICCQCSLFG 634
Query: 276 FSFC*LYESPRCSWMVL*S*LLFNICSKGIC 306
*L +WMVL + L + C +C
Sbjct: 635 CPLQ*LSRCC*YTWMVLWTQFLLD*CPSRLC 727
Score = 22.7 bits (47), Expect(2) = 2e-04
Identities = 8/13 (61%), Positives = 13/13 (99%)
Frame = +3
Query: 205 LLLTRVRMFLNPG 217
+LL+R+R+FL+PG
Sbjct: 429 VLLSRLRLFLSPG 467
>TC225217 homologue to UP|Q6PW04 (Q6PW04) Membrane-anchored
endo-1,4-beta-glucanase , partial (25%)
Length = 830
Score = 47.8 bits (112), Expect = 8e-06
Identities = 26/57 (45%), Positives = 35/57 (60%)
Frame = +1
Query: 311 YDLRNNNSYTEPTLAGNAGLVAALISLTSTSTIGSGFDTDHTIFNQTRQYRPQNLPP 367
+D+R N +YTEPTLAGNAGLVAAL++L+ + +TIF+ P PP
Sbjct: 283 HDVRTNYNYTEPTLAGNAGLVAALVALSGDKSTSI---DKNTIFSAVPPMFPTPPPP 444
>TC226013 similar to UP|Q8L6S6 (Q8L6S6) Endo-beta-1,4-glucanase , partial
(96%)
Length = 2302
Score = 32.0 bits (71), Expect = 0.45
Identities = 23/66 (34%), Positives = 31/66 (46%), Gaps = 5/66 (7%)
Frame = +2
Query: 53 HARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGS---EKPEDMEYPRPTTTI*SEG 109
HA E +KWGTDY F + + N ++ +VG ++PEDM R I
Sbjct: 452 HAMEAVKWGTDY----FIKAHPQPNVLYGEVGDGNTDHYCWQRPEDMTTDRHAYKIDPSN 619
Query: 110 P--DLA 113
P DLA
Sbjct: 620 PGSDLA 637
>BQ610423 similar to PIR|T07612|T076 cellulase (EC 3.2.1.4) Cel3
membrane-anchored - tomato, partial (7%)
Length = 435
Score = 31.2 bits (69), Expect = 0.77
Identities = 18/42 (42%), Positives = 23/42 (53%)
Frame = +2
Query: 326 GNAGLVAALISLTSTSTIGSGFDTDHTIFNQTRQYRPQNLPP 367
GNAGLVAAL++L+ T G F T+F+ P PP
Sbjct: 149 GNAGLVAALVALSGDKTTGIDF---FTLFSAVPPVFPTPPPP 265
>TC223402 weakly similar to UP|Q9SFV8 (Q9SFV8) T1B9.5 protein, partial (11%)
Length = 699
Score = 30.8 bits (68), Expect = 1.0
Identities = 12/48 (25%), Positives = 24/48 (50%)
Frame = -2
Query: 159 MSTCGVVFGYFWPLVTTPTFPWQWQPILLCLRMPKHLIRLPILISYLL 206
+ +C + + + + P+FP+ + P LLCL P L + ++ L
Sbjct: 155 LCSCPCLSFFLYVFLFCPSFPYAFHPFLLCLSFPSSLCPSSLFYAFFL 12
>TC205687 similar to PIR|E96786|E96786 protein F10A5.13 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(55%)
Length = 1314
Score = 30.4 bits (67), Expect = 1.3
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = +2
Query: 307 NFSVYDLRNNNSYTEPTLAGNAGLVAALISLTSTSTIGSGF 347
N + D RNN+ TEP+ +A +V L SL +TS+ F
Sbjct: 860 NETFIDSRNNSMQTEPSTYNSAVIVGLLSSLVTTSSAVQSF 982
>BQ741939
Length = 432
Score = 29.6 bits (65), Expect = 2.2
Identities = 12/35 (34%), Positives = 20/35 (56%)
Frame = +3
Query: 172 LVTTPTFPWQWQPILLCLRMPKHLIRLPILISYLL 206
L+ PT W P+LL + +P HL++ ++ LL
Sbjct: 258 LLLLPTISWTTMPLLLIIMLPPHLLQFLHILLLLL 362
>NP005443 cellulase
Length = 653
Score = 28.9 bits (63), Expect = 3.8
Identities = 17/65 (26%), Positives = 28/65 (42%)
Frame = +1
Query: 46 NTVNRYDHARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGSEKPEDMEYPRPTTTI 105
++VN+ + I WG D++L S + Q+ E+PEDM+ R I
Sbjct: 355 SSVNQLGYLHSAIHWGADFILRAHTSPTTLYTQV-GDGNADHNCWERPEDMDTARAVYKI 531
Query: 106 *SEGP 110
+ P
Sbjct: 532 DANSP 546
>TC210032 similar to UP|Q41012 (Q41012) Endo-1,4-beta-glucanase precursor ,
partial (58%)
Length = 929
Score = 28.5 bits (62), Expect = 5.0
Identities = 17/47 (36%), Positives = 22/47 (46%)
Frame = +2
Query: 54 ARELIKWGTDYLLLTFDSSASEINQIHAKVGGSLKGSEKPEDMEYPR 100
AR I+W TDYLL S+ + K E+PEDM+ R
Sbjct: 380 ARAAIRWATDYLLKCATSTPGRLYVGVGDPNVDHKCWERPEDMDTVR 520
>CF920945
Length = 758
Score = 27.7 bits (60), Expect = 8.5
Identities = 17/52 (32%), Positives = 25/52 (47%)
Frame = +2
Query: 151 IITHLATLMSTCGVVFGYFWPLVTTPTFPWQWQPILLCLRMPKHLIRLPILI 202
II ++ M C + FG WP +T P F + L ++ H+IR I I
Sbjct: 455 IINSVSKFMVICYLDFGTGWPHLTPPKFFYDLSLGNLSVQQGCHVIRSNIAI 610
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.343 0.153 0.541
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,396,805
Number of Sequences: 63676
Number of extensions: 339924
Number of successful extensions: 3095
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 3066
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3089
length of query: 367
length of database: 12,639,632
effective HSP length: 99
effective length of query: 268
effective length of database: 6,335,708
effective search space: 1697969744
effective search space used: 1697969744
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.5 bits)
S2: 60 (27.7 bits)
Medicago: description of AC127169.10