BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC127021.6 + phase: 0 /pseudo
(408 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC234877 69 5e-12
TC211561 weakly similar to UP|O22675 (O22675) Reverse transcript... 68 6e-12
TC222348 67 2e-11
CO982349 62 5e-10
TC221865 60 1e-09
BE610670 60 2e-09
TC233956 60 2e-09
TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR ret... 58 7e-09
BI973267 58 7e-09
CD396188 weakly similar to GP|6175788|gb|A ORF144 {Xestia c-nigr... 58 7e-09
TC231922 58 9e-09
TC222857 58 9e-09
CA851073 58 9e-09
BQ453949 56 3e-08
BI702137 55 4e-08
CF921567 55 6e-08
TC233392 54 2e-07
BG404993 53 2e-07
BQ629929 53 2e-07
CO984521 53 3e-07
>TC234877
Length = 534
Score = 68.6 bits (166), Expect = 5e-12
Identities = 33/78 (42%), Positives = 48/78 (61%)
Frame = +1
Query: 1 DRIKSKLSTWHGRLLSIMGRAQLVNYVISGMLVYTFHIHRWPKIILNEITKIIRNFVWSG 60
DRIK++L +W G LLS MG QL+ +I GML+ +FH++ WPK +L E+ RNF+W+
Sbjct: 109 DRIKAELQSWKGNLLSFMGCVQLIISIIDGMLL*SFHLYSWPKALLIEMQTWTRNFIWTR 288
Query: 61 DCNQQKICTVAWNTMCRP 78
++K W C P
Sbjct: 289 GYFKEK-TGYCWLKNCVP 339
>TC211561 weakly similar to UP|O22675 (O22675) Reverse transcriptase
(Fragment) , partial (59%)
Length = 1077
Score = 68.2 bits (165), Expect = 6e-12
Identities = 31/88 (35%), Positives = 52/88 (58%)
Frame = +1
Query: 2 RIKSKLSTWHGRLLSIMGRAQLVNYVISGMLVYTFHIHRWPKIILNEITKIIRNFVWSGD 61
+ + KLS W R +S GR L+ V++ + +Y F R P+ +++++ KI R F+W G
Sbjct: 661 KCERKLSKWK*RHISFGGRVTLIKSVLTSIPIYFFSFFRVPQSVVDKLVKI*RTFLWGGG 840
Query: 62 CNQQKICTVAWNTMCRPKDEGGLAIRDP 89
+Q+KI + W +C PK+ GG+ R P
Sbjct: 841 PDQKKISWIRWEKLCLPKERGGI*KRLP 924
>TC222348
Length = 995
Score = 66.6 bits (161), Expect = 2e-11
Identities = 34/98 (34%), Positives = 52/98 (52%)
Frame = -3
Query: 254 ICWRFIHNRLPTDENLRKRGCTIVSICCFCKKQLETSDHIFLRCPVTSQIWDWLNQGINQ 313
I W+ +HN+ PTD+NL KRG VS+C ET DH FL P T QI +W++ +N
Sbjct: 765 ILWKALHNKFPTDDNLWKRGIHFVSVCTNFGHDSETVDHFFLYRPFTRQI*NWISSMLNC 586
Query: 314 QLDLSSCQNLFVKQNWRRQYVGAANSDLNHQKCHMVHM 351
DLSS N+ + ++ + + HM+++
Sbjct: 585 CSDLSSIHNVLAAITFSFFFIWKRRNLIKF*NQHMIYI 472
>CO982349
Length = 795
Score = 62.0 bits (149), Expect = 5e-10
Identities = 25/77 (32%), Positives = 45/77 (57%)
Frame = +3
Query: 2 RIKSKLSTWHGRLLSIMGRAQLVNYVISGMLVYTFHIHRWPKIILNEITKIIRNFVWSGD 61
+ ++KL+ W R +S+ GR L+N +++ + +Y F R P ++ ++ I R F+W G
Sbjct: 564 KCETKLARWKQRHISLGGRVTLINAILTALPIYFFSFFRAPSKVVQKLVNIQRKFLWGGG 743
Query: 62 CNQQKICTVAWNTMCRP 78
Q++I V W T+C P
Sbjct: 744 SXQRRIAWVKWETVCLP 794
>TC221865
Length = 1045
Score = 60.5 bits (145), Expect = 1e-09
Identities = 36/140 (25%), Positives = 61/140 (42%), Gaps = 9/140 (6%)
Frame = -3
Query: 178 EWNLPTYFHVKDDVLTNKILQTTLPI-KPVPDALQWLSTTDGHLTNKLTYLFVRGTCIKV 236
+W P + + D + + PI + + D W S +GH + + Y ++ K
Sbjct: 761 QWRRPLFDNEVDTTIGFLEDISRFPIHRHLTDCWVWKSEPNGHYSTRSAYHLMQHEAAKA 582
Query: 237 ---PWHKLVWNTYIPPTRVFICWRFIHNRLPTDENLRKRGCTI-VSICCFCKKQLETSDH 292
P + +W IP R + +RLPT NLR+R + ++C FC+ E + H
Sbjct: 581 NTDPAFEEIWKLKIPAKAAIFA*RLVKDRLPTKNNLRRRQVQLNGTLCLFCRNYEEEASH 402
Query: 293 IFLRCPVTSQIW----DWLN 308
+F C T +W W+N
Sbjct: 401 LFFSCTKTQPVWWESLSWIN 342
>BE610670
Length = 368
Score = 60.1 bits (144), Expect = 2e-09
Identities = 33/101 (32%), Positives = 46/101 (44%), Gaps = 4/101 (3%)
Frame = -2
Query: 208 DALQWLSTTDGHLTNKLTYLFVRGTCI---KVPWHKLVWNTYIPPTRVFICWRFIHNRLP 264
D+L W + G + K Y ++G + K++WN IPP + WR NRLP
Sbjct: 313 DSLIWRADPSGAYSTKSAYNLLKGEGSFDNEDSASKIIWNLKIPPRTIAFSWRIFKNRLP 134
Query: 265 TDENLRKRGCTIVSI-CCFCKKQLETSDHIFLRCPVTSQIW 304
T NLR+R + S C C + E HI C T +W
Sbjct: 133 TKANLRRRQVELPSYRCPLCDLEEENVGHIMFSCTRTRSLW 11
>TC233956
Length = 757
Score = 59.7 bits (143), Expect = 2e-09
Identities = 30/101 (29%), Positives = 47/101 (45%), Gaps = 4/101 (3%)
Frame = +2
Query: 208 DALQWLSTTDGHLTNKLTYLFVRGTCIKVPWH---KLVWNTYIPPTRVFICWRFIHNRLP 264
D W + + G ++ K Y ++ H K +W +PP + WR + +RLP
Sbjct: 59 DTWVWRAESTGIISTKSAYQVIKSELDDEGQHLGFKKLWEIKVPPKALSFVWRLLWDRLP 238
Query: 265 TDENLRKRGCTIV-SICCFCKKQLETSDHIFLRCPVTSQIW 304
T +NL KR + +C FC Q ET+ H+F C +W
Sbjct: 239 TKDNLIKRQIQVEDDLCPFCHSQSETASHLFFTCGKIMPLW 361
>TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR retroelement
reverse transcriptase, partial (7%)
Length = 956
Score = 58.2 bits (139), Expect = 7e-09
Identities = 25/41 (60%), Positives = 33/41 (79%)
Frame = -2
Query: 1 DRIKSKLSTWHGRLLSIMGRAQLVNYVISGMLVYTFHIHRW 41
D+IK KL++W G +LSIMGR QLVN VI GML+Y+F ++ W
Sbjct: 175 DKIKCKLASWKGSILSIMGRIQLVNSVIHGMLLYSFSVYAW 53
>BI973267
Length = 406
Score = 58.2 bits (139), Expect = 7e-09
Identities = 31/101 (30%), Positives = 49/101 (47%), Gaps = 4/101 (3%)
Frame = +1
Query: 208 DALQWLSTTDGHLTNKLTYLFVRGTCI---KVPWHKLVWNTYIPPTRVFICWRFIHNRLP 264
D W + +G T K TY ++ + + + N IPP + WR + +RLP
Sbjct: 61 DTWTWRAEANGEFTTKSTYQVLQSELDDQGQYEGFQQLCNIKIPPKALSFAWRLL*DRLP 240
Query: 265 TDENLRKRGCTIVS-ICCFCKKQLETSDHIFLRCPVTSQIW 304
T +NL KR + + +C FC Q ET+ H+F +C +W
Sbjct: 241 TKDNLVKRQIQVENDLCPFCHNQPETASHLFFKCDKIMPMW 363
>CD396188 weakly similar to GP|6175788|gb|A ORF144 {Xestia c-nigrum
granulovirus}, partial (5%)
Length = 701
Score = 58.2 bits (139), Expect = 7e-09
Identities = 26/79 (32%), Positives = 43/79 (53%), Gaps = 1/79 (1%)
Frame = -1
Query: 242 VWNTYIPPTRVFICWRFIHNRLPTDENLRKRGCTIVS-ICCFCKKQLETSDHIFLRCPVT 300
+W IPPT + WR + +RLP+ ENL +R + + +C FC+ Q+E++ H+F C
Sbjct: 626 LWEIKIPPTALSFAWRLLWDRLPSKENLIRRQIVLQNDLCPFCQSQVESASHLFFTCHKV 447
Query: 301 SQIWDWLNQGINQQLDLSS 319
+W N + + L S
Sbjct: 446 MPLWWEFNTWVREDRVLHS 390
>TC231922
Length = 803
Score = 57.8 bits (138), Expect = 9e-09
Identities = 30/101 (29%), Positives = 47/101 (45%), Gaps = 4/101 (3%)
Frame = -3
Query: 208 DALQWLSTTDGHLTNKLTYLFVRGTCIKVPWHKL---VWNTYIPPTRVFICWRFIHNRLP 264
D++ W + + G + K Y + + P ++ +W+ IPP WR +RLP
Sbjct: 444 DSMFWKADSSGVYSTKSAYRLLMPSISPAPSRRIFQILWHLKIPPRAAVFSWRLFLDRLP 265
Query: 265 TDENLRKRGCTIVSICC-FCKKQLETSDHIFLRCPVTSQIW 304
T NL +R I I C C Q E + H+F C +T +W
Sbjct: 264 TRGNLSRRSIPIQDIMCPLCGCQHEEAGHLFFHCKMTKGLW 142
>TC222857
Length = 657
Score = 57.8 bits (138), Expect = 9e-09
Identities = 28/74 (37%), Positives = 40/74 (53%), Gaps = 5/74 (6%)
Frame = -3
Query: 240 KLVWNTYIPPTRVFICWRFIHNRLPTDENLRKRGCTIVSICC-FCKKQLETSDHIFLRCP 298
K +W IP + WR + +RLPT NLR R I + C FC++ ET+ H+F+ C
Sbjct: 619 KDLWKIKIPSKFLMFAWRLLWDRLPTKANLRARQVQISDLTCPFCRRVEETASHMFIHCI 440
Query: 299 VTSQIW----DWLN 308
T IW +W+N
Sbjct: 439 KTQPIWWETMNWIN 398
>CA851073
Length = 633
Score = 57.8 bits (138), Expect = 9e-09
Identities = 35/111 (31%), Positives = 51/111 (45%), Gaps = 5/111 (4%)
Frame = +1
Query: 208 DALQWLSTTDGHLTNKLTYLFVRGTCIK----VPWHKLVWNTYIPPTRVFICWRFIHNRL 263
D + W + G + K Y + TC + K +W IPP WR I +RL
Sbjct: 28 DTMVWKADPCGVYSTKSAYRILM-TCNRHVSEANIFKTIWKLKIPPRAAVFSWRLIKDRL 204
Query: 264 PTDENLRKRGCTI-VSICCFCKKQLETSDHIFLRCPVTSQIWDWLNQGINQ 313
PT NL +R +I + C C + E +DH+F C +T +W W + NQ
Sbjct: 205 PTRHNLLRRNVSIQENECPLCGYEQEEADHLFFNCKMTRGLW-WESMRWNQ 354
>BQ453949
Length = 450
Score = 56.2 bits (134), Expect = 3e-08
Identities = 33/101 (32%), Positives = 47/101 (45%), Gaps = 4/101 (3%)
Frame = +3
Query: 208 DALQWLSTTDGHLTNKLTYLFVRGT---CIKVPWHKLVWNTYIPPTRVFICWRFIHNRLP 264
D L W S +DG + K Y F++ I+ K +WN +PP R NR+P
Sbjct: 30 DLLLWGSNSDGSYSTKSAYNFLKNEDSQTIEDSAFKNIWNLKLPPRACAFS*RIF*NRIP 209
Query: 265 TDENLRKRGCTIVSI-CCFCKKQLETSDHIFLRCPVTSQIW 304
T NLR+R + S C C ++ ET H+ C T +W
Sbjct: 210 TKVNLRRRHVKLPSSNCPLCDEEEETVGHVMYSCIKTRHLW 332
>BI702137
Length = 427
Score = 55.5 bits (132), Expect = 4e-08
Identities = 24/79 (30%), Positives = 41/79 (51%), Gaps = 1/79 (1%)
Frame = +2
Query: 242 VWNTYIPPTRVFICWRFIHNRLPTDENLRKRGCTIVSICC-FCKKQLETSDHIFLRCPVT 300
+W IPP + WR + +RLP+ +NL R + ++ C FC+ Q+E++ H+F C
Sbjct: 68 LWEIKIPPAALSFAWRLLWDRLPSKDNLISRQIVLQNVLCPFCQSQVESASHLFFTCYKV 247
Query: 301 SQIWDWLNQGINQQLDLSS 319
+W N + + L S
Sbjct: 248 MPLWWEFNSWVKEDRVLHS 304
>CF921567
Length = 686
Score = 55.1 bits (131), Expect = 6e-08
Identities = 25/74 (33%), Positives = 40/74 (53%), Gaps = 5/74 (6%)
Frame = -1
Query: 241 LVWNTYIPPTRVFICWRFIHNRLPTDENLRKRGCTIVSICC-FCKKQLETSDHIFLRCPV 299
++W+ IPP WR NRLPT ENL++R ++ + C C + E+ +H+F C
Sbjct: 596 IMWSLKIPPRASAFSWRLFKNRLPTRENLKRRQVSLHTYSCPLCDLEEESVNHLFFNCSK 417
Query: 300 TSQIW----DWLNQ 309
T +W W+N+
Sbjct: 416 TRSLWWEPLRWVNR 375
>TC233392
Length = 1145
Score = 53.5 bits (127), Expect = 2e-07
Identities = 28/78 (35%), Positives = 40/78 (50%), Gaps = 1/78 (1%)
Frame = -1
Query: 242 VWNTYIPPTRVFICWRFIHNRLPTDENLRKRGCTIV-SICCFCKKQLETSDHIFLRCPVT 300
+W IP WR I +RLPT NL +R + S+C FC+ + E + HIFL C
Sbjct: 545 LWKLKIPAKASIFAWRLIRDRLPTKSNLHRRQVVLEDSLCPFCRIREEDASHIFLECNKI 366
Query: 301 SQIWDWLNQGINQQLDLS 318
+W W +Q + L +S
Sbjct: 365 RPLW-WESQTWVRSLGVS 315
>BG404993
Length = 412
Score = 53.1 bits (126), Expect = 2e-07
Identities = 33/102 (32%), Positives = 46/102 (44%), Gaps = 5/102 (4%)
Frame = -3
Query: 208 DALQWLSTTDGHLTNKLTYLF----VRGTCIKVPWHKLVWNTYIPPTRVFICWRFIHNRL 263
D+ W + G+ + K Y +RG + +L W IPP WR I++RL
Sbjct: 332 DSWTWKADPSGNYSTKTAYELLQEAIRGDNEDGIFVQL-WKLKIPPKAPVFTWRLINDRL 156
Query: 264 PTDENLRKRGCTIV-SICCFCKKQLETSDHIFLRCPVTSQIW 304
PT NLR+R I S+C C E + H+F C T W
Sbjct: 155 PTKVNLRRRQVEISDSLCPLCNNSEEDAAHLFFNCNKTLPFW 30
>BQ629929
Length = 437
Score = 53.1 bits (126), Expect = 2e-07
Identities = 23/73 (31%), Positives = 39/73 (52%), Gaps = 1/73 (1%)
Frame = +3
Query: 242 VWNTYIPPTRVFICWRFIHNRLPTDENLRKRGCTIVS-ICCFCKKQLETSDHIFLRCPVT 300
+W +PP + WR + +RLPT ENL +R I + +C FC+ Q+E++ H+F
Sbjct: 18 LWEIKVPPRALSFAWRLLWDRLPTKENLIRRQVVIENDLCPFCQSQVESASHLFFTYKKV 197
Query: 301 SQIWDWLNQGINQ 313
+W N + +
Sbjct: 198 MPMW*EFNSWVKE 236
>CO984521
Length = 716
Score = 52.8 bits (125), Expect = 3e-07
Identities = 31/115 (26%), Positives = 50/115 (42%), Gaps = 9/115 (7%)
Frame = -2
Query: 203 IKP-VPDALQWLSTTDGHLTNKLTYLFVRGTCIKVPWH---KLVWNTYIPPTRVFICWRF 258
I+P + D +W + G + K Y + + K +W +P WR
Sbjct: 664 IRPELSDQWKWAAEPSGCYSTKSAYKALHHVTVGEEQDGKFKELWKLRVPLKVAIFAWRL 485
Query: 259 IHNRLPTDENLRKRGCTIVS-ICCFCKKQLETSDHIFLRCPVTSQIW----DWLN 308
I ++LPT NLRK+ + +C C+ ET+ H+F C S +W W+N
Sbjct: 484 IQDKLPTKANLRKKRVELQEYLCPLCRSVEETASHLFFHCSKVSPLWWESQSWVN 320
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.333 0.142 0.502
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,588,207
Number of Sequences: 63676
Number of extensions: 472967
Number of successful extensions: 4041
Number of sequences better than 10.0: 113
Number of HSP's better than 10.0 without gapping: 3961
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4008
length of query: 408
length of database: 12,639,632
effective HSP length: 100
effective length of query: 308
effective length of database: 6,272,032
effective search space: 1931785856
effective search space used: 1931785856
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.5 bits)
S2: 60 (27.7 bits)
Medicago: description of AC127021.6