BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126786.13 - phase: 0
(916 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC223100 similar to UP|GR33_ARATH (Q9C8E7) Glutamate receptor 3.... 335 7e-92
TC231111 weakly similar to UP|Q7XAV6 (Q7XAV6) Glutamate receptor... 280 3e-75
BM955022 182 6e-46
BE802793 134 2e-31
BI974058 105 1e-22
AI759920 86 1e-16
TC230085 similar to UP|GR36_ARATH (Q84W41) Glutamate receptor 3.... 76 8e-14
TC221804 similar to UP|GR34_ARATH (Q8GXJ4) Glutamate receptor 3.... 69 9e-12
TC229764 weakly similar to UP|GR36_ARATH (Q84W41) Glutamate rece... 61 3e-09
CF921916 58 2e-08
BF008945 weakly similar to PIR|T51136|T511 ionotropic glutamate ... 54 2e-07
TC230086 similar to UP|GR36_ARATH (Q84W41) Glutamate receptor 3.... 48 2e-05
AI966787 36 0.088
AW756252 weakly similar to PIR|T01113|T01 translation initiation... 33 0.57
CD408636 33 0.75
BU545438 32 1.3
TC228324 UP|LGUL_SOYBN (P46417) Lactoylglutathione lyase (Methy... 30 4.8
TC221772 homologue to UP|Q75I55 (Q75I55) Expressed protein, part... 30 6.3
TC233587 29 8.2
>TC223100 similar to UP|GR33_ARATH (Q9C8E7) Glutamate receptor 3.3 precursor
(Ligand-gated ion channel 3.3), partial (25%)
Length = 702
Score = 335 bits (858), Expect = 7e-92
Identities = 156/217 (71%), Positives = 176/217 (80%)
Frame = +2
Query: 421 IVSPETLYANPPNRSSANQHLHTVIWPGETTSRPRGWVFPNNGKQLRIGVPIRASYREFV 480
IV PE LY+ PPNRSSA+Q L V+WPGETT RPRGWVFPNNG+ L+IGVP R SYREFV
Sbjct: 2 IVPPEALYSKPPNRSSASQKLLPVLWPGETTHRPRGWVFPNNGRMLKIGVPKRVSYREFV 181
Query: 481 SPVKGTDLFKGFCVDVFVAAVNLLPYAVPYRFVPFGDGHKNPSYTEFVNKITTGYFDGAV 540
S V+GTD+FKGFC+DVF++AVNLLPYAVPY+FV +GDG NPS TE V IT G FD AV
Sbjct: 182 SQVQGTDMFKGFCIDVFLSAVNLLPYAVPYKFVSYGDGDSNPSNTELVRLITAGVFDAAV 361
Query: 541 GDIAIVTNRTRIVDFTQPYAASGLVVVAPFKKINSGGWSFLQPFTPFMWIVTACFFFFVG 600
GDI I T RT++VDFTQPY SGLVVVA KK +S W+FL PFTP MW VTA FF VG
Sbjct: 362 GDITITTERTKMVDFTQPYIESGLVVVASVKKTDSNAWAFLTPFTPMMWTVTAVFFLLVG 541
Query: 601 IVVWILEHRVNDEFRGSPKQQFVTILWFSLSTLFFSH 637
VVWILEHR+ND+FRG PKQQ VTILWFS ST+FF+H
Sbjct: 542 AVVWILEHRLNDDFRGPPKQQMVTILWFSFSTMFFAH 652
>TC231111 weakly similar to UP|Q7XAV6 (Q7XAV6) Glutamate receptor, partial
(29%)
Length = 940
Score = 280 bits (715), Expect = 3e-75
Identities = 137/259 (52%), Positives = 180/259 (68%)
Frame = +2
Query: 339 SCTNYTSLHSDKAGGLNLDAMSIFDNGTLLLNNILRSNFVGLSGPIKLDSERSLFRPAYD 398
S +N T+L + L+ A+S+ + G LL+NIL N GL+GPI+ RS P+YD
Sbjct: 2 SFSNNTNLSRTREEALDFGALSVSNGGK*LLDNILCINMSGLTGPIQFGLHRSPLNPSYD 181
Query: 399 IINVVGNGVRRVGYWSNYSGLSIVSPETLYANPPNRSSANQHLHTVIWPGETTSRPRGWV 458
I+NV+ G RR+GYWS+YS LS+++PE L+A P NRS ++QHL+ VIWPG TT +P GWV
Sbjct: 182 ILNVIATGYRRIGYWSSYSDLSLINPEKLHAEPANRSISSQHLNCVIWPGNTTEKPHGWV 361
Query: 459 FPNNGKQLRIGVPIRASYREFVSPVKGTDLFKGFCVDVFVAAVNLLPYAVPYRFVPFGDG 518
FPNNG+ LRIG+P R SY + +S + GT+ +G+C+D+F+AA LLPYAV Y+F+ FGDG
Sbjct: 362 FPNNGR*LRIGIPNRVSY*DMISQINGTNAIQGYCIDIFLAAFKLLPYAVQYKFILFGDG 541
Query: 519 HKNPSYTEFVNKITTGYFDGAVGDIAIVTNRTRIVDFTQPYAASGLVVVAPFKKINSGGW 578
HKNPSY + V IT+ FD AVGDIAIV+ T+IVDFT+PY SGLVVVAP KKI
Sbjct: 542 HKNPSYCDLVKMITSDVFDTAVGDIAIVSV*TKIVDFTRPYIESGLVVVAPVKKIEVKCL 721
Query: 579 SFLQPFTPFMWIVTACFFF 597
FL ++ C FF
Sbjct: 722 GFLATIYS-TYVGCHCIFF 775
Score = 94.7 bits (234), Expect = 2e-19
Identities = 40/57 (70%), Positives = 46/57 (80%)
Frame = +3
Query: 571 KKINSGGWSFLQPFTPFMWIVTACFFFFVGIVVWILEHRVNDEFRGSPKQQFVTILW 627
KK+ S W+FL+PFTP MW VTA FF FVG VVWILEHR NDEFRGSP++ VT+LW
Sbjct: 699 KKLKSNAWAFLRPFTPHMWGVTAFFFLFVGAVVWILEHRTNDEFRGSPREHIVTVLW 869
>BM955022
Length = 421
Score = 182 bits (462), Expect = 6e-46
Identities = 86/141 (60%), Positives = 113/141 (79%)
Frame = +1
Query: 108 SHIVAHVANELRVPMLSFAATDPTLSSLQFPFFVRTTLSDLYQMTAVAEIIDFYGWKEVI 167
+H++ H+ANEL+VP+LSF+A DPTLSSLQFPFF+RT SDLYQMTA+A+I++++ WK+VI
Sbjct: 4 AHVITHIANELQVPLLSFSALDPTLSSLQFPFFIRTCHSDLYQMTAIADIVNYHDWKDVI 183
Query: 168 TIYVDDDYGRNGVSALDDALAERRCRISYKVGIKSGPDVDRGEITNLLVNVAMMQSRIIV 227
+Y+DDD GRNG+ AL D LAERRCRISYK + PD EI+N+LV VA+ +SR+IV
Sbjct: 184 AVYLDDDNGRNGIGALGDKLAERRCRISYKAPL--SPDASMEEISNVLVQVALAESRVIV 357
Query: 228 VHAHSNSGFMIFKVAHYLGMM 248
VHA++ G +F VA LGMM
Sbjct: 358 VHANTQFGPKLFSVAKNLGMM 420
>BE802793
Length = 338
Score = 134 bits (336), Expect = 2e-31
Identities = 62/95 (65%), Positives = 75/95 (78%)
Frame = +1
Query: 540 VGDIAIVTNRTRIVDFTQPYAASGLVVVAPFKKINSGGWSFLQPFTPFMWIVTACFFFFV 599
VGDIAIVTNRT IVDF+QP+A+S LV+VAP K S W FLQPFT MW TA F V
Sbjct: 7 VGDIAIVTNRTMIVDFSQPFASSSLVIVAPINKARSNAWVFLQPFTADMWCATAASFLVV 186
Query: 600 GIVVWILEHRVNDEFRGSPKQQFVTILWFSLSTLF 634
G+V+WILEHRVN++FRG PK+Q +T++ FSLS+LF
Sbjct: 187 GVVIWILEHRVNNDFRGPPKKQLLTMVMFSLSSLF 291
>BI974058
Length = 413
Score = 105 bits (261), Expect = 1e-22
Identities = 60/134 (44%), Positives = 84/134 (61%), Gaps = 3/134 (2%)
Frame = +3
Query: 260 LSTVLDSTSLPLE--TMDTLQGALVLRQHTPDTDRKKMFTSKWNNL-TGGSLGLNSYGLH 316
L++ LDS P++ TM+ LQG LVLR HTPDT+ KK F S+ L T + NSY L+
Sbjct: 15 LASTLDSLE-PVDPNTMNLLQGILVLRHHTPDTNEKKSFLSRLKRLKTKETPSFNSYALY 191
Query: 317 AYDTVWLVAQAIDNFFSQGGVVSCTNYTSLHSDKAGGLNLDAMSIFDNGTLLLNNILRSN 376
AYDTV LVA+A+D F +G VVS ++ L L+L ++ +F++G L IL +N
Sbjct: 192 AYDTVCLVARALDAFLKKGSVVSFSSDPKLQDTNGSMLHLQSLRVFNDGPTFLETILSTN 371
Query: 377 FVGLSGPIKLDSER 390
F GL+G ++ D ER
Sbjct: 372 FTGLTGTVQFDIER 413
>AI759920
Length = 139
Score = 85.5 bits (210), Expect = 1e-16
Identities = 35/46 (76%), Positives = 41/46 (89%)
Frame = +2
Query: 592 TACFFFFVGIVVWILEHRVNDEFRGSPKQQFVTILWFSLSTLFFSH 637
TACFF F+GIV+WILEHR+NDEFRG P+QQ +T+LWFSLSTL F H
Sbjct: 2 TACFFLFIGIVIWILEHRINDEFRGPPRQQIITMLWFSLSTLVFFH 139
>TC230085 similar to UP|GR36_ARATH (Q84W41) Glutamate receptor 3.6 precursor
(Ligand-gated ion channel 3.6), partial (14%)
Length = 522
Score = 75.9 bits (185), Expect = 8e-14
Identities = 50/123 (40%), Positives = 66/123 (53%)
Frame = +3
Query: 654 HMVVCGFDN*FQLHC*FNIHTHGAAVIFTYQWH*KLKS***TNRVSSGFIC*TLLD*RYW 713
+MV+C +N* +LH +I+ H A FT H*K * + +GFI L +*R W
Sbjct: 3 YMVICSSNN*LKLHSKLDINPHSATTFFTC*RH*KFNK*QRAYWLLAGFIYANLFN*RNW 182
Query: 714 NIQI*TCSSWISRRICQGTPAWS**RRCCCYC**TALCRNILINPMHIQDCRSGVHQKWL 773
I T SS +RR + T S CCC C** L R + P+ + CRS V+QKWL
Sbjct: 183 Y**IQTSSSENTRRNYRST*ERSPEGWCCCIC**ACLHRALPFKPV*L*HCRSRVYQKWL 362
Query: 774 GVC 776
G+C
Sbjct: 363 GIC 371
>TC221804 similar to UP|GR34_ARATH (Q8GXJ4) Glutamate receptor 3.4 precursor
(Ligand-gated ion channel 3.4) (AtGLR4), partial (20%)
Length = 966
Score = 68.9 bits (167), Expect = 9e-12
Identities = 68/198 (34%), Positives = 99/198 (49%), Gaps = 7/198 (3%)
Frame = +3
Query: 657 VCGFDN*FQLHC*FNIHTHGAAVIFTYQWH*KLKS***TNRVSSGFIC*TLLD*RYWNIQ 716
+CG D+ F+LH * +I+ H AA + T +*+L T+ S I + D +
Sbjct: 3 LCGVDHKFKLHS*LDIYPHCAATVITNCRN*QLDLEYSTDWNSRWVIRAQVSDR*PQHS* 182
Query: 717 I*TCSSWISRRI----CQG--TPAWS**RRCCCYC**TALCRNILI-NPMHIQDCRSGVH 769
I* S+I + C T +W R C C** +LC + + + + +C +GVH
Sbjct: 183 I*--DSYIEKHGGLY*CPSART*SW----RGCSNC**ASLC*GFDV*H*L*VHNCWAGVH 344
Query: 770 QKWLGVCIPSRLPFGGGFVNCHPTTF*NWGSATDS**VDDAKHL*FR*Y*N*IRSSSAQK 829
+KWLG+CIP L V CHP+T * WG A DS* + K + R*Y * + +S K
Sbjct: 345 KKWLGICIPKGLSPCH*LVYCHPSTL*EWGPAKDS*QMA**KGVFNR*Y--*FKQTSFDK 518
Query: 830 FLGSLHHLRGGLLYCSRH 847
LG L + + +C H
Sbjct: 519 LLGPLSDMWHCMCHCPDH 572
>TC229764 weakly similar to UP|GR36_ARATH (Q84W41) Glutamate receptor 3.6
precursor (Ligand-gated ion channel 3.6), partial (18%)
Length = 914
Score = 60.8 bits (146), Expect = 3e-09
Identities = 53/155 (34%), Positives = 66/155 (42%)
Frame = +1
Query: 698 VSSGFIC*TLLD*RYWNIQI*TCSSWISRRICQGTPAWS**RRCCCYC**TALCRNILIN 757
V +GF C* L W + CSS +RR C T S CCC ** L ++
Sbjct: 43 VHTGFFC*KLFSS*NWY**VQACSSNNNRRSC*STEKGSRKWGCCCLH**ACLH*HLPFQ 222
Query: 758 PMHIQDCRSGVHQKWLGVCIPSRLPFGGGFVNCHPTTF*NWGSATDS**VDDAKHL*FR* 817
P+ CRS VHQK LG+C+ + L VNCH W D * + L
Sbjct: 223 PL*FNCCRSRVHQKRLGICLSTGLAISS*PVNCHIANDR*WRFTKDP*QMAFE*RLLITR 402
Query: 818 Y*N*IRSSSAQKFLGSLHHLRGGLLYCSRHIFFAD 852
* + K LG L LR LL C+ I + D
Sbjct: 403 CKA*SGKTPT*KLLGPLCDLRACLLACTLGISYTD 507
>CF921916
Length = 621
Score = 57.8 bits (138), Expect = 2e-08
Identities = 53/188 (28%), Positives = 92/188 (48%), Gaps = 15/188 (7%)
Frame = +2
Query: 96 VIAILGPQSSVVSHIVAHVANEL-----RVPMLSFAATDPT----LSSLQFPFFVRTTLS 146
V+AI+G ++H A +A+EL +VP LS T PT L S Q P F++
Sbjct: 62 VLAIIG----TITHNEATLASELNYTINKVPTLSL--TSPTARTKLLSPQLPHFIQIGDD 223
Query: 147 DLYQMTAVAEIIDFYGWKEVITIYVDDDYGRNGVSALDD---ALAERRCRISYKVGIKSG 203
M VA I+ + WK+V IY +++ + L D AL + I + + S
Sbjct: 224 VRIHMQCVAAIVGEFRWKKVTVIYELNNWLSSDPGMLLDLTYALRQVGSEIDNHLALPSL 403
Query: 204 PDVD--RGEITNLLVNVAMMQSRI-IVVHAHSNSGFMIFKVAHYLGMMQEGYVWIATDWL 260
+ + I N L + +R+ ++VH+ ++F+ A +G+M++G VW+ +D +
Sbjct: 404 SSLSDPKSNIENELKKLKSKSNRVFLIVHSSLELANILFEKAKQIGLMEKGSVWVISDGV 583
Query: 261 STVLDSTS 268
+LDS +
Sbjct: 584 VGLLDSVN 607
>BF008945 weakly similar to PIR|T51136|T511 ionotropic glutamate receptor
glr5 [imported] - Arabidopsis thaliana, partial (5%)
Length = 152
Score = 54.3 bits (129), Expect = 2e-07
Identities = 25/50 (50%), Positives = 33/50 (66%)
Frame = +2
Query: 375 SNFVGLSGPIKLDSERSLFRPAYDIINVVGNGVRRVGYWSNYSGLSIVSP 424
SNF G+SG + +S+RS+ YDIINV G+ VG+WSN S S+V P
Sbjct: 2 SNFTGVSGQLHFNSDRSIVSGGYDIINVNQMGISGVGFWSNNSXFSVVPP 151
>TC230086 similar to UP|GR36_ARATH (Q84W41) Glutamate receptor 3.6 precursor
(Ligand-gated ion channel 3.6), partial (11%)
Length = 836
Score = 48.1 bits (113), Expect = 2e-05
Identities = 34/109 (31%), Positives = 51/109 (46%)
Frame = +2
Query: 745 C**TALCRNILINPMHIQDCRSGVHQKWLGVCIPSRLPFGGGFVNCHPTTF*NWGSATDS 804
C* L R + P+ + CRS V+QKWLG+C+ +RL NC+ W A D
Sbjct: 2 C*RACLHRALPFKPV*L*HCRSRVYQKWLGICLSARLAISS*PFNCNIGVGREWRLAKDP 181
Query: 805 **VDDAKHL*FR*Y*N*IRSSSAQKFLGSLHHLRGGLLYCSRHIFFADY 853
* + + L * + +K LG L + + CS HI ++D+
Sbjct: 182 *QMVFEQCLPITRCKA*SGQTQPEKLLGPLSGVWISMCACSPHILYSDH 328
>AI966787
Length = 145
Score = 35.8 bits (81), Expect = 0.088
Identities = 17/41 (41%), Positives = 22/41 (53%)
Frame = +1
Query: 642 EHNEYSWPRCSTHMVVCGFDN*FQLHC*FNIHTHGAAVIFT 682
EH+E + HM CG + F+LH * NIH H A + T
Sbjct: 1 EHSERPREIGANHMDFCGVNYQFKLHS*LNIHPHSATTVIT 123
>AW756252 weakly similar to PIR|T01113|T01 translation initiation factor
eIF-2C homolog T21L14.12 - Arabidopsis thaliana, partial
(14%)
Length = 435
Score = 33.1 bits (74), Expect = 0.57
Identities = 11/31 (35%), Positives = 21/31 (67%)
Frame = -2
Query: 12 FLFMLPYLEQVYSNSRPSFVNIGAIFTFDSS 42
F F PY+ Q++ + + +N G++F+FD+S
Sbjct: 344 FFFQGPYISQIFLSGKTQRINFGSLFSFDAS 252
>CD408636
Length = 378
Score = 32.7 bits (73), Expect = 0.75
Identities = 18/43 (41%), Positives = 24/43 (54%)
Frame = -1
Query: 594 CFFFFVGIVVWILEHRVNDEFRGSPKQQFVTILWFSLSTLFFS 636
CFFFF GIV+++ HR + SP F + L F L +F S
Sbjct: 309 CFFFFFGIVIYLYFHRFGLHVKCSPSNFFGSSLPF-LGVVFVS 184
>BU545438
Length = 579
Score = 32.0 bits (71), Expect = 1.3
Identities = 19/62 (30%), Positives = 27/62 (42%)
Frame = +1
Query: 590 IVTACFFFFVGIVVWILEHRVNDEFRGSPKQQFVTILWFSLSTLFFSHICRREHNEYSWP 649
++ C+ F ++ WIL H FV + FS LFFSH+ + YS
Sbjct: 406 VILDCYVFCSVVINWILRHC---------NC*FVVAVDFSRLILFFSHVFKYPTKXYSLT 558
Query: 650 RC 651
RC
Sbjct: 559 RC 564
>TC228324 UP|LGUL_SOYBN (P46417) Lactoylglutathione lyase (Methylglyoxalase)
(Aldoketomutase) (Glyoxalase I) , complete
Length = 995
Score = 30.0 bits (66), Expect = 4.8
Identities = 16/42 (38%), Positives = 25/42 (59%)
Frame = -3
Query: 752 RNILINPMHIQDCRSGVHQKWLGVCIPSRLPFGGGFVNCHPT 793
RN+L+N +H+Q R+ VH+ V IP+ L F C+P+
Sbjct: 234 RNLLVNWVHLQKQRTPVHEILFLVFIPNTL-----FSQCNPS 124
>TC221772 homologue to UP|Q75I55 (Q75I55) Expressed protein, partial (14%)
Length = 770
Score = 29.6 bits (65), Expect = 6.3
Identities = 13/30 (43%), Positives = 18/30 (59%)
Frame = +3
Query: 716 QI*TCSSWISRRICQGTPAWS**RRCCCYC 745
Q+ SS++++R C S * RCCCYC
Sbjct: 543 QLGALSSFLAKRACSLVSWESQ*LRCCCYC 632
>TC233587
Length = 703
Score = 29.3 bits (64), Expect = 8.2
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = -2
Query: 584 FTPFMWIVTACFFFFVGIVVWILEHRVNDEFRG 616
+ P WI C F FVGI+ + H ND F G
Sbjct: 120 YCP*SWICLFCRFTFVGILSKNILHNKNDTFGG 22
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.338 0.147 0.498
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 47,212,164
Number of Sequences: 63676
Number of extensions: 786477
Number of successful extensions: 6531
Number of sequences better than 10.0: 39
Number of HSP's better than 10.0 without gapping: 6423
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6521
length of query: 916
length of database: 12,639,632
effective HSP length: 106
effective length of query: 810
effective length of database: 5,889,976
effective search space: 4770880560
effective search space used: 4770880560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.8 bits)
S2: 63 (28.9 bits)
Medicago: description of AC126786.13