BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126019.1 + phase: 0
(253 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC217285 similar to UP|Q8H9B4 (Q8H9B4) UDP-glucose:sterol 3-O-gl... 399 e-112
TC229742 weakly similar to UP|Q8H9B4 (Q8H9B4) UDP-glucose:sterol... 283 5e-77
TC231010 similar to UP|Q8H9B4 (Q8H9B4) UDP-glucose:sterol 3-O-gl... 255 2e-68
TC217286 weakly similar to UP|Q8H9B4 (Q8H9B4) UDP-glucose:sterol... 80 1e-15
BI968651 55 2e-08
BG047022 44 9e-05
TC208101 weakly similar to UP|Q9ZWS2 (Q9ZWS2) Flavonoid 3-O-gala... 40 0.001
TC230658 similar to UP|Q9FT43 (Q9FT43) Sterol glucosyltransferas... 32 0.36
TC215055 similar to UP|Q9XH69 (Q9XH69) Beta-amylase (Fragment), ... 31 0.47
TC216848 similar to UP|Q6VAB3 (Q6VAB3) UDP-glycosyltransferase 8... 30 0.81
TC227247 UP|Q6T2Z8 (Q6T2Z8) Cyclin-dependent kinases CDKB, complete 30 1.4
TC216849 weakly similar to UP|Q6VAB3 (Q6VAB3) UDP-glycosyltransf... 29 1.8
TC209756 28 3.1
CO984858 28 4.0
TC217763 weakly similar to GB|AAB68983.1|500656|YSCH9666 Yhr143w... 28 4.0
TC214915 similar to UP|Q42487 (Q42487) Extensin like protein, pa... 28 4.0
CO983694 28 5.2
CA853840 similar to GP|6708183|gb| papain-type cysteine endopept... 27 6.8
CA802771 27 6.8
TC221283 27 8.9
>TC217285 similar to UP|Q8H9B4 (Q8H9B4) UDP-glucose:sterol
3-O-glucosyltransferase, partial (59%)
Length = 1302
Score = 399 bits (1025), Expect = e-112
Identities = 188/226 (83%), Positives = 202/226 (89%)
Frame = +2
Query: 28 DWGPKIDVVGFCFLDLASNYEPPESLVKWLEDGDKPIYVGFGSLPVQDPKTMTEIIVEAL 87
DWGPKIDVVGFCFLDLA NYEPPESLVKWLE+GDKPIY+GFGSLPVQ+PK MT+IIV+AL
Sbjct: 419 DWGPKIDVVGFCFLDLALNYEPPESLVKWLEEGDKPIYIGFGSLPVQEPKKMTQIIVDAL 598
Query: 88 ETTGQRGIISKGWGGLGDLTEPKDSIYLLDNVPHDWLFLHCKAVVHHGGAGTTAAGLKAA 147
E TGQRGII+KGWGGLG+L EPKDSIYLLDN PHDWLFL CKAVVHHGGAGTTAAGLKAA
Sbjct: 599 EITGQRGIINKGWGGLGNLAEPKDSIYLLDNCPHDWLFLRCKAVVHHGGAGTTAAGLKAA 778
Query: 148 CPTTIVPFFGDQPFWGERVHDRGVGPPPIPIDVFSLPKLIAAINFMLDPKVKEHAIELAK 207
CPTTIVPFFGDQPFWGERVH RGVGPPPIP+D FSLPKL+ AI MLDPKVKE AIELAK
Sbjct: 779 CPTTIVPFFGDQPFWGERVHARGVGPPPIPVDEFSLPKLVDAIKLMLDPKVKERAIELAK 958
Query: 208 AMENEDGVTGAVKVFFKQLPKNKPEPNTEPSSSSCFSYIARCFGYS 253
AMENEDGVTGAVK FFKQLP+ K E + +P + FS + RCFG S
Sbjct: 959 AMENEDGVTGAVKAFFKQLPQKKSESDADPQPTGFFS-VRRCFGCS 1093
>TC229742 weakly similar to UP|Q8H9B4 (Q8H9B4) UDP-glucose:sterol
3-O-glucosyltransferase, partial (34%)
Length = 1133
Score = 283 bits (724), Expect = 5e-77
Identities = 125/207 (60%), Positives = 158/207 (75%)
Frame = +2
Query: 27 ADWGPKIDVVGFCFLDLASNYEPPESLVKWLEDGDKPIYVGFGSLPVQDPKTMTEIIVEA 86
+DWGP +DVVG+CFL+L S Y+P E V+W++ G KP+Y GFGS+P+ DPK T++IVEA
Sbjct: 164 SDWGPLVDVVGYCFLNLGSKYQPQEDFVRWIQKGPKPLYFGFGSMPLDDPKRTTDVIVEA 343
Query: 87 LETTGQRGIISKGWGGLGDLTEPKDSIYLLDNVPHDWLFLHCKAVVHHGGAGTTAAGLKA 146
L+ TGQRGII +GWG LG+L E D++++L+ PHDWLF C A+VHHGGAGTTA GLKA
Sbjct: 344 LKDTGQRGIIDRGWGNLGNLAEVPDNVFVLEECPHDWLFPQCSALVHHGGAGTTATGLKA 523
Query: 147 ACPTTIVPFFGDQPFWGERVHDRGVGPPPIPIDVFSLPKLIAAINFMLDPKVKEHAIELA 206
CPTTIVPFFGDQ FWG+R++ +G+GP PIPI SL L +I FML P+VK A+E+A
Sbjct: 524 GCPTTIVPFFGDQFFWGDRINQKGLGPAPIPISQLSLENLSNSIKFMLQPEVKSRAMEVA 703
Query: 207 KAMENEDGVTGAVKVFFKQLPKNKPEP 233
K +ENEDGVT AV F + LP P P
Sbjct: 704 KLIENEDGVTAAVDSFHRHLPPELPLP 784
>TC231010 similar to UP|Q8H9B4 (Q8H9B4) UDP-glucose:sterol
3-O-glucosyltransferase, partial (28%)
Length = 893
Score = 255 bits (651), Expect = 2e-68
Identities = 122/190 (64%), Positives = 144/190 (75%), Gaps = 2/190 (1%)
Frame = +1
Query: 66 VGFGSLPVQDPKTMTEIIVEALETTGQRGIISKGWGGLGDLTEPKDSIYLLDNVPHDWLF 125
VGFGSLP+Q P+ +T+II++ALE TGQRGII+KGWGGLG L E S+YLLDN PHDWLF
Sbjct: 1 VGFGSLPLQQPEKITQIIIQALEETGQRGIINKGWGGLGSLAEQNKSVYLLDNCPHDWLF 180
Query: 126 LHCKAVVHHGGAGTTAAGLKAACPTTIVPFFGDQPFWGERVHDRGVGPPPIPIDVFSLPK 185
C AVVHHGGAGTTAAGL+A CPTTIVPFFGDQPFWG+RV RGVGP PIP+D FS +
Sbjct: 181 PRCTAVVHHGGAGTTAAGLRAECPTTIVPFFGDQPFWGDRVRARGVGPAPIPVDEFSFDR 360
Query: 186 LIAAINFMLDPKVKEHAIELAKAMENEDGVTGAVKVFFKQLPK--NKPEPNTEPSSSSCF 243
L+ AI+ ML P+VK+ A+ELA AM+NE+GV GAVK F+K P + EP S +
Sbjct: 361 LVDAIHLMLKPEVKKRAVELANAMKNENGVLGAVKAFYKHYPAEFKRLASTAEPKSVHKY 540
Query: 244 SYIARCFGYS 253
I CFG S
Sbjct: 541 FSIRGCFGCS 570
>TC217286 weakly similar to UP|Q8H9B4 (Q8H9B4) UDP-glucose:sterol
3-O-glucosyltransferase, partial (7%)
Length = 436
Score = 79.7 bits (195), Expect = 1e-15
Identities = 39/54 (72%), Positives = 44/54 (81%)
Frame = +2
Query: 200 EHAIELAKAMENEDGVTGAVKVFFKQLPKNKPEPNTEPSSSSCFSYIARCFGYS 253
E AIELAKAMENEDGVTGAVK FFKQLP+ KPEP+ +P +S FS + RCFG S
Sbjct: 2 ERAIELAKAMENEDGVTGAVKAFFKQLPQKKPEPDADPQPTSFFS-VGRCFGCS 160
>BI968651
Length = 349
Score = 55.5 bits (132), Expect = 2e-08
Identities = 30/59 (50%), Positives = 38/59 (63%)
Frame = -1
Query: 195 DPKVKEHAIELAKAMENEDGVTGAVKVFFKQLPKNKPEPNTEPSSSSCFSYIARCFGYS 253
DPKVK A E+AK+ NED V+G VK F +QLP+ KPEP+T + S + R FG S
Sbjct: 349 DPKVK*RASEVAKSXXNEDXVSGPVKAFGQQLPQKKPEPDTNAQTYRSLS-VGRGFGCS 176
>BG047022
Length = 405
Score = 43.5 bits (101), Expect = 9e-05
Identities = 16/22 (72%), Positives = 20/22 (90%)
Frame = +1
Query: 148 CPTTIVPFFGDQPFWGERVHDR 169
CPTTIVPFFGDQ FW +R++D+
Sbjct: 334 CPTTIVPFFGDQFFWRDRIYDK 399
>TC208101 weakly similar to UP|Q9ZWS2 (Q9ZWS2) Flavonoid 3-O-galactosyl
transferase, partial (46%)
Length = 1022
Score = 39.7 bits (91), Expect = 0.001
Identities = 42/186 (22%), Positives = 74/186 (39%), Gaps = 13/186 (6%)
Frame = +3
Query: 54 VKWL--EDGDKPIYVGFGSLPVQDPKTMTEIIVEALETTG-------QRGIISKGWGGLG 104
+ WL ++ YV FG++ P + + EALE +G + G++S G
Sbjct: 213 LSWLGMKNSKSVAYVCFGTVVAPPPHELVAV-AEALEESGFPFLWSLKEGLMSLLPNGFV 389
Query: 105 DLTEPKDSIYLLDNVPHDWLFLHCKAVVHHGGAGTTAAGLKAACPTTIVPFFGDQPFWGE 164
+ T+ + I H V H GA + + + P PFFGDQ
Sbjct: 390 ERTKKRGKIVSWAPQTHVLAHDSVGVFVTHCGANSVIESVSSGVPMICRPFFGDQGVAAR 569
Query: 165 RVHDRGVGPPPIPIDVFSLPKLIAAINFML----DPKVKEHAIELAKAMENEDGVTGAVK 220
+ D I +F+ L+ ++N +L K++++A+ + K +E+ G
Sbjct: 570 VIEDVWEIGMMIEGKMFTKNGLVKSLNLILVHEEGKKIRDNALRVKKTVEDAGRPEGQAT 749
Query: 221 VFFKQL 226
F L
Sbjct: 750 QDFNTL 767
>TC230658 similar to UP|Q9FT43 (Q9FT43) Sterol glucosyltransferase-like
protein, partial (6%)
Length = 883
Score = 31.6 bits (70), Expect = 0.36
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3
Query: 152 IVPFFGDQPFWGERVHDRGVGPPPI 176
+ PF DQ +W ER+H GV P P+
Sbjct: 732 VCPFILDQFYWAERMHWLGVSPEPL 806
>TC215055 similar to UP|Q9XH69 (Q9XH69) Beta-amylase (Fragment), partial (6%)
Length = 481
Score = 31.2 bits (69), Expect = 0.47
Identities = 25/81 (30%), Positives = 37/81 (44%)
Frame = +2
Query: 66 VGFGSLPVQDPKTMTEIIVEALETTGQRGIISKGWGGLGDLTEPKDSIYLLDNVPHDWLF 125
V FG ++ K + AL+T R K G+G ++ D + L +P D +
Sbjct: 254 VSFGRNNIRWEKAGISFTLRALQTEPVRE--EKKPSGIGTRSKTVDGVRLFVGLPLDAVS 427
Query: 126 LHCKAVVHHGGAGTTAAGLKA 146
CK++ H A AAGLKA
Sbjct: 428 YDCKSINH---ARAIAAGLKA 481
>TC216848 similar to UP|Q6VAB3 (Q6VAB3) UDP-glycosyltransferase 85A8, partial
(39%)
Length = 1113
Score = 30.4 bits (67), Expect = 0.81
Identities = 33/136 (24%), Positives = 51/136 (37%), Gaps = 18/136 (13%)
Frame = +1
Query: 43 LASN-YEPPESLVKWLEDGD--KPIYVGFGSLPVQDPKTMTEI-------------IVEA 86
L SN ++ + WLE + +YV FGS+ V + + E I+
Sbjct: 265 LGSNLWKEDTGCLDWLESKEPRSVVYVNFGSITVMSAEQLLEFAWGLANSKKPFLWIIRP 444
Query: 87 LETTGQRGIISKGWGGLGDLTEPKDSIYLLDNVPHDWLFLHCKAVVH--HGGAGTTAAGL 144
G I+S + + E +D + P + + H V H G +T +
Sbjct: 445 DLVIGGSVILSSEF-----VNETRDRSLIASWCPQEQVLNHPSIGVFLTHCGWNSTTESI 609
Query: 145 KAACPTTIVPFFGDQP 160
A P PFF DQP
Sbjct: 610 CAGVPMLCWPFFADQP 657
>TC227247 UP|Q6T2Z8 (Q6T2Z8) Cyclin-dependent kinases CDKB, complete
Length = 1235
Score = 29.6 bits (65), Expect = 1.4
Identities = 22/62 (35%), Positives = 26/62 (41%), Gaps = 6/62 (9%)
Frame = -2
Query: 71 LPVQDPKTMTEIIVEALETTGQRGIISKGWGGLGDLTEPKDSI------YLLDNVPHDWL 124
+PVQDP T+ E AL + GW GL LTE + L D V H
Sbjct: 472 VPVQDPMTVAES--NALAKLVHQAFDGLGWNGLSSLTERANEFLEIGVHVLKDQVEHCLP 299
Query: 125 FL 126
FL
Sbjct: 298 FL 293
>TC216849 weakly similar to UP|Q6VAB3 (Q6VAB3) UDP-glycosyltransferase 85A8,
partial (47%)
Length = 1125
Score = 29.3 bits (64), Expect = 1.8
Identities = 29/124 (23%), Positives = 48/124 (38%), Gaps = 17/124 (13%)
Frame = +2
Query: 54 VKWLEDGDKP--IYVGFGSLPVQDPKTMTEI-------------IVEALETTGQRGIISK 98
++WLE + +YV FGS+ V + + E I+ G I+S
Sbjct: 338 LEWLESKESRSVVYVNFGSITVMSAEQLLEFAWGLANSKKPFLWIIRPDLVIGGSVILSS 517
Query: 99 GWGGLGDLTEPKDSIYLLDNVPHDWLFLHCKAVVH--HGGAGTTAAGLKAACPTTIVPFF 156
+ ++E +D + P + + H V H G +T + A P PFF
Sbjct: 518 EF-----VSETRDRSLIASWCPQEQVLNHPSIGVFLTHCGWNSTTESVCAGVPMLCWPFF 682
Query: 157 GDQP 160
+QP
Sbjct: 683 AEQP 694
>TC209756
Length = 1356
Score = 28.5 bits (62), Expect = 3.1
Identities = 14/38 (36%), Positives = 20/38 (51%), Gaps = 2/38 (5%)
Frame = +1
Query: 47 YEPPESLVKWLEDG--DKPIYVGFGSLPVQDPKTMTEI 82
+E + ++WL+ IYV FGSL V DP E+
Sbjct: 286 WEEDTTCLEWLDQQPPQSVIYVSFGSLAVMDPNQFKEL 399
>CO984858
Length = 778
Score = 28.1 bits (61), Expect = 4.0
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
Frame = -2
Query: 51 ESLVKWLEDGDKP--IYVGFGSLPVQDPKTMTEI 82
E V+WL+D K +YV FGS+ + M E+
Sbjct: 693 EECVEWLDDKPKGSVVYVSFGSMATMSEEQMEEV 592
>TC217763 weakly similar to GB|AAB68983.1|500656|YSCH9666 Yhr143wp
{Saccharomyces cerevisiae;} , partial (6%)
Length = 985
Score = 28.1 bits (61), Expect = 4.0
Identities = 13/27 (48%), Positives = 15/27 (55%)
Frame = +1
Query: 227 PKNKPEPNTEPSSSSCFSYIARCFGYS 253
P N P+PN SSSS S + CF S
Sbjct: 121 PTNPPKPNPSLSSSSSSSISSHCFNLS 201
>TC214915 similar to UP|Q42487 (Q42487) Extensin like protein, partial (95%)
Length = 1149
Score = 28.1 bits (61), Expect = 4.0
Identities = 11/19 (57%), Positives = 12/19 (62%)
Frame = +3
Query: 224 KQLPKNKPEPNTEPSSSSC 242
K PK KP PN PS +SC
Sbjct: 195 KPKPKPKPNPNPSPSGASC 251
>CO983694
Length = 858
Score = 27.7 bits (60), Expect = 5.2
Identities = 36/142 (25%), Positives = 52/142 (36%), Gaps = 20/142 (14%)
Frame = -1
Query: 47 YEPPESLVKWLEDGDKP----IYVGFGSLPVQDPKTMTEIIVEALETTG----------Q 92
++ S + WL KP IY+ FGS+ + M EI + L TG +
Sbjct: 777 FQVDSSAISWLRQ--KPAGSVIYISFGSMVCFSSQQMEEIAL-GLMATGFNFLWVIPDLE 607
Query: 93 RGIISKGWG------GLGDLTEPKDSIYLLDNVPHDWLFLHCKAVVHHGGAGTTAAGLKA 146
R + K G G G + + +L N F HC G +T L
Sbjct: 606 RKNLPKELGEEINACGRGLIVNWTPQLEVLSNHAVGCFFTHC-------GWNSTLEALCL 448
Query: 147 ACPTTIVPFFGDQPFWGERVHD 168
P +P + DQP + V D
Sbjct: 447 GVPMVALPQWTDQPTNAKFVED 382
>CA853840 similar to GP|6708183|gb| papain-type cysteine endopeptidase XCP2
{Arabidopsis thaliana}, partial (29%)
Length = 459
Score = 27.3 bits (59), Expect = 6.8
Identities = 22/102 (21%), Positives = 43/102 (41%), Gaps = 1/102 (0%)
Frame = +2
Query: 8 FKKLTQVILLSIKYIIFLFADWGPKIDVVGFCFLDLASNYEPPESLVKWLEDGDKPIYVG 67
F ++L++ + +F +G +VG+ DL S + E W+ K IY
Sbjct: 11 FSSSKALVLIACSFCLFASLAFGRDFSIVGYSSEDLKSMDKLIELFESWMSRHGK-IYEN 187
Query: 68 FGSLPVQDPKTMTEIIVEALETTGQRG-IISKGWGGLGDLTE 108
+++ EI + L+ +R ++S W GL + +
Sbjct: 188 -----IEEKLLRFEIFKDNLKHIDERNKVVSNYWLGLNEFAD 298
>CA802771
Length = 456
Score = 27.3 bits (59), Expect = 6.8
Identities = 17/51 (33%), Positives = 20/51 (38%), Gaps = 10/51 (19%)
Frame = +1
Query: 213 DGVTGAVKVFFKQLPKNKPE----------PNTEPSSSSCFSYIARCFGYS 253
DGV G VK F+K P K + EP + I CFG S
Sbjct: 1 DGVLGLVKAFYKHYPPEKSKFDDAKSKQLASTAEPKPVHKYFSIRGCFGCS 153
>TC221283
Length = 360
Score = 26.9 bits (58), Expect = 8.9
Identities = 7/25 (28%), Positives = 19/25 (76%)
Frame = +1
Query: 1 MCNLLYIFKKLTQVILLSIKYIIFL 25
+CN+L++FK L+ +++ Y++++
Sbjct: 271 LCNILFVFKLLSHTAIVNGYYVVYM 345
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.142 0.453
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,154,759
Number of Sequences: 63676
Number of extensions: 210583
Number of successful extensions: 1030
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 1026
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1029
length of query: 253
length of database: 12,639,632
effective HSP length: 95
effective length of query: 158
effective length of database: 6,590,412
effective search space: 1041285096
effective search space used: 1041285096
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC126019.1