BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126018.10 + phase: 0
(72 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC217358 homologue to UP|CLPP_LOTJA (Q9BBQ9) ATP-dependent Clp p... 104 7e-24
TC218115 similar to UP|Q94B60 (Q94B60) ATP-dependent Clp proteas... 50 2e-07
TC208328 similar to GB|AAO63325.1|28950803|BT005261 At5g23140 {A... 50 2e-07
TC206242 homologue to UP|Q9S834 (Q9S834) ATP-dependent Clp prote... 50 2e-07
BE058784 similar to SP|P56772|CLPP_ ATP-dependent Clp protease p... 44 2e-05
TC215901 similar to UP|O82421 (O82421) Clp protease (Fragment) ,... 42 5e-05
TC205544 similar to UP|Q93ZD4 (Q93ZD4) At1g12410/F5O11_7, partia... 42 6e-05
TC208296 similar to UP|O23548 (O23548) CLP proteinase like prote... 40 2e-04
TC205545 similar to PIR|T52454|T52454 ATP-dependent Clp proteina... 39 4e-04
TC234696 similar to UP|O49931 (O49931) Rieske iron-sulfur protei... 25 9.8
TC225925 similar to UP|NUFM_ARATH (Q9FLX7) Probable NADH-ubiquin... 25 9.8
TC205411 similar to UP|C824_SOYBN (O49859) Cytochrome P450 82A4 ... 25 9.8
BI674452 25 9.8
>TC217358 homologue to UP|CLPP_LOTJA (Q9BBQ9) ATP-dependent Clp protease
proteolytic subunit (Endopeptidase Clp) , partial (76%)
Length = 1519
Score = 104 bits (260), Expect = 7e-24
Identities = 51/71 (71%), Positives = 58/71 (80%)
Frame = +1
Query: 1 MIHQPATSLYEGQVGECMLEAEELLKMRKTITNIYAQRTGKASWQIYRDMERDLFMSAEE 60
MIHQPA+S YE Q GE +LEAEELLKMR+TIT +Y QRTGK W I DMERD+FMSA E
Sbjct: 238 MIHQPASSFYEAQTGEFILEAEELLKMRETITRVYVQRTGKPLWVISEDMERDVFMSAAE 417
Query: 61 AEAHGIVDTVA 71
A+A+GIVD VA
Sbjct: 418 AQAYGIVDLVA 450
>TC218115 similar to UP|Q94B60 (Q94B60) ATP-dependent Clp protease-like
protein (At5g45390), partial (77%)
Length = 1087
Score = 50.1 bits (118), Expect = 2e-07
Identities = 26/70 (37%), Positives = 43/70 (61%)
Frame = +3
Query: 1 MIHQPATSLYEGQVGECMLEAEELLKMRKTITNIYAQRTGKASWQIYRDMERDLFMSAEE 60
MIHQP GQ + ++A+E++ + IT I + TG++ Q+ +D++RD +MS E
Sbjct: 615 MIHQPLGGA-SGQAIDVEIQAKEVMHNKNNITRIISSFTGRSFEQVQKDIDRDKYMSPIE 791
Query: 61 AEAHGIVDTV 70
A +GI+D V
Sbjct: 792 AVEYGIIDGV 821
>TC208328 similar to GB|AAO63325.1|28950803|BT005261 At5g23140 {Arabidopsis
thaliana;} , partial (88%)
Length = 1033
Score = 50.1 bits (118), Expect = 2e-07
Identities = 23/70 (32%), Positives = 44/70 (62%)
Frame = +2
Query: 1 MIHQPATSLYEGQVGECMLEAEELLKMRKTITNIYAQRTGKASWQIYRDMERDLFMSAEE 60
MIHQP+ Y GQ + + + ++++ ++ +YA+ TG++ I +M+RD FM+ +E
Sbjct: 479 MIHQPSGG-YSGQAKDIAIHTKHIVRVWDSLNELYAKHTGQSVEVIQTNMDRDNFMTPKE 655
Query: 61 AEAHGIVDTV 70
A+ G++D V
Sbjct: 656 AKEFGLIDEV 685
>TC206242 homologue to UP|Q9S834 (Q9S834) ATP-dependent Clp protease subunit
ClpP (NClpP1) (ATP-dependent Clp protease proteolytic
subunit ClpP5) , partial (86%)
Length = 1276
Score = 50.1 bits (118), Expect = 2e-07
Identities = 26/70 (37%), Positives = 42/70 (59%)
Frame = +1
Query: 1 MIHQPATSLYEGQVGECMLEAEELLKMRKTITNIYAQRTGKASWQIYRDMERDLFMSAEE 60
MIHQP GQ + ++A E+L + + A TG++ +I +D +RD FMSA+E
Sbjct: 745 MIHQPLGGAQGGQT-DIDIQANEMLHHKANLNGYLAYHTGQSLDKINQDTDRDFFMSAKE 921
Query: 61 AEAHGIVDTV 70
A+ +G++D V
Sbjct: 922 AKEYGLIDGV 951
>BE058784 similar to SP|P56772|CLPP_ ATP-dependent Clp protease proteolytic
subunit (EC 3.4.21.92) (Endopeptidase Clp). [Mouse-ear
cress], partial (18%)
Length = 272
Score = 43.9 bits (102), Expect = 2e-05
Identities = 25/42 (59%), Positives = 28/42 (66%), Gaps = 1/42 (2%)
Frame = +2
Query: 31 ITNIYAQRTGKASWQIYRDMERDLFMS-AEEAEAHGIVDTVA 71
I QRT K I DM+RD+FMS AEEA+AHGIVD VA
Sbjct: 137 ILGFMLQRTSKHLCLISEDMQRDVFMSPAEEAQAHGIVDLVA 262
>TC215901 similar to UP|O82421 (O82421) Clp protease (Fragment) , partial
(75%)
Length = 1214
Score = 42.4 bits (98), Expect = 5e-05
Identities = 26/70 (37%), Positives = 38/70 (54%)
Frame = +1
Query: 1 MIHQPATSLYEGQVGECMLEAEELLKMRKTITNIYAQRTGKASWQIYRDMERDLFMSAEE 60
MIHQP + G+ E + E+ + I I ++ TGK QI D +RD FM+ E
Sbjct: 583 MIHQPLGTA-GGKATEMSIRIREMAYHKIKINKILSRITGKPEEQIELDTDRDNFMNPWE 759
Query: 61 AEAHGIVDTV 70
A+ +G+VD V
Sbjct: 760 AKEYGLVDGV 789
>TC205544 similar to UP|Q93ZD4 (Q93ZD4) At1g12410/F5O11_7, partial (53%)
Length = 642
Score = 42.0 bits (97), Expect = 6e-05
Identities = 21/59 (35%), Positives = 36/59 (60%)
Frame = +2
Query: 12 GQVGECMLEAEELLKMRKTITNIYAQRTGKASWQIYRDMERDLFMSAEEAEAHGIVDTV 70
GQ + EA ELL++R + N AQ+TG+ +I +D+ R +A+EA +G++D +
Sbjct: 230 GQADDIQNEANELLRIRDYLFNELAQKTGQPVEKITKDLSRMKRFNAQEALEYGLIDRI 406
>TC208296 similar to UP|O23548 (O23548) CLP proteinase like protein, partial
(74%)
Length = 1003
Score = 40.4 bits (93), Expect = 2e-04
Identities = 22/70 (31%), Positives = 36/70 (51%)
Frame = +3
Query: 1 MIHQPATSLYEGQVGECMLEAEELLKMRKTITNIYAQRTGKASWQIYRDMERDLFMSAEE 60
MI QP ++GQ + L E+ ++ + +YA+ K QI D++R + S E
Sbjct: 474 MIRQPIAR-FQGQATDVNLARREVNNVKTELVKLYAKHMEKTPEQIEADIQRPKYFSPSE 650
Query: 61 AEAHGIVDTV 70
A +GI+D V
Sbjct: 651 AVEYGIIDKV 680
>TC205545 similar to PIR|T52454|T52454 ATP-dependent Clp proteinase
catalytic chain P2 [imported] - Arabidopsis thaliana
{Arabidopsis thaliana;} , partial (81%)
Length = 1369
Score = 39.3 bits (90), Expect = 4e-04
Identities = 19/59 (32%), Positives = 36/59 (60%)
Frame = +3
Query: 12 GQVGECMLEAEELLKMRKTITNIYAQRTGKASWQIYRDMERDLFMSAEEAEAHGIVDTV 70
GQ + EA ELL++R + N +++TG+ +I +D+ R +A+EA +G++D +
Sbjct: 726 GQADDIQNEANELLRIRDYLFNELSKKTGQPLEKITKDLSRMKRFNAQEALEYGLIDRI 902
>TC234696 similar to UP|O49931 (O49931) Rieske iron-sulfur protein Tic55
precursor, partial (34%)
Length = 616
Score = 24.6 bits (52), Expect = 9.8
Identities = 11/43 (25%), Positives = 21/43 (48%)
Frame = +2
Query: 2 IHQPATSLYEGQVGECMLEAEELLKMRKTITNIYAQRTGKASW 44
+HQ A+ ++E +G + E LLK + + +Y +W
Sbjct: 11 LHQNASKVFEQDMGFLSSQNEILLKEKVSTKELYLNLKSSDTW 139
>TC225925 similar to UP|NUFM_ARATH (Q9FLX7) Probable NADH-ubiquinone
oxidoreductase 18 kDa subunit, mitochondrial precursor
(Complex I-18Kd) (CI-18Kd) , partial (88%)
Length = 809
Score = 24.6 bits (52), Expect = 9.8
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +3
Query: 36 AQRTGKASWQIYRDMERD 53
A+R G A W + +D ERD
Sbjct: 186 AEREGGADWAVQQDAERD 239
>TC205411 similar to UP|C824_SOYBN (O49859) Cytochrome P450 82A4 (P450 CP9)
, partial (56%)
Length = 1004
Score = 24.6 bits (52), Expect = 9.8
Identities = 10/47 (21%), Positives = 23/47 (48%)
Frame = +3
Query: 14 VGECMLEAEELLKMRKTITNIYAQRTGKASWQIYRDMERDLFMSAEE 60
+G+C L + K + ITN++ T + W + + + F++ +
Sbjct: 510 IGDCTLGGYNVKKGTRLITNLWKIHTDPSVWSNSLEFKPERFLTTHK 650
>BI674452
Length = 366
Score = 24.6 bits (52), Expect = 9.8
Identities = 12/40 (30%), Positives = 19/40 (47%)
Frame = +3
Query: 16 ECMLEAEELLKMRKTITNIYAQRTGKASWQIYRDMERDLF 55
EC+L ELLK + + ++ Q W I +E +F
Sbjct: 81 ECLLLLPELLKKVERLKSMCLQYAAATQWLISSSIELHIF 200
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.129 0.363
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,430,332
Number of Sequences: 63676
Number of extensions: 18817
Number of successful extensions: 84
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 83
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 83
length of query: 72
length of database: 12,639,632
effective HSP length: 48
effective length of query: 24
effective length of database: 9,583,184
effective search space: 229996416
effective search space used: 229996416
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)
Medicago: description of AC126018.10