BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC125478.7 - phase: 0
(357 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AI495080 89 3e-18
CA820303 75 3e-14
BQ576456 65 6e-11
TC232778 similar to UP|Q9LNG5 (Q9LNG5) F21D18.16, partial (5%) 54 1e-07
TC211858 52 3e-07
CO979307 47 1e-05
BG881451 weakly similar to PIR|D96521|D96 protein F21D18.16 [imp... 38 0.008
BF071225 weakly similar to PIR|D96521|D96 protein F21D18.16 [imp... 34 0.089
TC234144 34 0.12
TC220808 weakly similar to UP|Q9SK32 (Q9SK32) Expressed protein,... 32 0.44
TC232239 28 4.9
TC224671 similar to UP|Q8S8Z0 (Q8S8Z0) Protein phosphatase 2C, p... 28 8.3
BE659518 28 8.3
>AI495080
Length = 407
Score = 89.0 bits (219), Expect = 3e-18
Identities = 50/122 (40%), Positives = 65/122 (52%), Gaps = 3/122 (2%)
Frame = +2
Query: 165 RALGGSVTLLTAWFLAHFPGFFSVDLNTDYLENYSVAARWKLQKGHGEGI---TYRSLLD 221
R L G +TLL W HFP + DY E A RW K + I +YR L+
Sbjct: 2 RQLSGYITLLQCWIYEHFPSVAESTAD*DYDEASPRACRWIATKKTVKSIRTPSYRERLN 181
Query: 222 RIQLDDVCWRSYEEHREIQDFEEVFWYSRWIMCGVRRVYRHLPERVLRQYGYVQTIPRHP 281
R+++ DVCW Y EHRE+QDF YS + G+ VY + PE+V+RQ+GY IP P
Sbjct: 182 RLRISDVCWILYGEHREVQDFHVRSCYSGLLHWGLVAVY-YRPEKVVRQFGYTPAIPAPP 358
Query: 282 TD 283
D
Sbjct: 359 VD 364
>CA820303
Length = 421
Score = 75.5 bits (184), Expect = 3e-14
Identities = 50/139 (35%), Positives = 70/139 (49%), Gaps = 6/139 (4%)
Frame = -1
Query: 215 TYRSLLDRIQLDDVCWRSYEEHREIQDFEEVFWYSRWIMCGVRRVYRHLPERVLRQYGYV 274
+YR LDR+++ DVCW Y EHRE+QDF YS + G VY + ERV+RQ+GY
Sbjct: 400 SYRERLDRLRIPDVCWIPYGEHREVQDFHVRSCYSGLLCWGPVVVY-YRSERVVRQFGYT 224
Query: 275 QTIPRHPTDVRDLPPPSIVQMFVDFRTHTLKADARGEQAGEDTWQVADGYVLWYTRVSHP 334
QTIP D + I ++ + H + A G + Y+ W+ R SHP
Sbjct: 223 QTIPASTVD-SWVSYDDIHDRWMHYEDHIVPAGEVCVVPG----ACSSDYIDWFFRTSHP 59
Query: 335 QILP-----PIP-GDLPRP 347
+ P P+P G P+P
Sbjct: 58 FMTPGHAVDPLPHGHAPQP 2
>BQ576456
Length = 424
Score = 64.7 bits (156), Expect = 6e-11
Identities = 46/150 (30%), Positives = 66/150 (43%)
Frame = +1
Query: 4 GEMTVTLDDVACLTHLSIEGRMLAHGKKMPKHEGAALLMTYLGVAQHEAEKICNQEYGGY 63
GE+T+TLDD+ACL HL I G L + + E LLM L V++ EA + + Y
Sbjct: 31 GELTITLDDMACLLHLPITG-ALHRFEPLGVDEAVLLLMELLEVSREEARAKIVRAHRAY 207
Query: 64 ISYPRLRDFYTSYLGRANVLAGTEDPEEVEELERVRTYCVRCYLLYLVGCLLFGDRSNKR 123
+ LR+ Y S R VR YL +LV C LF ++S
Sbjct: 208 VRLSWLREVYQSRC-----------------QARCWIVAVRAYLFHLVDCTLFANKSATH 336
Query: 124 IELIYLTTMADGYAGMRNYSWGAMTLAYLY 153
+ +++L D Y WG L ++Y
Sbjct: 337 VHVVHLKGF*D-LC*SGGYGWGVAALVHMY 423
>TC232778 similar to UP|Q9LNG5 (Q9LNG5) F21D18.16, partial (5%)
Length = 746
Score = 53.5 bits (127), Expect = 1e-07
Identities = 46/153 (30%), Positives = 68/153 (44%)
Frame = -1
Query: 4 GEMTVTLDDVACLTHLSIEGRMLAHGKKMPKHEGAALLMTYLGVAQHEAEKICNQEYGGY 63
GE T+TL DV L L +G L + + A L LGV E E G
Sbjct: 422 GEATITLQDVLILLGLRTDGAPLIGSTNL---DWADLCEELLGVRPQEGEI-----EGSV 267
Query: 64 ISYPRLRDFYTSYLGRANVLAGTEDPEEVEELERVRTYCVRCYLLYLVGCLLFGDRSNKR 123
+ L Y N+ D VE+L+R +R ++L +G ++F ++S+ R
Sbjct: 266 VKLSWL----AHYFSHINI-----DEGNVEQLQRF----IRAWILRFIGGVIFVNKSSSR 126
Query: 124 IELIYLTTMADGYAGMRNYSWGAMTLAYLYGEL 156
+ L YL + D + Y+WG LAYLY E+
Sbjct: 125 VSLRYLQFLRD-FE*CSTYAWGPAMLAYLYREM 30
>TC211858
Length = 524
Score = 52.4 bits (124), Expect = 3e-07
Identities = 27/77 (35%), Positives = 38/77 (49%), Gaps = 3/77 (3%)
Frame = -3
Query: 170 SVTLLTAWFLAHFPGFFSVDLNTDYLENYSVAARWKLQKGHGEGIT---YRSLLDRIQLD 226
++ L W HFP ++ Y E A+RW K H +GIT YR+ D + +
Sbjct: 252 NLIFL*CWIYEHFPSVHQCVIDDTY*ETSPRASRWLTSKAHMKGITGAPYRARCDALTVI 73
Query: 227 DVCWRSYEEHREIQDFE 243
DV W Y EHR ++ FE
Sbjct: 72 DVSWLPYTEHRGVRAFE 22
Score = 35.4 bits (80), Expect(2) = 3e-04
Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 1/57 (1%)
Frame = -1
Query: 124 IELIYLTTMAD-GYAGMRNYSWGAMTLAYLYGELADACRLGHRALGGSVTLLTAWFL 179
+ ++YL D G +G Y+WG L ++Y +L +A R R + G +TLL F+
Sbjct: 470 VHVVYLDAFRDLGQSG--GYAWGVAALVHMYNQLDEASRTTTRQIVGYLTLLQVNFV 306
Score = 26.2 bits (56), Expect(2) = 3e-04
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = -2
Query: 106 YLLYLVGCLLFGDRS 120
YLL+LVGC LF ++S
Sbjct: 523 YLLHLVGCTLFANKS 479
>CO979307
Length = 553
Score = 47.0 bits (110), Expect = 1e-05
Identities = 41/172 (23%), Positives = 77/172 (43%), Gaps = 2/172 (1%)
Frame = -3
Query: 109 YLVGCLLFGDRSNKRIELIYLTTMADGYAGMRNYSWGAMTLAYLYGELADACRLGHRALG 168
+L+ ++F D+S + + YL +++ G L YLY L+ ++ G
Sbjct: 551 HLISSMIFADKSLAHVHVAYL*YLSN-LTACHK*V*GVAALVYLYDHLSYVSLDDNKQCG 375
Query: 169 GSVTLLTAWFLAHFP--GFFSVDLNTDYLENYSVAARWKLQKGHGEGITYRSLLDRIQLD 226
+TLL A H P G+ + + DY + + RW G + + ++ I ++
Sbjct: 374 DYMTLLMA*VFEHLPSVGYSNPE---DYSDGDPLNTRWNPLGGTRHALLVKERMNNIHMN 204
Query: 227 DVCWRSYEEHREIQDFEEVFWYSRWIMCGVRRVYRHLPERVLRQYGYVQTIP 278
YE ++ ++ F E + C + + +L +RV RQ+ +VQTIP
Sbjct: 203 VFI*CPYE*NKLVRPFVEQTL*FGYFKCRI-DMRSYLLKRVRRQFCHVQTIP 51
>BG881451 weakly similar to PIR|D96521|D96 protein F21D18.16 [imported] -
Arabidopsis thaliana, partial (1%)
Length = 421
Score = 37.7 bits (86), Expect = 0.008
Identities = 23/70 (32%), Positives = 37/70 (52%), Gaps = 5/70 (7%)
Frame = +3
Query: 214 ITYRSLLDRIQLDDVCWRSYE--EHREIQDFEE---VFWYSRWIMCGVRRVYRHLPERVL 268
+ YR LD ++ DV W Y + I ++ + + S+ ++ + RHLP R L
Sbjct: 6 VFYRKSLDSLKPCDVEWLPYRNMDSMVIPEYIKSTLILGRSKTMLICFDKAERHLPNRCL 185
Query: 269 RQYGYVQTIP 278
RQYG +Q+IP
Sbjct: 186 RQYGMLQSIP 215
>BF071225 weakly similar to PIR|D96521|D96 protein F21D18.16 [imported] -
Arabidopsis thaliana, partial (4%)
Length = 359
Score = 34.3 bits (77), Expect = 0.089
Identities = 24/84 (28%), Positives = 38/84 (44%), Gaps = 5/84 (5%)
Frame = +2
Query: 209 GHGEGITYRSLLDRIQLDDVCWRSYEEH-----REIQDFEEVFWYSRWIMCGVRRVYRHL 263
G+ + +R LD ++ + W Y +I V W++ + V H
Sbjct: 29 GNDDLRVFRRKLDLMKRHEFVWEPYTPTVMAALPQICVVGSVVWFAVVPLICFHVVEWHQ 208
Query: 264 PERVLRQYGYVQTIPRHPTDVRDL 287
P+RVLRQYG Q IP P+ ++L
Sbjct: 209 PDRVLRQYGLQQPIPGCPSQPQNL 280
>TC234144
Length = 468
Score = 33.9 bits (76), Expect = 0.12
Identities = 14/39 (35%), Positives = 21/39 (52%)
Frame = +3
Query: 217 RSLLDRIQLDDVCWRSYEEHREIQDFEEVFWYSRWIMCG 255
R LD + D+ W Y HRE + F ++ +S +I CG
Sbjct: 6 RPALDML*TKDILWMPYTVHREHRPFHDISLFSGYIRCG 122
>TC220808 weakly similar to UP|Q9SK32 (Q9SK32) Expressed protein, partial
(9%)
Length = 869
Score = 32.0 bits (71), Expect = 0.44
Identities = 30/97 (30%), Positives = 43/97 (43%), Gaps = 9/97 (9%)
Frame = +1
Query: 249 SRWIMCGVRRVYRHLPERVLRQYGYVQTIPRHPTDVRDLPPPS-IVQMFVDFRTHTLKAD 307
SR ++ + RHLP+R LRQ+ QTIP+ DV S IV VD K D
Sbjct: 22 SRTMLLCFDKAERHLPDRCLRQFAMHQTIPK---DVERWERKSRIVDHGVDLMG---KMD 183
Query: 308 ARGEQAGEDTWQVADG--------YVLWYTRVSHPQI 336
++ E V +G Y+ WY +++ I
Sbjct: 184 LALKEWSERWVHVVEGGDIVDEGEYMQWYQKITRKYI 294
>TC232239
Length = 747
Score = 28.5 bits (62), Expect = 4.9
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +2
Query: 203 RWKLQKGHGEGITYRSLLDRIQLDDVCWRSYEEH 236
+W Q G+GE +TY LD + + WR +E H
Sbjct: 458 KWGQQVGYGENLTYLLTLDYLN-SIMEWRRFEPH 556
>TC224671 similar to UP|Q8S8Z0 (Q8S8Z0) Protein phosphatase 2C, partial (87%)
Length = 1803
Score = 27.7 bits (60), Expect = 8.3
Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = +3
Query: 234 EEHREIQDFEE-VFWYSRWIMCGVRRVYRHLPERVLRQY 271
+E R I+D V W W + GV V R +R+L+QY
Sbjct: 999 DERRRIEDAGGFVMWAGTWRVGGVLAVSRAFGDRLLKQY 1115
>BE659518
Length = 784
Score = 27.7 bits (60), Expect = 8.3
Identities = 28/131 (21%), Positives = 46/131 (34%), Gaps = 19/131 (14%)
Frame = -3
Query: 121 NKRIELIYLTTMADGYA-----GMRNYSWGAMTLAYLYGELADACRLGHRAL-------- 167
NK ++ G+A +R YSWG+ L + G ++ CR+
Sbjct: 761 NKLCS*AHIXXXXXGHA*SINESLRGYSWGS*XLRWKIGSSSNRCRVA*SGFPCRRIAKD 582
Query: 168 -GGSVTLL-----TAWFLAHFPGFFSVDLNTDYLENYSVAARWKLQKGHGEGITYRSLLD 221
G V WF ++F G + + L S+ WK +R D
Sbjct: 581 *GSPVCFKRPFF*KPWFSSNFSGLYGIHL*GSV*VTCSI---WKQNTRGDSSDFFR*RKD 411
Query: 222 RIQLDDVCWRS 232
+ D+C +S
Sbjct: 410 EVSCYDMCQQS 378
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.323 0.140 0.447
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,134,395
Number of Sequences: 63676
Number of extensions: 271831
Number of successful extensions: 1250
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 1239
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1244
length of query: 357
length of database: 12,639,632
effective HSP length: 98
effective length of query: 259
effective length of database: 6,399,384
effective search space: 1657440456
effective search space used: 1657440456
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 59 (27.3 bits)
Medicago: description of AC125478.7