BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124972.11 - phase: 0 /pseudo
(127 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC230436 weakly similar to GB|AAM10372.1|20147317|AY093748 AT5g6... 242 3e-65
TC233758 weakly similar to GB|AAM10372.1|20147317|AY093748 AT5g6... 115 2e-39
BI970307 101 4e-25
AW830180 56 4e-09
TC211076 homologue to UP|Q948P2 (Q948P2) Replication factor C 40... 34 0.016
TC207272 similar to UP|Q9SNJ4 (Q9SNJ4) ESTs AU065232(E60855), pa... 34 0.021
TC230904 similar to UP|Q6NMK6 (Q6NMK6) At4g25280, partial (35%) 33 0.027
TC217399 similar to UP|KADC_ARATH (Q9ZUU1) Probable adenylate ki... 32 0.060
TC216249 weakly similar to UP|SAP1_YEAST (P39955) SAP1 protein, ... 32 0.078
TC204915 weakly similar to GB|AAH46286.1|28279482|BC046286 spast... 32 0.10
TC228922 similar to UP|KADD_ARATH (Q9FIJ7) Probable adenylate ki... 32 0.10
TC204913 weakly similar to UP|SAP1_YEAST (P39955) SAP1 protein, ... 32 0.10
BE660082 31 0.13
TC216546 weakly similar to UP|SAP1_YEAST (P39955) SAP1 protein, ... 31 0.13
TC230029 homologue to GB|AAQ56811.1|34098857|BT010368 At1g21690 ... 31 0.13
TC230028 homologue to GB|AAQ56811.1|34098857|BT010368 At1g21690 ... 31 0.13
BF070568 homologue to GP|10798790|dbj replication factor C 36kDa... 31 0.17
TC215430 similar to UP|UMPK_ARATH (O04905) Uridylate kinase (UK... 31 0.17
TC225683 homologue to UP|Q9SZD4 (Q9SZD4) 26S proteasome subunit ... 30 0.23
TC225681 homologue to UP|Q9SZD4 (Q9SZD4) 26S proteasome subunit ... 30 0.23
>TC230436 weakly similar to GB|AAM10372.1|20147317|AY093748
AT5g60340/k9b18_30 {Arabidopsis thaliana;} , partial
(89%)
Length = 842
Score = 242 bits (618), Expect = 3e-65
Identities = 112/127 (88%), Positives = 122/127 (95%)
Frame = +2
Query: 1 MVKDSIKRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDS 60
MV+++ KR+KPNILVTGTPGTGKTT TALAEATQL HIN+G+LVKEKNLHDGWDDELD
Sbjct: 89 MVQENGKRKKPNILVTGTPGTGKTTVCTALAEATQLCHINVGELVKEKNLHDGWDDELDC 268
Query: 61 YILNEDLVCDELEDVMDEGGNIVDYHGCDFFPERWFDCVVVLQTDNTILYDRLSRRGYKE 120
Y+LNEDLVCDELEDVM+EGGNIVDYHGCDFFPERWFDCVVVLQTDNTILYDRLSRRGYK+
Sbjct: 269 YLLNEDLVCDELEDVMEEGGNIVDYHGCDFFPERWFDCVVVLQTDNTILYDRLSRRGYKD 448
Query: 121 SKLSNNV 127
SKLSNN+
Sbjct: 449 SKLSNNI 469
>TC233758 weakly similar to GB|AAM10372.1|20147317|AY093748
AT5g60340/k9b18_30 {Arabidopsis thaliana;} , partial
(34%)
Length = 475
Score = 115 bits (288), Expect(2) = 2e-39
Identities = 52/62 (83%), Positives = 55/62 (87%)
Frame = +1
Query: 52 DGWDDELDSYILNEDLVCDELEDVMDEGGNIVDYHGCDFFPERWFDCVVVLQTDNTILYD 111
DGWD L Y+LNEDLVCDELEDVM+ GGNIVDYHGC FFPERW +CVVVLQTDNTILYD
Sbjct: 289 DGWDLGLHCYLLNEDLVCDELEDVMEAGGNIVDYHGCVFFPERWSNCVVVLQTDNTILYD 468
Query: 112 RL 113
RL
Sbjct: 469 RL 474
Score = 62.8 bits (151), Expect(2) = 2e-39
Identities = 28/34 (82%), Positives = 30/34 (87%)
Frame = +3
Query: 21 TGKTTTSTALAEATQLNHINIGDLVKEKNLHDGW 54
TGKTT TALAEATQL HIN+G+LVK KNLHDGW
Sbjct: 192 TGKTTVCTALAEATQLRHINVGELVKGKNLHDGW 293
>BI970307
Length = 660
Score = 101 bits (251), Expect(2) = 4e-25
Identities = 45/49 (91%), Positives = 47/49 (95%)
Frame = +2
Query: 68 VCDELEDVMDEGGNIVDYHGCDFFPERWFDCVVVLQTDNTILYDRLSRR 116
VCDELEDVM+ GGNIVDYHGC FFPERWF+CVVVLQTDNTILYDRLSRR
Sbjct: 167 VCDELEDVMEAGGNIVDYHGCVFFPERWFNCVVVLQTDNTILYDRLSRR 313
Score = 28.9 bits (63), Expect(2) = 4e-25
Identities = 11/18 (61%), Positives = 15/18 (83%)
Frame = +1
Query: 110 YDRLSRRGYKESKLSNNV 127
+ R+ RGYK+SKLSNN+
Sbjct: 391 FPRICDRGYKDSKLSNNI 444
>AW830180
Length = 358
Score = 56.2 bits (134), Expect = 4e-09
Identities = 26/32 (81%), Positives = 28/32 (87%)
Frame = +3
Query: 21 TGKTTTSTALAEATQLNHINIGDLVKEKNLHD 52
TGKTT TALAEATQL HIN+G+LVK KNLHD
Sbjct: 261 TGKTTVCTALAEATQLRHINVGELVKGKNLHD 356
>TC211076 homologue to UP|Q948P2 (Q948P2) Replication factor C 40kDa subunit,
partial (40%)
Length = 504
Score = 34.3 bits (77), Expect = 0.016
Identities = 19/42 (45%), Positives = 25/42 (59%)
Frame = +3
Query: 11 PNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHD 52
PN++++G PGTGKTT+ ALA L N + V E N D
Sbjct: 210 PNLILSGPPGTGKTTSILALAHEL-LGGPNCKEAVLELNASD 332
>TC207272 similar to UP|Q9SNJ4 (Q9SNJ4) ESTs AU065232(E60855), partial (72%)
Length = 1446
Score = 33.9 bits (76), Expect = 0.021
Identities = 21/71 (29%), Positives = 33/71 (45%), Gaps = 1/71 (1%)
Frame = +2
Query: 3 KDSIKRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVK-EKNLHDGWDDELDSY 61
++ + +R G PG GK T ++ L+ + HI GDLV+ E D +L
Sbjct: 176 REDLSKRCVQWAFLGCPGVGKGTYASRLSNLLGVPHIATGDLVRDELASSDPLSSQLSEI 355
Query: 62 ILNEDLVCDEL 72
+ LV DE+
Sbjct: 356 VKQGQLVSDEI 388
>TC230904 similar to UP|Q6NMK6 (Q6NMK6) At4g25280, partial (35%)
Length = 421
Score = 33.5 bits (75), Expect = 0.027
Identities = 20/62 (32%), Positives = 30/62 (48%)
Frame = +1
Query: 3 KDSIKRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYI 62
KD I + V G PG+GK T + E H++ GDL++ + + D E S I
Sbjct: 136 KDGICPKPLITFVLGGPGSGKGTQCGKIVETFGFKHLSAGDLLRREMVS---DSEYGSMI 306
Query: 63 LN 64
+N
Sbjct: 307 MN 312
>TC217399 similar to UP|KADC_ARATH (Q9ZUU1) Probable adenylate kinase 1,
chloroplast precursor (ATP-AMP transphosphorylase) ,
partial (83%)
Length = 1188
Score = 32.3 bits (72), Expect = 0.060
Identities = 19/57 (33%), Positives = 28/57 (48%), Gaps = 1/57 (1%)
Frame = +3
Query: 17 GTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDG-WDDELDSYILNEDLVCDEL 72
G PG GK T ++ L + HI GDLV+ + +G +L + LV DE+
Sbjct: 144 GCPGVGKGTYASRLCNLLGVPHIATGDLVRHELASNGPLSSQLSEIVNQGKLVSDEI 314
>TC216249 weakly similar to UP|SAP1_YEAST (P39955) SAP1 protein, partial (14%)
Length = 2222
Score = 32.0 bits (71), Expect = 0.078
Identities = 19/60 (31%), Positives = 28/60 (46%)
Frame = +2
Query: 3 KDSIKRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYI 62
K + + IL+ G PGTGKT + A+A N INI ++ W E + Y+
Sbjct: 1049 KGQLTKPCKGILLFGPPGTGKTMLAKAIATEAGANFINI----SMSSITSKWFGEGEKYV 1216
>TC204915 weakly similar to GB|AAH46286.1|28279482|BC046286 spastic
paraplegia 4 homolog {Mus musculus;} , partial (21%)
Length = 1470
Score = 31.6 bits (70), Expect = 0.10
Identities = 19/60 (31%), Positives = 28/60 (46%)
Frame = +1
Query: 3 KDSIKRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYI 62
K + + IL+ G PGTGKT + A+A N INI ++ W E + Y+
Sbjct: 364 KGQLAKPCKGILLFGPPGTGKTMLAKAVATEAGANFINI----SMSSITSKWFGEGEKYV 531
>TC228922 similar to UP|KADD_ARATH (Q9FIJ7) Probable adenylate kinase 2,
chloroplast precursor (ATP-AMP transphosphorylase) ,
partial (75%)
Length = 1133
Score = 31.6 bits (70), Expect = 0.10
Identities = 21/74 (28%), Positives = 36/74 (48%), Gaps = 2/74 (2%)
Frame = +3
Query: 5 SIKRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDD--ELDSYI 62
S+ N++++G P +GK T + L HI GDL++ + + G D+ Y+
Sbjct: 213 SVTNEALNVMISGAPASGKGTQCHLITNKYGLVHIAAGDLLRAE-IATGSDNGKRAKQYM 389
Query: 63 LNEDLVCDELEDVM 76
LV DE+ +M
Sbjct: 390 EKGQLVPDEIVVMM 431
>TC204913 weakly similar to UP|SAP1_YEAST (P39955) SAP1 protein, partial
(14%)
Length = 1080
Score = 31.6 bits (70), Expect = 0.10
Identities = 19/60 (31%), Positives = 28/60 (46%)
Frame = +3
Query: 3 KDSIKRRKPNILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYI 62
K + + IL+ G PGTGKT + A+A N INI ++ W E + Y+
Sbjct: 393 KGQLAKPCKGILLFGPPGTGKTMLAKAVATEAGANFINI----SMSSITSKWFGEGEKYV 560
>BE660082
Length = 846
Score = 31.2 bits (69), Expect = 0.13
Identities = 16/54 (29%), Positives = 26/54 (47%), Gaps = 3/54 (5%)
Frame = -2
Query: 40 NIGDLVKEKNLHD---GWDDELDSYILNEDLVCDELEDVMDEGGNIVDYHGCDF 90
++GD+ + + LHD GW L ++ + +L C E + EG N C F
Sbjct: 506 SLGDISRARWLHDFF*GWSTLLPTFCSSNELFCFETTQQLVEGPNQAPLPACPF 345
>TC216546 weakly similar to UP|SAP1_YEAST (P39955) SAP1 protein, partial
(14%)
Length = 1321
Score = 31.2 bits (69), Expect = 0.13
Identities = 18/50 (36%), Positives = 25/50 (50%)
Frame = +3
Query: 13 ILVTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDELDSYI 62
IL+ G PGTGKT + A+A N INI ++ W E + Y+
Sbjct: 150 ILLFGPPGTGKTMLAKAVATEAGANFINI----SMSSITSKWFGEGEKYV 287
>TC230029 homologue to GB|AAQ56811.1|34098857|BT010368 At1g21690 {Arabidopsis
thaliana;} , partial (47%)
Length = 687
Score = 31.2 bits (69), Expect = 0.13
Identities = 13/21 (61%), Positives = 17/21 (80%)
Frame = +1
Query: 11 PNILVTGTPGTGKTTTSTALA 31
P++L G PGTGKTTT+ A+A
Sbjct: 340 PHMLFYGPPGTGKTTTALAIA 402
>TC230028 homologue to GB|AAQ56811.1|34098857|BT010368 At1g21690 {Arabidopsis
thaliana;} , partial (24%)
Length = 435
Score = 31.2 bits (69), Expect = 0.13
Identities = 13/21 (61%), Positives = 17/21 (80%)
Frame = +1
Query: 11 PNILVTGTPGTGKTTTSTALA 31
P++L G PGTGKTTT+ A+A
Sbjct: 322 PHMLFYGPPGTGKTTTALAIA 384
>BF070568 homologue to GP|10798790|dbj replication factor C 36kDa subunit
{Oryza sativa (japonica cultivar-group)}, partial (15%)
Length = 348
Score = 30.8 bits (68), Expect = 0.17
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = +3
Query: 7 KRRKPNILVTGTPGTGKTTTSTALA 31
+ R P++L+ G PGTGKT+T A+A
Sbjct: 273 ENRLPHLLLYGPPGTGKTSTILAVA 347
>TC215430 similar to UP|UMPK_ARATH (O04905) Uridylate kinase (UK) (Uridine
monophosphate kinase) (UMP kinase) (UMP/CMP kinase) ,
partial (93%)
Length = 1156
Score = 30.8 bits (68), Expect = 0.17
Identities = 25/96 (26%), Positives = 44/96 (45%), Gaps = 8/96 (8%)
Frame = +3
Query: 5 SIKRRKPNIL-VTGTPGTGKTTTSTALAEATQLNHINIGDLVKEKNLHDGWDDEL----- 58
S+ + P ++ V G PG+GK T + E H++ GDL++ + + G ++
Sbjct: 228 SLLEKNPTVVFVLGGPGSGKGTQCANIVENFGYTHLSAGDLLRAE-IKSGSENGTMIQNM 404
Query: 59 --DSYILNEDLVCDELEDVMDEGGNIVDYHGCDFFP 92
+ I+ ++ L+ M E GN D D FP
Sbjct: 405 IKEGKIVPSEVTIKLLQKAMQESGN--DKFLIDGFP 506
>TC225683 homologue to UP|Q9SZD4 (Q9SZD4) 26S proteasome subunit 4-like
protein (26S proteasome subunit AtRPT2a), partial (97%)
Length = 1513
Score = 30.4 bits (67), Expect = 0.23
Identities = 15/42 (35%), Positives = 25/42 (58%), Gaps = 1/42 (2%)
Frame = +2
Query: 13 ILVTGTPGTGKTTTSTALAEATQLNHIN-IGDLVKEKNLHDG 53
+++ G PGTGKT + A+A +T + +G + +K L DG
Sbjct: 683 VILYGEPGTGKTLLAKAVANSTSATFLRVVGSELIQKYLGDG 808
>TC225681 homologue to UP|Q9SZD4 (Q9SZD4) 26S proteasome subunit 4-like
protein (26S proteasome subunit AtRPT2a), partial (93%)
Length = 1433
Score = 30.4 bits (67), Expect = 0.23
Identities = 15/42 (35%), Positives = 25/42 (58%), Gaps = 1/42 (2%)
Frame = +1
Query: 13 ILVTGTPGTGKTTTSTALAEATQLNHIN-IGDLVKEKNLHDG 53
+++ G PGTGKT + A+A +T + +G + +K L DG
Sbjct: 580 VILYGEPGTGKTLLAKAVANSTSATFLRVVGSELIQKYLGDG 705
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.316 0.137 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,356,401
Number of Sequences: 63676
Number of extensions: 68738
Number of successful extensions: 402
Number of sequences better than 10.0: 95
Number of HSP's better than 10.0 without gapping: 401
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 402
length of query: 127
length of database: 12,639,632
effective HSP length: 86
effective length of query: 41
effective length of database: 7,163,496
effective search space: 293703336
effective search space used: 293703336
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)
Medicago: description of AC124972.11