BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124970.11 + phase: 0
(211 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ133845 124 2e-29
TC232398 62 1e-10
CO985236 36 0.015
TC219310 34 0.043
TC225759 similar to UP|Q9SMK5 (Q9SMK5) Plasma membrane intrinsic... 33 0.073
TC231486 similar to UP|Q9LNR9 (Q9LNR9) F1L3.5, partial (28%) 33 0.12
CF922292 32 0.28
TC210783 weakly similar to UP|O14760 (O14760) Small intestinal m... 31 0.36
BQ628408 similar to GP|8347238|gb|A heat stress transcription fa... 31 0.47
TC210538 similar to UP|H1_MAIZE (P23444) Histone H1, partial (29%) 31 0.47
AI900250 31 0.47
TC218960 weakly similar to UP|Q6K674 (Q6K674) Translation initia... 31 0.47
BG649872 30 0.61
CA936008 30 0.61
BE661138 weakly similar to GP|21779917|gb| leucine-rich-like pro... 30 0.80
TC225703 homologue to UP|Q9ZRD8 (Q9ZRD8) GMFP5 (Fragment), parti... 30 0.80
BU763414 similar to GP|20521491|dbj B1147B04.8 {Oryza sativa (ja... 30 0.80
TC216196 weakly similar to UP|BRAT_DROME (Q8MQJ9) Brain tumor pr... 29 1.4
TC218867 homologue to UP|Q43444 (Q43444) Phosphoinositide-specif... 29 1.4
BM892171 similar to GP|16930681|gb| At2g05170/F5G3.7 {Arabidopsi... 29 1.8
>BQ133845
Length = 389
Score = 124 bits (312), Expect = 2e-29
Identities = 61/120 (50%), Positives = 84/120 (69%)
Frame = +2
Query: 76 KLPYPHLAKNKKKEEGQFKKFMQLFSQLQVNIPFGEALDQMPVYAKFMKEMLTSRRKPKD 135
++PYP + K KE+ KF+ +F +L++ +PF EAL QMP+YA F+K+MLT +
Sbjct: 32 EVPYPLVPS*KDKEQ-HLAKFLDIFKKLEITLPFEEALQQMPLYANFLKDMLTKKNWYIH 208
Query: 136 DENIALSENCSAILQRKLPPKLKDPGAFTIPCSIGPVDIGRALCDLGASINLMPLSMMKK 195
+ I + NCSA++QR LPP DPG T+PCSIG V +G+AL DLGASINLMPLSM ++
Sbjct: 209 SDKIVVEGNCSAVIQRILPP*HTDPGFVTMPCSIGEVAVGKALIDLGASINLMPLSMCRQ 388
>TC232398
Length = 1054
Score = 62.4 bits (150), Expect = 1e-10
Identities = 29/59 (49%), Positives = 41/59 (69%)
Frame = +2
Query: 103 LQVNIPFGEALDQMPVYAKFMKEMLTSRRKPKDDENIALSENCSAILQRKLPPKLKDPG 161
L++ IPFGE + QMP+Y KF+K++L + K + E I + E C A++Q KLPPK KD G
Sbjct: 2 LEITIPFGERIQQMPLYKKFLKDILIKKGKYINSETIVVGEYCRALIQ-KLPPKFKDLG 175
>CO985236
Length = 466
Score = 35.8 bits (81), Expect = 0.015
Identities = 20/58 (34%), Positives = 37/58 (63%), Gaps = 8/58 (13%)
Frame = -1
Query: 2 EVDKDVEVKEVVEKETEHGEVEKESDQGVVE--------NERKKKKGGEKSEKLIDED 51
++D+D E+KE +E E E+G ++ES++ VV+ E+++ K EK E +++ED
Sbjct: 193 KMDEDTEIKETMEGEPENG--KEESEKPVVDAMEVEGGIKEKEESKENEKVEVVMEED 26
Score = 28.5 bits (62), Expect = 2.3
Identities = 25/85 (29%), Positives = 39/85 (45%), Gaps = 3/85 (3%)
Frame = -1
Query: 9 VKEVVEKETEHGEVEKESDQ---GVVENERKKKKGGEKSEKLIDEDSILRKSKSQILKDG 65
V+E E + E G E D+ GV E + K+ G K ++ED + K K G
Sbjct: 430 VEEATEDKKEDGVEEVNEDRKEDGVGEVKEDKEVDGVSCVKEVEEDGEVDGVKKVEDKKG 251
Query: 66 DKPQVIPSYVKLPYPHLAKNKKKEE 90
D+ + I K H+++N K +E
Sbjct: 250 DELKEIEEDKK--DDHISENDKMDE 182
>TC219310
Length = 888
Score = 34.3 bits (77), Expect = 0.043
Identities = 20/60 (33%), Positives = 34/60 (56%), Gaps = 7/60 (11%)
Frame = +1
Query: 5 KDVEVKEVVEKETE----HGEVEKESDQGVVENERKKKKGGEKSEK---LIDEDSILRKS 57
++VEV E++EK+ E +G Q + N+RK+K+ + EK L +++ IL KS
Sbjct: 472 EEVEVTEIIEKDEENVKGNGSTNDLKTQAITVNKRKRKRSTQVKEKIDPLKEDNGILAKS 651
>TC225759 similar to UP|Q9SMK5 (Q9SMK5) Plasma membrane intrinsic
polypeptide, partial (74%)
Length = 1051
Score = 33.5 bits (75), Expect = 0.073
Identities = 26/70 (37%), Positives = 36/70 (51%), Gaps = 5/70 (7%)
Frame = +1
Query: 4 DKDVEVKEVVEKETEH--GEVEKESDQGVVENERKKKKGGEKSEKLI---DEDSILRKSK 58
+KDVE VE +TE V KE + VVE E+KK+K E+ ++I DE + K
Sbjct: 514 EKDVEAPPGVETKTEEETSSVVKEREI-VVEEEKKKEKKEEEKPQVIETSDEKKVEEKPA 690
Query: 59 SQILKDGDKP 68
K +KP
Sbjct: 691 ETSAKGEEKP 720
>TC231486 similar to UP|Q9LNR9 (Q9LNR9) F1L3.5, partial (28%)
Length = 1024
Score = 32.7 bits (73), Expect = 0.12
Identities = 35/135 (25%), Positives = 56/135 (40%), Gaps = 1/135 (0%)
Frame = +2
Query: 4 DKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSI-LRKSKSQIL 62
DKD E +E EKE E ++EK V + K+ S+K I + SI K +
Sbjct: 347 DKDEEKEEEREKEEEEEDIEKTLS---VILGKFKEICANNSKKAIGKKSISFLLKKMFVC 517
Query: 63 KDGDKPQVIPSYVKLPYPHLAKNKKKEEGQFKKFMQLFSQLQVNIPFGEALDQMPVYAKF 122
+ G P P L + +E + +K ++ ++N Q A
Sbjct: 518 RSGFAPA----------PSLRDTLQLQESRMEKLLRTILHKKIN-------SQHSSRALS 646
Query: 123 MKEMLTSRRKPKDDE 137
+K+ L R+ PK+DE
Sbjct: 647 LKKRLEDRKMPKEDE 691
>CF922292
Length = 722
Score = 31.6 bits (70), Expect = 0.28
Identities = 18/60 (30%), Positives = 33/60 (55%), Gaps = 4/60 (6%)
Frame = -2
Query: 4 DKDVEVKEVVEKETEHGEVEKESDQGVVENE----RKKKKGGEKSEKLIDEDSILRKSKS 59
DK+ +VKE E++++ EK S+ EN+ +K+ ++S++ D D +KS S
Sbjct: 343 DKNSDVKEKQEEKSDEKSQEKPSEDTKTENQDTSVSEKRSDSDESQQKSDSDESQQKSDS 164
>TC210783 weakly similar to UP|O14760 (O14760) Small intestinal mucin MUC3
(Fragment), partial (5%)
Length = 735
Score = 31.2 bits (69), Expect = 0.36
Identities = 24/84 (28%), Positives = 39/84 (45%), Gaps = 1/84 (1%)
Frame = +3
Query: 9 VKEVVEKETEHGE-VEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQILKDGDK 67
VKE+ +K+ E VE++ GVVE+ ++ KK E D+ ++ + K +DG +
Sbjct: 357 VKEIEDKKAVGVEKVEEDKKNGVVEDAKEDKKEDGVKEAKEDKKEVVEEVKEDNKEDGVE 536
Query: 68 PQVIPSYVKLPYPHLAKNKKKEEG 91
AK KKE+G
Sbjct: 537 E--------------AKENKKEDG 566
>BQ628408 similar to GP|8347238|gb|A heat stress transcription factor A3
{Lycopersicon peruvianum}, partial (7%)
Length = 446
Score = 30.8 bits (68), Expect = 0.47
Identities = 19/89 (21%), Positives = 42/89 (46%)
Frame = +1
Query: 8 EVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQILKDGDK 67
EV ++ +++ E +Q + E+++K+ KLI + L + + + K+ D
Sbjct: 133 EVVDLKQEQRRMAHRAGEVNQRLQSTEQRQKQMVSFLVKLIQNPAFLARLRHEEQKEIDS 312
Query: 68 PQVIPSYVKLPYPHLAKNKKKEEGQFKKF 96
P+V+ +VK + +EGQ ++
Sbjct: 313 PRVVRKFVKQHQHETGTAETLQEGQIVRY 399
>TC210538 similar to UP|H1_MAIZE (P23444) Histone H1, partial (29%)
Length = 643
Score = 30.8 bits (68), Expect = 0.47
Identities = 25/100 (25%), Positives = 41/100 (41%), Gaps = 14/100 (14%)
Frame = +2
Query: 2 EVDKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKL-------------- 47
+V ++ +KET+ E E Q V + ++KKKK GEK ++L
Sbjct: 182 KVKNSYKLSSATDKETQKN-TETEPKQKVEKKKKKKKKVGEKIKRLSQVKTPETLKKKAP 358
Query: 48 IDEDSILRKSKSQILKDGDKPQVIPSYVKLPYPHLAKNKK 87
+D+ K KS +G + ++ P K KK
Sbjct: 359 TKKDAAATKKKSASAVEGSGGGKVKCLSQVKTPEALKKKK 478
>AI900250
Length = 411
Score = 30.8 bits (68), Expect = 0.47
Identities = 17/36 (47%), Positives = 23/36 (63%), Gaps = 1/36 (2%)
Frame = -2
Query: 7 VEVKEVVEKETEHGEVEKES-DQGVVENERKKKKGG 41
++ E EK+ E E +K+ DQG V N+ KKKKGG
Sbjct: 158 IDSTEGEEKQREEREEKKQQEDQGGV*NDMKKKKGG 51
>TC218960 weakly similar to UP|Q6K674 (Q6K674) Translation initiation factor
IF-3-like, partial (76%)
Length = 1461
Score = 30.8 bits (68), Expect = 0.47
Identities = 40/179 (22%), Positives = 69/179 (38%), Gaps = 18/179 (10%)
Frame = +2
Query: 2 EVDKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKL-------------- 47
++ +D E+ V+ V + D + E++KKK G++
Sbjct: 305 QMAQDSELDLVIVSPDADPPVVRMMDYNKYKYEQQKKKKGQQKRSAANRMDLKELKMGYN 484
Query: 48 IDED--SILRKSKSQILKDGDKPQVIPSYVKLPYPHLAKNKKKEEGQFKKFMQLFSQLQV 105
ID+ S+ K+ + LKDGDK +VI N K E +F+
Sbjct: 485 IDQHDYSVRLKAAQKFLKDGDKVKVI------------VNLKGRETEFRNI--------- 601
Query: 106 NIPFGEALDQMPVYAKFMKEMLTSRRKPKDDEN--IALSENCSAILQRKLPPKLKDPGA 162
A++ + + + E+ T K D N I + +A+ + + PPK KD A
Sbjct: 602 ------AIELIKRFQSDVGELATEETKSFRDRNMSIVMVPKKAALQKAQEPPKRKDMSA 760
>BG649872
Length = 326
Score = 30.4 bits (67), Expect = 0.61
Identities = 18/58 (31%), Positives = 32/58 (55%), Gaps = 8/58 (13%)
Frame = -1
Query: 2 EVDKDVEVKEVVEKETEHGEVEKESDQ--------GVVENERKKKKGGEKSEKLIDED 51
++D+D EVKE +E + E+ +V+ E + G+ E E +K EK + ++ED
Sbjct: 197 KMDEDTEVKETIEGKQENEKVQAEKPEVDAMEVEGGIQEEESDEK---EKKDVAMEED 33
>CA936008
Length = 421
Score = 30.4 bits (67), Expect = 0.61
Identities = 24/91 (26%), Positives = 46/91 (50%), Gaps = 8/91 (8%)
Frame = +1
Query: 7 VEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEK--------LIDEDSILRKSK 58
V+ E +K+ + + +K+ + + ++KKKKGG ++K L D +L++ K
Sbjct: 34 VKASEKKKKKKKKKKKKKKKKKKKKKKKKKKKKGGGGTKKHPKKKRFSLADC*KLLKQKK 213
Query: 59 SQILKDGDKPQVIPSYVKLPYPHLAKNKKKE 89
K+ ++I Y P+P LAK K++
Sbjct: 214 LGKTKNLPTLKIIAQYP--PFPLLAKKPKRK 300
>BE661138 weakly similar to GP|21779917|gb| leucine-rich-like protein
{Aegilops tauschii}, partial (7%)
Length = 567
Score = 30.0 bits (66), Expect = 0.80
Identities = 17/41 (41%), Positives = 23/41 (55%)
Frame = -1
Query: 6 DVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEK 46
+VE+ EV E E HGE+ E+ G VEN +G E E+
Sbjct: 300 EVELLEVGEVEDRHGELASEAVVGQVENPEGFFQGREIRER 178
>TC225703 homologue to UP|Q9ZRD8 (Q9ZRD8) GMFP5 (Fragment), partial (79%)
Length = 1098
Score = 30.0 bits (66), Expect = 0.80
Identities = 22/69 (31%), Positives = 38/69 (54%)
Frame = +3
Query: 2 EVDKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQI 61
++ ++VEV +K+ + + EKE D G + E++K GGEK EK + S+S
Sbjct: 621 KLKRNVEVVPPPKKDDD--KKEKEGDGGE-KKEKEKDGGGEKKEKDVVLVRRRTLSRSNK 791
Query: 62 LKDGDKPQV 70
L+ G P++
Sbjct: 792 LRYGGSPRI 818
>BU763414 similar to GP|20521491|dbj B1147B04.8 {Oryza sativa (japonica
cultivar-group)}, partial (7%)
Length = 421
Score = 30.0 bits (66), Expect = 0.80
Identities = 16/60 (26%), Positives = 34/60 (56%)
Frame = -1
Query: 2 EVDKDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLIDEDSILRKSKSQI 61
E +++ EV E+ + E EH E E + D+G +++ ++ + +E + + R SKS++
Sbjct: 382 EAEEEDEVIEITDDE-EHEEAEDDEDEGDDDDQYREMEEEHSAEYMRSQSHPRRASKSKL 206
>TC216196 weakly similar to UP|BRAT_DROME (Q8MQJ9) Brain tumor protein,
partial (3%)
Length = 1679
Score = 29.3 bits (64), Expect = 1.4
Identities = 20/81 (24%), Positives = 41/81 (49%), Gaps = 7/81 (8%)
Frame = +3
Query: 9 VKEVVEKETEHGEVEKESDQGVV---ENERKKKKGGEKSEKL----IDEDSILRKSKSQI 61
VK +E+E ++++E ++ + + + + K ++ E+L ++ED L ++ +
Sbjct: 63 VKVHLEEEERLAKIQQEEEERLAKIQQEDERLAKIQQEDERLAKAQLEEDEQLARAIQES 242
Query: 62 LKDGDKPQVIPSYVKLPYPHL 82
LK G PQ L +PHL
Sbjct: 243 LKIGSPPQYDNGSSILSFPHL 305
>TC218867 homologue to UP|Q43444 (Q43444) Phosphoinositide-specific
phospholipase C P25 , complete
Length = 2341
Score = 29.3 bits (64), Expect = 1.4
Identities = 22/81 (27%), Positives = 35/81 (43%), Gaps = 1/81 (1%)
Frame = +3
Query: 7 VEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSE-KLIDEDSILRKSKSQILKDG 65
+E KEV EKE E + + D+ E +GG S+ K I++D +L + +
Sbjct: 786 LEAKEVQEKEEESQQEKPADDEEAWGKEVPSLRGGTISDYKNIEDDDVLDDEEDIDEAEK 965
Query: 66 DKPQVIPSYVKLPYPHLAKNK 86
+ Y +L H K K
Sbjct: 966 SRQDAADEYRRLIAIHAGKPK 1028
>BM892171 similar to GP|16930681|gb| At2g05170/F5G3.7 {Arabidopsis thaliana},
partial (7%)
Length = 425
Score = 28.9 bits (63), Expect = 1.8
Identities = 19/71 (26%), Positives = 36/71 (49%), Gaps = 4/71 (5%)
Frame = +2
Query: 5 KDVEVKEVVEKETEHGEVEKESDQGVVENERKKKKGGEKSEKLID----EDSILRKSKSQ 60
+DV V+EV GEV +E R++++GGE+ L++ LR+ +
Sbjct: 101 RDVSVEEV---RVLRGEVRREVRGSRERRRRRRRRGGEEDRVLLEW*GQTCHRLRRRRRL 271
Query: 61 ILKDGDKPQVI 71
+L+ G + Q++
Sbjct: 272 LLRSGPQVQLL 304
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.313 0.134 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,349,198
Number of Sequences: 63676
Number of extensions: 88861
Number of successful extensions: 540
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 525
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 536
length of query: 211
length of database: 12,639,632
effective HSP length: 93
effective length of query: 118
effective length of database: 6,717,764
effective search space: 792696152
effective search space used: 792696152
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC124970.11