BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124967.6 - phase: 0 /pseudo
(492 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC233421 similar to UP|Q7XZU1 (Q7XZU1) SAC domain protein 4, par... 332 2e-91
TC219912 similar to UP|Q7XZU1 (Q7XZU1) SAC domain protein 4, par... 318 5e-87
TC215714 similar to UP|Q94A27 (Q94A27) At3g14201 (At3g14201/at3g... 112 3e-59
TC222375 similar to UP|Q7XZU1 (Q7XZU1) SAC domain protein 4, par... 214 5e-56
TC215710 weakly similar to UP|Q7XZU2 (Q7XZU2) SAC domain protein... 65 1e-19
TC208487 similar to UP|Q94A27 (Q94A27) At3g14201 (At3g14201/at3g... 79 5e-15
AW306240 79 6e-15
TC207010 similar to GB|AAP49840.1|31415731|AY227250 SAC domain p... 74 1e-13
TC220765 weakly similar to UP|Q7X911 (Q7X911) SAC1-like protein ... 58 8e-09
BE610467 48 9e-06
TC213986 similar to UP|O23215 (O23215) Transcription initiation ... 29 4.2
CD392158 29 4.2
TC228225 weakly similar to UP|Q9FFJ7 (Q9FFJ7) Similarity to C3HC... 28 7.2
AW734844 28 7.2
TC234537 similar to UP|Q76DY1 (Q76DY1) AG-motif binding protein-... 28 7.2
>TC233421 similar to UP|Q7XZU1 (Q7XZU1) SAC domain protein 4, partial (26%)
Length = 665
Score = 332 bits (851), Expect = 2e-91
Identities = 163/176 (92%), Positives = 171/176 (96%)
Frame = +1
Query: 3 RYLRRGVNEKGRVANDVETEQIVFEDVPEGLPIKISSVIQNRGSIPLFWSQETSRLNIKP 62
RYLRRGVNEKGRVANDVETEQIVFEDV EGLPI+ISS+IQNRGSIPLFWSQETS+LNIKP
Sbjct: 136 RYLRRGVNEKGRVANDVETEQIVFEDVAEGLPIQISSIIQNRGSIPLFWSQETSKLNIKP 315
Query: 63 DIILSKKDQSYQATKLHFENLVKRYGNPIIILNLIKTHEKKPREAILRQEFANAIDFINK 122
DIILSKKDQ+YQAT+LHFENLVKRYGNPIIILNLIKTHEKKPRE+ILRQEFANAIDFINK
Sbjct: 316 DIILSKKDQNYQATRLHFENLVKRYGNPIIILNLIKTHEKKPRESILRQEFANAIDFINK 495
Query: 123 DLSEENRLRFLHWDLHKHFQSKATNVLLLLGKVAAYALTLTGFFYCQVSSTLRPED 178
DLSEENRLRFLHWDLHKHFQSKATNVL LLGKVAAYALTLT FFYCQ + TLRPE+
Sbjct: 496 DLSEENRLRFLHWDLHKHFQSKATNVLQLLGKVAAYALTLTSFFYCQTTPTLRPEE 663
>TC219912 similar to UP|Q7XZU1 (Q7XZU1) SAC domain protein 4, partial (24%)
Length = 823
Score = 318 bits (814), Expect = 5e-87
Identities = 168/265 (63%), Positives = 200/265 (75%), Gaps = 8/265 (3%)
Frame = +3
Query: 37 ISSVIQNRGSIPLFWSQETSRLNIKPDIILSKKDQSYQATKLHFENLVKRYGNPIIILNL 96
ISSV+Q RGSIPLFWSQE SRLNIKPDIILS+KD +++AT+LHFENLVKRYGNPIIILNL
Sbjct: 45 ISSVVQIRGSIPLFWSQEASRLNIKPDIILSRKDSNFEATRLHFENLVKRYGNPIIILNL 224
Query: 97 IKTHEKKPREAILRQEFANAIDFINKDLSEENRLRFLHWDLHKHFQ-SKATNVLLLLGKV 155
IKT EKKPRE ILR EFANA+ +NK+L ENRLRFLHWDLH+H + SKATNVL LGKV
Sbjct: 225 IKTREKKPRETILRAEFANAVRSLNKNLKGENRLRFLHWDLHRHSRCSKATNVLGQLGKV 404
Query: 156 AAYALTLTGFFYCQVSSTLRPEDCLKWPSTD--DVDKGSFSPTVHVEYDNEDANDLERKP 213
AAYAL LTG FYC V+S +R + + T+ +V + + DN D K
Sbjct: 405 AAYALKLTGIFYCPVTSNVRLDGFSHYSYTENNNVTDHCITEQASINKDNVD------KE 566
Query: 214 SDEINV-----SNENHSAKPPRLQNGVLRTNCIDCLDRTNVAQYAYGLAAIGHQLHSLGI 268
++ IN N+++S KP LQ+GVLRTNCIDCLDRTNVAQYAYGLAA+G+QL +LG
Sbjct: 567 TEIINCYCSSDENKDYSVKPQMLQSGVLRTNCIDCLDRTNVAQYAYGLAALGYQLQALGF 746
Query: 269 IEHPKIDLDDPVANDLMQFYERMGD 293
E P IDLD+P+A +LM+ YE MGD
Sbjct: 747 TETPNIDLDNPLAKELMEIYESMGD 821
>TC215714 similar to UP|Q94A27 (Q94A27) At3g14201 (At3g14201/at3g14201) (SAC
domain protein 2), partial (8%)
Length = 1224
Score = 112 bits (281), Expect(3) = 3e-59
Identities = 53/59 (89%), Positives = 54/59 (90%)
Frame = +3
Query: 323 QEFFRTLQRYYSNAWMDAEKQDAINVFLGHFQPQQDKPALWELGSDQHYDTGRIGDDDA 381
QEFFRTLQRYYSNA+MDA KQDAINVFLGHFQPQQ KPALWELGSDQ YD GR GDDDA
Sbjct: 3 QEFFRTLQRYYSNAYMDAMKQDAINVFLGHFQPQQGKPALWELGSDQLYDAGRHGDDDA 179
Score = 87.0 bits (214), Expect(3) = 3e-59
Identities = 36/44 (81%), Positives = 41/44 (92%)
Frame = +3
Query: 449 KKERCAESHHIYYSEHGDSFSCSNFVDLDWLSSSANSCEEDPYE 492
++ERC ES HIYYS+HGDSFSCSNFVDLDWLSSS NSCEE+P+E
Sbjct: 420 QRERCLESEHIYYSDHGDSFSCSNFVDLDWLSSSGNSCEEEPFE 551
Score = 68.6 bits (166), Expect(3) = 3e-59
Identities = 44/68 (64%), Positives = 49/68 (71%), Gaps = 1/68 (1%)
Frame = +2
Query: 383 *VIFQKIFLRWKHSS*QFYTYASFKCQGRKILKP-RFTRPIRRREQGLL*VLT*NIYY*N 441
*VIFQKI LRWKHS *+FYT S K Q KP R TR IRRR+QG L*V+T NI+Y
Sbjct: 179 *VIFQKILLRWKHS**KFYTDVSPKGQACIFSKPRRLTRSIRRRKQGSL*VITRNIHY-- 352
Query: 442 *E*HFIFK 449
*+*H IFK
Sbjct: 353 *Q*HLIFK 376
>TC222375 similar to UP|Q7XZU1 (Q7XZU1) SAC domain protein 4, partial (12%)
Length = 424
Score = 214 bits (546), Expect = 5e-56
Identities = 98/131 (74%), Positives = 112/131 (84%)
Frame = +1
Query: 187 DVDKGSFSPTVHVEYDNEDANDLERKPSDEINVSNENHSAKPPRLQNGVLRTNCIDCLDR 246
++DKGSF T + DN+D N L+RKPS E N++N NH KPP Q GVLRTNCIDCLDR
Sbjct: 31 NIDKGSFQSTRQADVDNKDGNTLQRKPSGESNLANGNHFVKPPMFQRGVLRTNCIDCLDR 210
Query: 247 TNVAQYAYGLAAIGHQLHSLGIIEHPKIDLDDPVANDLMQFYERMGDTLAHQYGGSAAHN 306
TNVAQYAYGLAA+GHQLH+LGII+HPKIDLDDP+++DLM FYERMGDTLAHQYGGSAAH
Sbjct: 211 TNVAQYAYGLAALGHQLHALGIIDHPKIDLDDPLSDDLMGFYERMGDTLAHQYGGSAAHK 390
Query: 307 KIFSARRGQWR 317
KIFS RRGQW+
Sbjct: 391 KIFSERRGQWK 423
>TC215710 weakly similar to UP|Q7XZU2 (Q7XZU2) SAC domain protein 3, partial
(3%)
Length = 992
Score = 65.1 bits (157), Expect(2) = 1e-19
Identities = 31/44 (70%), Positives = 35/44 (79%)
Frame = +1
Query: 449 KKERCAESHHIYYSEHGDSFSCSNFVDLDWLSSSANSCEEDPYE 492
++ER S HI YS HGDSFS SNFVDLDWL SS NSCEE+P+E
Sbjct: 190 QRERFFHSDHISYSGHGDSFSSSNFVDLDWL-SSGNSCEEEPFE 318
Score = 49.3 bits (116), Expect(2) = 1e-19
Identities = 29/48 (60%), Positives = 36/48 (74%), Gaps = 1/48 (2%)
Frame = +3
Query: 403 YASFKCQGRKILKPRFTRPIRRREQGLL*VLT*NI-YY*N*E*HFIFK 449
Y + K + KILK RF PIRRR+QG L*V+ *NI ++*+*E*H IFK
Sbjct: 3 YVNPKSKA*KILKARFAGPIRRRKQGFL*VIA*NINFF*D*E*HVIFK 146
>TC208487 similar to UP|Q94A27 (Q94A27) At3g14201 (At3g14201/at3g14201) (SAC
domain protein 2), partial (6%)
Length = 955
Score = 79.0 bits (193), Expect = 5e-15
Identities = 56/150 (37%), Positives = 75/150 (49%), Gaps = 9/150 (6%)
Frame = +1
Query: 352 HFQPQQDKPALWELGSDQHYDTGRIG----DDDAS*VIFQKIFLRWKHSS*QFYTYASFK 407
HFQPQQ KPALWEL SDQHY + G DD I + + S + +
Sbjct: 7 HFQPQQGKPALWELDSDQHYTVKKHGLYLADDSIRSTIKRSL------SDGNILSESDTT 168
Query: 408 CQGRKILKPRFT--RPIRRREQGLL*VLT*NIYY*N*E*---HFIFKKERCAESHHIYYS 462
+ + + + +P +R G T +IY + I+ + ES HI Y
Sbjct: 169 IRNLNVTNDQNSSEKPDKRFLSGS----TPDIYTCGSDICHCRQIYVNGQNCESDHICYD 336
Query: 463 EHGDSFSCSNFVDLDWLSSSANSCEEDPYE 492
EHGD+ CSNF+D+DWLSSS NSCEE+ E
Sbjct: 337 EHGDACDCSNFLDVDWLSSSGNSCEEELLE 426
>AW306240
Length = 402
Score = 78.6 bits (192), Expect = 6e-15
Identities = 34/53 (64%), Positives = 43/53 (80%)
Frame = +2
Query: 327 RTLQRYYSNAWMDAEKQDAINVFLGHFQPQQDKPALWELGSDQHYDTGRIGDD 379
++++RYYSNA+ D EKQDAIN+FLG+FQPQ+ KPALWEL SD + IGDD
Sbjct: 5 KSIKRYYSNAYTDGEKQDAINLFLGYFQPQEGKPALWELDSDYYLHVSGIGDD 163
>TC207010 similar to GB|AAP49840.1|31415731|AY227250 SAC domain protein 7
{Arabidopsis thaliana;} , partial (36%)
Length = 975
Score = 74.3 bits (181), Expect = 1e-13
Identities = 49/138 (35%), Positives = 66/138 (47%), Gaps = 7/138 (5%)
Frame = +3
Query: 223 NHSAKPPRLQNGVLRTNCIDCLDRTNVAQYAYGLAAIGHQLHSLGII-------EHPKID 275
N + + Q GV+RTNCIDCLDRTNV Q G + QL LG+ HP +D
Sbjct: 9 NEKGEKMKEQLGVVRTNCIDCLDRTNVTQSMIGRNMLECQLRRLGVFGAEETISTHPNLD 188
Query: 276 LDDPVANDLMQFYERMGDTLAHQYGGSAAHNKIFSARRGQWRAATQSQEFFRTLQRYYSN 335
+ + GD ++ QY G+ A F R G Q+ L RYY N
Sbjct: 189 ------ENFKILWANHGDDISIQYSGTPALKGDF-VRFGHRTIQGILQDGVNALLRYYFN 347
Query: 336 AWMDAEKQDAINVFLGHF 353
++D KQDAI++ GH+
Sbjct: 348 NFVDGTKQDAIDLLQGHY 401
>TC220765 weakly similar to UP|Q7X911 (Q7X911) SAC1-like protein AtSAC1b (SAC
domain protein 6), partial (12%)
Length = 931
Score = 58.2 bits (139), Expect = 8e-09
Identities = 37/131 (28%), Positives = 63/131 (47%), Gaps = 1/131 (0%)
Frame = +1
Query: 232 QNGVLRTNCIDCLDRTNVAQYAYGLAAIGHQLHSLGIIEHPK-IDLDDPVANDLMQFYER 290
Q G++R NCID LDRTNV Q ++ QL + + + I + + +
Sbjct: 13 QRGLVRVNCIDSLDRTNVTQCYLAQKSLNLQLQRIEALTSSECISVFEEEYGKFRILWAD 192
Query: 291 MGDTLAHQYGGSAAHNKIFSARRGQWRAATQSQEFFRTLQRYYSNAWMDAEKQDAINVFL 350
GD ++ +Y G+ A K R G+ ++ L RYY N + D +QDA+++
Sbjct: 193 QGDEISLEYAGTHA-LKGDLVRYGKQTITGMIKDGMSALSRYYLNNFQDGIRQDALDLIS 369
Query: 351 GHFQPQQDKPA 361
GH+ ++ P+
Sbjct: 370 GHYNVSRNVPS 402
>BE610467
Length = 408
Score = 48.1 bits (113), Expect = 9e-06
Identities = 37/123 (30%), Positives = 62/123 (50%), Gaps = 1/123 (0%)
Frame = +3
Query: 17 NDVETEQIV-FEDVPEGLPIKISSVIQNRGSIPLFWSQETSRLNIKPDIILSKKDQSYQA 75
N VETEQI+ F +S +Q RGSIPL W Q+ L KP L K +++ +
Sbjct: 3 NFVETEQIMQFNGYT-------ASFVQVRGSIPLLW-QQIVDLTYKPKFELLKLEEAPRV 158
Query: 76 TKLHFENLVKRYGNPIIILNLIKTHEKKPREAILRQEFANAIDFINKDLSEENRLRFLHW 135
+ HF +L ++ ++ ++L+ H + R L ++F + + N +R+LH+
Sbjct: 159 LERHFLDLREKNMGAVLAVDLVNKHGGEGR---LCEKFGDTAQHV-----ASNDVRYLHF 314
Query: 136 DLH 138
D H
Sbjct: 315 DFH 323
>TC213986 similar to UP|O23215 (O23215) Transcription initiation factor like
protein (TFIIB-related protein) (At4g37010)
(TFIIB5/pBrp), partial (22%)
Length = 414
Score = 29.3 bits (64), Expect = 4.2
Identities = 14/43 (32%), Positives = 24/43 (55%)
Frame = +2
Query: 450 KERCAESHHIYYSEHGDSFSCSNFVDLDWLSSSANSCEEDPYE 492
+ER + HH+++ D+ C DL + + N+ EEDP+E
Sbjct: 125 EERQSHPHHVFHVRAQDNPLCLVTSDLALPALNQNNDEEDPFE 253
>CD392158
Length = 704
Score = 29.3 bits (64), Expect = 4.2
Identities = 20/53 (37%), Positives = 31/53 (57%)
Frame = -2
Query: 5 LRRGVNEKGRVANDVETEQIVFEDVPEGLPIKISSVIQNRGSIPLFWSQETSR 57
L RG+ +G+V ++ +F D+ EGL IK S+V N + L +Q+TSR
Sbjct: 547 LLRGLGREGKVDEAIK----IFHDM-EGLSIKPSAVTYNAIMLGLCKAQQTSR 404
>TC228225 weakly similar to UP|Q9FFJ7 (Q9FFJ7) Similarity to C3HC4-type RING
zinc finger protein, partial (10%)
Length = 689
Score = 28.5 bits (62), Expect = 7.2
Identities = 12/27 (44%), Positives = 16/27 (58%)
Frame = +1
Query: 238 TNCIDCLDRTNVAQYAYGLAAIGHQLH 264
++C+ CLD AQ+ LAA GH H
Sbjct: 241 SHCVVCLDAFRNAQWCRKLAACGHVFH 321
>AW734844
Length = 446
Score = 28.5 bits (62), Expect = 7.2
Identities = 12/27 (44%), Positives = 16/27 (58%)
Frame = +3
Query: 238 TNCIDCLDRTNVAQYAYGLAAIGHQLH 264
++C+ CLD AQ+ LAA GH H
Sbjct: 27 SHCVVCLDAFRNAQWCRKLAACGHVFH 107
>TC234537 similar to UP|Q76DY1 (Q76DY1) AG-motif binding protein-3, partial
(13%)
Length = 562
Score = 28.5 bits (62), Expect = 7.2
Identities = 15/40 (37%), Positives = 20/40 (49%)
Frame = -1
Query: 176 PEDCLKWPSTDDVDKGSFSPTVHVEYDNEDANDLERKPSD 215
P KW S ++++ SP V VE E L +KPSD
Sbjct: 382 PMAVAKWLSLPEIEELQLSPAVTVEESVEFPVTLSKKPSD 263
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.328 0.141 0.445
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,518,211
Number of Sequences: 63676
Number of extensions: 340257
Number of successful extensions: 1791
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 1773
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1784
length of query: 492
length of database: 12,639,632
effective HSP length: 101
effective length of query: 391
effective length of database: 6,208,356
effective search space: 2427467196
effective search space used: 2427467196
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC124967.6