BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124967.5 + phase: 0 /pseudo
(233 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC216029 similar to UP|IF3B_ARATH (Q9SZA3) Eukaryotic translatio... 365 e-101
TC216030 similar to UP|IF3B_ARATH (Q9SZA3) Eukaryotic translatio... 164 2e-42
TC216031 similar to UP|IF3B_ORYSA (Q94HF1) Eukaryotic translatio... 120 4e-33
BU543986 29 2.1
TC228789 weakly similar to GB|AAO24581.1|27808602|BT003149 At3g2... 28 3.6
TC209441 UP|Q39883 (Q39883) Nodulin-26, partial (98%) 27 7.9
BQ473783 27 7.9
>TC216029 similar to UP|IF3B_ARATH (Q9SZA3) Eukaryotic translation initiation
factor 3 subunit 11 (eIF-3 p25) (eIF3k), partial (77%)
Length = 1002
Score = 365 bits (936), Expect = e-101
Identities = 188/232 (81%), Positives = 201/232 (86%), Gaps = 1/232 (0%)
Frame = +3
Query: 3 REAAAAAEKGQGGTAYTVEQLVAFNRYNPDILPDLENYVNDQVSSQTYSLESNLCLLRLY 62
R+ EK AYTVEQLVAFN YNPDILPDLENYVNDQVSSQTYSL++NLCLLRLY
Sbjct: 84 RKREMGREKETTKVAYTVEQLVAFNPYNPDILPDLENYVNDQVSSQTYSLDANLCLLRLY 263
Query: 63 QFESEKMSSQIVARILVKALMAMPAPDFSLCLFLIPERVIANGRSSSRH*L-FCLTI*RF 121
QFE EKMSSQIVARILVKALMAMPAPDFSLCLFLIPERV + + L L RF
Sbjct: 264 QFEPEKMSSQIVARILVKALMAMPAPDFSLCLFLIPERVQMEEQFKTLIVLSHYLETGRF 443
Query: 122 RQFWDEASKSRHIVEAVPGFEQAIQGYAIHVLSLTYQKIPRTVLAEAINVEGLSLDKFLE 181
RQFWDEA+KSRHIVEAVPGFEQAIQGYAIHVLSLTYQ+IPRTVLAEAIN+EGLSLDKF+E
Sbjct: 444 RQFWDEAAKSRHIVEAVPGFEQAIQGYAIHVLSLTYQRIPRTVLAEAINIEGLSLDKFIE 623
Query: 182 HQVENSGWVIEKSQGKGQLIVLPRNEFNDPILKKNTADSVPLEHVTRIFPIL 233
HQV NSGWV+EKSQG+GQL+VLPR EFNDP LKKN ADSVPLEH+TRIFPIL
Sbjct: 624 HQVANSGWVVEKSQGRGQLVVLPRTEFNDPALKKNAADSVPLEHITRIFPIL 779
>TC216030 similar to UP|IF3B_ARATH (Q9SZA3) Eukaryotic translation initiation
factor 3 subunit 11 (eIF-3 p25) (eIF3k), partial (47%)
Length = 444
Score = 164 bits (416), Expect(2) = 2e-42
Identities = 84/98 (85%), Positives = 86/98 (87%)
Frame = +1
Query: 4 EAAAAAEKGQGGTAYTVEQLVAFNRYNPDILPDLENYVNDQVSSQTYSLESNLCLLRLYQ 63
E EK AY VEQLVAFNRYNPDILPDLENYVNDQVSSQTYSL++NLCLLRLYQ
Sbjct: 79 EREMGREKETTKVAYAVEQLVAFNRYNPDILPDLENYVNDQVSSQTYSLDANLCLLRLYQ 258
Query: 64 FESEKMSSQIVARILVKALMAMPAPDFSLCLFLIPERV 101
FE EKMSSQIVARILVKALMAMPAPDFSLCLFLIPERV
Sbjct: 259 FEPEKMSSQIVARILVKALMAMPAPDFSLCLFLIPERV 372
Score = 25.0 bits (53), Expect(2) = 2e-42
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = +2
Query: 106 RSSSRH*LFCLTI*RFRQF 124
RS+SR *LFCLTI R F
Sbjct: 380 RSNSRL*LFCLTIWRQEDF 436
>TC216031 similar to UP|IF3B_ORYSA (Q94HF1) Eukaryotic translation initiation
factor 3 subunit 11 (eIF-3 p25) (eIF3k), partial (32%)
Length = 648
Score = 120 bits (302), Expect(2) = 4e-33
Identities = 57/66 (86%), Positives = 62/66 (93%)
Frame = +1
Query: 168 AINVEGLSLDKFLEHQVENSGWVIEKSQGKGQLIVLPRNEFNDPILKKNTADSVPLEHVT 227
AIN+EGLSLDKFLE+QV N+GWVIEKSQG+ QLIVLPRNEFNDP LKKN ADSVPLEH+T
Sbjct: 52 AINIEGLSLDKFLENQVANNGWVIEKSQGRAQLIVLPRNEFNDPALKKNAADSVPLEHIT 231
Query: 228 RIFPIL 233
RIFPIL
Sbjct: 232 RIFPIL 249
Score = 37.7 bits (86), Expect(2) = 4e-33
Identities = 17/17 (100%), Positives = 17/17 (100%)
Frame = +2
Query: 131 SRHIVEAVPGFEQAIQG 147
SRHIVEAVPGFEQAIQG
Sbjct: 2 SRHIVEAVPGFEQAIQG 52
>BU543986
Length = 527
Score = 28.9 bits (63), Expect = 2.1
Identities = 19/61 (31%), Positives = 30/61 (49%)
Frame = +3
Query: 144 AIQGYAIHVLSLTYQKIPRTVLAEAINVEGLSLDKFLEHQVENSGWVIEKSQGKGQLIVL 203
A G ++ ++ + P L A ++ L KF HQ ++GW + SQ K QLI +
Sbjct: 309 ASNGPGLNSIAANVES*PIIALVRAXXIDDFVL-KFFLHQ--STGWRLIMSQSKSQLIHI 479
Query: 204 P 204
P
Sbjct: 480 P 482
>TC228789 weakly similar to GB|AAO24581.1|27808602|BT003149 At3g29770
{Arabidopsis thaliana;} , partial (17%)
Length = 1034
Score = 28.1 bits (61), Expect = 3.6
Identities = 22/63 (34%), Positives = 29/63 (45%), Gaps = 1/63 (1%)
Frame = -1
Query: 164 VLAEAINVEGLSLDKFLEHQVENSGWVIEKSQGKGQLIVLP-RNEFNDPILKKNTADSVP 222
+L A ++ L KF HQ ++GW + SQ K QLI +P PI K
Sbjct: 548 ILVRAQRIDDFVL-KFFLHQ--STGWRLIMSQSKSQLIHIPILG*IRHPIFKILIGQESE 378
Query: 223 LEH 225
LEH
Sbjct: 377 LEH 369
>TC209441 UP|Q39883 (Q39883) Nodulin-26, partial (98%)
Length = 982
Score = 26.9 bits (58), Expect = 7.9
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = -1
Query: 93 CLFLIPERVIANGRSSSRH*LFCLTI*RFR 122
C+ L+ R I+NG S +RH L L++ FR
Sbjct: 130 CIRLVQLRRISNGNSRNRHFLLLLSLRLFR 41
>BQ473783
Length = 425
Score = 26.9 bits (58), Expect = 7.9
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +2
Query: 69 MSSQIVARILVKALMAMPAPDFSLCLFLIP 98
+++Q + R+++ A A DFSLC+ LIP
Sbjct: 179 IAAQPLLRLILNASAATRIRDFSLCMALIP 268
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.325 0.139 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,371,753
Number of Sequences: 63676
Number of extensions: 119531
Number of successful extensions: 611
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 608
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 610
length of query: 233
length of database: 12,639,632
effective HSP length: 94
effective length of query: 139
effective length of database: 6,654,088
effective search space: 924918232
effective search space used: 924918232
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC124967.5