Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC124967.11 + phase: 0 
         (602 letters)

Database: GMGI 
           63,676 sequences; 37,918,896 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BF425356 similar to PIR|T08400|T08 late embryonic abundant prote...    82  5e-16
TC231367 similar to PIR|T08400|T08400 late embryonic abundant pr...    73  4e-13
BG725524 similar to GP|5091614|gb|A Contains a PF|00561 alpha/be...    39  0.007
CD414656                                                               30  4.0
TC219657 weakly similar to UP|AROK_ARATH (Q9SJ05) Probable shiki...    29  6.8
TC209029 similar to GB|BAB10719.1|10177251|AB007644 receptor pro...    29  6.8
CA938791                                                               28  8.9
BG509439                                                               28  8.9
TC227639 similar to UP|E74B_DROME (P11536) Ecdysone-induced prot...    28  8.9

>BF425356 similar to PIR|T08400|T08 late embryonic abundant protein EMB8
           homolog F18B3.70 - Arabidopsis thaliana, partial (31%)
          Length = 390

 Score = 82.4 bits (202), Expect = 5e-16
 Identities = 46/130 (35%), Positives = 72/130 (55%), Gaps = 3/130 (2%)
 Frame = +1

Query: 141 ELKYQRVCLSASDGGVVSLDWPVELDLEEERGL--DSTLLIV-PGTPQGSMDDNIRVFVI 197
           +++ +R CL   DGG V+LDW       ++R L  DS LLI+ PG   GS D  +R  ++
Sbjct: 13  DVRLRRECLRTQDGGAVALDWVSG----DDRRLPPDSPLLILLPGLTGGSGDAYVRHMLV 180

Query: 198 DALKRGFFPVVMNPRGCASSPITTPRLFTAADSDDICTAITYINKARPWTTLMGVGWGYG 257
            A  +G   VV N RGC  SP+TTP+ ++A+   D+   ++++    P   +  + W  G
Sbjct: 181 RARSKGCRVVVFNSRGCGDSPVTTPQFYSASFLGDMREVVSHVTGRYPNANVYAIXWSLG 360

Query: 258 ANMLTKYLAE 267
           AN+L  YL +
Sbjct: 361 ANILVHYLGQ 390


>TC231367 similar to PIR|T08400|T08400 late embryonic abundant protein EMB8
           homolog F18B3.70 - Arabidopsis thaliana {Arabidopsis
           thaliana;} , partial (39%)
          Length = 881

 Score = 72.8 bits (177), Expect = 4e-13
 Identities = 42/132 (31%), Positives = 77/132 (57%), Gaps = 3/132 (2%)
 Frame = +3

Query: 275 TAATCIDNPFDLDEATRTFP--YHHVTDQKLTRGLINILQTNKALFQGKAKGXDVGKALL 332
           + A  + NPF+L  A   F   ++ + D+ L++ L  I   +  LF+      ++  A  
Sbjct: 6   SGAVSLCNPFNLVVADEDFRKGFNIIYDKVLSKALRKIFNKHVLLFEDIGGEYNIPLAAN 185

Query: 333 AKSVRDFEEAISMVSYGFVDIEDFYTKASTRNMIKDIKIPVLFIQSDNG-MVPAFSVPRN 391
           AKSVR+F++A++ VS+GF  ++++Y+ +S+ + IK ++ P+L IQ+ N  + P   +PR 
Sbjct: 186 AKSVREFDDALTRVSFGFKSVDEYYSNSSSSDSIKHVRTPLLCIQAANDPIAPNRGIPRE 365

Query: 392 LIAENPFTSLLL 403
            I ENP   L++
Sbjct: 366 DIEENPNCLLIV 401


>BG725524 similar to GP|5091614|gb|A Contains a PF|00561 alpha/beta hydrolase
           fold domain. {Arabidopsis thaliana}, partial (18%)
          Length = 308

 Score = 38.9 bits (89), Expect = 0.007
 Identities = 25/69 (36%), Positives = 33/69 (47%), Gaps = 3/69 (4%)
 Frame = +2

Query: 253 GWGYGANMLTKYLAEVGERTPLTAATCIDNPFDLDEATRTFPYHHVT---DQKLTRGLIN 309
           G   GAN+L KYL E GE  P+  A  + +P+DL    R     HV    D+ L  GL  
Sbjct: 8   GTRIGANILIKYLGEDGENIPVAGAVAVCSPWDLLIGDRFITRRHVQKFYDKALAIGLKG 187

Query: 310 ILQTNKALF 318
             + + A F
Sbjct: 188 YAKLHPASF 214


>CD414656 
          Length = 635

 Score = 29.6 bits (65), Expect = 4.0
 Identities = 15/31 (48%), Positives = 16/31 (51%)
 Frame = -2

Query: 12  PAKPFPSRQFRLYKRRRLKIKASFPVPPPSP 42
           P +P P R  RL  RRR     S   PPPSP
Sbjct: 619 PPRPTPPRTRRLSDRRRTPPARSLCSPPPSP 527


>TC219657 weakly similar to UP|AROK_ARATH (Q9SJ05) Probable shikimate kinase,
           chloroplast precursor  , partial (56%)
          Length = 864

 Score = 28.9 bits (63), Expect = 6.8
 Identities = 22/60 (36%), Positives = 30/60 (49%)
 Frame = -1

Query: 38  PPPSPFENLFNTLISQCSSVNSIDFIAPSLGFASGSALFFSRFKSSQNSDVGEWILFASP 97
           PPPSPF+ L     S CSS +    IA S+G  S ++L  S      N  +   + +ASP
Sbjct: 537 PPPSPFKKL-----SICSSAS----IAISVGDNSDTSLLLSFIAIFFNETLALALAYASP 385


>TC209029 similar to GB|BAB10719.1|10177251|AB007644 receptor protein
           kinase-like protein {Arabidopsis thaliana;} , partial
           (9%)
          Length = 578

 Score = 28.9 bits (63), Expect = 6.8
 Identities = 28/100 (28%), Positives = 46/100 (46%), Gaps = 12/100 (12%)
 Frame = -2

Query: 387 SVPRN-LIAENPFTSLLLCSCLSSSVMD----TDTS----ALSWCQLVTVEVAI*LSIFY 437
           S P N  ++ +  T+ +   CL SS +     T TS    +LS CQ+   +++  L  ++
Sbjct: 412 SEPSNPTLSSHETTNSIWGLCLGSSSIHLQAITSTSKSCFSLSLCQMTESKISCSLFSYF 233

Query: 438 MCN---FGMHMQWLAAVELGLLKGRHPLLTDIDLTIIPSK 474
           +CN     + ++WL    +GL   R    T   L I P K
Sbjct: 232 ICNTQDTRLLLRWLTVTSIGLRPVRSSTRTTPKL*ISPLK 113


>CA938791 
          Length = 428

 Score = 28.5 bits (62), Expect = 8.9
 Identities = 11/25 (44%), Positives = 16/25 (64%)
 Frame = +3

Query: 143 KYQRVCLSASDGGVVSLDWPVELDL 167
           KY+R   +  DGG V+LDW +  D+
Sbjct: 306 KYRRQLFTTPDGGTVALDWLLSSDV 380


>BG509439 
          Length = 441

 Score = 28.5 bits (62), Expect = 8.9
 Identities = 26/87 (29%), Positives = 38/87 (42%), Gaps = 2/87 (2%)
 Frame = -3

Query: 28  RLKIKASFPVPPPSPFENLFNTLISQCSSVNSIDFIAPSLGFASGSALFFSR--FKSSQN 85
           RL++ ++ P+P         + LI  C S      + PSL    G  LF       +  +
Sbjct: 343 RLRVFSAIPLPA--------SLLIMSCQS------LPPSLSSFPGMTLFLFE*VLSTGLS 206

Query: 86  SDVGEWILFASPTPFNRFVLLRCPSIS 112
           S+    ILFAS +    F LL  PSI+
Sbjct: 205 SNSNSIILFASSSACRLFPLLLKPSIT 125


>TC227639 similar to UP|E74B_DROME (P11536) Ecdysone-induced protein 74EF
           isoform B (ETS-related protein E74B), partial (3%)
          Length = 1387

 Score = 28.5 bits (62), Expect = 8.9
 Identities = 16/40 (40%), Positives = 22/40 (55%)
 Frame = -2

Query: 73  SALFFSRFKSSQNSDVGEWILFASPTPFNRFVLLRCPSIS 112
           +++   R   SQNS  G     ASP+PF  F L  CPS++
Sbjct: 984 NSILHDRSFISQNSSPG-----ASPSPFQNFEL*ICPSVT 880


  Database: GMGI
    Posted date:  Oct 22, 2004  4:58 PM
  Number of letters in database: 37,918,896
  Number of sequences in database:  63,676
  
Lambda     K      H
   0.320    0.136    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,178,459
Number of Sequences: 63676
Number of extensions: 361829
Number of successful extensions: 2002
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1974
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2001
length of query: 602
length of database: 12,639,632
effective HSP length: 103
effective length of query: 499
effective length of database: 6,081,004
effective search space: 3034420996
effective search space used: 3034420996
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)


Medicago: description of AC124967.11