BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124963.22 + phase: 0 /pseudo
(516 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC208741 similar to UP|Q9FN53 (Q9FN53) Folylpolyglutamate syntha... 244 1e-83
AI973619 similar to GP|17976757|em dihydrofolate synthetase {Ara... 59 7e-09
TC224658 similar to UP|METL_PEA (P49613) S-adenosylmethionine sy... 30 2.6
TC209332 similar to UP|Q9SS90 (Q9SS90) F4P13.19 protein, partial... 30 3.4
TC224576 homologue to UP|Q8W3Y4 (Q8W3Y4) S-adenosylmethionine sy... 29 5.8
TC224800 homologue to UP|PSA4_SPIOL (P52427) Proteasome subunit ... 29 5.8
TC224552 homologue to UP|METK_CATRO (Q96551) S-adenosylmethionin... 29 5.8
TC224617 homologue to UP|METK_HORVU (P50299) S-adenosylmethionin... 29 5.8
TC224596 homologue to UP|Q9AT56 (Q9AT56) S-adenosyl-L-methionine... 29 5.8
TC224789 homologue to UP|Q8W3Y4 (Q8W3Y4) S-adenosylmethionine sy... 29 5.8
BG405836 weakly similar to GP|11994216|db disease resistance com... 28 7.6
TC220538 similar to UP|Q9FHM5 (Q9FHM5) Similarity to DNA-binding... 28 9.9
>TC208741 similar to UP|Q9FN53 (Q9FN53) Folylpolyglutamate synthase-like
protein, partial (34%)
Length = 1082
Score = 244 bits (623), Expect(2) = 1e-83
Identities = 128/160 (80%), Positives = 137/160 (85%)
Frame = +1
Query: 357 AHTKESAKALMNTIKMASPKARLAFVVAMASDKDHAGFAREILSDAYVKTVILTEAAIAG 416
AHTKESAKALMNTI+MA PKA+L FVVAMASDKDH GFAREILS +V+TV+LTEAAIAG
Sbjct: 229 AHTKESAKALMNTIRMAFPKAQLVFVVAMASDKDHVGFAREILSGVHVETVVLTEAAIAG 408
Query: 417 AVTRTAPASLLRDSWIKASEELGTDICHDGMTEYRELFKEQPVSSESNLTDGKTILATES 476
VTRT PASLLRDSWIKAS ELG I HDGM EY EL KEQPV SESNL DGKT+LATES
Sbjct: 409 GVTRTTPASLLRDSWIKASGELGIGIVHDGMAEYSELLKEQPVGSESNLGDGKTVLATES 588
Query: 477 SLKDCLRMANEILNRRRDEKGVIVITGSLHIVSSVLASLA 516
LK CLR AN+IL+RR DEK VIV TGSLHI +SVLASLA
Sbjct: 589 CLKGCLRSANQILSRRGDEKRVIVFTGSLHIAASVLASLA 708
Score = 84.3 bits (207), Expect(2) = 1e-83
Identities = 49/76 (64%), Positives = 58/76 (75%)
Frame = +2
Query: 278 VSCMI*SCKCLVLTNFKMQQLLLV*HSVFVI*VDGEFLMNL*GVG*SVHTSLEGVSS*NL 337
VS M+*SCKCL +TNFKMQ LLLV H F I* DG FL+NL G+ * +HT LEGV+ *+L
Sbjct: 2 VSYMM*SCKCLEITNFKMQLLLLVWHLFFAI*-DGVFLINLLGMV*RIHTFLEGVNF*HL 178
Query: 338 KKPRPWDFLEQQYCLI 353
KK R D L+QQYCL+
Sbjct: 179 KKLRY*DLLDQQYCLM 226
>AI973619 similar to GP|17976757|em dihydrofolate synthetase {Arabidopsis
thaliana}, partial (19%)
Length = 407
Score = 58.5 bits (140), Expect = 7e-09
Identities = 31/70 (44%), Positives = 42/70 (59%)
Frame = +3
Query: 132 AFILFADEKVDIAVIEAGLGGARDATNIISSSGLAAAVITTVGEEHLAALGGSLESIAMA 191
AF +F E+VD A+IE GLGG D+TN+I + IT++G +H LG ++ IA
Sbjct: 3 AFKIFISEQVDAAIIEVGLGGKEDSTNVIKEPTVCG--ITSLGMDHTEILGDTIGQIASH 176
Query: 192 KAGIIKQGCP 201
KAGI K P
Sbjct: 177 KAGIFKPKVP 206
>TC224658 similar to UP|METL_PEA (P49613) S-adenosylmethionine synthetase 2
(Methionine adenosyltransferase 2) (AdoMet synthetase 2)
, partial (49%)
Length = 781
Score = 30.0 bits (66), Expect = 2.6
Identities = 16/57 (28%), Positives = 25/57 (43%)
Frame = +2
Query: 219 KAVNMDSPVVSASDSGNNLAVKSFSILNGKPCQICDIEIQTVKDLKLAMDPDPRCAC 275
K ++D ++ + S S+ G P ++CD V D L DPD + AC
Sbjct: 191 KCSSLDFVLLEVKMAQETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVAC 361
>TC209332 similar to UP|Q9SS90 (Q9SS90) F4P13.19 protein, partial (11%)
Length = 692
Score = 29.6 bits (65), Expect = 3.4
Identities = 19/85 (22%), Positives = 33/85 (38%)
Frame = +3
Query: 217 REKAVNMDSPVVSASDSGNNLAVKSFSILNGKPCQICDIEIQTVKDLKLAMDPDPRCACG 276
R + +P ++ DS + S S+ + K C IC + KD+ C CG
Sbjct: 126 RSNSFQQRTPTHTSYDSSVHTETSSSSLYDNKVCPIC---LTNAKDMAFGCGHQTCCECG 296
Query: 277 PVSCMI*SCKCLVLTNFKMQQLLLV 301
C+ + T ++ L L+
Sbjct: 297 EDLQFCPICRSTIHTRIRLY*LALM 371
>TC224576 homologue to UP|Q8W3Y4 (Q8W3Y4) S-adenosylmethionine synthetase,
complete
Length = 1476
Score = 28.9 bits (63), Expect = 5.8
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +3
Query: 241 SFSILNGKPCQICDIEIQTVKDLKLAMDPDPRCAC 275
S S+ G P ++CD V D L DPD + AC
Sbjct: 114 SESVNEGHPDKLCDQISDAVLDACLEQDPDSKVAC 218
>TC224800 homologue to UP|PSA4_SPIOL (P52427) Proteasome subunit alpha type 4
(20S proteasome alpha subunit C) (20S proteasome subunit
alpha-3) (Proteasome 27 kDa subunit) , partial (98%)
Length = 1303
Score = 28.9 bits (63), Expect = 5.8
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +2
Query: 241 SFSILNGKPCQICDIEIQTVKDLKLAMDPDPRCAC 275
S S+ G P ++CD V D L DPD + AC
Sbjct: 1148 SESVNEGHPDKLCDQISDAVLDACLEQDPDSKVAC 1252
>TC224552 homologue to UP|METK_CATRO (Q96551) S-adenosylmethionine synthetase
1 (Methionine adenosyltransferase 1) (AdoMet synthetase
1) , complete
Length = 1784
Score = 28.9 bits (63), Expect = 5.8
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +2
Query: 241 SFSILNGKPCQICDIEIQTVKDLKLAMDPDPRCAC 275
S S+ G P ++CD V D L DPD + AC
Sbjct: 149 SESVNEGHPDKLCDQISDAVLDACLEQDPDSKVAC 253
>TC224617 homologue to UP|METK_HORVU (P50299) S-adenosylmethionine synthetase
1 (Methionine adenosyltransferase 1) (AdoMet synthetase
1) , partial (31%)
Length = 590
Score = 28.9 bits (63), Expect = 5.8
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +2
Query: 241 SFSILNGKPCQICDIEIQTVKDLKLAMDPDPRCAC 275
S S+ G P ++CD V D L DPD + AC
Sbjct: 245 SESVNEGHPDKLCDQISDAVLDACLEQDPDSKVAC 349
>TC224596 homologue to UP|Q9AT56 (Q9AT56) S-adenosyl-L-methionine synthetase
, complete
Length = 1597
Score = 28.9 bits (63), Expect = 5.8
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +1
Query: 241 SFSILNGKPCQICDIEIQTVKDLKLAMDPDPRCAC 275
S S+ G P ++CD V D L DPD + AC
Sbjct: 145 SESVNEGHPDKLCDQISDAVLDACLEQDPDSKVAC 249
>TC224789 homologue to UP|Q8W3Y4 (Q8W3Y4) S-adenosylmethionine synthetase,
complete
Length = 1736
Score = 28.9 bits (63), Expect = 5.8
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +1
Query: 241 SFSILNGKPCQICDIEIQTVKDLKLAMDPDPRCAC 275
S S+ G P ++CD V D L DPD + AC
Sbjct: 253 SESVNEGHPDKLCDQISDAVLDACLEQDPDSKVAC 357
>BG405836 weakly similar to GP|11994216|db disease resistance comples protein
{Arabidopsis thaliana}, partial (2%)
Length = 410
Score = 28.5 bits (62), Expect = 7.6
Identities = 12/29 (41%), Positives = 21/29 (72%)
Frame = +1
Query: 210 PHIEYIIREKAVNMDSPVVSASDSGNNLA 238
P+++ ++ K NM+S +VS S+S NNL+
Sbjct: 13 PNLKRLVLVKCENMESLLVSLSESSNNLS 99
>TC220538 similar to UP|Q9FHM5 (Q9FHM5) Similarity to DNA-binding protein,
partial (41%)
Length = 825
Score = 28.1 bits (61), Expect = 9.9
Identities = 42/174 (24%), Positives = 71/174 (40%), Gaps = 11/174 (6%)
Frame = +1
Query: 70 YSVGCYSSPHIQTIRERILGRSGDPVSAKLLNNLFHRIKQDLDQA--IKEENGCISHFEV 127
YSVG +PHI T+ E G+ V+ K+++ Q QA I NG IS+ +
Sbjct: 313 YSVGANFTPHILTVNE------GEDVTMKIMS-----FSQQGCQAICILSANGTISNVTL 459
Query: 128 F--TAMAFILFADEKVDIAVIEAGLGGARDATNIISSSGLAAAVITTVGEEHLAALGGSL 185
T+ L + + +I + + S G++ ++ G +GG L
Sbjct: 460 RQPTSSGGTLTYEGRFEILSLSGSYITTENGLTKSRSGGMSISLAAPDGR----VMGGGL 627
Query: 186 ESIAMAKAGIIKQGCPLVLGG-------PFLPHIEYIIREKAVNMDSPVVSASD 232
+ +A AG ++ L G P P +E+II A +P +A +
Sbjct: 628 AGLLVA-AGPVQVVVASFLPGHQLEQQKPKKPRVEHIISMAAPMHVNPTSAAEE 786
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.327 0.140 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,485,677
Number of Sequences: 63676
Number of extensions: 235961
Number of successful extensions: 1329
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 1326
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1327
length of query: 516
length of database: 12,639,632
effective HSP length: 101
effective length of query: 415
effective length of database: 6,208,356
effective search space: 2576467740
effective search space used: 2576467740
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC124963.22