BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124217.9 + phase: 0 /pseudo
(145 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NP395548 reverse transcriptase [Glycine max] 54 3e-08
NP395547 reverse transcriptase [Glycine max] 49 8e-07
TC214753 UP|Q39843 (Q39843) Ascorbate peroxidase 2 , partial (45%) 30 0.31
TC214752 UP|Q76LA6 (Q76LA6) Cytosolic ascorbate peroxidase 2, co... 30 0.31
BU764155 weakly similar to GP|6523547|emb| hydroxyproline-rich g... 30 0.31
TC214824 UP|Q9MAZ9 (Q9MAZ9) Nonclathrin coat protein zeta1-COP, ... 28 1.5
TC210254 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F1... 27 2.6
TC229793 homologue to UP|FD6C_SOYBN (P48628) Omega-6 fatty acid ... 27 2.6
TC231215 similar to UP|Q6L4C3 (Q6L4C3) Expressed protein, partia... 27 2.6
TC229783 weakly similar to UP|Q94BV9 (Q94BV9) At2g03480/T4M8.9, ... 27 2.6
TC209105 27 2.6
TC230213 weakly similar to UP|Q9ZTM8 (Q9ZTM8) PGPS/D12, partial ... 27 3.4
BU549688 27 3.4
TC208041 27 3.4
TC209420 weakly similar to UP|Q9LVI6 (Q9LVI6) Probable receptor-... 27 3.4
TC233919 similar to UP|Q8VXV2 (Q8VXV2) At2g5740/F3N11.19, partia... 27 4.5
TC228879 similar to UP|Q6NPN9 (Q6NPN9) At1g76260, partial (30%) 27 4.5
TC205625 similar to GB|CAD90763.1|47825426|ATH560718 lumenal pro... 27 4.5
TC229051 similar to UP|Q9LPZ7 (Q9LPZ7) T23J18.4, partial (13%) 26 5.9
TC213543 26 5.9
>NP395548 reverse transcriptase [Glycine max]
Length = 762
Score = 53.5 bits (127), Expect = 3e-08
Identities = 42/127 (33%), Positives = 57/127 (44%)
Frame = +2
Query: 10 RPEGWIIFPCPSWTRCLRG*RDKPFIAFWMGILGTTKLRCL*RIKRRQLSFSHLVCSHFG 69
+P+G I PSW RC R +D IA WM L +L RI+RR S + LV
Sbjct: 194 KPQGKTISLYPSWIRCWRDLQDTLIIASWMHTLDIIRLL*TPRIRRRWPSHALLVSLPID 373
Query: 70 GCLSVCVMRLLLSKGACLSSSLTCLRRTYRYLWRTFLFWVSPLIIVYIT*MMFSRDATKL 129
G C M L SK AC R+ +Y W TF + +V + + +DA K
Sbjct: 374 GFHLGCAMHLPHSKCACWPFLQI*WRKASKYSWMTFQYLCPH*KVV*RSWRWYYKDAWKQ 553
Query: 130 ILF*TGK 136
+* G+
Sbjct: 554 T*Y*IGR 574
>NP395547 reverse transcriptase [Glycine max]
Length = 762
Score = 48.9 bits (115), Expect = 8e-07
Identities = 42/136 (30%), Positives = 56/136 (40%)
Frame = +2
Query: 9 IRPEGWIIFPCPSWTRCLRG*RDKPFIAFWMGILGTTKLRCL*RIKRRQLSFSHLVCSHF 68
++P+ I PSW +CLR + P W T +L+ + RIK++QL V
Sbjct: 191 MKPQEKTITHFPSWIKCLRDLQGNPSTVSWTDTQVTIRLQWILRIKKKQLLHVLSVFLLI 370
Query: 69 GGCLSVCVMRLLLSKGACLSSSLTCLRRTYRYLWRTFLFWVSPLIIVYIT*MMFSRDATK 128
C SV VM LLL + +T R + LW F V L I *
Sbjct: 371 AACRSVYVMPLLLFRDV*WQFLMTW*RNVLKSLWMIFRSLVHLLEIA*QI*RKCYNVVKN 550
Query: 129 LILF*TGKMSLYGYRK 144
LI TGK Y+K
Sbjct: 551 LIWCLTGKNVTLWYKK 598
>TC214753 UP|Q39843 (Q39843) Ascorbate peroxidase 2 , partial (45%)
Length = 766
Score = 30.4 bits (67), Expect = 0.31
Identities = 24/80 (30%), Positives = 42/80 (52%), Gaps = 9/80 (11%)
Frame = +1
Query: 53 IKRRQLSFSHLVCSHFGGCLSVCVMRLLLSKGAC-LSSSLTCLRRTYRYLWRTFL----- 106
+ R++ SFS+L+ HF CL++C LL + + SSL LR T +L L
Sbjct: 175 VVRKKASFSYLLTRHF--CLTLCSALLLRNMHRTKMPSSLITLRLTKSFLSLGLLKPKQR 348
Query: 107 ---FWVSPLIIVYIT*MMFS 123
W + I++++*+++S
Sbjct: 349 FGDCWRGTVPIIFLS*ILWS 408
>TC214752 UP|Q76LA6 (Q76LA6) Cytosolic ascorbate peroxidase 2, complete
Length = 1155
Score = 30.4 bits (67), Expect = 0.31
Identities = 24/80 (30%), Positives = 42/80 (52%), Gaps = 9/80 (11%)
Frame = +2
Query: 53 IKRRQLSFSHLVCSHFGGCLSVCVMRLLLSKGAC-LSSSLTCLRRTYRYLWRTFL----- 106
+ R++ SFS+L+ HF CL++C LL + + SSL LR T +L L
Sbjct: 692 VVRKKASFSYLLTRHF--CLTLCSALLLRNMHRTKMPSSLITLRLTKSFLSLGLLKPKQR 865
Query: 107 ---FWVSPLIIVYIT*MMFS 123
W + I++++*+++S
Sbjct: 866 FGDCWRGTVPIIFLS*ILWS 925
>BU764155 weakly similar to GP|6523547|emb| hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (11%)
Length = 434
Score = 30.4 bits (67), Expect = 0.31
Identities = 17/46 (36%), Positives = 22/46 (46%), Gaps = 1/46 (2%)
Frame = +3
Query: 65 CSHFGGCLSVCVMRLLLSKGAC-LSSSLTCLRRTYRYLWRTFLFWV 109
C H G C C + C L SL CL+R++R W T L W+
Sbjct: 87 CRHHGLCHRHCDHQYQYHHHHCSLRCSLRCLQRSHRSHWITNLQWI 224
>TC214824 UP|Q9MAZ9 (Q9MAZ9) Nonclathrin coat protein zeta1-COP, partial (68%)
Length = 1686
Score = 28.1 bits (61), Expect = 1.5
Identities = 25/78 (32%), Positives = 34/78 (43%), Gaps = 5/78 (6%)
Frame = +2
Query: 18 PCPSWTRCLRG*RDKPFIAFWMGILGTTKLRCL*RIKRRQLSFSHLV--CSHFGGCLSVC 75
PC W + G I +W +LG R LS + LV C + +S+C
Sbjct: 1457 PC*RWKCEMEGIE----IGYWKLLLG-----------ERDLSLARLVSRCGYNA*TMSLC 1591
Query: 76 VMRLL---LSKGACLSSS 90
V+RLL LS G C S+
Sbjct: 1592 VLRLLSKVLSSG*CCHSN 1645
>TC210254 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17A9_9
{Arabidopsis thaliana;} , partial (6%)
Length = 814
Score = 27.3 bits (59), Expect = 2.6
Identities = 23/73 (31%), Positives = 35/73 (47%), Gaps = 3/73 (4%)
Frame = -3
Query: 61 SHLVCSHFGGCLSV--CVMRLLLSKGACLSSSLTCLRRTYRYLWRTFLFWVSPLIIVYIT 118
+ + CSH C+S+ C + L L C R Y L FL W SPL +++ +
Sbjct: 548 NQIPCSHVLKCISILYCCWK*LRDVHFDSKRFLCCELRYYESL-HVFLLWPSPLHLLH*S 372
Query: 119 *-MMFSRDATKLI 130
* ++ S D+T I
Sbjct: 371 *RLIMSIDSTCFI 333
>TC229793 homologue to UP|FD6C_SOYBN (P48628) Omega-6 fatty acid desaturase,
chloroplast precursor , complete
Length = 1719
Score = 27.3 bits (59), Expect = 2.6
Identities = 16/44 (36%), Positives = 23/44 (51%), Gaps = 5/44 (11%)
Frame = -3
Query: 75 CVMRLLLSKGA-CLSSSLTCLRRTY----RYLWRTFLFWVSPLI 113
C + LS GA C + C+ T ++L R LFW+SPL+
Sbjct: 331 CFLYSALSTGAGCTGIATACMTVTLFLVTKFL*RLNLFWISPLL 200
>TC231215 similar to UP|Q6L4C3 (Q6L4C3) Expressed protein, partial (97%)
Length = 920
Score = 27.3 bits (59), Expect = 2.6
Identities = 18/59 (30%), Positives = 30/59 (50%), Gaps = 15/59 (25%)
Frame = -1
Query: 58 LSFSH----LVCSHFGGCL----------SVCVMRLLLSKGACLSSSLT-CLRRTYRYL 101
+SFS+ + S GGC+ S+C++ LLS L SS++ C R +R++
Sbjct: 563 ISFSNSEITFIVSDLGGCMAINFLIASHSSLCLLSTLLSSSCFLRSSMSDCASRIWRFI 387
>TC229783 weakly similar to UP|Q94BV9 (Q94BV9) At2g03480/T4M8.9, partial
(40%)
Length = 866
Score = 27.3 bits (59), Expect = 2.6
Identities = 11/16 (68%), Positives = 13/16 (80%)
Frame = +2
Query: 1 MCIDYRRLIRPEGWII 16
M I+ RL+RPEGWII
Sbjct: 323 MFIEIDRLLRPEGWII 370
>TC209105
Length = 1050
Score = 27.3 bits (59), Expect = 2.6
Identities = 13/22 (59%), Positives = 15/22 (68%), Gaps = 2/22 (9%)
Frame = -2
Query: 57 QLSFSH--LVCSHFGGCLSVCV 76
Q SF+ LVCSHFG C +CV
Sbjct: 380 QYSFAS*LLVCSHFGYCPVICV 315
>TC230213 weakly similar to UP|Q9ZTM8 (Q9ZTM8) PGPS/D12, partial (16%)
Length = 767
Score = 26.9 bits (58), Expect = 3.4
Identities = 14/30 (46%), Positives = 18/30 (59%), Gaps = 2/30 (6%)
Frame = +3
Query: 65 CSHFGGCLSVCVMRLLLSKG--ACLSSSLT 92
C+H L C+ RLLL G AC ++SLT
Sbjct: 93 CAHIEISLKKCLRRLLLDHGRVACAAASLT 182
>BU549688
Length = 688
Score = 26.9 bits (58), Expect = 3.4
Identities = 16/48 (33%), Positives = 26/48 (53%)
Frame = -2
Query: 70 GCLSVCVMRLLLSKGACLSSSLTCLRRTYRYLWRTFLFWVSPLIIVYI 117
GCL + ++RL G ++L C +++YLW F F + I +YI
Sbjct: 318 GCLHI-IIRL----GGFWRAALMCFVISFQYLWYWFDFIIYIYIYIYI 190
>TC208041
Length = 698
Score = 26.9 bits (58), Expect = 3.4
Identities = 12/48 (25%), Positives = 25/48 (52%)
Frame = +1
Query: 48 RCL*RIKRRQLSFSHLVCSHFGGCLSVCVMRLLLSKGACLSSSLTCLR 95
+C+ R ++ ++H + + + + V V + ++ SSSLTC R
Sbjct: 199 QCIERFRKYDHDYAHRLLAKYVASIEVFVREITVNDEIIKSSSLTCYR 342
>TC209420 weakly similar to UP|Q9LVI6 (Q9LVI6) Probable receptor-like protein
kinase protein (AT3g17840/MEB5_6), partial (35%)
Length = 1157
Score = 26.9 bits (58), Expect = 3.4
Identities = 17/43 (39%), Positives = 20/43 (45%)
Frame = +2
Query: 68 FGGCLSVCVMRLLLSKGACLSSSLTCLRRTYRYLWRTFLFWVS 110
+ GC VCV LLL + +S CLR T LW W S
Sbjct: 263 YSGCACVCVSLLLLPQ-----NSQCCLRST---LWWRHWLWCS 367
>TC233919 similar to UP|Q8VXV2 (Q8VXV2) At2g5740/F3N11.19, partial (5%)
Length = 414
Score = 26.6 bits (57), Expect = 4.5
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +3
Query: 74 VCVMRLLLSKGACLSSSLTCLRRTYRYLWRTFLF 107
VC + G SSSL C R +RYL+R F +
Sbjct: 219 VCAFPFAIPFGCFSSSSLIC-RIQFRYLYRAFAY 317
>TC228879 similar to UP|Q6NPN9 (Q6NPN9) At1g76260, partial (30%)
Length = 742
Score = 26.6 bits (57), Expect = 4.5
Identities = 10/22 (45%), Positives = 13/22 (58%), Gaps = 8/22 (36%)
Frame = +3
Query: 13 GWIIF--------PCPSWTRCL 26
GWI+F PC SW+RC+
Sbjct: 645 GWIVFLAYSLIRHPCNSWSRCI 710
>TC205625 similar to GB|CAD90763.1|47825426|ATH560718 lumenal protein subunit
of photosystem II {Arabidopsis thaliana;} , partial
(48%)
Length = 674
Score = 26.6 bits (57), Expect = 4.5
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +3
Query: 61 SHLVCSHFGGCLSVCVMRLLLSKGACLSSS 90
SH +C +G L +C+ ++ K C S+S
Sbjct: 363 SHCICCSYGTVLCLCIQKIR*YK*ICFSNS 452
>TC229051 similar to UP|Q9LPZ7 (Q9LPZ7) T23J18.4, partial (13%)
Length = 961
Score = 26.2 bits (56), Expect = 5.9
Identities = 15/43 (34%), Positives = 21/43 (47%)
Frame = +1
Query: 68 FGGCLSVCVMRLLLSKGACLSSSLTCLRRTYRYLWRTFLFWVS 110
FG C + LL+ GA L+ + R + YL+ T F VS
Sbjct: 361 FGSCYIQAAIYFLLAVGAFLNFIAFAVTRRHCYLYLTVAFVVS 489
>TC213543
Length = 463
Score = 26.2 bits (56), Expect = 5.9
Identities = 17/52 (32%), Positives = 27/52 (51%), Gaps = 2/52 (3%)
Frame = +2
Query: 57 QLSFSHLVCSHFGGCLSVCVMRLL-LSKGACLSSSLTCLRRTYRY-LWRTFL 106
QLSF H+ CS F + + + L LS+ L+S++ + + LW FL
Sbjct: 218 QLSFPHIHCSFFTASMLIIAVSLPGLSQQPTLNSTIATVSELLKLRLWCNFL 373
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.349 0.155 0.577
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,562,473
Number of Sequences: 63676
Number of extensions: 178890
Number of successful extensions: 1991
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 1979
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1991
length of query: 145
length of database: 12,639,632
effective HSP length: 88
effective length of query: 57
effective length of database: 7,036,144
effective search space: 401060208
effective search space used: 401060208
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC124217.9