BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124216.5 - phase: 0
(340 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC228393 similar to GB|AAR25825.1|38639365|AY380572 basic pentac... 336 1e-92
TC228406 similar to UP|Q6U1M9 (Q6U1M9) Basic pentacysteine 4, pa... 308 2e-84
TC217888 homologue to GB|AAR25825.1|38639365|AY380572 basic pent... 233 1e-61
TC211060 similar to UP|Q6U1M9 (Q6U1M9) Basic pentacysteine 4, pa... 189 1e-48
TC227440 UP|Q8LKV1 (Q8LKV1) GAGA-binding protein, complete 152 3e-37
TC208974 homologue to UP|Q8LKV1 (Q8LKV1) GAGA-binding protein, p... 151 5e-37
TC228405 homologue to UP|Q7ZXC0 (Q7ZXC0) Apacd-prov protein (Fra... 132 2e-31
TC220483 similar to UP|Q9LDE2 (Q9LDE2) F10B6.5 (T5E21.17) (At1g1... 125 4e-29
TC217887 GB|AAR25825.1|38639365|AY380572 basic pentacysteine 6 {... 91 1e-18
AW099231 similar to GP|20530716|gb| GAGA-binding protein {Glycin... 80 1e-15
BF426044 similar to SP|O49203|NDK3_ Nucleoside diphosphate kinas... 31 0.70
TC214278 similar to UP|P93704 (P93704) HMG-1, partial (98%) 31 0.70
TC216885 30 1.2
CF921606 30 1.2
BM143757 GP|20530716|gb GAGA-binding protein {Glycine max}, part... 30 1.6
TC217618 similar to GB|AAO64868.1|29028978|BT005933 At5g50860 {A... 30 1.6
BE331076 weakly similar to GP|8131699|dbj cytoplasmic ribosomal ... 30 2.0
TC231428 UP|Q8S901 (Q8S901) Syringolide-induced protein 14-1-1, ... 28 4.6
BI784937 28 6.0
TC231082 weakly similar to UP|O83031 (O83031) Orf509e (Fragment)... 28 6.0
>TC228393 similar to GB|AAR25825.1|38639365|AY380572 basic pentacysteine 6
{Arabidopsis thaliana;} , partial (32%)
Length = 924
Score = 336 bits (861), Expect = 1e-92
Identities = 176/232 (75%), Positives = 198/232 (84%), Gaps = 5/232 (2%)
Frame = +2
Query: 1 MDD---RENGRHKA-DQYKSAQGQWLMQQHQHQHPSMKQIMSIMAERDAAIQERNLALSE 56
MDD RENGRHKA DQYKSAQGQWLMQ HQ PSMKQIM+IMAERDAAIQERNLA+SE
Sbjct: 248 MDDAGHRENGRHKAADQYKSAQGQWLMQ---HQ-PSMKQIMAIMAERDAAIQERNLAISE 415
Query: 57 KKAALAERDMAFLQRDTAIAERNNALMERDNAIATLQFRENALANGGMSSCPPGCQISRG 116
KKAA AERDMAF+QRD AIAERNNA++ERDNAIATLQ+RE +L++G M SCPPGCQISRG
Sbjct: 416 KKAAYAERDMAFMQRDAAIAERNNAILERDNAIATLQYRETSLSSGSMPSCPPGCQISRG 595
Query: 117 VKHIHHLPQQVNHLPNMGDSSYGTRELHTTDALPAAPVSLEVGKPPRRAKRPKESKSDSP 176
VKHIHH QQV+H+PNMGD+SY TRE+HTT+ALPAAP+ E GK PRRAKRPKE KS SP
Sbjct: 596 VKHIHHPQQQVHHIPNMGDASYNTREMHTTEALPAAPIPSEAGK-PRRAKRPKEPKSASP 772
Query: 177 NKKTPK-SRKVKKEGDDLNKTMFANNKELEWKSSEEIINGDDDLNKQLAISK 227
NKKT K ++KVKKE +DLNK MF K EWKS +E++NG DDLNKQL +SK
Sbjct: 773 NKKTSKPAKKVKKESEDLNKVMF--GKSHEWKSGQEMVNGGDDLNKQLTVSK 922
>TC228406 similar to UP|Q6U1M9 (Q6U1M9) Basic pentacysteine 4, partial (52%)
Length = 1233
Score = 308 bits (790), Expect = 2e-84
Identities = 171/350 (48%), Positives = 222/350 (62%), Gaps = 10/350 (2%)
Frame = +2
Query: 1 MDD---RENGRHKADQYKSAQGQWLMQ-QHQHQHPSM----KQIMSIMAERDAAIQERNL 52
MDD EN RHK + Y+ A+ W QHQ + P+ K+I SIMAER AAI E L
Sbjct: 170 MDDGHQHENSRHKMEFYRGARSLWNTDSQHQVKEPNALVMNKKIRSIMAERQAAILEIEL 349
Query: 53 --ALSEKKAALAERDMAFLQRDTAIAERNNALMERDNAIATLQFRENALANGGMSSCPPG 110
A+SEK ALA RD A QRD A+A+R+NAL+ERDNA+A LQ R N++ + P G
Sbjct: 350 ETAISEKNEALAARDAAIQQRDEALAQRDNALLERDNALAALQSRNNSV------NFPFG 511
Query: 111 CQISRGVKHIHHLPQQVNHLPNMGDSSYGTRELHTTDALPAAPVSLEVGKPPRRAKRPKE 170
I G K +HH NHL NM +++Y T+++ DA P + E +AKR K+
Sbjct: 512 GGIQCGSKRMHH---SSNHLSNMTEAAYSTKDMIIRDASPVTVIPSEAVNS-HQAKRTKQ 679
Query: 171 SKSDSPNKKTPKSRKVKKEGDDLNKTMFANNKELEWKSSEEIINGDDDLNKQLAISKADW 230
+K + P KVKK G+DLN+ + ++ +++W
Sbjct: 680 NKVINSKASKPPC-KVKKMGEDLNRQASSEGTKI----------------------RSEW 790
Query: 231 KPQDLALNQVAYDDSTMPAPACSCTGVLRQCYKWGNGGWQSACCTTTLSVYPLPAVPNKR 290
QD+ LN VA+D++ MP P C+CTG+ RQCYKWGNGGWQS+CCTTTLS+YPLP +PNKR
Sbjct: 791 DKQDVGLNLVAFDETIMPVPVCTCTGIPRQCYKWGNGGWQSSCCTTTLSMYPLPQLPNKR 970
Query: 291 HARVGGRKMSGSAFNKLLSRLAAEGHDLSHPVDLKDHWAKHGTNRYITIK 340
HAR+GGRKMSGS F +LLSRL +EGHDLS P+DLK++WA+HGTNRYITIK
Sbjct: 971 HARIGGRKMSGSVFTRLLSRLVSEGHDLSIPLDLKEYWARHGTNRYITIK 1120
>TC217888 homologue to GB|AAR25825.1|38639365|AY380572 basic pentacysteine 6
{Arabidopsis thaliana;} , partial (31%)
Length = 691
Score = 233 bits (593), Expect = 1e-61
Identities = 106/113 (93%), Positives = 109/113 (95%)
Frame = +2
Query: 228 ADWKPQDLALNQVAYDDSTMPAPACSCTGVLRQCYKWGNGGWQSACCTTTLSVYPLPAVP 287
ADWK QDL LNQVAYD+STMPAP CSCTGVLRQCYKWGNGGWQSACCTTTLS+YPLPAVP
Sbjct: 2 ADWKGQDLGLNQVAYDESTMPAPVCSCTGVLRQCYKWGNGGWQSACCTTTLSMYPLPAVP 181
Query: 288 NKRHARVGGRKMSGSAFNKLLSRLAAEGHDLSHPVDLKDHWAKHGTNRYITIK 340
NKRHARVGGRKMSGSAFNKLLSRLAAE HDLS+PVDLKDHWAKHGTNRYITIK
Sbjct: 182 NKRHARVGGRKMSGSAFNKLLSRLAAESHDLSNPVDLKDHWAKHGTNRYITIK 340
>TC211060 similar to UP|Q6U1M9 (Q6U1M9) Basic pentacysteine 4, partial (40%)
Length = 702
Score = 189 bits (481), Expect = 1e-48
Identities = 80/114 (70%), Positives = 99/114 (86%)
Frame = +2
Query: 227 KADWKPQDLALNQVAYDDSTMPAPACSCTGVLRQCYKWGNGGWQSACCTTTLSVYPLPAV 286
+++W QD+ +N VA+D++ M P C+CTGV RQCYKWGNGGWQS+CCTTT S+YPLP +
Sbjct: 14 RSEWDRQDVGVNLVAFDETIMLXPVCTCTGVPRQCYKWGNGGWQSSCCTTTXSMYPLPQL 193
Query: 287 PNKRHARVGGRKMSGSAFNKLLSRLAAEGHDLSHPVDLKDHWAKHGTNRYITIK 340
PNKRHAR+GGRKMSGS F +LLSRL +EGHDLS P+DLK++WA+HGTNRYITIK
Sbjct: 194 PNKRHARIGGRKMSGSVFTRLLSRLVSEGHDLSIPLDLKEYWARHGTNRYITIK 355
>TC227440 UP|Q8LKV1 (Q8LKV1) GAGA-binding protein, complete
Length = 1305
Score = 152 bits (383), Expect = 3e-37
Identities = 82/228 (35%), Positives = 126/228 (54%)
Frame = +1
Query: 113 ISRGVKHIHHLPQQVNHLPNMGDSSYGTRELHTTDALPAAPVSLEVGKPPRRAKRPKESK 172
IS+ K+++ +P N+ SS ++ LP +E + ++ +
Sbjct: 466 ISQRDKYMNMIPTNHNYGGIPETSSAHQIQMIQAPELPKEEKPVEEAPVIEKENGTRKKR 645
Query: 173 SDSPNKKTPKSRKVKKEGDDLNKTMFANNKELEWKSSEEIINGDDDLNKQLAISKADWKP 232
S K+PK++K K+ G K D+ + ++ K
Sbjct: 646 QSSKVPKSPKAKKPKR-GPRAPK---------------------DESAPAVQRARIPKKT 759
Query: 233 QDLALNQVAYDDSTMPAPACSCTGVLRQCYKWGNGGWQSACCTTTLSVYPLPAVPNKRHA 292
++ +N + D S++P P CSCTG +QCYKWG+GGWQSACCTT +SVYPLP +R A
Sbjct: 760 TEIVINGIDMDISSIPIPVCSCTGAPQQCYKWGSGGWQSACCTTGMSVYPLPMSTKRRGA 939
Query: 293 RVGGRKMSGSAFNKLLSRLAAEGHDLSHPVDLKDHWAKHGTNRYITIK 340
R+ GRKMS AF K+L +LAAEG++ S+P+DL+ +WAKHGTN+++TI+
Sbjct: 940 RIAGRKMSIGAFKKVLEKLAAEGYNFSNPIDLRTYWAKHGTNKFVTIR 1083
>TC208974 homologue to UP|Q8LKV1 (Q8LKV1) GAGA-binding protein, partial (54%)
Length = 789
Score = 151 bits (381), Expect = 5e-37
Identities = 63/110 (57%), Positives = 86/110 (77%)
Frame = +3
Query: 231 KPQDLALNQVAYDDSTMPAPACSCTGVLRQCYKWGNGGWQSACCTTTLSVYPLPAVPNKR 290
K ++ +N + D S++P P CSCTG +QCYKWG+GGWQSACCTT +SVYPLP +R
Sbjct: 171 KTTEIVINGIDMDISSIPIPVCSCTGAHQQCYKWGSGGWQSACCTTGMSVYPLPMSTKRR 350
Query: 291 HARVGGRKMSGSAFNKLLSRLAAEGHDLSHPVDLKDHWAKHGTNRYITIK 340
AR+ GRKMS AF K+L +LAAEG++ S+P+DL+ +WAKHGTN+++TI+
Sbjct: 351 GARIAGRKMSIGAFKKVLEKLAAEGYNFSNPIDLRTYWAKHGTNKFVTIR 500
>TC228405 homologue to UP|Q7ZXC0 (Q7ZXC0) Apacd-prov protein (Fragment),
partial (5%)
Length = 792
Score = 132 bits (332), Expect = 2e-31
Identities = 90/205 (43%), Positives = 118/205 (56%), Gaps = 10/205 (4%)
Frame = +3
Query: 1 MDD---RENGRHKADQYKSAQGQWLMQ-QHQHQHPSM----KQIMSIMAERDAAIQERNL 52
MDD ENGRHK + Y+ A W QHQ + P+ K+I SIMAER AAI E L
Sbjct: 165 MDDGRQHENGRHKMEYYRGAHSLWNTDSQHQVKEPNALVMNKKIRSIMAERQAAILEIEL 344
Query: 53 --ALSEKKAALAERDMAFLQRDTAIAERNNALMERDNAIATLQFRENALANGGMSSCPPG 110
A+SEK ALA RD A QRD A+A+R+NAL+ERDNA+A LQ R N++ + P G
Sbjct: 345 EAAISEKNEALAARDAAIRQRDEALAQRDNALLERDNALAALQSRNNSV------NFPFG 506
Query: 111 CQISRGVKHIHHLPQQVNHLPNMGDSSYGTRELHTTDALPAAPVSLEVGKPPRRAKRPKE 170
I G K +HH NHL NM +++Y T+++ DA P + E ++KR KE
Sbjct: 507 GGIQCGSKRMHH---SSNHLSNMTEAAYNTKDMIIRDASPVTVIPSEAVN-SHQSKRSKE 674
Query: 171 SKSDSPNKKTPKSRKVKKEGDDLNK 195
+K + P KVKK G+DLN+
Sbjct: 675 NKVINSKASKPPC-KVKKMGEDLNR 746
>TC220483 similar to UP|Q9LDE2 (Q9LDE2) F10B6.5 (T5E21.17)
(At1g14680/F10B6_22) (Basic pentacysteine 2), partial
(29%)
Length = 524
Score = 125 bits (313), Expect = 4e-29
Identities = 50/82 (60%), Positives = 68/82 (81%)
Frame = +3
Query: 259 RQCYKWGNGGWQSACCTTTLSVYPLPAVPNKRHARVGGRKMSGSAFNKLLSRLAAEGHDL 318
+QCY+WG GGWQSACCTT +S+YPLP +R AR+ GRKMS AF K+L +LAAEG++
Sbjct: 6 QQCYRWGCGGWQSACCTTNVSIYPLPMSMKRRGARIAGRKMSQGAFKKVLEKLAAEGYNF 185
Query: 319 SHPVDLKDHWAKHGTNRYITIK 340
++P+DLK HWA+HGTN+++TI+
Sbjct: 186 ANPIDLKTHWARHGTNKFVTIR 251
>TC217887 GB|AAR25825.1|38639365|AY380572 basic pentacysteine 6 {Arabidopsis
thaliana;} , partial (13%)
Length = 567
Score = 90.5 bits (223), Expect = 1e-18
Identities = 42/43 (97%), Positives = 43/43 (99%)
Frame = +3
Query: 298 KMSGSAFNKLLSRLAAEGHDLSHPVDLKDHWAKHGTNRYITIK 340
KMSGSAFNKLLSRLAAEGHDLS+PVDLKDHWAKHGTNRYITIK
Sbjct: 15 KMSGSAFNKLLSRLAAEGHDLSNPVDLKDHWAKHGTNRYITIK 143
>AW099231 similar to GP|20530716|gb| GAGA-binding protein {Glycine max},
partial (26%)
Length = 259
Score = 80.1 bits (196), Expect = 1e-15
Identities = 35/72 (48%), Positives = 48/72 (66%)
Frame = +2
Query: 252 CSCTGVLRQCYKWGNGGWQSACCTTTLSVYPLPAVPNKRHARVGGRKMSGSAFNKLLSRL 311
C C+ +Q +KW +GGWQ+ACCTT + YPLP +R AR GR MS AF K +L
Sbjct: 5 CICSDATQQSHKWRSGGWQTACCTTGMPEYPLPMSTKRRGARTAGRIMSIGAFKKE*EKL 184
Query: 312 AAEGHDLSHPVD 323
AAEG++ S+P++
Sbjct: 185 AAEGYNFSNPIE 220
>BF426044 similar to SP|O49203|NDK3_ Nucleoside diphosphate kinase III
mitochondrial precursor (EC 2.7.4.6) (NDK III) (NDP
kinase III), partial (6%)
Length = 397
Score = 31.2 bits (69), Expect = 0.70
Identities = 17/50 (34%), Positives = 23/50 (46%)
Frame = +3
Query: 220 NKQLAISKADWKPQDLALNQVAYDDSTMPAPACSCTGVLRQCYKWGNGGW 269
NK + S AD D ++Q D+S MP+P+ VL W G W
Sbjct: 3 NKNRSESNADCTGHDSLIHQEGLDESPMPSPSRYGPAVLSH*LSW*PGWW 152
>TC214278 similar to UP|P93704 (P93704) HMG-1, partial (98%)
Length = 932
Score = 31.2 bits (69), Expect = 0.70
Identities = 17/40 (42%), Positives = 24/40 (59%)
Frame = +1
Query: 172 KSDSPNKKTPKSRKVKKEGDDLNKTMFANNKELEWKSSEE 211
KS S +K P +K+ + KT+ A NK+LE K+SEE
Sbjct: 517 KSLSDAEKAPFVATAEKKKQEYEKTISAYNKQLEGKNSEE 636
>TC216885
Length = 1122
Score = 30.4 bits (67), Expect = 1.2
Identities = 23/51 (45%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
Frame = -3
Query: 117 VKHIHHLPQ-QVNHLPNMGD-SSYGTRELHTTDALPAAPVSLEVGKPPRRA 165
+++IH LP H PN+G SS GTR+ H ALP P SL P+RA
Sbjct: 1051 LQNIHSLPM*DYEHAPNIGILSSIGTRQ-HGQTALPFFPYSL-----PQRA 917
>CF921606
Length = 518
Score = 30.4 bits (67), Expect = 1.2
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = +2
Query: 283 LPAVPNKRHARVGGRKMSGSAFNKLL 308
+P P K+H R GG+K G A N LL
Sbjct: 413 IPQGPPKKHPRAGGKKKGGGAGNPLL 490
>BM143757 GP|20530716|gb GAGA-binding protein {Glycine max}, partial (5%)
Length = 381
Score = 30.0 bits (66), Expect = 1.6
Identities = 9/14 (64%), Positives = 14/14 (99%)
Frame = +2
Query: 327 HWAKHGTNRYITIK 340
+WAKHGTN+++TI+
Sbjct: 5 YWAKHGTNKFVTIR 46
>TC217618 similar to GB|AAO64868.1|29028978|BT005933 At5g50860 {Arabidopsis
thaliana;} , partial (60%)
Length = 1325
Score = 30.0 bits (66), Expect = 1.6
Identities = 26/77 (33%), Positives = 30/77 (38%), Gaps = 13/77 (16%)
Frame = -1
Query: 181 PKSRKVKKEGDDLNKTMFANNKELEW---KSSEEIINGDDD----------LNKQLAISK 227
P SR K +G D + NNK EW S E IN DD L LA S
Sbjct: 1268 PHSRMPKSKGTDYLLELLLNNKSNEWPIADSLNERINFQDDAIWESALLVFLPLSLAAS* 1089
Query: 228 ADWKPQDLALNQVAYDD 244
PQD N V ++
Sbjct: 1088 LVTDPQDFESNMVEAEE 1038
>BE331076 weakly similar to GP|8131699|dbj cytoplasmic ribosomal protein S13
{Panax ginseng}, partial (71%)
Length = 355
Score = 29.6 bits (65), Expect = 2.0
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = +3
Query: 274 CTTTLSVYPLPAVPNKRHARVGGRKM 299
CTT + VYPL P K H G R +
Sbjct: 9 CTTAVKVYPLLLCPTKGHLLAGSRSL 86
>TC231428 UP|Q8S901 (Q8S901) Syringolide-induced protein 14-1-1, complete
Length = 1150
Score = 28.5 bits (62), Expect = 4.6
Identities = 40/180 (22%), Positives = 79/180 (43%), Gaps = 15/180 (8%)
Frame = +1
Query: 69 LQRDTAIAERNNALMERDNAIA--TLQFRENALANGGMSSCPPGCQISRGVKHIHHLPQQ 126
+Q +TA ++ L A+A T+ F+ + + G P V I H ++
Sbjct: 100 VQGETAYRMKSKILKALPKAVAAVTVTFQNHPFSPGRDHKSKPI------VSMIPHEARR 261
Query: 127 VNHLPNMGDSSYGTRELHTTDALPAAPVSLEVGKPPRRAKRPKESKSDSPN--------- 177
H + G++ G E+++ + P +P +G+ + K+ K+SKS S +
Sbjct: 262 KTH--DRGNN--GIDEIYSQE--PTSPKISCMGQIKHKKKQIKKSKSKSKSMTKEARNVA 423
Query: 178 -KKTPKSRK---VKKEGDDLNKTMFANNKELEWKSSEEIINGDDDLNKQLAISKADWKPQ 233
KKT + + VKK +K + + + + + + + D+NK LA++ A P+
Sbjct: 424 SKKTSSASRDIEVKKHVSTFHKMLLFHGAKPKSEGRKSNASAPGDINKDLALADAARAPR 603
>BI784937
Length = 428
Score = 28.1 bits (61), Expect = 6.0
Identities = 19/61 (31%), Positives = 23/61 (37%)
Frame = -2
Query: 124 PQQVNHLPNMGDSSYGTRELHTTDALPAAPVSLEVGKPPRRAKRPKESKSDSPNKKTPKS 183
P + P S T+ T A P L PPRR++RP S P TP
Sbjct: 322 PTVLRWAPRWRAVSRTTKRSSPTSA*ACRPTGLRSSPPPRRSRRPGSLPSSRPT-ATPGR 146
Query: 184 R 184
R
Sbjct: 145 R 143
>TC231082 weakly similar to UP|O83031 (O83031) Orf509e (Fragment), partial
(13%)
Length = 1383
Score = 28.1 bits (61), Expect = 6.0
Identities = 16/54 (29%), Positives = 24/54 (43%), Gaps = 7/54 (12%)
Frame = +1
Query: 161 PPRRAKRPKESKSDSPNKKTPKSRKVKKEG-------DDLNKTMFANNKELEWK 207
PP A P +K SP +K+P+S K G D ++ ++ L WK
Sbjct: 226 PPESAPSPNPTKKSSPQRKSPQSNY*KN*GFPIPSSEDSSVRSSAESSSTLTWK 387
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.314 0.128 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,418,972
Number of Sequences: 63676
Number of extensions: 220108
Number of successful extensions: 1419
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 1378
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1392
length of query: 340
length of database: 12,639,632
effective HSP length: 98
effective length of query: 242
effective length of database: 6,399,384
effective search space: 1548650928
effective search space used: 1548650928
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 59 (27.3 bits)
Medicago: description of AC124216.5