BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124215.9 - phase: 0
(475 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC215655 701 0.0
TC215653 650 0.0
CA801699 similar to GP|16226834|gb AT3g54190/F24B22_150 {Arabido... 251 4e-67
TC215652 166 2e-41
TC215654 129 3e-30
BM527806 homologue to GP|16226834|gb| AT3g54190/F24B22_150 {Arab... 59 5e-09
TC209514 weakly similar to UP|Q9BYV5 (Q9BYV5) Ring finger protei... 44 2e-04
TC225287 33 0.36
TC207414 31 1.1
TC204925 similar to GB|AAO39903.1|28372842|BT003675 At5g67590 {A... 30 1.8
CO985175 30 2.4
TC213906 similar to UP|Q9LT51 (Q9LT51) Xylulose kinase, partial ... 30 3.1
TC228838 similar to UP|Q9M3V1 (Q9M3V1) Protein phpsphatase 2C (P... 29 4.0
TC227325 UP|O04133 (O04133) SRC2, complete 29 5.2
TC228156 similar to UP|Q43638 (Q43638) Heat-shock protein precur... 28 6.8
TC210726 28 6.8
TC219105 similar to GB|AAM10098.1|20148415|AY081536 endomembrane... 28 8.9
BI470457 28 8.9
TC230269 weakly similar to UP|WSC2_YEAST (P53832) Cell wall inte... 28 8.9
TC229185 28 8.9
>TC215655
Length = 2122
Score = 701 bits (1808), Expect = 0.0
Identities = 360/473 (76%), Positives = 402/473 (84%), Gaps = 9/473 (1%)
Frame = +2
Query: 12 RRIVATKGESSNNGSKPNIKINNKNIIKKLQTREISPKPHRSFVAATSPHRFQNMRLTHQ 71
RR+VA K +P + N +KKLQ REIS R+F + RF+N+RL +
Sbjct: 299 RRVVAKK--------RPRLVDGFVNSVKKLQRREISSNRDRAFSMTDAQERFRNIRLQEE 454
Query: 72 FDTHDPKHHSSPSPFLPFLMKRTKVVEIVAAKNIVFALAHSGLCAAFSRETNERICFLNI 131
+DTHDPK SS L FL KR+K++EIVAA++IVFALA SG+CAAFSRETNERICFLN+
Sbjct: 455 YDTHDPKGPSSA--VLQFLRKRSKIIEIVAARDIVFALAQSGVCAAFSRETNERICFLNV 628
Query: 132 CPDEVIRSLFYNKNNDSLITVSVYASENFSSLKCRSTRIEYIKRAKPDAGFPLFQSESLK 191
PDEVIRSLFYNKNNDSLITVSVYAS+N+SSLKCRSTRIEYI+R KPDAGF LF+SESLK
Sbjct: 629 SPDEVIRSLFYNKNNDSLITVSVYASDNYSSLKCRSTRIEYIRRVKPDAGFALFESESLK 808
Query: 192 WPGFVEFDDVNAKVLTYSAQDSIYKVFDLKNYTLLYSISDRNVQEIKISPGIMLLIFNRA 251
WPGFVEFDDVN KVLTYSAQDSIYKVFDLKNY++LYSISD+NVQEIKISPGIMLLIF ++
Sbjct: 809 WPGFVEFDDVNGKVLTYSAQDSIYKVFDLKNYSMLYSISDKNVQEIKISPGIMLLIFTKS 988
Query: 252 SGHIPLKIISIEDGTVLKAFNHLLHRNKKVDFIEQFNEKLLVKQENENLQILDVRSSELM 311
S H+PLKI+SIEDGTVLK+FNHLLHRNKKVDFIEQFNEKLLVKQENENLQILDVR+SEL
Sbjct: 989 SSHVPLKILSIEDGTVLKSFNHLLHRNKKVDFIEQFNEKLLVKQENENLQILDVRTSELT 1168
Query: 312 EVSRTEFMTPSAFIFLYENQLFLTFRNRTVSVWNFRGELVTSFEDHLLWHPDCNTNNIYI 371
EVSR+EFMTPSAFIFLYENQLFLTFRNRTV+VWNFRGELVTSFEDHLLWHPDCNTNNIYI
Sbjct: 1169EVSRSEFMTPSAFIFLYENQLFLTFRNRTVAVWNFRGELVTSFEDHLLWHPDCNTNNIYI 1348
Query: 372 TSDQDLIISYCKAESEDQWMEANAGSINVSNILTGKCVAKINAAN-------CISKVDDC 424
TSDQDLIISYCKA+S+D E N GSINVSNILTGKC+AKI A+N C D+C
Sbjct: 1349TSDQDLIISYCKADSDDSLSEGN-GSINVSNILTGKCLAKIRASNGLPMVKECSCSDDNC 1525
Query: 425 SSTC--SCKHTDSSQLRSSVAEALEDITALFYDEDRNEIYTGNRHGFVHVWSN 475
SS+ S K SS++RS+VAEALEDITALFYDE+RNEIYTGNR G VHVWSN
Sbjct: 1526SSSACNSGKRKRSSRIRSTVAEALEDITALFYDEERNEIYTGNRLGLVHVWSN 1684
>TC215653
Length = 1524
Score = 650 bits (1678), Expect = 0.0
Identities = 328/412 (79%), Positives = 364/412 (87%), Gaps = 9/412 (2%)
Frame = +1
Query: 73 DTHDPKHHSSPSPFLPFLMKRTKVVEIVAAKNIVFALAHSGLCAAFSRETNERICFLNIC 132
DTHDPK SS L FL KR+K++EIVAA++IVFALA SG+CAAFSRE N+RICFLN+
Sbjct: 1 DTHDPKGPSSM--VLQFLRKRSKIIEIVAARDIVFALAQSGVCAAFSRENNQRICFLNVS 174
Query: 133 PDEVIRSLFYNKNNDSLITVSVYASENFSSLKCRSTRIEYIKRAKPDAGFPLFQSESLKW 192
PDEVIRSLFYNKNNDSLITVSVYAS+++SSLKCRSTRIEYI+R PDAGF LF+SESLKW
Sbjct: 175 PDEVIRSLFYNKNNDSLITVSVYASDSYSSLKCRSTRIEYIRRGTPDAGFALFESESLKW 354
Query: 193 PGFVEFDDVNAKVLTYSAQDSIYKVFDLKNYTLLYSISDRNVQEIKISPGIMLLIFNRAS 252
PGFVEFDDVN KVLTYSAQDSIYKVFDLKNYT+LYSISD+NVQEIKISPGIMLLI+ +AS
Sbjct: 355 PGFVEFDDVNGKVLTYSAQDSIYKVFDLKNYTMLYSISDKNVQEIKISPGIMLLIYAKAS 534
Query: 253 GHIPLKIISIEDGTVLKAFNHLLHRNKKVDFIEQFNEKLLVKQENENLQILDVRSSELME 312
H+PLKI+SIEDGT+LK+F+HLL+RNKKVDFIEQFNEKLLVKQENENLQILDVR+ E E
Sbjct: 535 SHVPLKILSIEDGTILKSFSHLLYRNKKVDFIEQFNEKLLVKQENENLQILDVRTFEHTE 714
Query: 313 VSRTEFMTPSAFIFLYENQLFLTFRNRTVSVWNFRGELVTSFEDHLLWHPDCNTNNIYIT 372
V R+EFMTPSAFIFLYENQLFLTFRNRTV+VWNFRGELVTSFEDHLLWHPDCNTNNIYIT
Sbjct: 715 VCRSEFMTPSAFIFLYENQLFLTFRNRTVAVWNFRGELVTSFEDHLLWHPDCNTNNIYIT 894
Query: 373 SDQDLIISYCKAESEDQWMEANAGSINVSNILTGKCVAKINAANCISKVDDCS------- 425
SDQDLIISYCKA+S+D E NAGSINVSNILTGKC+AKI A+N ++CS
Sbjct: 895 SDQDLIISYCKADSDDSLSEGNAGSINVSNILTGKCLAKIRASNSFPVDNNCSCSDKPSR 1074
Query: 426 STC--SCKHTDSSQLRSSVAEALEDITALFYDEDRNEIYTGNRHGFVHVWSN 475
S C S K S++RS+VAEALEDITALFYDE+RNEIYTGNR G VHVWSN
Sbjct: 1075SYCCNSKKRKHGSKMRSTVAEALEDITALFYDEERNEIYTGNRLGLVHVWSN 1230
>CA801699 similar to GP|16226834|gb AT3g54190/F24B22_150 {Arabidopsis
thaliana}, partial (29%)
Length = 438
Score = 251 bits (642), Expect = 4e-67
Identities = 122/146 (83%), Positives = 131/146 (89%), Gaps = 1/146 (0%)
Frame = +1
Query: 318 FMTPSAFIFLYENQLFLTFRNRTVSVWNFRGELVTSFEDHLLWHPDCNTNNIYITSDQDL 377
FMTPSAFIFLYENQLFL+FRNRTVSVWNFRGELVTSFEDH LWHPDCNTNNIYITSDQDL
Sbjct: 1 FMTPSAFIFLYENQLFLSFRNRTVSVWNFRGELVTSFEDHQLWHPDCNTNNIYITSDQDL 180
Query: 378 IISYCKAESEDQWMEANAGSINVSNILTGKCVAKINAANCISKVDDC-SSTCSCKHTDSS 436
IISYCKA+S+DQW+E AGSINVSNILTGKCVAKI A + +K D+C SS CSCK + S
Sbjct: 181 IISYCKADSDDQWIEGTAGSINVSNILTGKCVAKITAISSSAKADECRSSGCSCKKSHSC 360
Query: 437 QLRSSVAEALEDITALFYDEDRNEIY 462
+RS VAEALEDITALFYDEDRNEIY
Sbjct: 361 LMRSPVAEALEDITALFYDEDRNEIY 438
>TC215652
Length = 592
Score = 166 bits (420), Expect = 2e-41
Identities = 84/119 (70%), Positives = 93/119 (77%), Gaps = 7/119 (5%)
Frame = +2
Query: 364 CNTNNIYITSDQDLIISYCKAESEDQWMEANAGSINVSNILTGKCVAKINAANCISKVDD 423
CNTNNIYITSDQDLIISYCKA+S+D E NAGSINVSNILTGKC+AKI A+N +
Sbjct: 2 CNTNNIYITSDQDLIISYCKADSDDSXSEGNAGSINVSNILTGKCLAKIRASNSFPVDNS 181
Query: 424 C----SSTCSC---KHTDSSQLRSSVAEALEDITALFYDEDRNEIYTGNRHGFVHVWSN 475
C S +C C K S+ RS+VAEALEDITALFYDE+RNEIYTGNRHG VHVWSN
Sbjct: 182 CSDNPSGSCCCNSKKRKHGSKTRSTVAEALEDITALFYDEERNEIYTGNRHGLVHVWSN 358
>TC215654
Length = 623
Score = 129 bits (324), Expect = 3e-30
Identities = 69/104 (66%), Positives = 79/104 (75%), Gaps = 9/104 (8%)
Frame = +3
Query: 381 YCKAESEDQWMEANAGSINVSNILTGKCVAKINAAN-------CISKVDDCSSTC--SCK 431
YCKA+S+D E N GSINVSNILTGKC+AKI A+N C D+CSS+ S K
Sbjct: 3 YCKADSDDSLSEGN-GSINVSNILTGKCLAKIRASNGFPMVKECSCSDDNCSSSACNSGK 179
Query: 432 HTDSSQLRSSVAEALEDITALFYDEDRNEIYTGNRHGFVHVWSN 475
SS++RS+VAEALEDITALFYDE+RNEIYTGNRHG VHVWSN
Sbjct: 180 QKHSSRIRSTVAEALEDITALFYDEERNEIYTGNRHGLVHVWSN 311
>BM527806 homologue to GP|16226834|gb| AT3g54190/F24B22_150 {Arabidopsis
thaliana}, partial (7%)
Length = 196
Score = 58.9 bits (141), Expect = 5e-09
Identities = 35/65 (53%), Positives = 45/65 (68%), Gaps = 6/65 (9%)
Frame = +2
Query: 96 VVEIVAAKNIVFALAHSGLCAAFSRET--NERICFLNICPDEVIR----SLFYNKNNDSL 149
++EIVAA++IVFALA SG+CAAFSRET ++ L C ++ +F KNNDSL
Sbjct: 2 IIEIVAARDIVFALAQSGVCAAFSRETXMXKKFMLLXKCSSP*MKL*GEFVFIIKNNDSL 181
Query: 150 ITVSV 154
ITVSV
Sbjct: 182 ITVSV 196
>TC209514 weakly similar to UP|Q9BYV5 (Q9BYV5) Ring finger protein 29,
partial (4%)
Length = 434
Score = 43.9 bits (102), Expect = 2e-04
Identities = 25/62 (40%), Positives = 34/62 (54%)
Frame = +2
Query: 12 RRIVATKGESSNNGSKPNIKINNKNIIKKLQTREISPKPHRSFVAATSPHRFQNMRLTHQ 71
RRIVA K +P N +KKLQ REIS K R+F + + RF+N+RL +
Sbjct: 269 RRIVAAK-------KRPRANDGFVNSVKKLQRREISSKRDRAFTMSDAQERFRNIRLQEE 427
Query: 72 FD 73
+D
Sbjct: 428 YD 433
>TC225287
Length = 1825
Score = 32.7 bits (73), Expect = 0.36
Identities = 16/46 (34%), Positives = 24/46 (51%)
Frame = -2
Query: 117 AFSRETNERICFLNICPDEVIRSLFYNKNNDSLITVSVYASENFSS 162
A SRE + IC + CP ++ LFY +N + V +Y + SS
Sbjct: 255 ATSREIHGSICSPHSCPLNLVNFLFYRASNGRIPNVGIYFYQKVSS 118
>TC207414
Length = 1165
Score = 31.2 bits (69), Expect = 1.1
Identities = 16/54 (29%), Positives = 27/54 (49%)
Frame = -2
Query: 131 ICPDEVIRSLFYNKNNDSLITVSVYASENFSSLKCRSTRIEYIKRAKPDAGFPL 184
+C D + SLF+ NND L S + S ++ L+C+ I ++ K P+
Sbjct: 723 VCHDRHVNSLFHQLNNDVLTQSSAHVS--YTLLQCQQLLIRHVVDIKAHFSVPV 568
>TC204925 similar to GB|AAO39903.1|28372842|BT003675 At5g67590 {Arabidopsis
thaliana;} , partial (56%)
Length = 500
Score = 30.4 bits (67), Expect = 1.8
Identities = 13/32 (40%), Positives = 19/32 (58%)
Frame = -2
Query: 62 RFQNMRLTHQFDTHDPKHHSSPSPFLPFLMKR 93
R+Q R HQ ++ P+ SSPS P L++R
Sbjct: 256 RYQKSRQNHQHESRGPQRMSSPSVDFPTLLRR 161
>CO985175
Length = 855
Score = 30.0 bits (66), Expect = 2.4
Identities = 28/99 (28%), Positives = 42/99 (42%), Gaps = 1/99 (1%)
Frame = -3
Query: 203 AKVLTYSAQDSIYKVFDLKN-YTLLYSISDRNVQEIKISPGIMLLIFNRASGHIPLKIIS 261
+ +L SA I +L N I RNV E+ I G + + +A HIP +
Sbjct: 712 SNLLGSSALKEIMNFVNLSNKIPFAKPIPFRNVSEVTIDSGTSMCLSKQAKTHIPRWLPD 533
Query: 262 IEDGTVLKAFNHLLHRNKKVDFIEQFNEKLLVKQENENL 300
+ N +L + +K D E+ E LL +EN L
Sbjct: 532 FPK----ENCNRVLVKERKCD--EKLWEHLLAAEENSGL 434
>TC213906 similar to UP|Q9LT51 (Q9LT51) Xylulose kinase, partial (27%)
Length = 599
Score = 29.6 bits (65), Expect = 3.1
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +3
Query: 115 CAAFSRETNERICFLNIC 132
C+ F RET+E IC L+IC
Sbjct: 345 CSIFCRETSEAICMLSIC 398
>TC228838 similar to UP|Q9M3V1 (Q9M3V1) Protein phpsphatase 2C (PP2C) ,
partial (40%)
Length = 873
Score = 29.3 bits (64), Expect = 4.0
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -3
Query: 78 KHHSSPSPFLPFLMKRTKVVEIVAAKNI 105
KHH++P F PFL+ +TK I + +
Sbjct: 610 KHHATPCIFFPFLLSKTKFHNIGCPRTV 527
>TC227325 UP|O04133 (O04133) SRC2, complete
Length = 1235
Score = 28.9 bits (63), Expect = 5.2
Identities = 21/73 (28%), Positives = 34/73 (45%), Gaps = 7/73 (9%)
Frame = -1
Query: 21 SSNNGSKPNIKI----NNKNIIKKLQTREISPKPHRSFVAATSPHRFQNMRLTHQFDTHD 76
++ G K N +I N +I+KL+ + + H+S SPH Q ++ T
Sbjct: 1061 ATEKGKK*NPEI*KEKKNTGLIEKLKIKNQIHQHHQS--QHHSPHHIQQHQIPFLRSTFH 888
Query: 77 PKHHSS---PSPF 86
P+HH + P PF
Sbjct: 887 PRHHQAILLPIPF 849
>TC228156 similar to UP|Q43638 (Q43638) Heat-shock protein precursor, partial
(54%)
Length = 1688
Score = 28.5 bits (62), Expect = 6.8
Identities = 11/29 (37%), Positives = 16/29 (54%)
Frame = -2
Query: 58 TSPHRFQNMRLTHQFDTHDPKHHSSPSPF 86
++PH FQ +TH+ H P H P+ F
Sbjct: 1054 SAPHEFQTGCITHRLCLHQPFHVCRPTKF 968
>TC210726
Length = 940
Score = 28.5 bits (62), Expect = 6.8
Identities = 13/33 (39%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
Frame = -3
Query: 51 HRSFVAATSPHR-FQNMRLTHQFDTHDPKHHSS 82
H SF++ + H+ + + HQ HDP HHSS
Sbjct: 332 HASFLSHSLVHQNYHDTEQNHQPYPHDPSHHSS 234
>TC219105 similar to GB|AAM10098.1|20148415|AY081536 endomembrane protein EMP70
precusor isolog {Arabidopsis thaliana;} , partial (59%)
Length = 1358
Score = 28.1 bits (61), Expect = 8.9
Identities = 16/33 (48%), Positives = 22/33 (66%), Gaps = 2/33 (6%)
Frame = -1
Query: 17 TKGESSNNGSKPNIK--INNKNIIKKLQTREIS 47
T ESS+NGS PNI+ N++ KK Q R++S
Sbjct: 1118 THNESSDNGSSPNIQHPNNSQQDDKKNQQRKLS 1020
>BI470457
Length = 419
Score = 28.1 bits (61), Expect = 8.9
Identities = 23/89 (25%), Positives = 39/89 (42%)
Frame = +2
Query: 33 NNKNIIKKLQTREISPKPHRSFVAATSPHRFQNMRLTHQFDTHDPKHHSSPSPFLPFLMK 92
+ K+ I +++ ISP S +++ S R+ + H F TH S F+ FLM+
Sbjct: 95 SGKSTITHVKSSRISPT--HSLLSSISTDRYHYQQQLHPFSTHASSFSFSHRAFV-FLMQ 265
Query: 93 RTKVVEIVAAKNIVFALAHSGLCAAFSRE 121
+ + HS C++ SRE
Sbjct: 266 TLIATKWT*IWEVQAR*QHSIACSSSSRE 352
>TC230269 weakly similar to UP|WSC2_YEAST (P53832) Cell wall integrity and
stress response component 2 precursor, partial (6%)
Length = 696
Score = 28.1 bits (61), Expect = 8.9
Identities = 17/46 (36%), Positives = 21/46 (44%), Gaps = 1/46 (2%)
Frame = -2
Query: 45 EISPKPHRSFVAATSPHRFQNMRLTHQFDTHDPKH-HSSPSPFLPF 89
+ SP P R + P F + L+ Q H SSPSPFL F
Sbjct: 206 QFSPHPKRPPRCSPYPSSFSSSSLSSQLQPPQSLHLPSSPSPFLAF 69
>TC229185
Length = 673
Score = 28.1 bits (61), Expect = 8.9
Identities = 20/74 (27%), Positives = 30/74 (40%)
Frame = +1
Query: 182 FPLFQSESLKWPGFVEFDDVNAKVLTYSAQDSIYKVFDLKNYTLLYSISDRNVQEIKISP 241
FP +E F F D+ KV+ +S+ + F K DR + +K P
Sbjct: 67 FPRHSNEETYMKVFSSFKDI--KVIQFSSMQDAFAGFSDKER------EDRFRKRVKRYP 222
Query: 242 GIMLLIFNRASGHI 255
GI + + GHI
Sbjct: 223 GIWCCVLDHTPGHI 264
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.134 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,655,128
Number of Sequences: 63676
Number of extensions: 307476
Number of successful extensions: 1858
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 1839
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1849
length of query: 475
length of database: 12,639,632
effective HSP length: 101
effective length of query: 374
effective length of database: 6,208,356
effective search space: 2321925144
effective search space used: 2321925144
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC124215.9