BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC123975.11 - phase: 0
(206 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC226656 similar to UP|O81098 (O81098) RNA polymerase I, II and ... 370 e-103
TC226657 similar to UP|O81098 (O81098) RNA polymerase I, II and ... 367 e-102
AW704818 similar to PIR|T51950|T519 DNA-directed RNA polymerase ... 146 6e-36
TC209427 similar to GB|AAL84932.1|19310385|AY078926 At2g41340/F1... 136 8e-33
AW831252 similar to GP|21741703|emb oj991113_30.8 {Oryza sativa ... 92 1e-19
CO978999 85 2e-17
TC228139 similar to UP|Q8GVE1 (Q8GVE1) Chitin-inducible gibberel... 29 1.3
TC233357 homologue to UP|Q8VWW2 (Q8VWW2) Two-component response ... 27 6.5
TC229884 weakly similar to UP|Q94AX9 (Q94AX9) At1g72650/F28P22_1... 27 6.5
BE802984 27 8.5
TC232989 similar to UP|Q6YZX7 (Q6YZX7) Auxin efflux carrier prot... 27 8.5
>TC226656 similar to UP|O81098 (O81098) RNA polymerase I, II and III 24.3 kDa
subunit (AT3g22320/MCB17_5) , complete
Length = 876
Score = 370 bits (951), Expect = e-103
Identities = 183/206 (88%), Positives = 195/206 (93%)
Frame = +1
Query: 1 MVFSEEEITRLYRIRKTVMQMLKDRNYLVGDFELNMSKHDFKDKYGENMKREDLVINKTK 60
MV SEEEI RLYRIRKTVMQML+DRN+LVGDFE+NMSKH+FK KYGE+MKREDLVINK+K
Sbjct: 61 MVLSEEEIGRLYRIRKTVMQMLRDRNFLVGDFEINMSKHEFKSKYGEHMKREDLVINKSK 240
Query: 61 KDKPSDQIYVFFPEEAKVGVKTMKTYTNRMNSENVYRAILVCQTSLTPFAKTCVSEIASK 120
KD DQIYVFFP+E KVGVKTMKTYTNRMNSENVYRAILV QT+LTPFA+TC+SEI+SK
Sbjct: 241 KDNSGDQIYVFFPDEPKVGVKTMKTYTNRMNSENVYRAILVTQTNLTPFARTCISEISSK 420
Query: 121 FHLEVFQEAELLVNIKEHVLVPEHQILNDTEKKTLLERYTVKETQLPRIQVTDPVARYYG 180
FHLEVFQEAELLVNIKEH LVPEHQ+L D EKKTLLERYTVKETQLPRIQVTDPVARYYG
Sbjct: 421 FHLEVFQEAELLVNIKEHELVPEHQVLTDAEKKTLLERYTVKETQLPRIQVTDPVARYYG 600
Query: 181 LKRGQVVKIIRPSETAGRYVTYRFVV 206
LKRGQVVKIIRPSETAGRYVTYRFVV
Sbjct: 601 LKRGQVVKIIRPSETAGRYVTYRFVV 678
>TC226657 similar to UP|O81098 (O81098) RNA polymerase I, II and III 24.3 kDa
subunit (AT3g22320/MCB17_5) , complete
Length = 773
Score = 367 bits (942), Expect = e-102
Identities = 181/206 (87%), Positives = 194/206 (93%)
Frame = +3
Query: 1 MVFSEEEITRLYRIRKTVMQMLKDRNYLVGDFELNMSKHDFKDKYGENMKREDLVINKTK 60
MV SEEE+ RLYRIRKTVMQML+DR +LVGDFE+NMSKH+FK KYGE+MKREDLVINK+K
Sbjct: 36 MVLSEEEMGRLYRIRKTVMQMLRDRGFLVGDFEINMSKHEFKSKYGEHMKREDLVINKSK 215
Query: 61 KDKPSDQIYVFFPEEAKVGVKTMKTYTNRMNSENVYRAILVCQTSLTPFAKTCVSEIASK 120
KD DQIYVFFP+E KVGVKTMKTYTNRMNSENVYRAILV QT+LTPFA+TC+SEI+SK
Sbjct: 216 KDNSGDQIYVFFPDEPKVGVKTMKTYTNRMNSENVYRAILVTQTNLTPFARTCISEISSK 395
Query: 121 FHLEVFQEAELLVNIKEHVLVPEHQILNDTEKKTLLERYTVKETQLPRIQVTDPVARYYG 180
FHLEVFQEAELLVNIKEH LVPEHQ+L D EKKTLLERYTVKETQLPRIQVTDPVARYYG
Sbjct: 396 FHLEVFQEAELLVNIKEHELVPEHQVLTDAEKKTLLERYTVKETQLPRIQVTDPVARYYG 575
Query: 181 LKRGQVVKIIRPSETAGRYVTYRFVV 206
LKRGQVVKIIRPSETAGRYVTYRFVV
Sbjct: 576 LKRGQVVKIIRPSETAGRYVTYRFVV 653
>AW704818 similar to PIR|T51950|T519 DNA-directed RNA polymerase (EC 2.7.7.6)
23K chain [imported] - Arabidopsis thaliana, partial
(30%)
Length = 346
Score = 146 bits (369), Expect = 6e-36
Identities = 76/115 (66%), Positives = 91/115 (79%)
Frame = +2
Query: 13 RIRKTVMQMLKDRNYLVGDFELNMSKHDFKDKYGENMKREDLVINKTKKDKPSDQIYVFF 72
RI TVMQML R +LV DF +NMS + + +++K EDLVINK+KKD DQIYVFF
Sbjct: 2 RISYTVMQMLLYRGFLVVDF*INMSNS*VQVQVRQHIKLEDLVINKSKKDNSGDQIYVFF 181
Query: 73 PEEAKVGVKTMKTYTNRMNSENVYRAILVCQTSLTPFAKTCVSEIASKFHLEVFQ 127
P++ KV VKTMKTYTN MNS NVYRAILV QT+LTPFA+TC+ EI+SKFHL+VFQ
Sbjct: 182 PDDPKVCVKTMKTYTNLMNS*NVYRAILVTQTNLTPFARTCIIEISSKFHLDVFQ 346
>TC209427 similar to GB|AAL84932.1|19310385|AY078926 At2g41340/F13H10.11
{Arabidopsis thaliana;} , partial (50%)
Length = 699
Score = 136 bits (342), Expect = 8e-33
Identities = 76/200 (38%), Positives = 117/200 (58%), Gaps = 1/200 (0%)
Frame = +2
Query: 7 EITRLYRIRKTVMQMLKDRNYLVGDFELNMSKHDFKDKYGENMKREDLVINKTKKDKPSD 66
E R Y R+T ++MLKDR Y + E+++S DF+D +G+ + L ++ T S
Sbjct: 56 ESHRYYLSRRTTLEMLKDRGYSIPSHEIDLSLSDFRDMHGQFPDPDRLRLSLTHATNSSK 235
Query: 67 QIYVFFPEEAKVGVKTMKTYTNRM-NSENVYRAILVCQTSLTPFAKTCVSEIASKFHLEV 125
+I V F V V ++ ++ N + + IL+ + +T A V+ + F +E+
Sbjct: 236 RILVIFCGPGVVKVTAIRNIAGQIINRDTLTGLILIVENHITSQALKAVNLFS--FKVEI 409
Query: 126 FQEAELLVNIKEHVLVPEHQILNDTEKKTLLERYTVKETQLPRIQVTDPVARYYGLKRGQ 185
FQ +LLVNI +HVL P+HQ+L D +K LL++Y ++E QLPR+ TD +ARYYGL+RGQ
Sbjct: 410 FQITDLLVNITKHVLKPKHQVLTDKQKINLLKKYNIEEKQLPRMLQTDAIARYYGLERGQ 589
Query: 186 VVKIIRPSETAGRYVTYRFV 205
V K+ E +VTYR V
Sbjct: 590 VAKVTYSGEVTRMHVTYRCV 649
>AW831252 similar to GP|21741703|emb oj991113_30.8 {Oryza sativa (japonica
cultivar-group)}, partial (12%)
Length = 214
Score = 92.4 bits (228), Expect = 1e-19
Identities = 41/70 (58%), Positives = 56/70 (79%)
Frame = +2
Query: 4 SEEEITRLYRIRKTVMQMLKDRNYLVGDFELNMSKHDFKDKYGENMKREDLVINKTKKDK 63
SEEE+ RLYRIRKTVMQML+D+ +LVGDF++N+SKH F Y ++ ++L+INK+ KD
Sbjct: 5 SEEEMGRLYRIRKTVMQMLRDKGFLVGDFKINISKHYFNSNYYNHINHKNLIINKSNKDN 184
Query: 64 PSDQIYVFFP 73
+QIY+FFP
Sbjct: 185SINQIYIFFP 214
>CO978999
Length = 841
Score = 85.1 bits (209), Expect = 2e-17
Identities = 41/77 (53%), Positives = 56/77 (72%)
Frame = -1
Query: 130 ELLVNIKEHVLVPEHQILNDTEKKTLLERYTVKETQLPRIQVTDPVARYYGLKRGQVVKI 189
+LLVNI +HVL P+ Q+L D +KK LL++Y +KE QLPR+ +TD +ARYYGL+RGQVVK+
Sbjct: 334 DLLVNITKHVLKPKQQVLTDKQKKNLLKKYNIKEKQLPRMLLTDAIARYYGLERGQVVKV 155
Query: 190 IRPSETAGRYVTYRFVV 206
E +V F+V
Sbjct: 154 TYSGEITQMHVW**FLV 104
>TC228139 similar to UP|Q8GVE1 (Q8GVE1) Chitin-inducible
gibberellin-responsive protein, partial (47%)
Length = 1497
Score = 29.3 bits (64), Expect = 1.3
Identities = 15/58 (25%), Positives = 32/58 (54%), Gaps = 3/58 (5%)
Frame = +1
Query: 136 KEHVLVPEHQILNDTEKKTLLERYTVKETQLPRIQVT---DPVARYYGLKRGQVVKII 190
++H+ + + QI T+ TLL+ + P +++T DPV++Y RG ++++
Sbjct: 61 EDHIHIIDFQIAQGTQWMTLLQALAARPGGAPHVRITGIDDPVSKY---ARGDGLEVV 225
>TC233357 homologue to UP|Q8VWW2 (Q8VWW2) Two-component response
regulator-like protein, partial (8%)
Length = 811
Score = 26.9 bits (58), Expect = 6.5
Identities = 15/44 (34%), Positives = 27/44 (61%)
Frame = +1
Query: 99 ILVCQTSLTPFAKTCVSEIASKFHLEVFQEAELLVNIKEHVLVP 142
IL T+++P + TC + +++ H E+ QE L+ +I V+VP
Sbjct: 223 ILNVVTAMSPLS-TCGTARSTRSHFEILQEITLVSSIVLLVIVP 351
>TC229884 weakly similar to UP|Q94AX9 (Q94AX9) At1g72650/F28P22_16 (MYB
transcription factor), partial (20%)
Length = 1244
Score = 26.9 bits (58), Expect = 6.5
Identities = 10/18 (55%), Positives = 13/18 (71%)
Frame = -1
Query: 25 RNYLVGDFELNMSKHDFK 42
RN+L G F LN+ HD+K
Sbjct: 614 RNFLAGSFRLNLPIHDWK 561
>BE802984
Length = 421
Score = 26.6 bits (57), Expect = 8.5
Identities = 12/36 (33%), Positives = 18/36 (49%)
Frame = +1
Query: 137 EHVLVPEHQILNDTEKKTLLERYTVKETQLPRIQVT 172
+H +P +L KK +L R+T LPR+ T
Sbjct: 232 QHHSIPFMALLTSQPKKPILHRFTTLHRPLPRLDRT 339
>TC232989 similar to UP|Q6YZX7 (Q6YZX7) Auxin efflux carrier protein-like,
partial (31%)
Length = 497
Score = 26.6 bits (57), Expect = 8.5
Identities = 16/70 (22%), Positives = 32/70 (44%), Gaps = 2/70 (2%)
Frame = +1
Query: 91 NSENVYRAIL--VCQTSLTPFAKTCVSEIASKFHLEVFQEAELLVNIKEHVLVPEHQILN 148
N+ N+ A++ VC T PF K C + + L + L+ + H++ P +
Sbjct: 64 NTGNLLLAVVGSVCHTKDNPFGKNCNTRGVAYVSLSQWVSVILVYTLVYHMMEPPIEYYE 243
Query: 149 DTEKKTLLER 158
E++ +E+
Sbjct: 244 IVEEEAEIEQ 273
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.135 0.376
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,687,856
Number of Sequences: 63676
Number of extensions: 75759
Number of successful extensions: 351
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 350
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 350
length of query: 206
length of database: 12,639,632
effective HSP length: 93
effective length of query: 113
effective length of database: 6,717,764
effective search space: 759107332
effective search space used: 759107332
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC123975.11