BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122730.8 + phase: 0 /pseudo
(739 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC220322 82 2e-30
TC210254 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F1... 89 7e-18
TC222000 weakly similar to PIR|H84710|H84710 Mutator-like transp... 88 1e-17
BM308209 52 9e-14
TC220753 57 4e-08
AI856054 54 3e-07
TC223710 similar to UP|Q8LDF8 (Q8LDF8) RING zinc finger protein-... 41 0.002
TC215140 similar to GB|AAL32703.1|17065098|AY062625 nucleotide s... 40 0.003
TC207334 similar to UP|Q949K3 (Q949K3) Suppressor-like protein, ... 40 0.003
TC214000 40 0.003
TC234897 weakly similar to UP|TIR3_YEAST (P40552) Cell wall prot... 39 0.006
BE806360 similar to GP|6175165|gb| Mutator-like transposase {Ara... 39 0.011
TC215592 similar to UP|Q9SSS9 (Q9SSS9) H+-transporting ATP synth... 38 0.014
TC223428 similar to UP|Q8VZE1 (Q8VZE1) At2g17250/T23A1.11, parti... 38 0.014
BM892538 similar to GP|9759296|dbj| gamma-tubulin interacting pr... 38 0.019
TC228693 similar to UP|Q8S9A6 (Q8S9A6) Glucosyltransferase-3, pa... 38 0.019
TC218974 weakly similar to UP|PCD7_MOUSE (Q9WTY1) Programmed cel... 38 0.019
BF595364 37 0.024
TC225689 homologue to UP|RSP4_SOYBN (O22518) 40S ribosomal prote... 37 0.032
BG043254 37 0.032
>TC220322
Length = 1264
Score = 81.6 bits (200), Expect(2) = 2e-30
Identities = 59/201 (29%), Positives = 90/201 (44%)
Frame = +2
Query: 377 RAAKATYTTAFDREMNEIKKISEEAYNHLLQTPVKHWSKSKFSNGPLCDTLVNNMTEAFN 436
+ AK+T F+ M +K I+ +A+ +L + P + W+K+ FS P D + NN E FN
Sbjct: 2 QCAKSTTVAEFEGHMAHLKTINCQAWEYLNKWPKQAWTKAHFSTIPKVDNICNNTCEVFN 181
Query: 437 SVIVVLRKKPIVTMLEDIRVYLMERWEKNRQKVANYADNILPNIRKKI*KRV*FYKQLGG 496
I+ R KPI+TMLE+IR Y+M + K++ + K++ K F Q
Sbjct: 182 FRILQYRCKPIITMLEEIRSYIMRTMAARKVKLSGKPGPLCLVQYKRLEKEFHFANQWTP 361
Query: 497 QVCRQQL*TLN*Y*IYFLFVLYPLV*HLYAWNVLVRRAIEYDYEIRHRSMNGDSYSINLL 556
C N+ +R YE+ M G+ +NL
Sbjct: 362 IWCGD--------------------------NMGLR------YEVH---MWGNKVEVNLA 436
Query: 557 RKECSCRKWLLTGLPCCHAIS 577
C+C W LTG+PC HAI+
Sbjct: 437 GWTCTCGVWQLTGMPCRHAIA 499
Score = 69.7 bits (169), Expect(2) = 2e-30
Identities = 37/95 (38%), Positives = 47/95 (48%), Gaps = 1/95 (1%)
Frame = +1
Query: 597 ERYEICYSHIIYPVNGQSLWRRTEYTDLQPPPIKRQPGRPKKNRRKDVDEKR-DEQQLKK 655
E Y Y H I PV G W +T+YT PP + Q GRPKKNR++ VDE +LK+
Sbjct: 559 EAYNKTYQHFIEPVQGPQYWAQTQYTHPVPPYKRVQRGRPKKNRKRSVDEDNVTGHKLKR 738
Query: 656 AKYGMKCSRCKAEGHNKSTCKLPPPPPQPEPGTQP 690
C RC HN +CK P +P+ P
Sbjct: 739 KLAEFTCGRCGQTNHNIRSCKNIGVPVRPKKYVAP 843
>TC210254 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17A9_9
{Arabidopsis thaliana;} , partial (6%)
Length = 814
Score = 89.0 bits (219), Expect = 7e-18
Identities = 51/147 (34%), Positives = 75/147 (50%), Gaps = 8/147 (5%)
Frame = +1
Query: 539 YEIRHRSMNGDSYSINLLRKECSCRKWLLTGLPCCHAISCMKSQDIDVDQYVSDCFRKER 598
+E+R S++ +++ +CSC+ W LTG+PCCHAI+ + Y S F E
Sbjct: 37 FEVRGESVD----IVDIDNWDCSCKGWQLTGVPCCHAIAVFECVGRSPYDYCSRYFTVEN 204
Query: 599 YEICYSHIIY--------PVNGQSLWRRTEYTDLQPPPIKRQPGRPKKNRRKDVDEKRDE 650
Y + Y+ I+ PV G+S T + PPP KR PGRPK + + +D
Sbjct: 205 YRLTYAESIHPVPNVDKPPVQGES----TALVMVIPPPTKRPPGRPKMKQVESID----- 357
Query: 651 QQLKKAKYGMKCSRCKAEGHNKSTCKL 677
K ++CS+CK GHN+ TCKL
Sbjct: 358 ----IIKRQLQCSKCKGLGHNRKTCKL 426
>TC222000 weakly similar to PIR|H84710|H84710 Mutator-like transposase
[imported] - Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (12%)
Length = 617
Score = 88.2 bits (217), Expect = 1e-17
Identities = 46/131 (35%), Positives = 68/131 (51%), Gaps = 7/131 (5%)
Frame = +2
Query: 553 INLLRKECSCRKWLLTGLPCCHAISCMKSQDIDVDQYVSDCFRKERYEICYSHIIYPVNG 612
+N+ CSCR W L G+PC HA + + S DV + CF + Y+ I+P+ G
Sbjct: 20 VNIGSHSCSCRDWQLNGIPCSHAAAALISCRKDVYAFSQKCFTAASFRDTYAETIHPIPG 199
Query: 613 QSLWRRTEYTDL-------QPPPIKRQPGRPKKNRRKDVDEKRDEQQLKKAKYGMKCSRC 665
+ W +T + + +PP ++R PGRP+K R VD+ L + K+ + CSRC
Sbjct: 200 KLEWSKTGNSSMDDNILVVRPPKLRRPPGRPEK--RMCVDD------LNREKHTVHCSRC 355
Query: 666 KAEGHNKSTCK 676
GH K TCK
Sbjct: 356 NQTGHYKRTCK 388
>BM308209
Length = 435
Score = 51.6 bits (122), Expect(2) = 9e-14
Identities = 24/56 (42%), Positives = 35/56 (61%)
Frame = +2
Query: 390 EMNEIKKISEEAYNHLLQTPVKHWSKSKFSNGPLCDTLVNNMTEAFNSVIVVLRKK 445
E IK+++ EAY+ L++ K W K FS CD L+NN+ +AFNS I+ R+K
Sbjct: 110 EDEHIKEVNNEAYDWLIKVGTKLWRKHTFSYYTKCDVLMNNLCDAFNSTILCAREK 277
Score = 43.9 bits (102), Expect(2) = 9e-14
Identities = 21/42 (50%), Positives = 30/42 (71%), Gaps = 1/42 (2%)
Frame = +3
Query: 356 RHLYNNFRKKY-PGKKLKELMWRAAKATYTTAFDREMNEIKK 396
RH+YNNF+ K+ G L+ LM RAAKA++ A+D +MN K+
Sbjct: 6 RHIYNNFKNKFVGGTLLRNLMMRAAKASFEEAWDEKMNISKR 131
Score = 32.0 bits (71), Expect = 1.0
Identities = 15/41 (36%), Positives = 23/41 (55%)
Frame = +3
Query: 539 YEIRHRSMNGDSYSINLLRKECSCRKWLLTGLPCCHAISCM 579
+E+ H SM D + +NL CS W +TG+ C HA + +
Sbjct: 315 FEVSH-SMLSDKFIVNLNSWSCS*NLWEITGILCRHACAAI 434
>TC220753
Length = 1312
Score = 56.6 bits (135), Expect = 4e-08
Identities = 36/130 (27%), Positives = 57/130 (43%), Gaps = 2/130 (1%)
Frame = +2
Query: 548 GDSYSINLLRKECSCRKWLLTGLPCCHAISCMKSQDIDVDQYVSDCFRKERYEICYSHII 607
G + ++ L C ++ LPC HAI+ +++ D +V ++ E Y H
Sbjct: 200 GMACTVKLNEWLCDYGQFQALRLPCSHAIAACAFCNLNYDDFVDPVYKLENIFKVYQHHF 379
Query: 608 YPVNGQSLWRRTEYTDLQPPPIKRQ--PGRPKKNRRKDVDEKRDEQQLKKAKYGMKCSRC 665
+ + + W + P KR+ GRP R + + DE + K KCS C
Sbjct: 380 HSLGSEDTWPQYLGPHFMSDPSKRRQISGRPATTR---IHNEMDESIPNRLK---KCSLC 541
Query: 666 KAEGHNKSTC 675
K EGHN+S C
Sbjct: 542 KTEGHNQSNC 571
>AI856054
Length = 173
Score = 53.5 bits (127), Expect = 3e-07
Identities = 25/45 (55%), Positives = 34/45 (75%)
Frame = -1
Query: 433 EAFNSVIVVLRKKPIVTMLEDIRVYLMERWEKNRQKVANYADNIL 477
E+FNSVI+ R+K IV+MLEDI +YLMERW+ N +KV ++L
Sbjct: 137 ESFNSVILEAREKLIVSMLEDIHIYLMERWDTNSKKVVAREGSVL 3
>TC223710 similar to UP|Q8LDF8 (Q8LDF8) RING zinc finger protein-like,
partial (49%)
Length = 465
Score = 40.8 bits (94), Expect = 0.002
Identities = 20/45 (44%), Positives = 24/45 (52%)
Frame = -3
Query: 678 PPPPPQPEPGTQPPATQFPTSQPTSDHAPTTQVSATQASASQVST 722
PPPPP P P T PPA FP+S P + +P Q + S ST
Sbjct: 253 PPPPPSPSPRTSPPA-GFPSSAPPTPPSPAPQCRIRRNRGSPCST 122
>TC215140 similar to GB|AAL32703.1|17065098|AY062625 nucleotide sugar
epimerase-like protein {Arabidopsis thaliana;} , partial
(91%)
Length = 1885
Score = 40.4 bits (93), Expect = 0.003
Identities = 23/61 (37%), Positives = 30/61 (48%), Gaps = 4/61 (6%)
Frame = +1
Query: 678 PPPPPQPEPGTQ----PPATQFPTSQPTSDHAPTTQVSATQASASQVSTTQFVAAKVSSG 733
PPPPP P PG PP PT+ P+S AP +AT S+ + T F + S+
Sbjct: 283 PPPPPSP-PGRSRSATPPPLAAPTASPSSSPAPLASSAATAPLPSKNAATAFSGSTTSTA 459
Query: 734 T 734
T
Sbjct: 460 T 462
>TC207334 similar to UP|Q949K3 (Q949K3) Suppressor-like protein, partial
(53%)
Length = 1828
Score = 40.4 bits (93), Expect = 0.003
Identities = 19/48 (39%), Positives = 25/48 (51%)
Frame = -2
Query: 678 PPPPPQPEPGTQPPATQFPTSQPTSDHAPTTQVSATQASASQVSTTQF 725
P PPPQ P PA+Q TS+P + APT + S + +S T F
Sbjct: 342 PAPPPQTLPSRSEPASQGSTSEPETSAAPTARTSPSTSSPKHTPTPLF 199
>TC214000
Length = 659
Score = 40.4 bits (93), Expect = 0.003
Identities = 19/45 (42%), Positives = 26/45 (57%)
Frame = +3
Query: 640 RRKDVDEKRDEQQLKKAKYGMKCSRCKAEGHNKSTCKLPPPPPQP 684
RR++VDE ++ +L+ C RC GHNK TCK P P+P
Sbjct: 12 RRREVDED*NDHKLRGTNTTN*CWRCHKLGHNKRTCKNSPIHPKP 146
>TC234897 weakly similar to UP|TIR3_YEAST (P40552) Cell wall protein TIR3
precursor, partial (12%)
Length = 698
Score = 39.3 bits (90), Expect = 0.006
Identities = 17/55 (30%), Positives = 28/55 (50%)
Frame = +1
Query: 678 PPPPPQPEPGTQPPATQFPTSQPTSDHAPTTQVSATQASASQVSTTQFVAAKVSS 732
PPPPP P T P+ P P + AP+ ++ A+AS +++ A +S+
Sbjct: 67 PPPPPSRRPSTSAPSAASPLRPPRTSTAPSPSSASASAAASSAASSPSSAPPISA 231
>BE806360 similar to GP|6175165|gb| Mutator-like transposase {Arabidopsis
thaliana}, partial (17%)
Length = 431
Score = 38.5 bits (88), Expect = 0.011
Identities = 16/46 (34%), Positives = 28/46 (60%)
Frame = +2
Query: 336 QGLLPALAELLPDADNRFCVRHLYNNFRKKYPGKKLKELMWRAAKA 381
+G++ + P A + FC++HL ++FRK++ L L+W AA A
Sbjct: 125 KGIVDGVEASFPTAFHGFCMQHLSDSFRKEFNNTMLVNLLWEAANA 262
>TC215592 similar to UP|Q9SSS9 (Q9SSS9) H+-transporting ATP synthase-like
protein, partial (87%)
Length = 961
Score = 38.1 bits (87), Expect = 0.014
Identities = 22/61 (36%), Positives = 27/61 (44%), Gaps = 9/61 (14%)
Frame = +2
Query: 671 NKSTCKLPPPPPQPEPGTQPPATQFP---------TSQPTSDHAPTTQVSATQASASQVS 721
+ S LPPPPP P P PPA + P T +P+ P T S Q S+ S
Sbjct: 137 SSSNSALPPPPPPPPPPAAPPAPREPECQLPQPPATPRPSPTWRPRTTPSTRQPRTSRKS 316
Query: 722 T 722
T
Sbjct: 317 T 319
>TC223428 similar to UP|Q8VZE1 (Q8VZE1) At2g17250/T23A1.11, partial (22%)
Length = 635
Score = 38.1 bits (87), Expect = 0.014
Identities = 23/74 (31%), Positives = 35/74 (47%)
Frame = +2
Query: 663 SRCKAEGHNKSTCKLPPPPPQPEPGTQPPATQFPTSQPTSDHAPTTQVSATQASASQVST 722
SR S LPPPPP P PPAT P+S + P++ S++ +S S +
Sbjct: 152 SRSTPSSSLSSPSSLPPPPPP--PPLPPPATTNPSSSTSPGSVPSSTSSSSPSSTSSLRR 325
Query: 723 TQFVAAKVSSGTQS 736
+ ++ S T+S
Sbjct: 326 REMRRSRNSCWTRS 367
>BM892538 similar to GP|9759296|dbj| gamma-tubulin interacting protein-like
{Arabidopsis thaliana}, partial (8%)
Length = 421
Score = 37.7 bits (86), Expect = 0.019
Identities = 18/60 (30%), Positives = 33/60 (55%)
Frame = +2
Query: 671 NKSTCKLPPPPPQPEPGTQPPATQFPTSQPTSDHAPTTQVSATQASASQVSTTQFVAAKV 730
+KS+ K+ PPPQP P + P + P PT + TT S++ + +T++F ++ +
Sbjct: 98 SKSSPKIATPPPQPPPLSSPTSVS-PNLPPTKNPGTTTAPSSSPRTPRTAATSRFASSSI 274
>TC228693 similar to UP|Q8S9A6 (Q8S9A6) Glucosyltransferase-3, partial (59%)
Length = 907
Score = 37.7 bits (86), Expect = 0.019
Identities = 17/33 (51%), Positives = 19/33 (57%)
Frame = +1
Query: 676 KLPPPPPQPEPGTQPPATQFPTSQPTSDHAPTT 708
K PPPPP P P T PP T P S P +P+T
Sbjct: 205 KTPPPPPPPSPATPPPNTS-PPSPPPPPPSPST 300
Score = 32.0 bits (71), Expect = 1.0
Identities = 20/59 (33%), Positives = 27/59 (44%), Gaps = 5/59 (8%)
Frame = +1
Query: 671 NKSTCKLPPPPPQP---EPGTQPPATQFPTSQPTSDHAP--TTQVSATQASASQVSTTQ 724
N S PPPPP P P + P P P+S AP TT V+++ S ++ Q
Sbjct: 253 NTSPPSPPPPPPSPSTASPRSPSPPFSLPWPSPSSSAAPPATTSVASSTPSPKPQTSKQ 429
>TC218974 weakly similar to UP|PCD7_MOUSE (Q9WTY1) Programmed cell death
protein 7 (ES18), partial (7%)
Length = 890
Score = 37.7 bits (86), Expect = 0.019
Identities = 19/43 (44%), Positives = 22/43 (50%)
Frame = +2
Query: 674 TCKLPPPPPQPEPGTQPPATQFPTSQPTSDHAPTTQVSATQAS 716
TC LPPPPP P P PPA + +S PTT S +S
Sbjct: 170 TCPLPPPPPLPPPPRPPPARPY-----SSTAGPTTATSRRTSS 283
>BF595364
Length = 418
Score = 37.4 bits (85), Expect = 0.024
Identities = 17/62 (27%), Positives = 29/62 (46%)
Frame = +1
Query: 678 PPPPPQPEPGTQPPATQFPTSQPTSDHAPTTQVSATQASASQVSTTQFVAAKVSSGTQSV 737
PPPPP P P+ PT+ S ++PT + S++ + T + + GT S+
Sbjct: 166 PPPPPSSTPXNPAPSPSPPTTSKKSSNSPTASPARPSNSSAGAAAT*TTTTRPTPGTSSL 345
Query: 738 AA 739
+
Sbjct: 346 TS 351
>TC225689 homologue to UP|RSP4_SOYBN (O22518) 40S ribosomal protein SA (p40),
complete
Length = 1265
Score = 37.0 bits (84), Expect = 0.032
Identities = 20/52 (38%), Positives = 26/52 (49%), Gaps = 4/52 (7%)
Frame = +1
Query: 678 PPPPPQPEPGTQPPATQFPTSQ----PTSDHAPTTQVSATQASASQVSTTQF 725
PPPP P+P + PTS+ PTS AP T S ++S +TT F
Sbjct: 82 PPPPTPPQPRRASSPRRKPTSR*C*PPTSTSAPKTATSKWNVTSSSAATTGF 237
>BG043254
Length = 421
Score = 37.0 bits (84), Expect = 0.032
Identities = 17/46 (36%), Positives = 27/46 (57%)
Frame = +2
Query: 678 PPPPPQPEPGTQPPATQFPTSQPTSDHAPTTQVSATQASASQVSTT 723
PPPPP+P P + PP P PTS + T+ + ++SA+ S++
Sbjct: 77 PPPPPRPLPQSHPPP---PPPSPTSKTSTTSSSQSPKSSAASASSS 205
Score = 31.6 bits (70), Expect = 1.3
Identities = 23/61 (37%), Positives = 30/61 (48%), Gaps = 1/61 (1%)
Frame = +2
Query: 677 LPP-PPPQPEPGTQPPATQFPTSQPTSDHAPTTQVSATQASASQVSTTQFVAAKVSSGTQ 735
LPP PP P P +P P P S PT++ S T +S S S+ AA SS ++
Sbjct: 53 LPPLRPPHPPPPPRPLPQSHPPPPPPS---PTSKTSTTSSSQSPKSS----AASASSSSR 211
Query: 736 S 736
S
Sbjct: 212 S 214
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.342 0.148 0.499
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,284,385
Number of Sequences: 63676
Number of extensions: 670371
Number of successful extensions: 18543
Number of sequences better than 10.0: 422
Number of HSP's better than 10.0 without gapping: 9886
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 14528
length of query: 739
length of database: 12,639,632
effective HSP length: 104
effective length of query: 635
effective length of database: 6,017,328
effective search space: 3821003280
effective search space used: 3821003280
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (22.0 bits)
S2: 63 (28.9 bits)
Medicago: description of AC122730.8