BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122727.10 - phase: 1 /pseudo
(136 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC212986 50 4e-07
BM108247 weakly similar to PIR|H84710|H847 Mutator-like transpos... 39 6e-04
TC228067 32 0.093
TC228324 UP|LGUL_SOYBN (P46417) Lactoylglutathione lyase (Methy... 32 0.12
TC228281 similar to UP|BIOB_ARATH (P54967) Biotin synthase (Bio... 30 0.46
TC205889 UP|Q9FQE9 (Q9FQE9) Glutathione S-transferase GST 9 , c... 28 1.3
TC214794 homologue to UP|HMGL_SOYBN (P26585) HMG1/2-like protein... 27 2.3
TC214076 homologue to UP|HMGL_SOYBN (P26585) HMG1/2-like protein... 27 2.3
TC232184 weakly similar to UP|Q70KS2 (Q70KS2) BSD protein (Fragm... 27 2.3
AW101164 27 3.9
TC207488 similar to UP|Q9SJE0 (Q9SJE0) T27G7.21, partial (58%) 27 3.9
TC228023 similar to UP|Q9FLH2 (Q9FLH2) 2-oxoglutarate dehydrogen... 27 3.9
AW620948 26 5.1
BI785278 26 5.1
BG790084 similar to GP|24421095|dbj receptor-like protein kinase... 26 6.6
TC216468 homologue to UP|Q9FZE1 (Q9FZE1) T1K7.6 protein (At1g265... 25 8.7
TC228030 similar to UP|Q8LPS4 (Q8LPS4) At3g06670/T8E24.10, parti... 25 8.7
TC210267 similar to UP|Q8LGU6 (Q8LGU6) HOBBIT protein, partial (... 25 8.7
>TC212986
Length = 404
Score = 49.7 bits (117), Expect = 4e-07
Identities = 22/53 (41%), Positives = 35/53 (65%), Gaps = 2/53 (3%)
Frame = +1
Query: 68 HYRN--QIRDWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNNVLK 118
H+R+ IR +W+D PK++W+Q D+G+RWG +TTN+SE N++ K
Sbjct: 1 HWRHLGDIRANKPSAAEWLDQLPKQKWVQCFDEGKRWG-LTTNLSESVNSMFK 156
>BM108247 weakly similar to PIR|H84710|H847 Mutator-like transposase
[imported] - Arabidopsis thaliana, partial (4%)
Length = 265
Score = 39.3 bits (90), Expect = 6e-04
Identities = 17/66 (25%), Positives = 32/66 (47%)
Frame = +2
Query: 46 KNTEMKKKVRNMGYSMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWLQAHDQGRRWGHI 105
KN+ + + ++ T + +I + S + KW+ QW H +G R+GH+
Sbjct: 2 KNSRLVHLLWKASHATTTIAFKEKMGEIEEVSPEAAKWLQQFXPSQWALVHFKGTRFGHL 181
Query: 106 TTNISE 111
++NI E
Sbjct: 182 SSNIEE 199
>TC228067
Length = 1716
Score = 32.0 bits (71), Expect = 0.093
Identities = 23/63 (36%), Positives = 29/63 (45%), Gaps = 9/63 (14%)
Frame = +1
Query: 81 VKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNNV--LKG-----GPQSSNHTN--CPG 131
V+WV D + LQ HD R G ++ + KG GP S NHT+ CPG
Sbjct: 490 VEWVKDQIRANGLQMHDNVLRKGINPRTRAQQLEDFRRFKGISHYEGPDSDNHTSLPCPG 669
Query: 132 NLL 134
LL
Sbjct: 670 ELL 678
>TC228324 UP|LGUL_SOYBN (P46417) Lactoylglutathione lyase (Methylglyoxalase)
(Aldoketomutase) (Glyoxalase I) , complete
Length = 995
Score = 31.6 bits (70), Expect = 0.12
Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 3/45 (6%)
Frame = +1
Query: 60 SMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWL---QAHDQGRR 101
S PS + R+Q R W D + K + D KK WL + H++G++
Sbjct: 313 SPLMPSDPYKRSQARFWVDYIDKKIYDTWKKMWLSKGEEHEEGKK 447
>TC228281 similar to UP|BIOB_ARATH (P54967) Biotin synthase (Biotin
synthetase) , partial (39%)
Length = 677
Score = 29.6 bits (65), Expect = 0.46
Identities = 18/56 (32%), Positives = 25/56 (44%)
Frame = +2
Query: 75 DWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNNVLKGGPQSSNHTNCP 130
DWS D VK + D+P L Q R H + +C +K G S + + CP
Sbjct: 299 DWSRDQVKSIYDSPILDLLFHGAQVHRHAHNFREVQQCTLLSIKTGGCSEDCSYCP 466
>TC205889 UP|Q9FQE9 (Q9FQE9) Glutathione S-transferase GST 9 , complete
Length = 1011
Score = 28.1 bits (61), Expect = 1.3
Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 3/41 (7%)
Frame = +3
Query: 64 PSITHYRNQIRDWSDDVVKWVDDNPKKQWL---QAHDQGRR 101
PS + R Q R W D + K V D +K WL + H+ G++
Sbjct: 333 PSDPYERAQARFWVDYIDKKVYDTWRKMWLSKGEEHEAGKK 455
>TC214794 homologue to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB11
protein), complete
Length = 917
Score = 27.3 bits (59), Expect = 2.3
Identities = 21/66 (31%), Positives = 30/66 (44%), Gaps = 6/66 (9%)
Frame = +1
Query: 50 MKKKVRN-----MGYSMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWLQAHDQGRRW-G 103
MKK +R+ M MT+ ++ R IR+W+DD +V + D RW G
Sbjct: 475 MKKNLRSLYLR*MMRMMTRKAVARRRMTIREWTDDNSIYVGSDD-------FDHPCRWSG 633
Query: 104 HITTNI 109
H NI
Sbjct: 634 HFLINI 651
>TC214076 homologue to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB11
protein), complete
Length = 1064
Score = 27.3 bits (59), Expect = 2.3
Identities = 21/66 (31%), Positives = 30/66 (44%), Gaps = 6/66 (9%)
Frame = +2
Query: 50 MKKKVRN-----MGYSMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWLQAHDQGRRW-G 103
MKK +R+ M MT+ ++ R IR+W+DD +V + D RW G
Sbjct: 626 MKKNLRSLYLR*MMRMMTRKAVARRRMTIREWTDDNSIYVGSDD-------FDHPCRWSG 784
Query: 104 HITTNI 109
H NI
Sbjct: 785 HFLINI 802
>TC232184 weakly similar to UP|Q70KS2 (Q70KS2) BSD protein (Fragment),
partial (9%)
Length = 597
Score = 27.3 bits (59), Expect = 2.3
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +2
Query: 42 LRTFKNTEMKKKVRNMGYSMTQPSITHYRNQIRDWSD 78
L TFKN ++ +V TQ S T RN++ W +
Sbjct: 326 LDTFKNFPLQDEVEGSYGEDTQSSSTKVRNELSQWQE 436
>AW101164
Length = 415
Score = 26.6 bits (57), Expect = 3.9
Identities = 15/59 (25%), Positives = 25/59 (41%), Gaps = 2/59 (3%)
Frame = -3
Query: 32 SSAFDT*LNFLRTFKNTEMKKKVRNMGYSMTQPSITHYR--NQIRDWSDDVVKWVDDNP 88
S+ T +FL + KK + PS+T++ N+ W D + +W NP
Sbjct: 359 STLSSTSYSFLHIKQQNHSSKKSSVYDSRLLPPSLTYFYKPNRQNQWKDLI*RWFSQNP 183
>TC207488 similar to UP|Q9SJE0 (Q9SJE0) T27G7.21, partial (58%)
Length = 955
Score = 26.6 bits (57), Expect = 3.9
Identities = 11/44 (25%), Positives = 22/44 (50%), Gaps = 4/44 (9%)
Frame = -3
Query: 76 WSDDVVKWVDD----NPKKQWLQAHDQGRRWGHITTNISECFNN 115
WSD + + ++ N L+ + R+WG ++ + EC+ N
Sbjct: 224 WSDSLGEGAEEQYATNGNADDLELEEMKRKWGCLSVKVEECWYN 93
>TC228023 similar to UP|Q9FLH2 (Q9FLH2) 2-oxoglutarate dehydrogenase, E1
component, partial (36%)
Length = 1591
Score = 26.6 bits (57), Expect = 3.9
Identities = 15/44 (34%), Positives = 19/44 (43%), Gaps = 6/44 (13%)
Frame = -3
Query: 93 LQAHDQGRRWGHITTNISECFNNVL------KGGPQSSNHTNCP 130
L H G+ W +I TN CF+N G Q N T+ P
Sbjct: 1508 LPRHASGK-WNYIVTNYGRCFSNYTA*IKKKNGNSQGINSTSKP 1380
>AW620948
Length = 442
Score = 26.2 bits (56), Expect = 5.1
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = +3
Query: 39 LNFLRTFKNTEMKKKVRNM 57
+N LRT KNTE+++K R +
Sbjct: 48 VNHLRTHKNTEIQRKARGL 104
>BI785278
Length = 422
Score = 26.2 bits (56), Expect = 5.1
Identities = 10/25 (40%), Positives = 13/25 (52%)
Frame = -1
Query: 99 GRRWGHITTNISECFNNVLKGGPQS 123
G+ WGH +C L+GGP S
Sbjct: 326 GQEWGHGLLPRIQCLTKQLQGGPNS 252
>BG790084 similar to GP|24421095|dbj receptor-like protein kinase
{Nicotiana tabacum}, partial (8%)
Length = 397
Score = 25.8 bits (55), Expect = 6.6
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +1
Query: 39 LNFLRTFKNTEMKKKVRNMGYSMTQPSITHYRN 71
LNFL+ F N E N+ PS+ +RN
Sbjct: 10 LNFLKYFPNLETLSVADNLFTGRVPPSVRSFRN 108
>TC216468 homologue to UP|Q9FZE1 (Q9FZE1) T1K7.6 protein (At1g26570/T1K7_6),
partial (25%)
Length = 574
Score = 25.4 bits (54), Expect = 8.7
Identities = 11/27 (40%), Positives = 12/27 (43%)
Frame = +1
Query: 78 DDVVKWVDDNPKKQWLQAHDQGRRWGH 104
DD V WV P+ W G WGH
Sbjct: 466 DDCV-WVKGGPESGWGPGRAVGNSWGH 543
>TC228030 similar to UP|Q8LPS4 (Q8LPS4) At3g06670/T8E24.10, partial (12%)
Length = 1344
Score = 25.4 bits (54), Expect = 8.7
Identities = 10/25 (40%), Positives = 16/25 (64%), Gaps = 1/25 (4%)
Frame = +3
Query: 70 RNQIR-DWSDDVVKWVDDNPKKQWL 93
+NQI +W + V W+ NP ++WL
Sbjct: 612 QNQIAYNWVEKNVHWLPQNPHQRWL 686
>TC210267 similar to UP|Q8LGU6 (Q8LGU6) HOBBIT protein, partial (39%)
Length = 1273
Score = 25.4 bits (54), Expect = 8.7
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = -3
Query: 59 YSMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQW 92
+S P++ YR+ + W V+ + ++PKK W
Sbjct: 1178 FSSFGPALGIYRSFLAPWLHHVLLLICESPKKLW 1077
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.329 0.139 0.467
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,389,224
Number of Sequences: 63676
Number of extensions: 103063
Number of successful extensions: 531
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 529
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 530
length of query: 136
length of database: 12,639,632
effective HSP length: 87
effective length of query: 49
effective length of database: 7,099,820
effective search space: 347891180
effective search space used: 347891180
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC122727.10