BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122725.8 - phase: 0 /pseudo
(297 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC227560 similar to UP|RRF_DAUCA (P37706) Ribosome recycling fac... 340 5e-94
TC227561 similar to UP|RRF_DAUCA (P37706) Ribosome recycling fac... 269 1e-72
BF424844 weakly similar to SP|Q9M1X0|RRF_ Ribosome recycling fac... 199 2e-51
BF070508 weakly similar to SP|Q9M1X0|RRF_A Ribosome recycling fa... 110 1e-24
BE822100 62 2e-10
AW186460 40 0.002
AW756794 36 0.024
TC206134 31 0.77
TC210374 28 3.8
TC229683 similar to GB|AAM62553.1|21553460|AY085322 snap25a {Ara... 28 5.0
CB063582 28 5.0
TC206856 28 6.5
TC210783 weakly similar to UP|O14760 (O14760) Small intestinal m... 27 8.5
>TC227560 similar to UP|RRF_DAUCA (P37706) Ribosome recycling factor,
chloroplast precursor (Ribosome releasing factor,
chloroplast) (Nuclear located protein D2) (Fragment),
partial (90%)
Length = 1106
Score = 340 bits (872), Expect = 5e-94
Identities = 196/295 (66%), Positives = 218/295 (73%), Gaps = 5/295 (1%)
Frame = +3
Query: 1 MATSFSSTTPLRSI-FQNLPIVPKALLSAHSSFTIKRSSTYVTLKLSG----SGRHSLSK 55
MATSFS T + SI FQN P L + S++Y TLKL S R LSK
Sbjct: 81 MATSFSPTASVGSIIFQNPPKGLLLLSQQQQQRSFASSASYATLKLPRAFALSPRPLLSK 260
Query: 56 GLLTLHTRYQNYYSRSACFQF*TTSLVFIVICFF*KIPRKGFVRAATIEEIEAEKASIEN 115
G TL VRAATIEEIEAEKA+IE
Sbjct: 261 GRTTL-------------------------------------VRAATIEEIEAEKAAIEK 329
Query: 116 DVKSRMERTLDNVKTNFSSIRTGRATPSMLDKIEVEYYGSPVSLKTIAQISTPDASSLLV 175
D K+RM+RT+DNV+TNFSSIRTGRA PSMLDKI+VEYYGSPVSLK+IAQISTPDASSLLV
Sbjct: 330 DAKTRMDRTIDNVRTNFSSIRTGRANPSMLDKIQVEYYGSPVSLKSIAQISTPDASSLLV 509
Query: 176 SPYDKSSLKAIEKAIVNSDVGMTPNNDGEVIRLSIPQLTSDRRKELTKIVSKQVEEGKVA 235
PYDKSSLKAIEKAIV+SD+GMTPNNDGE+IRLSIPQLTSDRRKEL+KIV+KQ EEGKVA
Sbjct: 510 QPYDKSSLKAIEKAIVSSDLGMTPNNDGELIRLSIPQLTSDRRKELSKIVAKQAEEGKVA 689
Query: 236 LRNIRRDALKAYEKLEKDKKLSEDNVKDLSSDLQKLTDDYIKKVDALYKQKEKVL 290
LRNIRRDALKAY+KLEK+KKLSEDNVKDLSSDLQKLTD+Y+KKVD ++KQKEK L
Sbjct: 690 LRNIRRDALKAYDKLEKEKKLSEDNVKDLSSDLQKLTDEYMKKVDTIFKQKEKEL 854
>TC227561 similar to UP|RRF_DAUCA (P37706) Ribosome recycling factor,
chloroplast precursor (Ribosome releasing factor,
chloroplast) (Nuclear located protein D2) (Fragment),
partial (71%)
Length = 723
Score = 269 bits (688), Expect = 1e-72
Identities = 140/173 (80%), Positives = 155/173 (88%)
Frame = +2
Query: 118 KSRMERTLDNVKTNFSSIRTGRATPSMLDKIEVEYYGSPVSLKTIAQISTPDASSLLVSP 177
K+RM+RT+DNV+TNFSSIRTGRA PSMLDKI+VEYYGSPVSLK+IAQISTPDASSLLV P
Sbjct: 5 KTRMDRTIDNVRTNFSSIRTGRANPSMLDKIQVEYYGSPVSLKSIAQISTPDASSLLVQP 184
Query: 178 YDKSSLKAIEKAIVNSDVGMTPNNDGEVIRLSIPQLTSDRRKELTKIVSKQVEEGKVALR 237
YDKSSLKAIEKAIV+SD+GMTPNNDGE+IRLSIPQLTSDRR KVALR
Sbjct: 185 YDKSSLKAIEKAIVSSDLGMTPNNDGELIRLSIPQLTSDRR--------------KVALR 322
Query: 238 NIRRDALKAYEKLEKDKKLSEDNVKDLSSDLQKLTDDYIKKVDALYKQKEKVL 290
NIRRDALKAY+KLEK+KKLSEDNVKDLSSDLQKLTD+Y+KKVD ++KQKEK L
Sbjct: 323 NIRRDALKAYDKLEKEKKLSEDNVKDLSSDLQKLTDEYMKKVDTIFKQKEKEL 481
>BF424844 weakly similar to SP|Q9M1X0|RRF_ Ribosome recycling factor
chloroplast precursor (Ribosome releasing factor
chloroplast) (RRF), partial (46%)
Length = 484
Score = 199 bits (505), Expect = 2e-51
Identities = 105/157 (66%), Positives = 125/157 (78%)
Frame = +2
Query: 94 RKGFVRAATIEEIEAEKASIENDVKSRMERTLDNVKTNFSSIRTGRATPSMLDKIEVEYY 153
R RAA IEEI A+KA+ + D ++ M+ T+DNV+T F SI TGRA PSMLDKI VEYY
Sbjct: 14 RAALERAAAIEEIVADKAAFDKDAQTSMDWTIDNVRTYFISIMTGRAIPSMLDKIHVEYY 193
Query: 154 GSPVSLKTIAQISTPDASSLLVSPYDKSSLKAIEKAIVNSDVGMTPNNDGEVIRLSIPQL 213
GSPVSLK+IA IS+PDA SLLV PYDKS LKAIEKAIV+SD+GMTPN+DG +IRL IPQL
Sbjct: 194 GSPVSLKSIAHISSPDAISLLVQPYDKSCLKAIEKAIVSSDLGMTPNHDGYLIRLIIPQL 373
Query: 214 TSDRRKELTKIVSKQVEEGKVALRNIRRDALKAYEKL 250
TSDR +L IV++ E G VAL NIRRDAL+ Y++L
Sbjct: 374 TSDRTNDL**IVAQPAEHGMVALLNIRRDALEPYDRL 484
>BF070508 weakly similar to SP|Q9M1X0|RRF_A Ribosome recycling factor
chloroplast precursor (Ribosome releasing factor
chloroplast) (RRF), partial (31%)
Length = 355
Score = 110 bits (274), Expect = 1e-24
Identities = 59/93 (63%), Positives = 69/93 (73%)
Frame = +2
Query: 94 RKGFVRAATIEEIEAEKASIENDVKSRMERTLDNVKTNFSSIRTGRATPSMLDKIEVEYY 153
R V AA IE IEAE A IE D K+RM+RT+DNV+TNF SI TGRA PSMLDKI+V YY
Sbjct: 77 RTTLVTAAPIE*IEAE*AVIEKDAKTRMDRTIDNVRTNFISITTGRANPSMLDKIQVVYY 256
Query: 154 GSPVSLKTIAQISTPDASSLLVSPYDKSSLKAI 186
GSP LK+I +I+TP A +LL PY +S KAI
Sbjct: 257 GSPACLKSITKITTPKAIALLAQPY*RSYSKAI 355
>BE822100
Length = 667
Score = 62.4 bits (150), Expect = 2e-10
Identities = 34/103 (33%), Positives = 59/103 (57%)
Frame = -2
Query: 186 IEKAIVNSDVGMTPNNDGEVIRLSIPQLTSDRRKELTKIVSKQVEEGKVALRNIRRDALK 245
+E A V+S G+ +DGE + I LT + + +TK+V+K E+ + ++R R+ A+
Sbjct: 660 LENAXVSSPXGLXXXSDGERLIAVIXXLTKEHMQAMTKLVAKSCEDARQSIRRARQKAMD 481
Query: 246 AYEKLEKDKKLSEDNVKDLSSDLQKLTDDYIKKVDALYKQKEK 288
A +KL L +D++K L ++ LT +IK + + K KEK
Sbjct: 480 AIKKLY--SSLPKDDIKRLEKEVDDLTKKFIKSAEDICKAKEK 358
>AW186460
Length = 442
Score = 39.7 bits (91), Expect = 0.002
Identities = 22/52 (42%), Positives = 31/52 (59%)
Frame = +3
Query: 100 AATIEEIEAEKASIENDVKSRMERTLDNVKTNFSSIRTGRATPSMLDKIEVE 151
A+TIE +I+ + S+ME + + T S +RTGRA+P MLD I VE
Sbjct: 282 ASTIEVPPNAGPTIKANAVSQMEAAMAALSTELSKLRTGRASPGMLDHIIVE 437
>AW756794
Length = 391
Score = 35.8 bits (81), Expect = 0.024
Identities = 18/23 (78%), Positives = 19/23 (82%)
Frame = +3
Query: 94 RKGFVRAATIEEIEAEKASIEND 116
R VRAATIEEIEAEKA+IE D
Sbjct: 255 RTPLVRAATIEEIEAEKAAIEKD 323
Score = 34.7 bits (78), Expect = 0.053
Identities = 37/101 (36%), Positives = 46/101 (44%), Gaps = 13/101 (12%)
Frame = +1
Query: 2 ATSFSSTTPLRSIFQNLPIVPKALLSAHSSFTIKRSSTYVTLKLSGSGRHSLSKG----- 56
+TSFS T +RSI PKALL + S++Y TLKL R LSKG
Sbjct: 97 STSFSPTASVRSIIFQKQNPPKALLLLSQQRSFASSASYATLKL--PRRPLLSKGERHL* 270
Query: 57 --LLTLHTRYQNYYSR------SACFQF*TTSLVFIVICFF 89
LL+ + + SR S C TSL F V+ FF
Sbjct: 271 GLLLSKKLKPKKQQSRKML**TSFCL---*TSLSFCVLQFF 384
>TC206134
Length = 789
Score = 30.8 bits (68), Expect = 0.77
Identities = 32/130 (24%), Positives = 59/130 (44%), Gaps = 7/130 (5%)
Frame = +3
Query: 158 SLKTIAQISTPDASSLLVSPYDKSSLKAIEKAIVNSDVGMTPNNDGEVIRLSIPQLTSDR 217
SL + + P+ASS L P K SL + + +S V ++ + +R +P+ TS
Sbjct: 12 SLMSTTLSNLPNASSFLPKPKPKPSLPHPQTPLTHSAVLLSRSI---CLRNLLPKATS-- 176
Query: 218 RKELTKIVSKQVEEGKVALRNIRRDALKAYEKLEKDKK-------LSEDNVKDLSSDLQK 270
S++ G N +RD + E +++D K + + ++LS D Q
Sbjct: 177 --------SEERSTGGSQFFNEKRDGVIILENVKEDNKNEFDKTVIEDTKEEELSDDGQG 332
Query: 271 LTDDYIKKVD 280
L+ D + K++
Sbjct: 333 LSFDLMDKLN 362
>TC210374
Length = 859
Score = 28.5 bits (62), Expect = 3.8
Identities = 32/144 (22%), Positives = 58/144 (40%), Gaps = 7/144 (4%)
Frame = +3
Query: 124 TLDNVKTNFSSIRTGRATPSMLDKIEVEYYGSPVSLKTIAQISTPDASSLLVSPYDKSSL 183
T ++ TN S + + DK+ ++ +P ++ ST +P +K
Sbjct: 222 TKPSISTNLPSDPKNKHATPLADKVPLKQDVNPTKELQVSNPST--------TPKEKLQE 377
Query: 184 KAIEKAIVNSDVGMTPNNDGEVIR---LSIPQLTSDRRKELTKIVSKQ----VEEGKVAL 236
K S V P G + + SIP + +E++K + Q E+G V
Sbjct: 378 NEPPKVTTGSQVDSNPEK-GRLEKGQETSIPLVKISGGEEVSKDKANQGVVCEEQGSVKK 554
Query: 237 RNIRRDALKAYEKLEKDKKLSEDN 260
+ + K + LE+DKK++ N
Sbjct: 555 ATEKNNIDKGSQSLEQDKKINSIN 626
>TC229683 similar to GB|AAM62553.1|21553460|AY085322 snap25a {Arabidopsis
thaliana;} , partial (66%)
Length = 902
Score = 28.1 bits (61), Expect = 5.0
Identities = 14/41 (34%), Positives = 25/41 (60%)
Frame = +2
Query: 231 EGKVALRNIRRDALKAYEKLEKDKKLSEDNVKDLSSDLQKL 271
+G+ LR+ ++ A+EK+E +K +D + DLS L +L
Sbjct: 368 KGQSKLRSPPQEPTNAFEKVEVEKNKQDDALSDLSDLLGEL 490
>CB063582
Length = 401
Score = 28.1 bits (61), Expect = 5.0
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = -1
Query: 62 TRYQNYYSRSACFQF*TTSL 81
T Y NY+ RS+CF + +TS+
Sbjct: 86 TNYNNYFYRSSCFTYPSTSI 27
>TC206856
Length = 428
Score = 27.7 bits (60), Expect = 6.5
Identities = 18/47 (38%), Positives = 25/47 (52%)
Frame = -3
Query: 32 FTIKRSSTYVTLKLSGSGRHSLSKGLLTLHTRYQNYYSRSACFQF*T 78
F+I RSS+ V+ SGS R+ + L L + QN Y CF+ T
Sbjct: 165 FSISRSSSLVSCVSSGSTRNGVLYCLCRLMPK-QNGYGNGKCFRLTT 28
>TC210783 weakly similar to UP|O14760 (O14760) Small intestinal mucin MUC3
(Fragment), partial (5%)
Length = 735
Score = 27.3 bits (59), Expect = 8.5
Identities = 26/91 (28%), Positives = 38/91 (41%)
Frame = -3
Query: 3 TSFSSTTPLRSIFQNLPIVPKALLSAHSSFTIKRSSTYVTLKLSGSGRHSLSKGLLTLHT 62
TSF+S+TP +F P +LLS+ +S T T LS + S L + +
Sbjct: 586 TSFTSSTPSSFLFSFASSTPSSLLSSFTSST--------TSFLSSLASFTPSSFLSSFAS 431
Query: 63 RYQNYYSRSACFQF*TTSLVFIVICFF*KIP 93
++ S+ F T L I F IP
Sbjct: 430 STTPFFLSSSTFSTPTAFLSSISFTIFFLIP 338
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.133 0.359
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,969,739
Number of Sequences: 63676
Number of extensions: 118425
Number of successful extensions: 627
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 618
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 625
length of query: 297
length of database: 12,639,632
effective HSP length: 97
effective length of query: 200
effective length of database: 6,463,060
effective search space: 1292612000
effective search space used: 1292612000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC122725.8