BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122166.8 + phase: 0
(404 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC216434 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Frag... 479 e-136
TC216436 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Frag... 208 4e-54
TC216435 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Frag... 167 8e-42
AW278727 similar to PIR|T06227|T06 peroxidase (EC 1.11.1.7) 2 c... 31 1.1
TC225212 UP|O64970 (O64970) Cationic peroxidase 2 , complete 30 2.0
BE191314 30 2.0
TC214928 UP|Q9XFI6 (Q9XFI6) Peroxidase, complete 30 2.0
TC203568 weakly similar to UP|RLA1_MAIZE (P52855) 60S acidic rib... 30 2.0
TC225213 UP|O64970 (O64970) Cationic peroxidase 2 , partial (64%) 30 2.0
CD392208 weakly similar to PIR|T02039|T020 acidic ribosomal prot... 30 2.6
BF008872 29 3.3
TC224879 homologue to gb|AF223066.1|AF223066 Humulus lupulus 26S... 29 4.4
TC223205 29 4.4
TC222372 similar to UP|Q7KS14 (Q7KS14) CG6695-PB, partial (5%) 28 5.7
TC212974 weakly similar to UP|COM3_POPKI (Q43047) Caffeic acid 3... 28 7.4
TC204381 similar to UP|CRTC_RICCO (P93508) Calreticulin precurso... 28 7.4
>TC216434 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Fragment),
partial (96%)
Length = 1436
Score = 479 bits (1233), Expect = e-136
Identities = 249/387 (64%), Positives = 304/387 (78%), Gaps = 1/387 (0%)
Frame = +1
Query: 16 KSLTNLTNVSLNITKHLLSNQKLNEKNVVFSPLSLNTVLIMITAGSEGPTQNQLLSFLQS 75
+S++N T+V+L+I+K LLS + +KN+V+SPLSL+ VL +I AGS+GPT +QLLSFL+S
Sbjct: 22 ESISNQTDVALSISKLLLSKEA-RDKNLVYSPLSLHVVLSIIAAGSKGPTLDQLLSFLRS 198
Query: 76 ESTGDLKSLCSQLVSSVLSDGAPAGGPCLSHVNGVWVEQSLPLQPSFKQLMTTDFKATLA 135
+ST L S SQL + VLSD +PAGGP LS +GVWVEQSL L PSFKQL++ D+KATLA
Sbjct: 199 KSTDHLNSFASQLFAVVLSDASPAGGPRLSFADGVWVEQSLSLLPSFKQLVSADYKATLA 378
Query: 136 AVDFINKADEVIKEVNLWANKETKGFINDLLPRGSVDSLTSLIFANALYFKGVWKRPFDT 195
+VDF KA EV EVN WA KET G + DLLP GSVDS T LIFANALYFKG W FD+
Sbjct: 379 SVDFQTKAVEVANEVNSWAEKETNGLVKDLLPPGSVDSSTRLIFANALYFKGAWNEKFDS 558
Query: 196 SKTKDYDFDLLNGKSVKVPFMTSKNNQFISSFDGFKVLGLPYKQGNDKRAFSIYFFLPDE 255
S TKDYDF LL+G+S++VPFMTS+ NQFI +FDGFKVLGLPYKQG DKR F++YFFLP+
Sbjct: 559 SITKDYDFHLLDGRSIRVPFMTSRKNQFIRAFDGFKVLGLPYKQGEDKRQFTMYFFLPET 738
Query: 256 KDGLSALIDKVASDSEFLEQKLPRNQVKVGKFMIPRFNISFEIEASELLNKLGLTLPFSK 315
KDGL AL +K+AS+S FLE+KLP N+++VG F IPRF ISF EAS +L +LG+ LPFS
Sbjct: 739 KDGLLALAEKLASESGFLERKLPNNKLEVGDFRIPRFKISFGFEASNVLKELGVVLPFSV 918
Query: 316 GGLTKMVDSPISQELSVTSIFQKSFIELNEEGT-IAAATARGSTGGAAPFRLPPPPPIDF 374
GGLT+MVDS + Q L V+ IF KSFIE+NEEGT AAATA G A F P IDF
Sbjct: 919 GGLTEMVDSAVGQNLFVSDIFHKSFIEVNEEGTEAAAATAATIQFGCAMF----PTEIDF 1086
Query: 375 VADHPFLFLIREEFSGTILFVGKVVNP 401
VADHPFLFLIRE+ +GT+LF+G+V+NP
Sbjct: 1087VADHPFLFLIREDLTGTVLFIGQVLNP 1167
>TC216436 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Fragment),
partial (40%)
Length = 489
Score = 208 bits (529), Expect = 4e-54
Identities = 105/161 (65%), Positives = 120/161 (74%)
Frame = +2
Query: 118 LQPSFKQLMTTDFKATLAAVDFINKADEVIKEVNLWANKETKGFINDLLPRGSVDSLTSL 177
L PSFKQL+ +KATLA+VDF KA EV EVN WA KET G + DLLP GSVD+ T L
Sbjct: 5 LHPSFKQLVRAQYKATLASVDFQTKAVEVTNEVNSWAEKETNGLVKDLLPPGSVDNSTKL 184
Query: 178 IFANALYFKGVWKRPFDTSKTKDYDFDLLNGKSVKVPFMTSKNNQFISSFDGFKVLGLPY 237
IFANAL+ KG W FD S TK YD LLNG SVKVPFMTSK QFI + + FKVLGL Y
Sbjct: 185 IFANALWCKGAWNEKFDASITKHYDLHLLNGSSVKVPFMTSKKKQFIMA*NSFKVLGLSY 364
Query: 238 KQGNDKRAFSIYFFLPDEKDGLSALIDKVASDSEFLEQKLP 278
KQG DKR F++YFFLP+ KDGL AL +K+A DS FLE++LP
Sbjct: 365 KQGEDKRQFTMYFFLPETKDGLLALAEKLAFDSGFLERRLP 487
>TC216435 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Fragment),
partial (37%)
Length = 820
Score = 167 bits (423), Expect = 8e-42
Identities = 89/151 (58%), Positives = 111/151 (72%), Gaps = 3/151 (1%)
Frame = +2
Query: 257 DGLSALIDKVASDSEFLEQKLPRNQVKVGKFMIPRFNISFEIEASELLNKLGLTLPFSKG 316
DGL AL +K+AS+S FLE+KLP +V+VG F IPRF ISF E S +L +LG+ LPFS G
Sbjct: 11 DGLLALAEKLASESGFLERKLPNQKVEVGDFRIPRFKISFGFEVSNVLKELGVVLPFSVG 190
Query: 317 GLTKMVDSPISQELSVTSIFQKSFIELNEEGTIAAATARGSTGGAAPFRLPP---PPPID 373
GLT+MVDSP+ Q L V++IF KSFIE+NEEGT AAA +A RL P ID
Sbjct: 191 GLTEMVDSPVGQNLCVSNIFHKSFIEVNEEGTEAAAAT------SATIRLRSAMLPTKID 352
Query: 374 FVADHPFLFLIREEFSGTILFVGKVVNPLDG 404
FVADHPFLFLIRE+ +GT+LF+G+V++P G
Sbjct: 353 FVADHPFLFLIREDLTGTVLFIGQVLDPRAG 445
>AW278727 similar to PIR|T06227|T06 peroxidase (EC 1.11.1.7) 2 cationic -
soybean, partial (55%)
Length = 556
Score = 30.8 bits (68), Expect = 1.1
Identities = 17/49 (34%), Positives = 25/49 (50%), Gaps = 7/49 (14%)
Frame = +2
Query: 224 ISSFDGFKVLGLPY-------KQGNDKRAFSIYFFLPDEKDGLSALIDK 265
+S+ DG LG P+ G RA + FLPD + +SA++DK
Sbjct: 383 LSAIDGIDTLGGPHIPLKTXRMDGRMSRADVVEQFLPDHNESISAVLDK 529
>TC225212 UP|O64970 (O64970) Cationic peroxidase 2 , complete
Length = 1484
Score = 30.0 bits (66), Expect = 2.0
Identities = 17/49 (34%), Positives = 26/49 (52%), Gaps = 7/49 (14%)
Frame = +1
Query: 224 ISSFDGFKVLGLPY-------KQGNDKRAFSIYFFLPDEKDGLSALIDK 265
+S+ DG LG P+ + G RA + FLPD + +SA++DK
Sbjct: 406 LSARDGIVSLGGPHIPLKTGRRDGRRSRADVVEQFLPDHNESISAVLDK 552
>BE191314
Length = 473
Score = 30.0 bits (66), Expect = 2.0
Identities = 17/49 (34%), Positives = 26/49 (52%), Gaps = 7/49 (14%)
Frame = +2
Query: 224 ISSFDGFKVLGLPY-------KQGNDKRAFSIYFFLPDEKDGLSALIDK 265
+S+ DG LG P+ + G RA + FLPD + +SA++DK
Sbjct: 323 LSARDGIVSLGGPHIPLKTGRRDGRRSRADVVEQFLPDHNESISAVLDK 469
>TC214928 UP|Q9XFI6 (Q9XFI6) Peroxidase, complete
Length = 1545
Score = 30.0 bits (66), Expect = 2.0
Identities = 17/49 (34%), Positives = 26/49 (52%), Gaps = 7/49 (14%)
Frame = +2
Query: 224 ISSFDGFKVLGLPY-------KQGNDKRAFSIYFFLPDEKDGLSALIDK 265
+S+ DG LG P+ + G RA + FLPD + +SA++DK
Sbjct: 506 LSARDGIVSLGGPHIPLKTGRRDGRRSRADVVEQFLPDHNESISAVLDK 652
>TC203568 weakly similar to UP|RLA1_MAIZE (P52855) 60S acidic ribosomal
protein P1 (L12), complete
Length = 680
Score = 30.0 bits (66), Expect = 2.0
Identities = 29/114 (25%), Positives = 37/114 (32%)
Frame = -3
Query: 269 DSEFLEQKLPRNQVKVGKFMIPRFNISFEIEASELLNKLGLTLPFSKGGLTKMVDSPISQ 328
DS + +N ++ K I R I ++ NK P+S
Sbjct: 543 DSRLAKNPFNKNYIEQNKISIQRLKIWLQLYKKI*SNK----------------PKPMSS 412
Query: 329 ELSVTSIFQKSFIELNEEGTIAAATARGSTGGAAPFRLPPPPPIDFVADHPFLF 382
LS F SF AAA G A PPPPP + FLF
Sbjct: 411 SLSS---FTSSFFSSAAGAAAAAAPPPPPAGAATAAAAPPPPPAFKMRSSMFLF 259
>TC225213 UP|O64970 (O64970) Cationic peroxidase 2 , partial (64%)
Length = 859
Score = 30.0 bits (66), Expect = 2.0
Identities = 17/49 (34%), Positives = 26/49 (52%), Gaps = 7/49 (14%)
Frame = +1
Query: 224 ISSFDGFKVLGLPY-------KQGNDKRAFSIYFFLPDEKDGLSALIDK 265
+S+ DG LG P+ + G RA + FLPD + +SA++DK
Sbjct: 496 LSARDGIVSLGGPHIPLKTGRRDGRRSRADVVEQFLPDHNESISAVLDK 642
>CD392208 weakly similar to PIR|T02039|T020 acidic ribosomal protein P1a -
maize, partial (90%)
Length = 700
Score = 29.6 bits (65), Expect = 2.6
Identities = 21/58 (36%), Positives = 25/58 (42%)
Frame = +1
Query: 325 PISQELSVTSIFQKSFIELNEEGTIAAATARGSTGGAAPFRLPPPPPIDFVADHPFLF 382
P+S LS F SF + G AAA + G A PPPPP + FLF
Sbjct: 166 PMSSSLSS---FTSSFFS-SAAGAAAAAAPPPAAGAATAAAAPPPPPAFKMRSSMFLF 327
>BF008872
Length = 322
Score = 29.3 bits (64), Expect = 3.3
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = -1
Query: 362 APFRLPPPPPIDFVADHPFLFLIRE 386
A F +P P F+ +HPFLFL++E
Sbjct: 196 AAFPVPAPIAQPFLPNHPFLFLLKE 122
>TC224879 homologue to gb|AF223066.1|AF223066 Humulus lupulus 26S ribosomal
RNA gene, partial sequence; 18S ribosomal RNA gene,
internal transcribed spacer 1, 5.8S ribosomal RNA gene,
internal transcribed spacer 2 and 26S ribosomal RNA
gene, complete sequence; and 18S ribosomal RNA gene,
partial sequence, partial (5%)
Length = 1911
Score = 28.9 bits (63), Expect = 4.4
Identities = 20/59 (33%), Positives = 24/59 (39%)
Frame = +1
Query: 324 SPISQELSVTSIFQKSFIELNEEGTIAAATARGSTGGAAPFRLPPPPPIDFVADHPFLF 382
+PIS LS + SF + G AAA + GAA PPP F P F
Sbjct: 325 NPISSSLSSSG---SSFFFSSAAGAAAAAPPAAAATGAAATATGAPPPAAFAIKSPRFF 492
>TC223205
Length = 448
Score = 28.9 bits (63), Expect = 4.4
Identities = 16/45 (35%), Positives = 24/45 (52%)
Frame = +3
Query: 62 EGPTQNQLLSFLQSESTGDLKSLCSQLVSSVLSDGAPAGGPCLSH 106
+GPTQ ++ + S+ D K +CS V + + G GG C SH
Sbjct: 36 DGPTQQKIHAIDVSKEEVDGK-ICSDEVPATIGAGDLDGGDCFSH 167
>TC222372 similar to UP|Q7KS14 (Q7KS14) CG6695-PB, partial (5%)
Length = 465
Score = 28.5 bits (62), Expect = 5.7
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +1
Query: 349 IAAATARGSTGGAAPFRLPPPPPI 372
+A A G AP+RLPPPPP+
Sbjct: 49 LARAPFLGPVLPRAPYRLPPPPPL 120
>TC212974 weakly similar to UP|COM3_POPKI (Q43047) Caffeic acid
3-O-methyltransferase 3
(S-adenosysl-L-methionine:caffeic acid
3-O-methyltransferase 1) (COMT-3) (CAOMT-3) , partial
(32%)
Length = 841
Score = 28.1 bits (61), Expect = 7.4
Identities = 16/49 (32%), Positives = 25/49 (50%), Gaps = 8/49 (16%)
Frame = +2
Query: 189 WKRPF-----DTSK---TKDYDFDLLNGKSVKVPFMTSKNNQFISSFDG 229
W+ P+ TSK K +FDL +GK++ +PF S +F+ G
Sbjct: 668 WRGPWCHLNIGTSKYPYIKGLNFDLPHGKNMPLPFWVSMGREFLKGAQG 814
>TC204381 similar to UP|CRTC_RICCO (P93508) Calreticulin precursor, partial
(81%)
Length = 1408
Score = 28.1 bits (61), Expect = 7.4
Identities = 27/106 (25%), Positives = 50/106 (46%), Gaps = 1/106 (0%)
Frame = -3
Query: 4 QKRRTSEQNLIGKSLTNLTNVSLNITKHLLSNQKLNEKNVVFSPLSL-NTVLIMITAGSE 62
QK T+E+++ +++T+ ++S+ T + ++V SP S+ ++ + A S
Sbjct: 1124 QKNTTTEKDITFRNITSAHSLSVLQTS*---SSSCTPSSLVSSPASVFDSASESLPASSA 954
Query: 63 GPTQNQLLSFLQSESTGDLKSLCSQLVSSVLSDGAPAGGPCLSHVN 108
+ + SESTG S L S S+ A + CL HV+
Sbjct: 953 SSSSSSA-----SESTGSSFDSSSSLFFSASSNAAFSASLCLPHVS 831
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.136 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,780,894
Number of Sequences: 63676
Number of extensions: 205923
Number of successful extensions: 1617
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1572
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1600
length of query: 404
length of database: 12,639,632
effective HSP length: 99
effective length of query: 305
effective length of database: 6,335,708
effective search space: 1932390940
effective search space used: 1932390940
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC122166.8