BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122166.2 + phase: 0
(395 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC216434 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Frag... 483 e-137
TC216436 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Frag... 208 4e-54
TC216435 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Frag... 181 4e-46
TC226173 similar to UP|PS21_ARATH (O23714) Proteasome subunit be... 31 1.1
TC206592 30 1.5
TC216978 homologue to UP|Q8RXH5 (Q8RXH5) Osmotic stress-activate... 30 1.9
TC226174 similar to UP|PS21_ARATH (O23714) Proteasome subunit be... 30 1.9
TC220766 homologue to UP|Q9Q5L3 (Q9Q5L3) EBNA-2, partial (4%) 30 1.9
AW278727 similar to PIR|T06227|T06 peroxidase (EC 1.11.1.7) 2 c... 30 2.5
TC231230 weakly similar to GB|CAA65563.1|1403551|SCLACHXVI P2570... 29 3.2
TC222652 similar to UP|Q99491 (Q99491) DAN15 protein (Fragment),... 29 4.2
CD392995 GP|2245671|gb| e3B1 {Homo sapiens}, partial (3%) 29 4.2
TC226426 28 5.5
BI971672 28 5.5
TC225213 UP|O64970 (O64970) Cationic peroxidase 2 , partial (64%) 28 5.5
TC225212 UP|O64970 (O64970) Cationic peroxidase 2 , complete 28 5.5
TC214928 UP|Q9XFI6 (Q9XFI6) Peroxidase, complete 28 5.5
BE191314 28 5.5
TC220920 similar to UP|O65001 (O65001) Terminal ear1, partial (9%) 28 7.2
TC209045 28 7.2
>TC216434 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Fragment),
partial (96%)
Length = 1436
Score = 483 bits (1244), Expect = e-137
Identities = 242/386 (62%), Positives = 310/386 (79%)
Frame = +1
Query: 7 KSFTNLTNISMNITKHLLSNQKLKEENVVFSPLSLNTVLSMIATGSEGPTQKQLLSFLQS 66
+S +N T+++++I+K LLS + +++N+V+SPLSL+ VLS+IA GS+GPT QLLSFL+S
Sbjct: 22 ESISNQTDVALSISKLLLSKEA-RDKNLVYSPLSLHVVLSIIAAGSKGPTLDQLLSFLRS 198
Query: 67 ESTGDLKSLCSQLVSSVLSDGAPAGGPCLSYVNGVWVEQTIPLQPSFKQLMNTDFKAAFA 126
+ST L S SQL + VLSD +PAGGP LS+ +GVWVEQ++ L PSFKQL++ D+KA A
Sbjct: 199 KSTDHLNSFASQLFAVVLSDASPAGGPRLSFADGVWVEQSLSLLPSFKQLVSADYKATLA 378
Query: 127 AVDFVNKANEVGEEVNFWAEKETKGLIKNLLPPGSVNSLTRLIFANALYFKGVWKQQFDT 186
+VDF KA EV EVN WAEKET GL+K+LLPPGSV+S TRLIFANALYFKG W ++FD+
Sbjct: 379 SVDFQTKAVEVANEVNSWAEKETNGLVKDLLPPGSVDSSTRLIFANALYFKGAWNEKFDS 558
Query: 187 TKTKDYDFDLLNGKSVKVPFMTSKNDQFISSLDGFKVLGLPYKQGKDERAFSIYFFLPDK 246
+ TKDYDF LL+G+S++VPFMTS+ +QFI + DGFKVLGLPYKQG+D+R F++YFFLP+
Sbjct: 559 SITKDYDFHLLDGRSIRVPFMTSRKNQFIRAFDGFKVLGLPYKQGEDKRQFTMYFFLPET 738
Query: 247 KDGLSNLIDKVASDSEFLERNLPRRKVEVGKFRIPRFNISFEIEASELLKKLGLALPFTL 306
KDGL L +K+AS+S FLER LP K+EVG FRIPRF ISF EAS +LK+LG+ LPF++
Sbjct: 739 KDGLLALAEKLASESGFLERKLPNNKLEVGDFRIPRFKISFGFEASNVLKELGVVLPFSV 918
Query: 307 GGLTKMVDSPISQELYVSGIFQKSFIEVNEEGTKAAAVTVSFISSRSRYSPPPPPPIDFV 366
GGLT+MVDS + Q L+VS IF KSFIEVNEEGT+AAA T + I P IDFV
Sbjct: 919 GGLTEMVDSAVGQNLFVSDIFHKSFIEVNEEGTEAAAATAATIQFGCAMF---PTEIDFV 1089
Query: 367 ADHPFLFLIREEFSGTILFVGKVVNP 392
ADHPFLFLIRE+ +GT+LF+G+V+NP
Sbjct: 1090ADHPFLFLIREDLTGTVLFIGQVLNP 1167
>TC216436 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Fragment),
partial (40%)
Length = 489
Score = 208 bits (529), Expect = 4e-54
Identities = 103/161 (63%), Positives = 124/161 (76%)
Frame = +2
Query: 109 LQPSFKQLMNTDFKAAFAAVDFVNKANEVGEEVNFWAEKETKGLIKNLLPPGSVNSLTRL 168
L PSFKQL+ +KA A+VDF KA EV EVN WAEKET GL+K+LLPPGSV++ T+L
Sbjct: 5 LHPSFKQLVRAQYKATLASVDFQTKAVEVTNEVNSWAEKETNGLVKDLLPPGSVDNSTKL 184
Query: 169 IFANALYFKGVWKQQFDTTKTKDYDFDLLNGKSVKVPFMTSKNDQFISSLDGFKVLGLPY 228
IFANAL+ KG W ++FD + TK YD LLNG SVKVPFMTSK QFI + + FKVLGL Y
Sbjct: 185 IFANALWCKGAWNEKFDASITKHYDLHLLNGSSVKVPFMTSKKKQFIMA*NSFKVLGLSY 364
Query: 229 KQGKDERAFSIYFFLPDKKDGLSNLIDKVASDSEFLERNLP 269
KQG+D+R F++YFFLP+ KDGL L +K+A DS FLER LP
Sbjct: 365 KQGEDKRQFTMYFFLPETKDGLLALAEKLAFDSGFLERRLP 487
>TC216435 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Fragment),
partial (37%)
Length = 820
Score = 181 bits (460), Expect = 4e-46
Identities = 94/150 (62%), Positives = 115/150 (76%)
Frame = +2
Query: 246 KKDGLSNLIDKVASDSEFLERNLPRRKVEVGKFRIPRFNISFEIEASELLKKLGLALPFT 305
+ DGL L +K+AS+S FLER LP +KVEVG FRIPRF ISF E S +LK+LG+ LPF+
Sbjct: 5 RPDGLLALAEKLASESGFLERKLPNQKVEVGDFRIPRFKISFGFEVSNVLKELGVVLPFS 184
Query: 306 LGGLTKMVDSPISQELYVSGIFQKSFIEVNEEGTKAAAVTVSFISSRSRYSPPPPPPIDF 365
+GGLT+MVDSP+ Q L VS IF KSFIEVNEEGT+AAA T + I RS P IDF
Sbjct: 185 VGGLTEMVDSPVGQNLCVSNIFHKSFIEVNEEGTEAAAATSATIRLRSAML---PTKIDF 355
Query: 366 VADHPFLFLIREEFSGTILFVGKVVNPLDG 395
VADHPFLFLIRE+ +GT+LF+G+V++P G
Sbjct: 356 VADHPFLFLIREDLTGTVLFIGQVLDPRAG 445
>TC226173 similar to UP|PS21_ARATH (O23714) Proteasome subunit beta type 2-1
(20S proteasome alpha subunit D1) , partial (98%)
Length = 930
Score = 30.8 bits (68), Expect = 1.1
Identities = 12/17 (70%), Positives = 13/17 (75%)
Frame = +1
Query: 356 SPPPPPPIDFVADHPFL 372
SPPPPPPI VA+ P L
Sbjct: 298 SPPPPPPISHVANSPLL 348
>TC206592
Length = 1258
Score = 30.4 bits (67), Expect = 1.5
Identities = 19/47 (40%), Positives = 25/47 (52%)
Frame = +1
Query: 347 SFISSRSRYSPPPPPPIDFVADHPFLFLIREEFSGTILFVGKVVNPL 393
S I R PPPPPP + F FL R+E+ G I G+++ PL
Sbjct: 949 SCILPRFLEGPPPPPP---TTHNHFAFLKRDEYRG-IFVQGRLLFPL 1077
>TC216978 homologue to UP|Q8RXH5 (Q8RXH5) Osmotic stress-activated protein
kinase, partial (96%)
Length = 1626
Score = 30.0 bits (66), Expect = 1.9
Identities = 15/30 (50%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
Frame = -2
Query: 339 TKAAAVTVSFISSRSRYSPPPP-PPIDFVA 367
+ + + T SF+SS S SPP P PPID A
Sbjct: 1355 SSSVSATASFVSSSSSSSPPQPNPPIDLDA 1266
>TC226174 similar to UP|PS21_ARATH (O23714) Proteasome subunit beta type 2-1
(20S proteasome alpha subunit D1) , partial (85%)
Length = 752
Score = 30.0 bits (66), Expect = 1.9
Identities = 12/17 (70%), Positives = 13/17 (75%)
Frame = +2
Query: 356 SPPPPPPIDFVADHPFL 372
SPPPPPPI VA+ P L
Sbjct: 152 SPPPPPPISHVANLPLL 202
>TC220766 homologue to UP|Q9Q5L3 (Q9Q5L3) EBNA-2, partial (4%)
Length = 885
Score = 30.0 bits (66), Expect = 1.9
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +1
Query: 354 RYSPPPPPPIDFVADHPFLFLI 375
R SPPPPPP+ ++ P L L+
Sbjct: 166 RASPPPPPPLSTISYKPLLLLL 231
Score = 25.0 bits (53), Expect(2) = 4.8
Identities = 9/24 (37%), Positives = 14/24 (57%)
Frame = +3
Query: 339 TKAAAVTVSFISSRSRYSPPPPPP 362
T+ T + + + + SPPPPPP
Sbjct: 165 TRFTTTTTTTLHHQLQASPPPPPP 236
Score = 21.9 bits (45), Expect(2) = 4.8
Identities = 6/8 (75%), Positives = 8/8 (100%)
Frame = +3
Query: 357 PPPPPPID 364
PPPPPP++
Sbjct: 222 PPPPPPLN 245
>AW278727 similar to PIR|T06227|T06 peroxidase (EC 1.11.1.7) 2 cationic -
soybean, partial (55%)
Length = 556
Score = 29.6 bits (65), Expect = 2.5
Identities = 16/49 (32%), Positives = 26/49 (52%), Gaps = 7/49 (14%)
Frame = +2
Query: 215 ISSLDGFKVLGLPY-------KQGKDERAFSIYFFLPDKKDGLSNLIDK 256
+S++DG LG P+ G+ RA + FLPD + +S ++DK
Sbjct: 383 LSAIDGIDTLGGPHIPLKTXRMDGRMSRADVVEQFLPDHNESISAVLDK 529
>TC231230 weakly similar to GB|CAA65563.1|1403551|SCLACHXVI P2570 protein
{Saccharomyces cerevisiae;} , partial (3%)
Length = 842
Score = 29.3 bits (64), Expect = 3.2
Identities = 34/120 (28%), Positives = 50/120 (41%), Gaps = 23/120 (19%)
Frame = +2
Query: 266 RNLPRRKVEVGKFRIPRFNISFEIEASELLKKLGLALPFTL-------------GGLTKM 312
RNLPRRK KF P + E ++K G+++P + L K
Sbjct: 173 RNLPRRK----KFPPPVYGKHVE-HLKSVIKACGMSVPPVIYKKVKQVPENKREEQLIKE 337
Query: 313 VDSPISQELYVSGIFQKSFIEVNEEGTKAA---AVTVSFISSRSRY-------SPPPPPP 362
++ +S+E S +K EV + +A + +S I S SR SPPPP P
Sbjct: 338 LEEILSREGLSSNPSEKEIKEVKRKKARAKELEGIDLSNIVSSSRRRSTSSYTSPPPPKP 517
>TC222652 similar to UP|Q99491 (Q99491) DAN15 protein (Fragment), partial
(8%)
Length = 424
Score = 28.9 bits (63), Expect = 4.2
Identities = 16/42 (38%), Positives = 24/42 (57%), Gaps = 3/42 (7%)
Frame = -3
Query: 349 ISSRSRYSPPPPPP-IDFVADHPFLF--LIREEFSGTILFVG 387
I++ +R+ PPPPP + F PF+F + + S IL VG
Sbjct: 353 IAATNRHRRPPPPPLLPFHVSGPFVFPXSVSDNISWLILLVG 228
>CD392995 GP|2245671|gb| e3B1 {Homo sapiens}, partial (3%)
Length = 275
Score = 28.9 bits (63), Expect = 4.2
Identities = 15/37 (40%), Positives = 19/37 (50%)
Frame = -2
Query: 343 AVTVSFISSRSRYSPPPPPPIDFVADHPFLFLIREEF 379
A V FI + + PPPPPP F+ F F I +F
Sbjct: 148 AYFVLFILAANLDRPPPPPPHAFLVISSFFF*ITTQF 38
>TC226426
Length = 1158
Score = 28.5 bits (62), Expect = 5.5
Identities = 21/63 (33%), Positives = 30/63 (47%), Gaps = 1/63 (1%)
Frame = +1
Query: 325 GIFQKSFIEVNEEGTKAAAVTVSFISSRSRYSPPPPPPIDFVADHPFLFLIRE-EFSGTI 383
G+F FI + E SFISS +R+SP P P + P+L +I S +
Sbjct: 136 GVFSSDFIWICER*R-------SFISSIARFSPSAPTP-STILPRPYLSMIHSAPLSKPL 291
Query: 384 LFV 386
LF+
Sbjct: 292 LFI 300
>BI971672
Length = 354
Score = 28.5 bits (62), Expect = 5.5
Identities = 11/16 (68%), Positives = 12/16 (74%)
Frame = +2
Query: 357 PPPPPPIDFVADHPFL 372
PPPPPPI VA+ P L
Sbjct: 236 PPPPPPISRVANSPLL 283
>TC225213 UP|O64970 (O64970) Cationic peroxidase 2 , partial (64%)
Length = 859
Score = 28.5 bits (62), Expect = 5.5
Identities = 16/49 (32%), Positives = 26/49 (52%), Gaps = 7/49 (14%)
Frame = +1
Query: 215 ISSLDGFKVLGLPY-------KQGKDERAFSIYFFLPDKKDGLSNLIDK 256
+S+ DG LG P+ + G+ RA + FLPD + +S ++DK
Sbjct: 496 LSARDGIVSLGGPHIPLKTGRRDGRRSRADVVEQFLPDHNESISAVLDK 642
>TC225212 UP|O64970 (O64970) Cationic peroxidase 2 , complete
Length = 1484
Score = 28.5 bits (62), Expect = 5.5
Identities = 16/49 (32%), Positives = 26/49 (52%), Gaps = 7/49 (14%)
Frame = +1
Query: 215 ISSLDGFKVLGLPY-------KQGKDERAFSIYFFLPDKKDGLSNLIDK 256
+S+ DG LG P+ + G+ RA + FLPD + +S ++DK
Sbjct: 406 LSARDGIVSLGGPHIPLKTGRRDGRRSRADVVEQFLPDHNESISAVLDK 552
>TC214928 UP|Q9XFI6 (Q9XFI6) Peroxidase, complete
Length = 1545
Score = 28.5 bits (62), Expect = 5.5
Identities = 16/49 (32%), Positives = 26/49 (52%), Gaps = 7/49 (14%)
Frame = +2
Query: 215 ISSLDGFKVLGLPY-------KQGKDERAFSIYFFLPDKKDGLSNLIDK 256
+S+ DG LG P+ + G+ RA + FLPD + +S ++DK
Sbjct: 506 LSARDGIVSLGGPHIPLKTGRRDGRRSRADVVEQFLPDHNESISAVLDK 652
>BE191314
Length = 473
Score = 28.5 bits (62), Expect = 5.5
Identities = 16/49 (32%), Positives = 26/49 (52%), Gaps = 7/49 (14%)
Frame = +2
Query: 215 ISSLDGFKVLGLPY-------KQGKDERAFSIYFFLPDKKDGLSNLIDK 256
+S+ DG LG P+ + G+ RA + FLPD + +S ++DK
Sbjct: 323 LSARDGIVSLGGPHIPLKTGRRDGRRSRADVVEQFLPDHNESISAVLDK 469
>TC220920 similar to UP|O65001 (O65001) Terminal ear1, partial (9%)
Length = 577
Score = 28.1 bits (61), Expect = 7.2
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = -2
Query: 345 TVSFISSRSRYSPPPPPPID 364
T SF+SS S P PPPP+D
Sbjct: 267 TSSFVSSSSPTLPLPPPPLD 208
>TC209045
Length = 1104
Score = 28.1 bits (61), Expect = 7.2
Identities = 23/86 (26%), Positives = 39/86 (44%)
Frame = -1
Query: 126 AAVDFVNKANEVGEEVNFWAEKETKGLIKNLLPPGSVNSLTRLIFANALYFKGVWKQQFD 185
+A FVN+ E + F + T+ + KNLLPP S + A+ LYF ++
Sbjct: 708 SAQHFVNEDKE*YQHR*FQHKLRTRRVFKNLLPPISF----KFRVASLLYFLLCKFFKYS 541
Query: 186 TTKTKDYDFDLLNGKSVKVPFMTSKN 211
T D++ + + +P M K+
Sbjct: 540 TLSFIDFNRKINTESYILIPMMYKKS 463
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.136 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,699,503
Number of Sequences: 63676
Number of extensions: 221087
Number of successful extensions: 4275
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 2618
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3909
length of query: 395
length of database: 12,639,632
effective HSP length: 99
effective length of query: 296
effective length of database: 6,335,708
effective search space: 1875369568
effective search space used: 1875369568
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC122166.2