BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122165.8 - phase: 1 /pseudo
(144 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC208191 similar to GB|AAP21345.1|30102854|BT006537 At5g55640 {A... 189 4e-49
TC227282 homologue to GB|AAD14482.1|4249385|T2K10 T2K10.11 {Arab... 32 0.10
TC233988 similar to UP|Q9ZTU9 (Q9ZTU9) Protein kinase CPK1, part... 32 0.14
TC219573 30 0.40
TC209198 homologue to GB|BAB62912.1|15207792|AB055883 ERF domain... 29 0.68
CF921661 29 0.89
TC225135 similar to UP|Q9M549 (Q9M549) Poly(A)-binding protein, ... 29 0.89
TC226599 28 1.2
TC232400 weakly similar to UP|Q8W2K5 (Q8W2K5) Phragmoplastin-int... 28 1.2
CK605876 27 2.6
CA852082 27 3.4
TC208985 UP|Q6PV95 (Q6PV95) Beta-carotene hydroxylase, partial (... 27 3.4
TC209197 similar to GB|BAB62912.1|15207792|AB055883 ERF domain p... 27 3.4
TC207061 similar to UP|Q8RXD0 (Q8RXD0) Auxilin-like protein (At4... 26 5.8
TC231391 UP|Q39846 (Q39846) LEA protein, complete 26 5.8
TC231066 homologue to GB|AAM52878.1|21539884|AY102636 transcript... 26 5.8
TC216626 similar to UP|VIL2_ARATH (O81644) Villin 2, partial (36%) 26 7.5
TC227283 homologue to GB|AAD14482.1|4249385|T2K10 T2K10.11 {Arab... 26 7.5
TC220932 similar to GB|AAO42883.1|28416705|BT004637 At5g11030 {A... 26 7.5
CF921606 26 7.5
>TC208191 similar to GB|AAP21345.1|30102854|BT006537 At5g55640 {Arabidopsis
thaliana;} , partial (62%)
Length = 1006
Score = 189 bits (480), Expect = 4e-49
Identities = 95/123 (77%), Positives = 103/123 (83%)
Frame = +3
Query: 22 DTDGFVIPSLGIQDSDQSKNNAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPS 81
DTDGFVIPSLGI+DSDQ+K + VES NSAA+ KKEE IYLGPHGAPPS KQQE+ S
Sbjct: 249 DTDGFVIPSLGIEDSDQNKPHVHDVESSNSAAQAKKEENIYLGPHGAPPSLAKQQELNSS 428
Query: 82 NRKQRFKQKLKEADKRISGTGRENKVDNLRELVGGEKTSVGMAKGSSPKDWLDPHCHEAE 141
NRKQ+FKQKLKEADKRISGTGRENKVDNLRELVGG K + AK S KDWLDPHC E+E
Sbjct: 429 NRKQKFKQKLKEADKRISGTGRENKVDNLRELVGGGKLCINNAK-ESTKDWLDPHCQESE 605
Query: 142 FER 144
FER
Sbjct: 606 FER 614
>TC227282 homologue to GB|AAD14482.1|4249385|T2K10 T2K10.11 {Arabidopsis
thaliana;} , partial (61%)
Length = 1408
Score = 32.0 bits (71), Expect = 0.10
Identities = 21/80 (26%), Positives = 38/80 (47%)
Frame = +2
Query: 65 PHGAPPSQPKQQEVIPSNRKQRFKQKLKEADKRISGTGRENKVDNLRELVGGEKTSVGMA 124
P PP+ +QQ ++PSN +Q+ ++ L + I G + K + +E V + +G +
Sbjct: 941 PSVVPPTTTQQQPLLPSNSQQQQRRPL----EFIMGMQKVAKEELEQEEVYRPELDIGES 1108
Query: 125 KGSSPKDWLDPHCHEAEFER 144
S P H H+ F +
Sbjct: 1109 SSSLPGGGYHQHQHQHHFHQ 1168
>TC233988 similar to UP|Q9ZTU9 (Q9ZTU9) Protein kinase CPK1, partial (11%)
Length = 620
Score = 31.6 bits (70), Expect = 0.14
Identities = 16/49 (32%), Positives = 26/49 (52%)
Frame = +1
Query: 45 SVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSNRKQRFKQKLKE 93
S S N+AA + + K P APP+QP+ + V +KQ + K ++
Sbjct: 85 SGSSSNNAAASNRNRKGLAAPAPAPPAQPELEPVTGKQKKQPSQAKRRQ 231
>TC219573
Length = 872
Score = 30.0 bits (66), Expect = 0.40
Identities = 16/58 (27%), Positives = 34/58 (58%), Gaps = 3/58 (5%)
Frame = +3
Query: 65 PHGAPPSQPKQQEVIPSNRKQRFKQKLKEADKR---ISGTGRENKVDNLRELVGGEKT 119
P+G PP+ P++Q+ S Q+ K K + A +R + RE++ + +R+++G + +
Sbjct: 75 PNGLPPAPPRKQKEKLSEVDQQLK-KAEAAQRRRMQVEKAARESEAEAIRKILGQDSS 245
>TC209198 homologue to GB|BAB62912.1|15207792|AB055883 ERF domain protein12
{Arabidopsis thaliana;} , partial (42%)
Length = 637
Score = 29.3 bits (64), Expect = 0.68
Identities = 16/42 (38%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
Frame = +3
Query: 49 PNSAAKTKKEEKIYLGPHGAP--PSQPKQQEVIPSNRKQRFK 88
P+ AA + ++ +L AP PSQP+ Q V P R QR +
Sbjct: 366 PSGAASLPRPQRPFLRSPLAPRPPSQPRPQRVPPHRRSQRHR 491
>CF921661
Length = 293
Score = 28.9 bits (63), Expect = 0.89
Identities = 18/45 (40%), Positives = 22/45 (48%)
Frame = +2
Query: 49 PNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSNRKQRFKQKLKE 93
PNS K KK+ PHG PP PK E P K+ +K K+
Sbjct: 86 PNSGEKGKKKGTPRY-PHGNPPPPPKGMEKPPPQGKKTPGKKKKK 217
>TC225135 similar to UP|Q9M549 (Q9M549) Poly(A)-binding protein, partial
(97%)
Length = 2392
Score = 28.9 bits (63), Expect = 0.89
Identities = 25/87 (28%), Positives = 43/87 (48%)
Frame = +3
Query: 32 GIQDSDQSKNNAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSNRKQRFKQKL 91
G + + + + A +VE+ N K +++ Y+G A ++ E+ KQRF+Q +
Sbjct: 846 GFVNFENADDAARAVEALNG--KKFDDKEWYVGK--AQKKSERENEL-----KQRFEQSM 998
Query: 92 KEADKRISGTGRENKVDNLRELVGGEK 118
KEA + G V NL + +G EK
Sbjct: 999 KEAADKYQGANL--YVKNLDDSIGDEK 1073
>TC226599
Length = 937
Score = 28.5 bits (62), Expect = 1.2
Identities = 18/54 (33%), Positives = 23/54 (42%)
Frame = -3
Query: 29 PSLGIQDSDQSKNNAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSN 82
P+ GI D SK SV +S TK Y +PP+ P +PSN
Sbjct: 434 PTTGIPDDMASKTTRPSVS--DSXXITKTSPLAYASDSSSPPNIPVNTAGMPSN 279
>TC232400 weakly similar to UP|Q8W2K5 (Q8W2K5) Phragmoplastin-interacting
protein PHIP1, partial (8%)
Length = 621
Score = 28.5 bits (62), Expect = 1.2
Identities = 25/85 (29%), Positives = 39/85 (45%), Gaps = 1/85 (1%)
Frame = +2
Query: 48 SPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSNRKQRFKQKL-KEADKRISGTGRENK 106
SPN K KK E + +G + P N+K++ KQK K +K+ S T EN+
Sbjct: 233 SPNKKRKRKKVEGDDVAANGVSDT--------PKNKKKKQKQKKNKNKNKKKSTTAEENE 388
Query: 107 VDNLRELVGGEKTSVGMAKGSSPKD 131
+ E+ E T + ++ KD
Sbjct: 389 SNGGGEM--PEATELTKTNTTTSKD 457
>CK605876
Length = 565
Score = 27.3 bits (59), Expect = 2.6
Identities = 24/81 (29%), Positives = 35/81 (42%), Gaps = 7/81 (8%)
Frame = +1
Query: 27 VIPSLGIQDSDQSKN-NAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVI------ 79
VIP LGI+ N E+P S TKK E +L GAPP++ V+
Sbjct: 190 VIPRLGIKTESPGLNIPRGKFENPPS---TKKGELPHLLQKGAPPNESDGAAVVLPLGQG 360
Query: 80 PSNRKQRFKQKLKEADKRISG 100
Q+ + +L+ K+ G
Sbjct: 361 EVENPQKIQLRLRRGGKKALG 423
>CA852082
Length = 863
Score = 26.9 bits (58), Expect = 3.4
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = -3
Query: 49 PNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSNRKQRFK 88
PN K K + P +PPSQPKQ+ + R +F+
Sbjct: 858 PNLERKGKSSNPS-IEPRASPPSQPKQKNLGNKGRLSQFR 742
>TC208985 UP|Q6PV95 (Q6PV95) Beta-carotene hydroxylase, partial (95%)
Length = 967
Score = 26.9 bits (58), Expect = 3.4
Identities = 16/46 (34%), Positives = 24/46 (51%)
Frame = -2
Query: 92 KEADKRISGTGRENKVDNLRELVGGEKTSVGMAKGSSPKDWLDPHC 137
+++D+R S REN V+ R L V + +GS PK + P C
Sbjct: 648 EKSDRRDSVNYRENVVELERSLSWSMV*LVHVPQGSMPKSSMSPSC 511
>TC209197 similar to GB|BAB62912.1|15207792|AB055883 ERF domain protein12
{Arabidopsis thaliana;} , partial (17%)
Length = 529
Score = 26.9 bits (58), Expect = 3.4
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = +2
Query: 69 PPSQPKQQEVIPSNRKQR 86
PPSQP+ Q V P R QR
Sbjct: 113 PPSQPRPQRVPPHRRSQR 166
>TC207061 similar to UP|Q8RXD0 (Q8RXD0) Auxilin-like protein (At4g12780),
partial (42%)
Length = 1760
Score = 26.2 bits (56), Expect = 5.8
Identities = 10/19 (52%), Positives = 12/19 (62%)
Frame = +3
Query: 122 GMAKGSSPKDWLDPHCHEA 140
G +GSS D LDPHC +
Sbjct: 840 GGRQGSSSSDMLDPHCQNS 896
>TC231391 UP|Q39846 (Q39846) LEA protein, complete
Length = 2208
Score = 26.2 bits (56), Expect = 5.8
Identities = 21/69 (30%), Positives = 28/69 (40%), Gaps = 6/69 (8%)
Frame = +1
Query: 40 KNNAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEVIPSN------RKQRFKQKLKE 93
K AAS KK+E+ PSQP++ E PS R+ K K E
Sbjct: 1297 KGLAASAGETAKEYTAKKKEEAQRELEAKKPSQPQEAEERPSEGIGETVRQYAQKPKPSE 1476
Query: 94 ADKRISGTG 102
+ + GTG
Sbjct: 1477 RNPQKEGTG 1503
>TC231066 homologue to GB|AAM52878.1|21539884|AY102636 transcription
activator {Arabidopsis thaliana;} , partial (17%)
Length = 793
Score = 26.2 bits (56), Expect = 5.8
Identities = 15/50 (30%), Positives = 23/50 (46%), Gaps = 4/50 (8%)
Frame = +1
Query: 33 IQDSDQSKNNAA----SVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQEV 78
+QDSD +NNAA S+ P +AA + + L P+ + V
Sbjct: 289 LQDSDNVENNAAGPSLSISMPGNAAAASSDVSLKLSTGYGEDPGPRNENV 438
>TC216626 similar to UP|VIL2_ARATH (O81644) Villin 2, partial (36%)
Length = 1777
Score = 25.8 bits (55), Expect = 7.5
Identities = 12/32 (37%), Positives = 17/32 (52%)
Frame = -1
Query: 39 SKNNAASVESPNSAAKTKKEEKIYLGPHGAPP 70
+K +A S + PN+ K K + LGP G P
Sbjct: 718 AKPDAFSEDEPNALDKAAKASALCLGPPGLDP 623
>TC227283 homologue to GB|AAD14482.1|4249385|T2K10 T2K10.11 {Arabidopsis
thaliana;} , partial (27%)
Length = 1192
Score = 25.8 bits (55), Expect = 7.5
Identities = 12/34 (35%), Positives = 17/34 (49%)
Frame = +2
Query: 65 PHGAPPSQPKQQEVIPSNRKQRFKQKLKEADKRI 98
P PPS +QQ ++PS R F K A + +
Sbjct: 443 PSVVPPSTQQQQSLLPSQRPLEFMGMQKVAKEEL 544
>TC220932 similar to GB|AAO42883.1|28416705|BT004637 At5g11030 {Arabidopsis
thaliana;} , partial (15%)
Length = 876
Score = 25.8 bits (55), Expect = 7.5
Identities = 21/77 (27%), Positives = 35/77 (45%), Gaps = 2/77 (2%)
Frame = +1
Query: 33 IQDSDQSKNNAASVESPNSAAKTKKEEKIYLGPHGAPPSQPKQQE-VIPSNRKQRFKQKL 91
++D+ Q N A S + + E + P G PPS P+Q + V+ + RF
Sbjct: 331 VKDAPQIDNKAFPDTSFWNPGILELVELVLRPPQGGPPSLPEQSDAVLSALNLYRFVLMT 510
Query: 92 KEADK-RISGTGRENKV 107
+ A+K I+G N +
Sbjct: 511 ESAEKTNITGVLSRNNL 561
>CF921606
Length = 518
Score = 25.8 bits (55), Expect = 7.5
Identities = 11/32 (34%), Positives = 16/32 (49%)
Frame = +2
Query: 65 PHGAPPSQPKQQEVIPSNRKQRFKQKLKEADK 96
P G PP PK++ P +K+ +K K K
Sbjct: 197 PRGKPPPPPKKKGPPPERKKKNGPKKKKNPQK 292
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.137 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,188,103
Number of Sequences: 63676
Number of extensions: 64735
Number of successful extensions: 506
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 505
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 505
length of query: 144
length of database: 12,639,632
effective HSP length: 88
effective length of query: 56
effective length of database: 7,036,144
effective search space: 394024064
effective search space used: 394024064
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC122165.8