BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122163.6 + phase: 0
(459 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC209002 similar to PIR|T01449|T01449 cytoskeletal protein homol... 308 3e-84
BQ081711 110 2e-24
TC230192 similar to UP|Q6Z2Q5 (Q6Z2Q5) Tetratricopeptide repeat ... 95 7e-20
TC209510 weakly similar to UP|Q6Z2Q5 (Q6Z2Q5) Tetratricopeptide ... 78 9e-15
BG043260 59 3e-09
AW101576 57 2e-08
BI699319 39 0.005
AW704183 37 0.024
TC219639 similar to UP|Q6K378 (Q6K378) Tetratricopeptide repeat ... 30 2.9
TC208696 similar to UP|Q93WT7 (Q93WT7) Hexose transporter pGlT, ... 30 2.9
BE330030 similar to GP|16648766|gb AT5g47860/MCA23_20 {Arabidops... 29 3.8
TC209506 28 6.5
TC207193 28 6.5
TC214344 weakly similar to GB|AAO11574.1|27363310|BT002658 At4g2... 28 8.6
TC231485 similar to GB|AAN31116.1|23506209|AY149962 At3g06130/F2... 28 8.6
TC228673 similar to UP|Q8L8M8 (Q8L8M8) Serine-rich protein, part... 28 8.6
TC229677 similar to UP|Q8VXD3 (Q8VXD3) N-acetylglucosaminyltrans... 28 8.6
TC221537 similar to UP|Q7XJJ2 (Q7XJJ2) Phosphoethanolamine N-met... 28 8.6
>TC209002 similar to PIR|T01449|T01449 cytoskeletal protein homolog F24O1.11
- Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(35%)
Length = 1139
Score = 308 bits (789), Expect = 3e-84
Identities = 166/295 (56%), Positives = 204/295 (68%), Gaps = 49/295 (16%)
Frame = +3
Query: 211 DKYIDFHDLGTEFCSDALEETVTSDEAQDLLDKAEFKFQEVAALAFFNWGNVHMCAARKF 270
D ++D H+LG E CS+ALEETVTS+EAQDL DKA KFQEVAALAFFNWGNVHMCAARK
Sbjct: 6 DAHLDLHELGMELCSEALEETVTSEEAQDLFDKAASKFQEVAALAFFNWGNVHMCAARKR 185
Query: 271 VRMDENENEVLVMNESE--FDFVQEKYYLAREKYEQAVVIKPDFYEGLLAIGQQQFELAK 328
+ +DE+ + +V + + +++V+EKY LAREKYE+A+ IKPDFYEGLLA+GQQQFE+AK
Sbjct: 186 IPLDESAGKEVVAEQLQVAYEWVKEKYSLAREKYEEALSIKPDFYEGLLALGQQQFEMAK 365
Query: 329 LNWSFGIANKMDL----GKETLRLFDVAEEKMTAANDAWENLEKGKL------------- 371
L+WSF +A K+DL KETL+LFD AEEKM AA D WE LE+ +
Sbjct: 366 LHWSFALAKKIDLSGWDSKETLQLFDSAEEKMKAATDMWEKLEEQRAKELKDPNATKKEE 545
Query: 372 ------------GEQGSVG----------------MRSQIHLFWGNMLFERSQVEFKLGM 403
GE SVG MRSQIHLFWGNMLFERSQVE KLGM
Sbjct: 546 LLRRRKKQGATEGESSSVGGQGEISAEEAAEQAAVMRSQIHLFWGNMLFERSQVECKLGM 725
Query: 404 SDWKKKLDASVERFKIAGASEADVSGILKKHCFNGNAR--DEKIKGSPRTHNVIK 456
+ WK+ LDA+ ERFK+AGASEADVS +LK HC NG+A+ D+K +P+ + +K
Sbjct: 726 TGWKENLDAATERFKLAGASEADVSMVLKNHCSNGDAKDGDDKKVENPQHNKTVK 890
>BQ081711
Length = 424
Score = 110 bits (274), Expect = 2e-24
Identities = 57/130 (43%), Positives = 82/130 (62%), Gaps = 2/130 (1%)
Frame = +1
Query: 214 IDFHDLGTEFCSDALEETVTSDEAQDLLDKAEFKFQEVAALAFFNWGNVHMCAARK--FV 271
+D H+ + +A+E++V S++AQ+L A KFQE+AALA FNWG+V M AR F
Sbjct: 1 LDTHEYAMKLYEEAIEDSVASNDAQELFRMAADKFQEMAALALFNWGSVQMSLARNQGFF 180
Query: 272 RMDENENEVLVMNESEFDFVQEKYYLAREKYEQAVVIKPDFYEGLLAIGQQQFELAKLNW 331
D L ++ ++ Q++Y A +YE+A+ IKPDFYEG LA+G QQFE A+L W
Sbjct: 181 LEDGARESSLEHIKAAYELAQKEYEKAEMRYEEALKIKPDFYEGYLALGHQQFEQARLCW 360
Query: 332 SFGIANKMDL 341
+ +A K DL
Sbjct: 361 CYAMACKKDL 390
>TC230192 similar to UP|Q6Z2Q5 (Q6Z2Q5) Tetratricopeptide repeat
protein-like, partial (15%)
Length = 909
Score = 94.7 bits (234), Expect = 7e-20
Identities = 57/151 (37%), Positives = 74/151 (48%), Gaps = 33/151 (21%)
Frame = +1
Query: 325 ELAKLNWSFGIANKMDL----GKETLRLFDVAEEKMTAANDAWENLEKGKLGEQGSVG-- 378
E AKL+W + +DL E L L++ AEE M WE EK L + S
Sbjct: 1 EQAKLSWYHALGCNVDLLTWPSTEVLHLYNNAEENMEKGMQIWEESEKQNLSKTSSSNDV 180
Query: 379 ---------------------------MRSQIHLFWGNMLFERSQVEFKLGMSDWKKKLD 411
MRSQI+L WG ML+ERS VEFKLG+ W + L+
Sbjct: 181 RLHLQNMGLDGLFKNISLDEFAAQEAHMRSQINLLWGTMLYERSFVEFKLGLPIWHESLE 360
Query: 412 ASVERFKIAGASEADVSGILKKHCFNGNARD 442
+VE+F++AGAS D++ ILK HC N A D
Sbjct: 361 VAVEKFELAGASPTDIAVILKNHCSNNTAVD 453
>TC209510 weakly similar to UP|Q6Z2Q5 (Q6Z2Q5) Tetratricopeptide repeat
protein-like, partial (17%)
Length = 984
Score = 77.8 bits (190), Expect = 9e-15
Identities = 33/62 (53%), Positives = 47/62 (75%)
Frame = +2
Query: 379 MRSQIHLFWGNMLFERSQVEFKLGMSDWKKKLDASVERFKIAGASEADVSGILKKHCFNG 438
MRSQIHL WG +L+ERS VE+KLG+ W++ L+ +VE+F++AG S D++ I+K HC N
Sbjct: 146 MRSQIHLLWGTLLYERSVVEYKLGLPTWEECLEVAVEKFELAGTSATDIAFIVKNHCSNE 325
Query: 439 NA 440
A
Sbjct: 326 TA 331
>BG043260
Length = 265
Score = 59.3 bits (142), Expect = 3e-09
Identities = 25/53 (47%), Positives = 36/53 (67%)
Frame = +2
Query: 385 LFWGNMLFERSQVEFKLGMSDWKKKLDASVERFKIAGASEADVSGILKKHCFN 437
L WG ML+E S VEFKLG+ W + + + E+F++AG S D++ +LK HC N
Sbjct: 2 LLWGTMLYELSIVEFKLGLPVWHESREDAAEKFELAGTSATDIAVMLKNHCSN 160
>AW101576
Length = 272
Score = 56.6 bits (135), Expect = 2e-08
Identities = 28/39 (71%), Positives = 31/39 (78%)
Frame = +3
Query: 373 EQGSVGMRSQIHLFWGNMLFERSQVEFKLGMSDWKKKLD 411
EQ +V MRS IHLFW NMLFERSQVE KL M+ WK+ LD
Sbjct: 159 EQAAV-MRSXIHLFWXNMLFERSQVECKLXMTGWKENLD 272
>BI699319
Length = 370
Score = 38.9 bits (89), Expect = 0.005
Identities = 20/41 (48%), Positives = 27/41 (65%)
Frame = -2
Query: 419 IAGASEADVSGILKKHCFNGNARDEKIKGSPRTHNVIKTMK 459
+AGASEAD+S +LK +C NG+AR+ R HN I +K
Sbjct: 345 LAGASEADISMVLKNNCANGDARE-----GSRGHNSITLVK 238
>AW704183
Length = 442
Score = 36.6 bits (83), Expect = 0.024
Identities = 20/63 (31%), Positives = 35/63 (54%), Gaps = 4/63 (6%)
Frame = +2
Query: 294 KYYLAREKYEQAVVIKPDFYEGLLAIGQQQFELAKLNWSFGIANKMDL----GKETLRLF 349
+Y A +Y++ + I+P+FYEG L + QQ + KL + + K DL + L+L+
Sbjct: 2 EYKKAEMRYKEPLKIRPNFYEGYLTLEHQQLKQPKLC*YYAMTYKKDLEANCSNKVLKLY 181
Query: 350 DVA 352
+ A
Sbjct: 182 NKA 190
>TC219639 similar to UP|Q6K378 (Q6K378) Tetratricopeptide repeat
protein-like, partial (4%)
Length = 723
Score = 29.6 bits (65), Expect = 2.9
Identities = 11/21 (52%), Positives = 16/21 (75%)
Frame = +3
Query: 417 FKIAGASEADVSGILKKHCFN 437
F++AGAS D++ +LK HC N
Sbjct: 3 FELAGASATDIAVMLKNHCXN 65
>TC208696 similar to UP|Q93WT7 (Q93WT7) Hexose transporter pGlT, partial
(23%)
Length = 1373
Score = 29.6 bits (65), Expect = 2.9
Identities = 11/41 (26%), Positives = 24/41 (57%), Gaps = 4/41 (9%)
Frame = -1
Query: 30 PSPKKSDQ----ISRRLREPKLGPTNEHLKENQKEDVKIAW 66
P+P++ D+ + R R P+ G H+K+ ++ +++ AW
Sbjct: 764 PAPREKDRT*RTVPRTARVPEYGARTFHVKDRERSNIEAAW 642
>BE330030 similar to GP|16648766|gb AT5g47860/MCA23_20 {Arabidopsis
thaliana}, partial (34%)
Length = 453
Score = 29.3 bits (64), Expect = 3.8
Identities = 19/53 (35%), Positives = 32/53 (59%), Gaps = 1/53 (1%)
Frame = +1
Query: 92 RDIVKEKFPISRSVLIKYKDNDDDLVTITST-EELRFAESCVYKTDSVEILKL 143
R ++K + ISR++LIK++ DDL+ TST ++ +ES + + I KL
Sbjct: 205 RRLIKSYYGISRNLLIKFQ---DDLIDETSTLAQVLSSESAISSVLDMSIRKL 354
>TC209506
Length = 1105
Score = 28.5 bits (62), Expect = 6.5
Identities = 25/87 (28%), Positives = 43/87 (48%), Gaps = 5/87 (5%)
Frame = +1
Query: 114 DDLVTITSTEELRFAESCVYKTDSVEILKLYIVEVSPEHEPPLLKEEKEEENNEKQ-KPL 172
D+L + ST+E + + K+ S E ++ E +PP ++EE E+EN K+ +
Sbjct: 136 DELEGVLSTKEEQDSSLPQQKSSSSEFHWDFMEW--EEFQPPNIEEEGEDENEGKENRRS 309
Query: 173 DCVLDEK----MCTECNKVVENLEIDD 195
C+ DE+ N +V E+DD
Sbjct: 310 KCLFDEEEQEMKIENNNNIVGFWEMDD 390
>TC207193
Length = 484
Score = 28.5 bits (62), Expect = 6.5
Identities = 19/55 (34%), Positives = 28/55 (50%), Gaps = 9/55 (16%)
Frame = +1
Query: 136 DSVEILKLYIV----EVSPEHEPPLLK-----EEKEEENNEKQKPLDCVLDEKMC 181
D +I K+YIV +P E +K EEK+EE +K++ +LD K C
Sbjct: 31 DINQINKIYIVLGKNNTNPAPEKKKIKKKIKMEEKQEEERKKEREKGKILDVKTC 195
>TC214344 weakly similar to GB|AAO11574.1|27363310|BT002658
At4g27380/M4I22_190 {Arabidopsis thaliana;} , partial
(17%)
Length = 457
Score = 28.1 bits (61), Expect = 8.6
Identities = 13/36 (36%), Positives = 17/36 (47%)
Frame = +1
Query: 353 EEKMTAANDAWENLEKGKLGEQGSVGMRSQIHLFWG 388
EEK N + + G G +G+RS H FWG
Sbjct: 94 EEKRCRFNGYY*RIRFGTTSSNGYIGVRSSKHGFWG 201
>TC231485 similar to GB|AAN31116.1|23506209|AY149962 At3g06130/F28L1_7
{Arabidopsis thaliana;} , partial (9%)
Length = 636
Score = 28.1 bits (61), Expect = 8.6
Identities = 15/54 (27%), Positives = 26/54 (47%), Gaps = 6/54 (11%)
Frame = +3
Query: 2 RRSGSRRKKGNVSQPNSPSSSNIAFE------PHPSPKKSDQISRRLREPKLGP 49
+ SG+ KKG + +++ F PHP KS + S ++E ++GP
Sbjct: 132 QNSGAENKKGKEGKLEDHGNNDFDFHDYDDTPPHPKNGKSGKGSNNVKEGQMGP 293
>TC228673 similar to UP|Q8L8M8 (Q8L8M8) Serine-rich protein, partial (43%)
Length = 1083
Score = 28.1 bits (61), Expect = 8.6
Identities = 16/36 (44%), Positives = 21/36 (57%), Gaps = 3/36 (8%)
Frame = -2
Query: 151 EHEPPLL---KEEKEEENNEKQKPLDCVLDEKMCTE 183
E+E LL +EE+EEE PLD VL+E T+
Sbjct: 215 EYEQKLLECEREEEEEEEERSTGPLDFVLEELAITK 108
>TC229677 similar to UP|Q8VXD3 (Q8VXD3) N-acetylglucosaminyltransferase I ,
partial (32%)
Length = 438
Score = 28.1 bits (61), Expect = 8.6
Identities = 19/67 (28%), Positives = 34/67 (50%), Gaps = 4/67 (5%)
Frame = +2
Query: 52 EHLKENQKEDVKIAWRQLKLVY--DD--DIRLAQMPINCSFRLLRDIVKEKFPISRSVLI 107
++L+ + DVK+ W+ + L Y +D + A + + D+V + + I +V I
Sbjct: 110 QYLEPIKLNDVKVDWKSMDLSYLLEDKYSMHFANVIKKATPVYGADMVLKAYNIDGNVRI 289
Query: 108 KYKDNDD 114
KYKD D
Sbjct: 290 KYKDQSD 310
>TC221537 similar to UP|Q7XJJ2 (Q7XJJ2) Phosphoethanolamine
N-methyltransferase, partial (26%)
Length = 421
Score = 28.1 bits (61), Expect = 8.6
Identities = 15/36 (41%), Positives = 19/36 (52%), Gaps = 7/36 (19%)
Frame = +1
Query: 38 ISRRLREPKLGPTNEHLKENQ-------KEDVKIAW 66
IS R R+PK P H+KENQ ED ++ W
Sbjct: 127 ISTRRRDPKFCPYYHHMKENQL*NLEQVLEDSQLNW 234
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.316 0.134 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,403,333
Number of Sequences: 63676
Number of extensions: 225278
Number of successful extensions: 1474
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 1437
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1468
length of query: 459
length of database: 12,639,632
effective HSP length: 101
effective length of query: 358
effective length of database: 6,208,356
effective search space: 2222591448
effective search space used: 2222591448
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 61 (28.1 bits)
Medicago: description of AC122163.6