BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122162.9 - phase: 0
(380 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BU547704 72 5e-13
BF325009 67 2e-11
TC206896 weakly similar to UP|UTH1_YEAST (P36135) UTH1 protein, ... 36 0.033
CA853647 similar to GP|5732065|gb|A contains similarity to Medic... 32 0.47
TC204032 weakly similar to UP|Q9FZI1 (Q9FZI1) F1O19.5 protein, p... 31 0.81
TC232958 31 0.81
TC234025 homologue to UP|Q70I37 (Q70I37) Vacuolar proton-ATPase ... 31 0.81
TC215678 weakly similar to UP|Q9LH70 (Q9LH70) Gb|AAD22996.1, par... 31 0.81
TC227645 similar to UP|Q9FZK1 (Q9FZK1) F17L21.13, partial (61%) 30 1.4
TC206927 homologue to UP|Q9ZPK4 (Q9ZPK4) Glutamyl-tRNA reductase... 30 1.8
TC206782 weakly similar to UP|GLQ1_ARATH (Q9FJ62) Probable glyce... 30 1.8
TC222189 similar to UP|ITBX_DROME (P11584) Integrin beta-PS prec... 30 1.8
TC230574 30 2.4
BG404995 similar to GP|22831068|dbj OJ1131_E05.32 {Oryza sativa ... 30 2.4
BE823061 30 2.4
BM521628 28 5.2
TC212330 28 6.8
TC206781 weakly similar to UP|GLQ2_ARATH (Q9SZ11) Probable glyce... 28 6.8
TC222411 28 6.8
AW597560 28 6.8
>BU547704
Length = 600
Score = 71.6 bits (174), Expect = 5e-13
Identities = 41/100 (41%), Positives = 59/100 (59%), Gaps = 9/100 (9%)
Frame = -2
Query: 287 WVRLMDFD-----EKEKKWVKLRNFGDRVFFIGRGCSFSASASDLCIQKGNCAIFIDESV 341
W R + FD E+ KKWV+L + G+RV F+G C+FSASA DL + +GNC D+
Sbjct: 572 WERTVKFDVFXLDEEGKKWVELTDLGERVLFLGDDCAFSASAKDLNLGRGNCVAXRDDG- 396
Query: 342 LHNNNMVRGKRVFHLDQDRLSRGSK---YLNLFL-PPEWI 377
L N ++ G VF LD ++S S+ + LF PP+W+
Sbjct: 395 LGFNRVLNGMGVFRLDDGKISPLSECAGFSELFCPPPDWV 276
>BF325009
Length = 382
Score = 66.6 bits (161), Expect = 2e-11
Identities = 46/110 (41%), Positives = 58/110 (51%), Gaps = 11/110 (10%)
Frame = +2
Query: 212 CVFKGQLYAVHQSGQTVTIGP-DSSVELAAQPLGHDSPGGNKMLVESEGRLLLLGIEES- 269
CVFKG LY +G+TV + P D + LAA+P+ G K LVESEG LLL+ + S
Sbjct: 5 CVFKGNLYGADSNGRTVRVRPDDGGLTLAAEPV---FGGDKKFLVESEGALLLVDMYLSY 175
Query: 270 ----SNYFSIDFFELDEKKKKWVRLMDF-----DEKEKKWVKLRNFGDRV 310
F DF E D W R + F DE+ KKWV+L + DRV
Sbjct: 176 YSCTQGLFHEDFDEEDVAGMGWERTVKFDVFRLDEEGKKWVELTDLRDRV 325
>TC206896 weakly similar to UP|UTH1_YEAST (P36135) UTH1 protein, partial (4%)
Length = 807
Score = 35.8 bits (81), Expect = 0.033
Identities = 39/148 (26%), Positives = 56/148 (37%), Gaps = 12/148 (8%)
Frame = -1
Query: 65 YNDNEISS---------SSNHHPSQLEEKQTLIPRRPWLIRFTQNSHGKTKLFHPLIKYP 115
YN E++ +SN S L + P++PW I+ + + KL H L P
Sbjct: 639 YNSKELNGFSKYQTKFKASNEQYSSLASQDEPQPKQPWPIQPNETKAQQKKLEH*LQPKP 460
Query: 116 RPQQEETLAHPRVFDFNKLSVLHLGTDFILDKVNFTSRNRHGIYMLPKTVLAVTCHGKDP 175
P QE LA KL +LH+ N S N + + L+ + HG
Sbjct: 459 WP-QEPNLAWCSSIGTAKLPLLHI*WSHTC--YNSASPNPSNLKEAHSSSLSCSSHGSSS 289
Query: 176 LILGSLGYCSYRPLLFRDRDE---HWKP 200
+L CS+ P D WKP
Sbjct: 288 SVLS----CSHPPTFH*SSDPLILSWKP 217
>CA853647 similar to GP|5732065|gb|A contains similarity to Medicago
truncatula N7 protein (GB:Y17613) {Arabidopsis
thaliana}, partial (13%)
Length = 475
Score = 32.0 bits (71), Expect = 0.47
Identities = 14/39 (35%), Positives = 22/39 (55%)
Frame = +1
Query: 3 EADWSQLPKDLLTLISQRIDTEIDLIRFRSICSNWRSSS 41
E +W LP+D++ I Q++ L R + +CS WR S
Sbjct: 55 ERNWLDLPRDVVCTIFQKLGAIEILTRAQRVCSVWRGIS 171
>TC204032 weakly similar to UP|Q9FZI1 (Q9FZI1) F1O19.5 protein, partial (18%)
Length = 1500
Score = 31.2 bits (69), Expect = 0.81
Identities = 20/78 (25%), Positives = 38/78 (48%)
Frame = +2
Query: 205 SSLYGDTCVFKGQLYAVHQSGQTVTIGPDSSVELAAQPLGHDSPGGNKMLVESEGRLLLL 264
+SL G + + Y + G +V +V A G+D PG K+ ++S +LL
Sbjct: 368 TSLSGVAIIVENAAYLADKQGLSVI----DAVIGALSKAGYDKPGSQKVYIQSTNSSVLL 535
Query: 265 GIEESSNYFSIDFFELDE 282
+E ++Y + +++DE
Sbjct: 536 KFKEKTSYELV--YKIDE 583
>TC232958
Length = 758
Score = 31.2 bits (69), Expect = 0.81
Identities = 13/29 (44%), Positives = 21/29 (71%)
Frame = +1
Query: 9 LPKDLLTLISQRIDTEIDLIRFRSICSNW 37
LP+DL+T I R+ + L+RF+S+C +W
Sbjct: 61 LPQDLITEILLRLPVK-SLVRFKSVCKSW 144
>TC234025 homologue to UP|Q70I37 (Q70I37) Vacuolar proton-ATPase subunit-like
protein, partial (14%)
Length = 442
Score = 31.2 bits (69), Expect = 0.81
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +1
Query: 60 DNSIKYNDNEISSSSNHHPSQLEEKQTLIPRRPW 93
D S+ + + ++ S HHP ++ L+PRRPW
Sbjct: 88 DGSVPFGAD--ATGSAHHPHRIRSPHCLLPRRPW 183
>TC215678 weakly similar to UP|Q9LH70 (Q9LH70) Gb|AAD22996.1, partial (17%)
Length = 979
Score = 31.2 bits (69), Expect = 0.81
Identities = 26/87 (29%), Positives = 35/87 (39%), Gaps = 14/87 (16%)
Frame = +2
Query: 48 ILPFKFPLLKCEDNSIKYNDNEISSSSNHH-------PSQLEEKQTLI-------PRRPW 93
I P +PLL + +K N N I SSS+H PS EK + P+ P+
Sbjct: 197 ITPLWYPLLSYSTSHLKINKNNIPSSSSHDQGQEENLPSTYVEKCDIFSGEWVPNPKAPY 376
Query: 94 LIRFTQNSHGKTKLFHPLIKYPRPQQE 120
+T + IKY RP E
Sbjct: 377 ---YTNKTCWAIHEHQNCIKYGRPDSE 448
>TC227645 similar to UP|Q9FZK1 (Q9FZK1) F17L21.13, partial (61%)
Length = 1671
Score = 30.4 bits (67), Expect = 1.4
Identities = 13/39 (33%), Positives = 18/39 (45%)
Frame = +3
Query: 1 MAEADWSQLPKDLLTLISQRIDTEIDLIRFRSICSNWRS 39
M + W P+DL + R+ RFRS+C W S
Sbjct: 339 MEQEIWKDFPEDLFEAVIARLPIST-FFRFRSVCRQWNS 452
>TC206927 homologue to UP|Q9ZPK4 (Q9ZPK4) Glutamyl-tRNA reductase, partial
(69%)
Length = 1499
Score = 30.0 bits (66), Expect = 1.8
Identities = 17/60 (28%), Positives = 31/60 (51%)
Frame = -1
Query: 19 QRIDTEIDLIRFRSICSNWRSSSIPNHHNILPFKFPLLKCEDNSIKYNDNEISSSSNHHP 78
+R +++++F + CSN SSI NHH F + D+ + + ++S +N HP
Sbjct: 521 KRFVDNLNILQFFTYCSNSLLSSIDNHHLFATFCHQMF---DHKLSH----LASPNNQHP 363
>TC206782 weakly similar to UP|GLQ1_ARATH (Q9FJ62) Probable glycerophosphoryl
diester phosphodiesterase 1 precursor , partial (21%)
Length = 588
Score = 30.0 bits (66), Expect = 1.8
Identities = 19/78 (24%), Positives = 37/78 (47%)
Frame = +3
Query: 205 SSLYGDTCVFKGQLYAVHQSGQTVTIGPDSSVELAAQPLGHDSPGGNKMLVESEGRLLLL 264
+SL G + Y + +VT ++ A G+D PG K++++S +LL
Sbjct: 123 TSLLGVVITIENAAYLARKQNLSVTEAVIDTLSKA----GYDKPGAPKVMIQSTNSSVLL 290
Query: 265 GIEESSNYFSIDFFELDE 282
+E + Y + +++DE
Sbjct: 291 KFKEKTKYELV--YKIDE 338
>TC222189 similar to UP|ITBX_DROME (P11584) Integrin beta-PS precursor
(Position-specific antigen, beta chain) (Myospheroid
protein) (Olfactory C protein), partial (3%)
Length = 1477
Score = 30.0 bits (66), Expect = 1.8
Identities = 13/40 (32%), Positives = 23/40 (57%)
Frame = +3
Query: 266 IEESSNYFSIDFFELDEKKKKWVRLMDFDEKEKKWVKLRN 305
+EE + + FEL++++ KW R E+E + KL+N
Sbjct: 1107 LEEQQVSYQMQAFELEKQRLKWARFSSKKEREMEKDKLQN 1226
>TC230574
Length = 848
Score = 29.6 bits (65), Expect = 2.4
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +3
Query: 286 KWVRLMDFDEKEKKWVKLRNFGDRV 310
+WV+ DFDE KK ++RN DR+
Sbjct: 441 EWVKRQDFDELRKKMKEIRNERDRL 515
>BG404995 similar to GP|22831068|dbj OJ1131_E05.32 {Oryza sativa (japonica
cultivar-group)}, partial (8%)
Length = 394
Score = 29.6 bits (65), Expect = 2.4
Identities = 18/55 (32%), Positives = 27/55 (48%)
Frame = +1
Query: 9 LPKDLLTLISQRIDTEIDLIRFRSICSNWRSSSIPNHHNILPFKFPLLKCEDNSI 63
LP+++LT I R+ L+RFRS +W+S H N L L + S+
Sbjct: 40 LPREVLTEILSRLPVR-SLLRFRSTSKSWKSLIDSQHLNWLHLTRSLTLASNTSL 201
>BE823061
Length = 626
Score = 29.6 bits (65), Expect = 2.4
Identities = 14/46 (30%), Positives = 23/46 (49%)
Frame = -3
Query: 260 RLLLLGIEESSNYFSIDFFELDEKKKKWVRLMDFDEKEKKWVKLRN 305
R ++ +EE FEL++++ KW R E+E + KL N
Sbjct: 543 RSRIMQLEEQQISXHTQAFELEKQRLKWARFSSKKEREMETAKLEN 406
>BM521628
Length = 428
Score = 28.5 bits (62), Expect = 5.2
Identities = 26/87 (29%), Positives = 38/87 (42%), Gaps = 2/87 (2%)
Frame = +1
Query: 44 NHHNILPFKFPLLKCEDNSIKYNDNEISSSSNHHP--SQLEEKQTLIPRRPWLIRFTQNS 101
N+ +I L +S K N+N +S+ +HHP + +Q IP P L
Sbjct: 136 NYTHIYIHSLFLSSSSSSSSKTNNNGAASNPHHHP**HEPRNQQWRIPFLPRL------- 294
Query: 102 HGKTKLFHPLIKYPRPQQEETLAHPRV 128
+ L HPL + RP Q A PR+
Sbjct: 295 --RQHLLHPLRQ--RPFQPRPWAPPRL 363
>TC212330
Length = 428
Score = 28.1 bits (61), Expect = 6.8
Identities = 15/49 (30%), Positives = 23/49 (46%), Gaps = 3/49 (6%)
Frame = +2
Query: 3 EADWSQLPKDLLTLISQRIDTEIDLIRFRSICSNWR---SSSIPNHHNI 48
EA+ +LP DLL I + DL + +C W+ S+ HN+
Sbjct: 77 EAEIDRLPIDLLAHIFVLFTSFTDLAQASGVCKKWKQGVKESLARRHNL 223
>TC206781 weakly similar to UP|GLQ2_ARATH (Q9SZ11) Probable glycerophosphoryl
diester phosphodiesterase 2 precursor , partial (24%)
Length = 1111
Score = 28.1 bits (61), Expect = 6.8
Identities = 12/39 (30%), Positives = 24/39 (60%)
Frame = +2
Query: 244 GHDSPGGNKMLVESEGRLLLLGIEESSNYFSIDFFELDE 282
G+D PG K++++S +LL +E + Y + +++DE
Sbjct: 98 GYDKPGAPKVMIQSTNSSVLLKFKEKTKYELV--YKIDE 208
>TC222411
Length = 968
Score = 28.1 bits (61), Expect = 6.8
Identities = 18/44 (40%), Positives = 24/44 (53%)
Frame = -3
Query: 166 LAVTCHGKDPLILGSLGYCSYRPLLFRDRDEHWKPIPKMSSLYG 209
L +T +GK PLILG+ + PL R WK I +S +YG
Sbjct: 531 LLLTSYGKHPLILGTRTF-EQSPLALRT----WKRISCISLVYG 415
>AW597560
Length = 247
Score = 28.1 bits (61), Expect = 6.8
Identities = 11/35 (31%), Positives = 23/35 (65%)
Frame = +3
Query: 270 SNYFSIDFFELDEKKKKWVRLMDFDEKEKKWVKLR 304
+ +FS + +L++KKKKW + F+ + +K + L+
Sbjct: 30 AQFFSQEKKQLEKKKKKWKMVRKFENQSRKIMALQ 134
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.139 0.432
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,209,201
Number of Sequences: 63676
Number of extensions: 355813
Number of successful extensions: 1733
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 1717
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1729
length of query: 380
length of database: 12,639,632
effective HSP length: 99
effective length of query: 281
effective length of database: 6,335,708
effective search space: 1780333948
effective search space used: 1780333948
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
Medicago: description of AC122162.9