BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122162.7 - phase: 0
(377 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BU547704 89 4e-18
BF325009 71 7e-13
CA853647 similar to GP|5732065|gb|A contains similarity to Medic... 33 0.21
TC209643 31 0.80
TC213738 30 1.4
BM528781 similar to GP|21592984|gb| photolyase/blue-light recept... 30 2.3
BG404995 similar to GP|22831068|dbj OJ1131_E05.32 {Oryza sativa ... 30 2.3
TC220558 similar to UP|Q9M3H9 (Q9M3H9) Tubby-like protein, parti... 29 3.0
TC227645 similar to UP|Q9FZK1 (Q9FZK1) F17L21.13, partial (61%) 29 4.0
NP595172 polyprotein [Glycine max] 29 4.0
TC207113 weakly similar to UP|GLO2_YEAST (Q05584) Hydroxyacylglu... 28 6.8
TC211243 28 6.8
TC204253 28 6.8
TC205465 weakly similar to GB|AAP42752.1|30984578|BT008739 At1g5... 28 6.8
TC205464 weakly similar to GB|AAP42752.1|30984578|BT008739 At1g5... 28 6.8
BM121547 28 8.9
BF425343 weakly similar to GP|19715593|gb| AT5g01740/T20L15_10 {... 28 8.9
BU926447 similar to GP|8453100|gb|A short-root protein {Arabidop... 28 8.9
TC227676 28 8.9
TC212571 similar to UP|Q9FF18 (Q9FF18) Cytochrome P450-like prot... 28 8.9
>BU547704
Length = 600
Score = 88.6 bits (218), Expect = 4e-18
Identities = 48/99 (48%), Positives = 64/99 (64%), Gaps = 2/99 (2%)
Frame = -2
Query: 276 VWIDVFRLDEKEKRWVKLANLGDKVLFLGNGYSFSASASELGFANGNCVIY-SKSYGFHD 334
V DVF LDE+ K+WV+L +LG++VLFLG+ +FSASA +L GNCV GF+
Sbjct: 560 VKFDVFXLDEEGKKWVELTDLGERVLFLGDDCAFSASAKDLNLGRGNCVAXRDDGLGFNR 381
Query: 335 -LNIRKCKMSVFHLDQGQASPLSDYPEYFKLFWPPPEWI 372
LN M VF LD G+ SPLS+ + +LF PPP+W+
Sbjct: 380 VLN----GMGVFRLDDGKISPLSECAGFSELFCPPPDWV 276
>BF325009
Length = 382
Score = 71.2 bits (173), Expect = 7e-13
Identities = 51/111 (45%), Positives = 58/111 (51%), Gaps = 25/111 (22%)
Frame = +2
Query: 215 VCVFKGRPCVVDKTGRTFMIGPD---LTTHLIAEPVFGGKSKFLVESSEFELLLVDRYEN 271
VCVFKG D GRT + PD LT L AEPVFGG KFLVES E LLLVD Y +
Sbjct: 2 VCVFKGNLYGADSNGRTVRVRPDDGGLT--LAAEPVFGGDKKFLVES-EGALLLVDMYLS 172
Query: 272 Y----------------------RVPVWIDVFRLDEKEKRWVKLANLGDKV 300
Y V DVFRLDE+ K+WV+L +L D+V
Sbjct: 173 YYSCTQGLFHEDFDEEDVAGMGWERTVKFDVFRLDEEGKKWVELTDLRDRV 325
>CA853647 similar to GP|5732065|gb|A contains similarity to Medicago
truncatula N7 protein (GB:Y17613) {Arabidopsis
thaliana}, partial (13%)
Length = 475
Score = 33.1 bits (74), Expect = 0.21
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = +1
Query: 3 DWSHLPSELLQLISQKLDTVLCLLRFRSVCSTWRSSS 39
+W LP +++ I QKL + L R + VCS WR S
Sbjct: 61 NWLDLPRDVVCTIFQKLGAIEILTRAQRVCSVWRGIS 171
>TC209643
Length = 1256
Score = 31.2 bits (69), Expect = 0.80
Identities = 25/114 (21%), Positives = 44/114 (37%), Gaps = 11/114 (9%)
Frame = -2
Query: 22 VLCLLRFRSVCSTWRSSSIPNHKHKNSSPLNIPQFPEPNFDPFNIRNIIKSKYITSRLIT 81
+ C ++F +CS+W S+P H H F FD +++R + ++ +
Sbjct: 343 IFCRVQFL-ICSSWHYQSLPIHHH--------AMFVAAKFDQYHLRQRQRWAMLSGLIPP 191
Query: 82 HTIFLIKPPQ---------HQPS--LNPWLIRIGPDFKGKTQLWHPFSSGQNLS 124
T FL+ H P ++PW P + WH F ++S
Sbjct: 190 VTAFLLLLQSLLLLLDRICHHPQSLVSPWCEMPEPFLVSQYH*WHLFLKSLSVS 29
>TC213738
Length = 690
Score = 30.4 bits (67), Expect = 1.4
Identities = 17/40 (42%), Positives = 21/40 (52%)
Frame = -1
Query: 22 VLCLLRFRSVCSTWRSSSIPNHKHKNSSPLNIPQFPEPNF 61
V CLLR + VC +W S IP+ + S L P P NF
Sbjct: 660 VRCLLRLKYVCKSW-LSLIPDPQFAKSPILT*PLHPPTNF 544
>BM528781 similar to GP|21592984|gb| photolyase/blue-light receptor PHR2
{Arabidopsis thaliana}, partial (13%)
Length = 331
Score = 29.6 bits (65), Expect = 2.3
Identities = 27/83 (32%), Positives = 36/83 (42%), Gaps = 4/83 (4%)
Frame = +2
Query: 27 RFRSVCSTWRSSSIPNHKHKNSSPLNIPQF-PEPNFDPFNIRNIIKSKYITSRLITHTI- 84
R R+ S W+ S+ +H SP P+F P N P N + SK S +HT
Sbjct: 14 R*RA*LSKWKQRSMKHHWQWPQSPSLSPEFCPSSNPPPLNQPS---SKSQPSPHPSHTFL 184
Query: 85 --FLIKPPQHQPSLNPWLIRIGP 105
L KPP + PS P + P
Sbjct: 185 SPLLPKPPSNPPSPPPPFTPLSP 253
>BG404995 similar to GP|22831068|dbj OJ1131_E05.32 {Oryza sativa (japonica
cultivar-group)}, partial (8%)
Length = 394
Score = 29.6 bits (65), Expect = 2.3
Identities = 16/32 (50%), Positives = 20/32 (62%)
Frame = +1
Query: 6 HLPSELLQLISQKLDTVLCLLRFRSVCSTWRS 37
HLP E+L I +L V LLRFRS +W+S
Sbjct: 37 HLPREVLTEILSRLP-VRSLLRFRSTSKSWKS 129
>TC220558 similar to UP|Q9M3H9 (Q9M3H9) Tubby-like protein, partial (42%)
Length = 629
Score = 29.3 bits (64), Expect = 3.0
Identities = 28/91 (30%), Positives = 41/91 (44%), Gaps = 19/91 (20%)
Frame = +2
Query: 4 WSHLPSELLQLISQKLD-----------TVLCLLRFRSVCSTWRSSS-----IPNHKHKN 47
W++LPSELL I Q+++ V C SVC +WRS + P +
Sbjct: 266 WANLPSELLLDIIQRVEESETSWPARAVVVFC----ASVCKSWRSITREIVKTPEQCGRI 433
Query: 48 SSPLNIPQFPEPNFDP---FNIRNIIKSKYI 75
+ P+++ Q P P P F RN S Y+
Sbjct: 434 TFPISLKQ-PGPRDSPIQCFIRRNKETSTYL 523
>TC227645 similar to UP|Q9FZK1 (Q9FZK1) F17L21.13, partial (61%)
Length = 1671
Score = 28.9 bits (63), Expect = 4.0
Identities = 12/34 (35%), Positives = 17/34 (49%)
Frame = +3
Query: 4 WSHLPSELLQLISQKLDTVLCLLRFRSVCSTWRS 37
W P +L + + +L + RFRSVC W S
Sbjct: 354 WKDFPEDLFEAVIARLP-ISTFFRFRSVCRQWNS 452
>NP595172 polyprotein [Glycine max]
Length = 4659
Score = 28.9 bits (63), Expect = 4.0
Identities = 15/41 (36%), Positives = 19/41 (45%)
Frame = +1
Query: 28 FRSVCSTWRSSSIPNHKHKNSSPLNIPQFPEPNFDPFNIRN 68
F S S + N K+ N P P P P+ PFN+RN
Sbjct: 799 FTSNTSATQKYPPTNQKNDNPKPNLPPLLPTPSTKPFNLRN 921
>TC207113 weakly similar to UP|GLO2_YEAST (Q05584) Hydroxyacylglutathione
hydrolase, cytoplasmic isozyme (Glyoxalase II) (Glx II)
, partial (8%)
Length = 679
Score = 28.1 bits (61), Expect = 6.8
Identities = 19/46 (41%), Positives = 25/46 (54%)
Frame = -1
Query: 14 LISQKLDTVLCLLRFRSVCSTWRSSSIPNHKHKNSSPLNIPQFPEP 59
L+S D +L LR S+CS S S+P+ K SP+ I PEP
Sbjct: 334 LVSSGKDMIL--LREDSICSGVPSKSLPHDKLNKVSPVKIA--PEP 209
>TC211243
Length = 1069
Score = 28.1 bits (61), Expect = 6.8
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +1
Query: 267 DRYENYRVPVWIDVFRLDE 285
D YEN+ P+W+D+ DE
Sbjct: 223 DTYENFGAPMWVDLSAFDE 279
>TC204253
Length = 1300
Score = 28.1 bits (61), Expect = 6.8
Identities = 16/42 (38%), Positives = 20/42 (47%)
Frame = -1
Query: 101 IRIGPDFKGKTQLWHPFSSGQNLSSDFNNVLDFNKLSFRVLG 142
IR F + QLWH SSG N DFN + S+ + G
Sbjct: 964 IRSSVPFHWRYQLWHSHSSGNN---DFNAFSNGKIWSYNIFG 848
>TC205465 weakly similar to GB|AAP42752.1|30984578|BT008739 At1g57790
{Arabidopsis thaliana;} , partial (15%)
Length = 490
Score = 28.1 bits (61), Expect = 6.8
Identities = 15/34 (44%), Positives = 21/34 (61%)
Frame = +3
Query: 4 WSHLPSELLQLISQKLDTVLCLLRFRSVCSTWRS 37
WS LP+ELL+LI +L ++ +R VC W S
Sbjct: 177 WSDLPTELLELILSRL-SLDDNVRASVVCKRWHS 275
>TC205464 weakly similar to GB|AAP42752.1|30984578|BT008739 At1g57790
{Arabidopsis thaliana;} , partial (17%)
Length = 756
Score = 28.1 bits (61), Expect = 6.8
Identities = 15/34 (44%), Positives = 21/34 (61%)
Frame = +1
Query: 4 WSHLPSELLQLISQKLDTVLCLLRFRSVCSTWRS 37
WS LP+ELL+LI +L ++ +R VC W S
Sbjct: 181 WSDLPTELLELILSRL-SLDDNVRASVVCKRWHS 279
>BM121547
Length = 896
Score = 27.7 bits (60), Expect = 8.9
Identities = 23/100 (23%), Positives = 40/100 (40%), Gaps = 5/100 (5%)
Frame = +2
Query: 31 VCSTWRSSSIPNHKHKNSSPLNIPQFPEPNFD-----PFNIRNIIKSKYITSRLITHTIF 85
+ + W+S +IP ++ + PL++P P F P ++ +II T ++
Sbjct: 95 ISNAWKSIAIPTFGYEGNLPLSLPSIAIPTFGYEGNLPLSLPSIITR--------TRSLT 250
Query: 86 LIKPPQHQPSLNPWLIRIGPDFKGKTQLWHPFSSGQNLSS 125
L P H P I K ++P NLS+
Sbjct: 251 LALPQNHGSEALPMYHFINATETEKLD-YNPKKRASNLSA 367
>BF425343 weakly similar to GP|19715593|gb| AT5g01740/T20L15_10 {Arabidopsis
thaliana}, partial (50%)
Length = 371
Score = 27.7 bits (60), Expect = 8.9
Identities = 12/30 (40%), Positives = 17/30 (56%), Gaps = 3/30 (10%)
Frame = -1
Query: 161 IHCVAAVCRGEQPPVVVTC---GEFTHCDI 187
+H V A+ R +PP+ VTC G HC +
Sbjct: 194 LHHVVAMRRAMEPPLKVTC*QFGHIVHCSL 105
>BU926447 similar to GP|8453100|gb|A short-root protein {Arabidopsis
thaliana}, partial (7%)
Length = 450
Score = 27.7 bits (60), Expect = 8.9
Identities = 16/47 (34%), Positives = 21/47 (44%)
Frame = +2
Query: 91 QHQPSLNPWLIRIGPDFKGKTQLWHPFSSGQNLSSDFNNVLDFNKLS 137
Q PSLN R + Q ++P+SS Q FN +D LS
Sbjct: 41 QPDPSLNSTTSRTSSSSRSSRQNYYPYSSYQEEEECFNFYMDEEDLS 181
>TC227676
Length = 1104
Score = 27.7 bits (60), Expect = 8.9
Identities = 20/75 (26%), Positives = 33/75 (43%), Gaps = 1/75 (1%)
Frame = +1
Query: 25 LLRFRSVCSTWRSSSIPNHKHKNSSPLNIPQFPEPNFDPFNIRNIIKSKYITSRLIT-HT 83
LL S+ S ++ HKH+ + P PEP P ++++ + RL + H
Sbjct: 238 LLPIPSLLQRVTSFNLSLHKHQPTPPETQHLHPEPQPQPEKPPELLRTPSLLERLRSFHN 417
Query: 84 IFLIKPPQHQPSLNP 98
+ L K Q +P P
Sbjct: 418 LSLHKHVQPEPEPEP 462
>TC212571 similar to UP|Q9FF18 (Q9FF18) Cytochrome P450-like protein, partial
(48%)
Length = 883
Score = 27.7 bits (60), Expect = 8.9
Identities = 20/65 (30%), Positives = 29/65 (43%), Gaps = 2/65 (3%)
Frame = +2
Query: 262 ELLLVDRYENYRVPVWIDVFRLDEKEKRWVKLANLGDKVLFLGNGYSFSASASELGFANG 321
+++L D Y + +WI V + EK W K AN + F + L FA+G
Sbjct: 491 DIVLGDLYIPKGLSIWIPVLAIHHSEKLWGKDANEFNPERFTSKSF---VPGRFLPFASG 661
Query: 322 --NCV 324
NCV
Sbjct: 662 PRNCV 676
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.324 0.140 0.458
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,081,392
Number of Sequences: 63676
Number of extensions: 409595
Number of successful extensions: 2011
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 1994
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2008
length of query: 377
length of database: 12,639,632
effective HSP length: 99
effective length of query: 278
effective length of database: 6,335,708
effective search space: 1761326824
effective search space used: 1761326824
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 60 (27.7 bits)
Medicago: description of AC122162.7