BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122162.12 - phase: 0
(400 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BU547704 79 4e-15
BF325009 64 9e-11
TC205467 37 0.016
CA853647 similar to GP|5732065|gb|A contains similarity to Medic... 34 0.10
TC205513 similar to UP|Q84TL1 (Q84TL1) Glycine decarboxylase com... 33 0.30
TC227645 similar to UP|Q9FZK1 (Q9FZK1) F17L21.13, partial (61%) 32 0.51
TC205465 weakly similar to GB|AAP42752.1|30984578|BT008739 At1g5... 31 0.87
TC232958 31 0.87
TC205464 weakly similar to GB|AAP42752.1|30984578|BT008739 At1g5... 31 0.87
TC205514 similar to UP|Q84TL1 (Q84TL1) Glycine decarboxylase com... 30 2.5
TC230489 similar to UP|Q8LEE4 (Q8LEE4) Zinc finger protein, part... 29 3.3
AI443156 29 3.3
TC229031 weakly similar to UP|Q8VBJ3 (Q8VBJ3) Core protein, part... 29 3.3
TC227131 similar to UP|Q8GSD7 (Q8GSD7) MRNA capping enzyme-like ... 29 3.3
TC228621 similar to UP|Q8RXD8 (Q8RXD8) WD repeat protein-like, p... 29 4.3
TC205468 weakly similar to GB|AAC35522.1|3600034|T12H20 T12H20.2... 29 4.3
TC231109 weakly similar to UP|Q9LUP7 (Q9LUP7) Gb|AAD25583.1, par... 28 5.6
AW395660 28 7.3
TC225285 similar to UP|Q9ZS51 (Q9ZS51) PEROXISOMAL MEMBRANE PROT... 28 7.3
BG404995 similar to GP|22831068|dbj OJ1131_E05.32 {Oryza sativa ... 28 9.6
>BU547704
Length = 600
Score = 79.0 bits (193), Expect = 4e-15
Identities = 47/106 (44%), Positives = 63/106 (59%), Gaps = 3/106 (2%)
Frame = -2
Query: 295 DIHQTFFQFSIKF--FKLDEMEKKWVKLKKLGGRVLFVGSGCSFSASGWDLCLPKGNCVI 352
D+ ++ ++KF F LDE KKWV+L LG RVLF+G C+FSAS DL L +GNCV
Sbjct: 590 DVAGMGWERTVKFDVFXLDEEGKKWVELTDLGERVLFLGDDCAFSASAKDLNLGRGNCVA 411
Query: 353 FIDTSVLSSDNMAGGNRVFHLDRGQLSHVSEYPECENLFL-PPEWI 397
D L + + G VF LD G++S +SE LF PP+W+
Sbjct: 410 XRDDG-LGFNRVLNGMGVFRLDDGKISPLSECAGFSELFCPPPDWV 276
>BF325009
Length = 382
Score = 64.3 bits (155), Expect = 9e-11
Identities = 41/113 (36%), Positives = 62/113 (54%), Gaps = 24/113 (21%)
Frame = +2
Query: 239 ICLFKGRFYVVDQSGQTVTVGP-DSSTELAAQPLYRRCVRGENRKLLVENEGELLLLDIH 297
+C+FKG Y D +G+TV V P D LAA+P++ G ++K LVE+EG LLL+D++
Sbjct: 2 VCVFKGNLYGADSNGRTVRVRPDDGGLTLAAEPVF-----GGDKKFLVESEGALLLVDMY 166
Query: 298 QTFF---------------------QFSIKF--FKLDEMEKKWVKLKKLGGRV 327
+++ + ++KF F+LDE KKWV+L L RV
Sbjct: 167 LSYYSCTQGLFHEDFDEEDVAGMGWERTVKFDVFRLDEEGKKWVELTDLRDRV 325
>TC205467
Length = 1504
Score = 37.0 bits (84), Expect = 0.016
Identities = 69/326 (21%), Positives = 124/326 (37%), Gaps = 4/326 (1%)
Frame = +1
Query: 8 VATSSMASVDWSELPKDIIFLISQRLDVELDLIRFRSVCSTWRSSPIPNDHNILPFQFPL 67
V + ++ W++LP +++ I RL + D IR +VC W S + +D ++ Q P
Sbjct: 7 VKSENLEMQSWADLPAELLESILSRL-ILADNIRASAVCRRWHS--VASDVRVVN-QSPW 174
Query: 68 LKYVPTPDSISNNNEIIDNIENTSTSFG--HLSKRSFFLVKPPQEQQQQETLIRRRPWLI 125
L Y P + E D ++ + +F L+ K L+ RP
Sbjct: 175 LMYFP---KFGDCYEFYDPVQRKTHTFELPELNGSRVCYTK-------DGWLLLYRPRTH 324
Query: 126 RITQNSSGKTQLLKPPFLSLTS--TAFSHLPNVVDFTKFSPQHLATDFIIDKDDLTFQNQ 183
R+ + +L+K P +T AFS P F+ +H++ + +
Sbjct: 325 RVFFFNPFTRELIKLPRFEMTYQIVAFSCAPTSPGCVLFTVKHVSPTVVAISTCYPGATE 504
Query: 184 HSSYLYPQKILAVTCPEKKPLVLGTLSYCSSKRVLFHDRDKRWTPVSNLSTAHGDICLFK 243
++ Y ++ V+ K + L YC S + +
Sbjct: 505 WTTVNYQNRLPFVSSIWNKLVFCNGLFYCLS------------------------LTGWL 612
Query: 244 GRFYVVDQSGQTVTVGPDSSTELAAQPLYRRCVRGENRKLLVENEGELLLLDIHQTFFQF 303
G F V+ + + V P E + +G K + E+EG++L++ T
Sbjct: 613 GVFDPVECTWSVLAVPPPKCPE---NFFAKNWWKG---KFMAEHEGDILVI---YTCCSE 765
Query: 304 SIKFFKLDEMEKKWVKLKKLGGRVLF 329
+ FKLD+ KW ++ L G LF
Sbjct: 766 NPIIFKLDQTLMKWEEMTTLDGVTLF 843
>CA853647 similar to GP|5732065|gb|A contains similarity to Medicago
truncatula N7 protein (GB:Y17613) {Arabidopsis
thaliana}, partial (13%)
Length = 475
Score = 34.3 bits (77), Expect = 0.10
Identities = 16/44 (36%), Positives = 23/44 (51%)
Frame = +1
Query: 7 AVATSSMASVDWSELPKDIIFLISQRLDVELDLIRFRSVCSTWR 50
AV +W +LP+D++ I Q+L L R + VCS WR
Sbjct: 31 AVVADCEEERNWLDLPRDVVCTIFQKLGAIEILTRAQRVCSVWR 162
>TC205513 similar to UP|Q84TL1 (Q84TL1) Glycine decarboxylase complex
H-protein, complete
Length = 708
Score = 32.7 bits (73), Expect = 0.30
Identities = 28/72 (38%), Positives = 36/72 (49%), Gaps = 9/72 (12%)
Frame = -3
Query: 84 IDNIENTSTSFGH--LSKR--SFFLVKPPQEQQQQETLIRRRPWLIRITQNSSGKTQLLK 139
I +I++ GH L +R +F + P E Q L + L TQ SSGK QLLK
Sbjct: 229 IQHIQHHLNDLGHDQLCRRLQNFHQLSPTHENQHTSNL*QH*KSLCE-TQRSSGKRQLLK 53
Query: 140 P-----PFLSLT 146
P PFLSL+
Sbjct: 52 PTIACKPFLSLS 17
>TC227645 similar to UP|Q9FZK1 (Q9FZK1) F17L21.13, partial (61%)
Length = 1671
Score = 32.0 bits (71), Expect = 0.51
Identities = 15/42 (35%), Positives = 20/42 (46%)
Frame = +3
Query: 10 TSSMASVDWSELPKDIIFLISQRLDVELDLIRFRSVCSTWRS 51
T M W + P+D+ + RL + RFRSVC W S
Sbjct: 330 TEVMEQEIWKDFPEDLFEAVIARLPIST-FFRFRSVCRQWNS 452
>TC205465 weakly similar to GB|AAP42752.1|30984578|BT008739 At1g57790
{Arabidopsis thaliana;} , partial (15%)
Length = 490
Score = 31.2 bits (69), Expect = 0.87
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +3
Query: 18 WSELPKDIIFLISQRLDVELDLIRFRSVCSTWRS 51
WS+LP +++ LI RL ++ D +R VC W S
Sbjct: 177 WSDLPTELLELILSRLSLD-DNVRASVVCKRWHS 275
>TC232958
Length = 758
Score = 31.2 bits (69), Expect = 0.87
Identities = 15/29 (51%), Positives = 21/29 (71%)
Frame = +1
Query: 21 LPKDIIFLISQRLDVELDLIRFRSVCSTW 49
LP+D+I I RL V+ L+RF+SVC +W
Sbjct: 61 LPQDLITEILLRLPVK-SLVRFKSVCKSW 144
>TC205464 weakly similar to GB|AAP42752.1|30984578|BT008739 At1g57790
{Arabidopsis thaliana;} , partial (17%)
Length = 756
Score = 31.2 bits (69), Expect = 0.87
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +1
Query: 18 WSELPKDIIFLISQRLDVELDLIRFRSVCSTWRS 51
WS+LP +++ LI RL ++ D +R VC W S
Sbjct: 181 WSDLPTELLELILSRLSLD-DNVRASVVCKRWHS 279
>TC205514 similar to UP|Q84TL1 (Q84TL1) Glycine decarboxylase complex
H-protein, complete
Length = 755
Score = 29.6 bits (65), Expect = 2.5
Identities = 27/72 (37%), Positives = 33/72 (45%), Gaps = 9/72 (12%)
Frame = -2
Query: 84 IDNIENTSTSFGHLSK----RSFFLVKPPQEQQQQETLIRRRPWLIRITQNSSGKTQLLK 139
I +I++ GH R+F + P E Q L L TQ SSGK QLLK
Sbjct: 238 IQHIQHHPNDLGHDQLCRWLRNFHQLSPTHENQHT*NL**H*KSLCE-TQRSSGKRQLLK 62
Query: 140 P-----PFLSLT 146
P PFLSL+
Sbjct: 61 PTIACKPFLSLS 26
>TC230489 similar to UP|Q8LEE4 (Q8LEE4) Zinc finger protein, partial (8%)
Length = 948
Score = 29.3 bits (64), Expect = 3.3
Identities = 20/53 (37%), Positives = 28/53 (52%), Gaps = 3/53 (5%)
Frame = +1
Query: 350 CVIFIDTSVLSSDNMAGGNRVFHLDRGQ--LSHVSEYPECENL-FLPPEWILK 399
C ++T LSS++M G +FHL Q L +Y N+ FLP +W LK
Sbjct: 397 CSKGVNTHFLSSEDMCGIILIFHL*IYQIGLQRYVQYQTSVNVTFLPSDWSLK 555
>AI443156
Length = 414
Score = 29.3 bits (64), Expect = 3.3
Identities = 21/54 (38%), Positives = 30/54 (54%)
Frame = +3
Query: 8 VATSSMASVDWSELPKDIIFLISQRLDVELDLIRFRSVCSTWRSSPIPNDHNIL 61
+ S+MA+ LP +++ I RL V+ +IR RS C WRS I + H IL
Sbjct: 66 ITVSNMAN-----LPVEVVTEILSRLPVK-SVIRLRSTCKWWRSI-IDSRHFIL 206
>TC229031 weakly similar to UP|Q8VBJ3 (Q8VBJ3) Core protein, partial (10%)
Length = 694
Score = 29.3 bits (64), Expect = 3.3
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = +1
Query: 163 SPQHLATDFIIDKDDLTFQNQHSSYLYPQKILAVTCP 199
S H TDF+ + T +N HS Y ++A CP
Sbjct: 550 SGSHF*TDFLYKRHSTTNKNSHSIIPYHSPLVATECP 660
>TC227131 similar to UP|Q8GSD7 (Q8GSD7) MRNA capping enzyme-like protein,
partial (38%)
Length = 1812
Score = 29.3 bits (64), Expect = 3.3
Identities = 17/42 (40%), Positives = 22/42 (51%)
Frame = -1
Query: 24 DIIFLISQRLDVELDLIRFRSVCSTWRSSPIPNDHNILPFQF 65
D++ SQ L V + LIR +CS D +ILPFQF
Sbjct: 894 DVVSYASQHLPVSIKLIRS*FICS---------DSHILPFQF 796
>TC228621 similar to UP|Q8RXD8 (Q8RXD8) WD repeat protein-like, partial (70%)
Length = 1014
Score = 28.9 bits (63), Expect = 4.3
Identities = 16/62 (25%), Positives = 28/62 (44%)
Frame = -1
Query: 153 LPNVVDFTKFSPQHLATDFIIDKDDLTFQNQHSSYLYPQKILAVTCPEKKPLVLGTLSYC 212
LP++++FT F+ + I K ++ + +YP K +A T P + G C
Sbjct: 543 LPSLLNFTTFASEISPPTENISKGPFSYLRASNILMYPPKAIAKTSPGSSYDIAGLPCTC 364
Query: 213 SS 214
S
Sbjct: 363 KS 358
>TC205468 weakly similar to GB|AAC35522.1|3600034|T12H20 T12H20.2
{Arabidopsis thaliana;} , partial (21%)
Length = 867
Score = 28.9 bits (63), Expect = 4.3
Identities = 20/65 (30%), Positives = 34/65 (51%)
Frame = +2
Query: 282 KLLVENEGELLLLDIHQTFFQFSIKFFKLDEMEKKWVKLKKLGGRVLFVGSGCSFSASGW 341
K + E+EG+++++ T + FKLD+M +W ++ L G LF SF +S
Sbjct: 236 KFMTEHEGDIIVI---YTCSSENPIIFKLDQMLMEWEEMTTLDGVTLF----ASFLSSHA 394
Query: 342 DLCLP 346
+ LP
Sbjct: 395 RIDLP 409
>TC231109 weakly similar to UP|Q9LUP7 (Q9LUP7) Gb|AAD25583.1, partial (8%)
Length = 1061
Score = 28.5 bits (62), Expect = 5.6
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +3
Query: 19 SELPKDIIFLISQRLDVELDLIRFRSVCSTWRS 51
+ LP +++ I RL V+ +IR RS C WRS
Sbjct: 87 ANLPVEVVTEILSRLPVK-SVIRLRSTCKWWRS 182
>AW395660
Length = 381
Score = 28.1 bits (61), Expect = 7.3
Identities = 13/31 (41%), Positives = 20/31 (63%)
Frame = +3
Query: 21 LPKDIIFLISQRLDVELDLIRFRSVCSTWRS 51
LP +++ I RL V+ L++FR VC +W S
Sbjct: 264 LPDELVVEILSRLPVK-SLLQFRCVCKSWMS 353
>TC225285 similar to UP|Q9ZS51 (Q9ZS51) PEROXISOMAL MEMBRANE PROTEIN PMP22
(Pmp22 peroxisomal membrane protein) (At4g04470),
partial (88%)
Length = 834
Score = 28.1 bits (61), Expect = 7.3
Identities = 19/46 (41%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +1
Query: 290 ELLLLDIHQTFFQ-FSIKFFKLDEMEKKWVKLKKLGGRVLFVGSGC 334
ELL+LD TFF + IKF K E++K W + L L +G+ C
Sbjct: 256 ELLILDPLDTFFI*YWIKFSKEREIQKLWPRRF*LNS*HLILGTIC 393
>BG404995 similar to GP|22831068|dbj OJ1131_E05.32 {Oryza sativa (japonica
cultivar-group)}, partial (8%)
Length = 394
Score = 27.7 bits (60), Expect = 9.6
Identities = 13/31 (41%), Positives = 20/31 (63%)
Frame = +1
Query: 21 LPKDIIFLISQRLDVELDLIRFRSVCSTWRS 51
LP++++ I RL V L+RFRS +W+S
Sbjct: 40 LPREVLTEILSRLPVR-SLLRFRSTSKSWKS 129
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.138 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,164,074
Number of Sequences: 63676
Number of extensions: 339370
Number of successful extensions: 1667
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 1650
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1664
length of query: 400
length of database: 12,639,632
effective HSP length: 99
effective length of query: 301
effective length of database: 6,335,708
effective search space: 1907048108
effective search space used: 1907048108
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
Medicago: description of AC122162.12