BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122162.11 - phase: 0
(208 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF325009 67 6e-12
BU547704 65 3e-11
TC231475 similar to UP|Q8LFC0 (Q8LFC0) NAD+ dependent isocitrate... 28 2.3
BG404941 28 2.3
TC214174 28 2.3
TC230852 similar to UP|Q6UY78 (Q6UY78) YDA, partial (3%) 28 3.0
TC230574 28 3.0
TC229506 similar to UP|Q9SXX9 (Q9SXX9) FAS1 (Fragment), partial ... 27 5.1
TC231742 similar to UP|Q7XJ53 (Q7XJ53) At3g06270, partial (47%) 27 5.1
TC214090 27 6.6
TC220960 weakly similar to UP|O80960 (O80960) Nodulin-like prote... 27 6.6
TC214213 homologue to UP|PRSA_BRACM (O23894) 26S protease regula... 27 6.6
TC234046 homologue to UP|Q8LJR5 (Q8LJR5) Small GTP-binding prote... 27 8.6
TC214304 similar to UP|O23298 (O23298) Elicitor like protein (AT... 27 8.6
TC225141 type 2 metallothionein [Glycine max] 27 8.6
>BF325009
Length = 382
Score = 67.0 bits (162), Expect = 6e-12
Identities = 44/111 (39%), Positives = 60/111 (53%), Gaps = 11/111 (9%)
Frame = +2
Query: 43 ICLFKGRIYAVNQSGETVTVGP-DSSVELAAQPLDPGIPGLNKMLVESEGRLLLLAINEM 101
+C+FKG +Y + +G TV V P D + LAA+P+ G K LVESEG LLL+ +
Sbjct: 2 VCVFKGNLYGADSNGRTVRVRPDDGGLTLAAEPV---FGGDKKFLVESEGALLLVDMYLS 172
Query: 102 FYS-----FSIDFFKLDEKEKKWVRLMDF-----DEKEKKWVKLRHFGDRI 142
+YS F DF + D W R + F DE+ KKWV+L DR+
Sbjct: 173 YYSCTQGLFHEDFDEEDVAGMGWERTVKFDVFRLDEEGKKWVELTDLRDRV 325
>BU547704
Length = 600
Score = 64.7 bits (156), Expect = 3e-11
Identities = 39/100 (39%), Positives = 57/100 (57%), Gaps = 13/100 (13%)
Frame = -2
Query: 119 WVRLMDFD-----EKEKKWVKLRHFGDRIFFIGRGCSFSASASDLCIPKGNCVVFIDETV 173
W R + FD E+ KKWV+L G+R+ F+G C+FSASA DL + +GNCV D+ +
Sbjct: 572 WERTVKFDVFXLDEEGKKWVELTDLGERVLFLGDDCAFSASAKDLNLGRGNCVAXRDDGL 393
Query: 174 LW---IDNKRVFHLDQNQLS----CGAKYLNLFL-PPKWI 205
+ ++ VF LD ++S C A + LF PP W+
Sbjct: 392 GFNRVLNGMGVFRLDDGKISPLSEC-AGFSELFCPPPDWV 276
>TC231475 similar to UP|Q8LFC0 (Q8LFC0) NAD+ dependent isocitrate
dehydrogenase subunit 1, partial (24%)
Length = 589
Score = 28.5 bits (62), Expect = 2.3
Identities = 14/46 (30%), Positives = 23/46 (49%)
Frame = -1
Query: 117 KKWVRLMDFDEKEKKWVKLRHFGDRIFFIGRGCSFSASASDLCIPK 162
+K+ RL+ F E K + L+H +G S SD+C+P+
Sbjct: 319 QKYTRLISFGR*ENK*ISLKHLSLHQQPLGWCLSLQGPLSDICLPE 182
>BG404941
Length = 379
Score = 28.5 bits (62), Expect = 2.3
Identities = 19/67 (28%), Positives = 31/67 (45%)
Frame = +2
Query: 50 IYAVNQSGETVTVGPDSSVELAAQPLDPGIPGLNKMLVESEGRLLLLAINEMFYSFSIDF 109
IY Q + + S V+ + L+P +PGL + + S R LL+++ +D
Sbjct: 86 IYTELQYSDLSSPSTSSMVDSIMEALEPTVPGLLAVPIASNLRSHLLSLSRKLALLDLDT 265
Query: 110 FKLDEKE 116
KL KE
Sbjct: 266 RKLVLKE 286
>TC214174
Length = 822
Score = 28.5 bits (62), Expect = 2.3
Identities = 23/85 (27%), Positives = 37/85 (43%)
Frame = +2
Query: 78 GIPGLNKMLVESEGRLLLLAINEMFYSFSIDFFKLDEKEKKWVRLMDFDEKEKKWVKLRH 137
G+ G+ +L S G LLL + DF+ D ++K++++L K +
Sbjct: 308 GLGGILFILGSSFGAFLLLLHQMIATPILYDFYYYDSEDKEFIQLF-----IKFTQNMAL 472
Query: 138 FGDRIFFIGRGCSFSASASDLCIPK 162
FG +FFIG S +PK
Sbjct: 473 FGALLFFIGMKNSIPRRQPKKKVPK 547
>TC230852 similar to UP|Q6UY78 (Q6UY78) YDA, partial (3%)
Length = 622
Score = 28.1 bits (61), Expect = 3.0
Identities = 13/42 (30%), Positives = 22/42 (51%)
Frame = -1
Query: 93 LLLLAINEMFYSFSIDFFKLDEKEKKWVRLMDFDEKEKKWVK 134
L+LL +FY++ L + KW+ L + ++ KKW K
Sbjct: 562 LILLCFGSLFYNYF-----LSK*NNKWIILTNIEDLLKKWAK 452
>TC230574
Length = 848
Score = 28.1 bits (61), Expect = 3.0
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +3
Query: 118 KWVRLMDFDEKEKKWVKLRHFGDRI 142
+WV+ DFDE KK ++R+ DR+
Sbjct: 441 EWVKRQDFDELRKKMKEIRNERDRL 515
>TC229506 similar to UP|Q9SXX9 (Q9SXX9) FAS1 (Fragment), partial (36%)
Length = 923
Score = 27.3 bits (59), Expect = 5.1
Identities = 18/55 (32%), Positives = 27/55 (48%)
Frame = +1
Query: 33 ISKRSYEYGDICLFKGRIYAVNQSGETVTVGPDSSVELAAQPLDPGIPGLNKMLV 87
+ K E ++CL G+ A SG V V PDS + + + G+NK+LV
Sbjct: 244 VDKMQDEDQEVCLSTGKSGASPISG--VAVIPDSDLPIIVTTIQSCSQGMNKVLV 402
>TC231742 similar to UP|Q7XJ53 (Q7XJ53) At3g06270, partial (47%)
Length = 529
Score = 27.3 bits (59), Expect = 5.1
Identities = 12/24 (50%), Positives = 15/24 (62%), Gaps = 1/24 (4%)
Frame = -2
Query: 182 FHLDQNQLSCGAKYLNL-FLPPKW 204
FHL Q C K+ +L F+PPKW
Sbjct: 108 FHLINTQHPCPTKFHSLIFIPPKW 37
>TC214090
Length = 1027
Score = 26.9 bits (58), Expect = 6.6
Identities = 20/69 (28%), Positives = 32/69 (45%)
Frame = +1
Query: 78 GIPGLNKMLVESEGRLLLLAINEMFYSFSIDFFKLDEKEKKWVRLMDFDEKEKKWVKLRH 137
G+ G+ + S G LLLL + DF+ D ++K++ +L K +
Sbjct: 307 GLGGVLFIFGSSFGALLLLLHQLIATPIHYDFYNYDSEDKEFTQLF-----IKFTQNMAL 471
Query: 138 FGDRIFFIG 146
FG +FFIG
Sbjct: 472 FGALLFFIG 498
>TC220960 weakly similar to UP|O80960 (O80960) Nodulin-like protein
(At2g39210/T16B24.15), partial (10%)
Length = 490
Score = 26.9 bits (58), Expect = 6.6
Identities = 15/37 (40%), Positives = 18/37 (48%)
Frame = -3
Query: 135 LRHFGDRIFFIGRGCSFSASASDLCIPKGNCVVFIDE 171
LRHF F RGCS S + KG+C + DE
Sbjct: 230 LRHFS----FCHRGCSIPVSLVYVTFVKGSCPSYQDE 132
>TC214213 homologue to UP|PRSA_BRACM (O23894) 26S protease regulatory subunit
6A homolog (TAT-binding protein homolog 1) (TBP-1),
partial (64%)
Length = 1086
Score = 26.9 bits (58), Expect = 6.6
Identities = 12/28 (42%), Positives = 14/28 (49%)
Frame = +1
Query: 144 FIGRGCSFSASASDLCIPKGNCVVFIDE 171
FIG G A L K C++FIDE
Sbjct: 271 FIGDGAKLVRDAFQLAKEKSPCIIFIDE 354
>TC234046 homologue to UP|Q8LJR5 (Q8LJR5) Small GTP-binding protein, partial
(41%)
Length = 485
Score = 26.6 bits (57), Expect = 8.6
Identities = 15/43 (34%), Positives = 24/43 (54%)
Frame = +1
Query: 26 LDEQWKLISKRSYEYGDICLFKGRIYAVNQSGETVTVGPDSSV 68
L + ++++SKR+ E G+ AV G+T+ V DSSV
Sbjct: 124 LSQIYRIVSKRAVEAGN----NASSSAVPSKGQTINVKDDSSV 240
>TC214304 similar to UP|O23298 (O23298) Elicitor like protein
(AT4g14420/dl3250c), partial (50%)
Length = 739
Score = 26.6 bits (57), Expect = 8.6
Identities = 20/69 (28%), Positives = 32/69 (45%)
Frame = +3
Query: 78 GIPGLNKMLVESEGRLLLLAINEMFYSFSIDFFKLDEKEKKWVRLMDFDEKEKKWVKLRH 137
G+ G+ + S G LLLL + DF+ D ++K++ +L K +
Sbjct: 150 GLGGVLFIFGSSFGALLLLLHQLIATPIHYDFYNYDSEDKEFTQLF-----IKFTQNMAL 314
Query: 138 FGDRIFFIG 146
FG +FFIG
Sbjct: 315 FGAVLFFIG 341
>TC225141 type 2 metallothionein [Glycine max]
Length = 912
Score = 26.6 bits (57), Expect = 8.6
Identities = 17/55 (30%), Positives = 28/55 (50%)
Frame = +2
Query: 99 NEMFYSFSIDFFKLDEKEKKWVRLMDFDEKEKKWVKLRHFGDRIFFIGRGCSFSA 153
N + S I + EK+KK +K+K ++RH G+ +FF G+ C +A
Sbjct: 653 NLNYESLLIILNTITEKKKK-------KKKKKPSCRIRHEGNVVFFHGQRCEGAA 796
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.325 0.144 0.457
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,547,623
Number of Sequences: 63676
Number of extensions: 172832
Number of successful extensions: 951
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 936
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 949
length of query: 208
length of database: 12,639,632
effective HSP length: 93
effective length of query: 115
effective length of database: 6,717,764
effective search space: 772542860
effective search space used: 772542860
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC122162.11