BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122162.1 + phase: 0 /pseudo/partial
(517 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC232469 similar to GB|AAP21178.1|30102520|BT006370 At3g08040/T8... 302 2e-82
TC209444 similar to GB|AAP21178.1|30102520|BT006370 At3g08040/T8... 257 1e-68
BM893242 149 4e-36
TC223912 similar to GB|AAP21178.1|30102520|BT006370 At3g08040/T8... 113 1e-28
TC222491 similar to GB|AAP21178.1|30102520|BT006370 At3g08040/T8... 96 5e-20
TC234001 weakly similar to UP|Q8PS88 (Q8PS88) DinF protein, part... 95 7e-20
TC224312 93 3e-19
TC223886 85 7e-17
AI507870 74 2e-13
TC222888 68 1e-11
AW100544 65 1e-10
TC217005 31 1.2
TC225744 homologue to UP|Q9SPB8 (Q9SPB8) Malate dehydrogenase, c... 30 3.4
BG043416 weakly similar to GP|15081773|gb At1g61890/F8K4_9 {Arab... 29 4.5
TC222052 29 5.8
TC227415 similar to UP|Q9LVD9 (Q9LVD9) Gb|AAC28507.1, partial (89%) 28 9.9
TC229021 28 9.9
BM309094 28 9.9
TC205716 similar to UP|Q8W0Z0 (Q8W0Z0) AT5g44100/MLN1_2, partial... 28 9.9
>TC232469 similar to GB|AAP21178.1|30102520|BT006370 At3g08040/T8G24.8
{Arabidopsis thaliana;} , partial (33%)
Length = 822
Score = 302 bits (774), Expect = 2e-82
Identities = 152/173 (87%), Positives = 161/173 (92%)
Frame = +2
Query: 345 SASLAARLGPIPMAAFQTCLQVWMTSSLLADGLAVAIQAILACSFAEKDYNKVTTAATRT 404
+ASLAAR GPIPMAAFQTCLQVW+TSSLLADGLAVA+QAILACSFAEKDY KV AATRT
Sbjct: 2 AASLAARFGPIPMAAFQTCLQVWLTSSLLADGLAVAVQAILACSFAEKDYEKVLVAATRT 181
Query: 405 LQMSFVLGVGLSLVVGGGLYFGAGVFSKNVAVIHLIRLGLPFVAATQPINSLAFVFDGVN 464
LQMSFVLGVGLS VG GLYFGAG+FSK+V V+HLIR+GLPFVAATQPINSLAFVFDGVN
Sbjct: 182 LQMSFVLGVGLSFAVGVGLYFGAGIFSKSVLVVHLIRIGLPFVAATQPINSLAFVFDGVN 361
Query: 465 YGASDFAYSAYSLVMVSIASVTSLFFLYKSKGFIGIWIALTIYMSLRMFAGVW 517
YGASDFAYSAYSLV VS+ASV SLF L KSKGF+GIWIALTIYMSLRMFAGVW
Sbjct: 362 YGASDFAYSAYSLVTVSLASVASLFLLSKSKGFVGIWIALTIYMSLRMFAGVW 520
>TC209444 similar to GB|AAP21178.1|30102520|BT006370 At3g08040/T8G24.8
{Arabidopsis thaliana;} , partial (37%)
Length = 931
Score = 257 bits (656), Expect = 1e-68
Identities = 127/189 (67%), Positives = 156/189 (82%)
Frame = +3
Query: 327 NGGLLLARVIAVTFCVTLSASLAARLGPIPMAAFQTCLQVWMTSSLLADGLAVAIQAILA 386
NG LLL RVIAVTFCVTL+ASLAAR GP MAAFQ CLQVW+ SLLADGLAVA QAILA
Sbjct: 3 NGFLLLMRVIAVTFCVTLAASLAARQGPTSMAAFQVCLQVWLAVSLLADGLAVAGQAILA 182
Query: 387 CSFAEKDYNKVTTAATRTLQMSFVLGVGLSLVVGGGLYFGAGVFSKNVAVIHLIRLGLPF 446
+FA KD+N+ T A+R LQM VLG+ L+ ++G GL+FGA +F+++ V+HLI++G+PF
Sbjct: 183 GAFANKDFNRATATASRVLQMGLVLGLALAFILGTGLHFGAKIFTQDANVLHLIQIGIPF 362
Query: 447 VAATQPINSLAFVFDGVNYGASDFAYSAYSLVMVSIASVTSLFFLYKSKGFIGIWIALTI 506
+A TQP+NSLAFVFDGVN+GASDFAYSA+S+V+V+I S+ SL L + GFIGIW+ALTI
Sbjct: 363 IAVTQPLNSLAFVFDGVNFGASDFAYSAFSMVVVAILSIISLLILSSAGGFIGIWVALTI 542
Query: 507 YMSLRMFAG 515
YM LR FAG
Sbjct: 543 YMGLRAFAG 569
>BM893242
Length = 421
Score = 149 bits (375), Expect = 4e-36
Identities = 78/128 (60%), Positives = 96/128 (74%)
Frame = +3
Query: 155 NISKSSIVTNSSNKSESKPIRKKRHIASASTALLFGTVLGLIQAATLIFAAKPLLGAMGL 214
N+ S V S N ++ + RK+RHI SAS+A+ G +LGLIQA LI AAKPLL MG+
Sbjct: 42 NVHNSDFVGESFNIAKEE--RKRRHIPSASSAIFIGGILGLIQAIFLISAAKPLLNFMGV 215
Query: 215 KYDSPMLVPAVKYLRLRALGAPAVLLSLAMQGIFRGFKDTTTPLYVIVSGYALNVAMDPL 274
DSPML PA +YL+LR+LGAPAVLLSLAMQG+FRGFKDT TPLY V+G N+A+DPL
Sbjct: 216 TSDSPMLHPAKQYLKLRSLGAPAVLLSLAMQGVFRGFKDTKTPLYATVAGDVTNIALDPL 395
Query: 275 LIFYFKLG 282
+F F+LG
Sbjct: 396 FMFVFRLG 419
>TC223912 similar to GB|AAP21178.1|30102520|BT006370 At3g08040/T8G24.8
{Arabidopsis thaliana;} , partial (16%)
Length = 755
Score = 113 bits (282), Expect(2) = 1e-28
Identities = 52/72 (72%), Positives = 63/72 (87%)
Frame = +3
Query: 446 FVAATQPINSLAFVFDGVNYGASDFAYSAYSLVMVSIASVTSLFFLYKSKGFIGIWIALT 505
+VAATQPIN+LAFVFDGVNYGASDF YSAYS++MV++ S+ SL+ L S GF GIWIAL+
Sbjct: 249 YVAATQPINALAFVFDGVNYGASDFTYSAYSMIMVALVSILSLYTLSSSLGFTGIWIALS 428
Query: 506 IYMSLRMFAGVW 517
IYM+LR+FAG W
Sbjct: 429 IYMTLRIFAGFW 464
Score = 31.6 bits (70), Expect(2) = 1e-28
Identities = 15/43 (34%), Positives = 28/43 (64%)
Frame = +2
Query: 403 RTLQMSFVLGVGLSLVVGGGLYFGAGVFSKNVAVIHLIRLGLP 445
R LQ+ +LG+ LS+++ L F + +F+ + V+ LI +G+P
Sbjct: 2 RVLQLGLILGLVLSVLLLSLLPFASRLFTNDNNVLQLISIGIP 130
>TC222491 similar to GB|AAP21178.1|30102520|BT006370 At3g08040/T8G24.8
{Arabidopsis thaliana;} , partial (11%)
Length = 429
Score = 95.5 bits (236), Expect = 5e-20
Identities = 43/57 (75%), Positives = 51/57 (89%)
Frame = +3
Query: 461 DGVNYGASDFAYSAYSLVMVSIASVTSLFFLYKSKGFIGIWIALTIYMSLRMFAGVW 517
DGVNYGASDFAYSAYSLV+VS+ SV + F LY++K F+GIWIALTIYM+LRM AG+W
Sbjct: 3 DGVNYGASDFAYSAYSLVLVSLVSVATEFILYRTKHFVGIWIALTIYMTLRMLAGIW 173
>TC234001 weakly similar to UP|Q8PS88 (Q8PS88) DinF protein, partial (6%)
Length = 766
Score = 95.1 bits (235), Expect = 7e-20
Identities = 54/101 (53%), Positives = 63/101 (61%), Gaps = 30/101 (29%)
Frame = +1
Query: 258 LYVIVSGYALNVAMDPLLIFYFKLGIRGAAISHVLSQ----------------------- 294
L+ VSGYALNV +DP+LIFY KLGI+GAAISHVLSQ
Sbjct: 280 LFCSVSGYALNVILDPVLIFYCKLGIKGAAISHVLSQ*VLPLSYSSNVQTFHLAKSELTK 459
Query: 295 -------YIMATLLLFILMKKVDLLPPSMKDLQIFRFLKNG 328
Y+MA L+ IL +KVDL+PPS+KDLQIFRFLKNG
Sbjct: 460 W*SFLIRYLMALALMVILTRKVDLVPPSIKDLQIFRFLKNG 582
>TC224312
Length = 806
Score = 93.2 bits (230), Expect = 3e-19
Identities = 49/91 (53%), Positives = 63/91 (68%)
Frame = +2
Query: 26 KMDSLAKEILGIAFPSALAVAADPIASLIDTAFIGHLGPVELAAAGVSIAVFNQASRITI 85
K D L EI+ IA P+ALA+AADPIASLIDTAF+GH+G VELAA GVS + FN S+
Sbjct: 263 KFDELGMEIVLIALPAALALAADPIASLIDTAFVGHIGAVELAAVGVSASGFNLVSKAFN 442
Query: 86 FPLVSITTSFVAEEDTMDRINTKAAEKQFNE 116
PL+++T SFVAEE + R ++ N+
Sbjct: 443 VPLLNVTASFVAEEQALIRKEEESIPSDKND 535
>TC223886
Length = 653
Score = 85.1 bits (209), Expect = 7e-17
Identities = 39/106 (36%), Positives = 68/106 (63%)
Frame = +3
Query: 410 VLGVGLSLVVGGGLYFGAGVFSKNVAVIHLIRLGLPFVAATQPINSLAFVFDGVNYGASD 469
V+G+ L+ ++G A +F+++ V+ +++ FV+A+QP N+LA++FDG++YG SD
Sbjct: 6 VMGICLTAILGASFGSLATIFTQDXEVLQVVKTLALFVSASQPFNALAYIFDGLHYGVSD 185
Query: 470 FAYSAYSLVMVSIASVTSLFFLYKSKGFIGIWIALTIYMSLRMFAG 515
F Y+A+S++ V S L F G G+W+ L ++M+LR AG
Sbjct: 186 FRYAAFSMMFVGAVSSAFLVFAPPLFGLQGVWLGLVLFMALRAAAG 323
>AI507870
Length = 205
Score = 73.9 bits (180), Expect = 2e-13
Identities = 35/66 (53%), Positives = 49/66 (74%)
Frame = +2
Query: 269 VAMDPLLIFYFKLGIRGAAISHVLSQYIMATLLLFILMKKVDLLPPSMKDLQIFRFLKNG 328
V + PLL++YF+LG+ GAAIS V+SQYI L+++ L K+ +LLPP M DLQ ++K+G
Sbjct: 8 VFLFPLLMYYFRLGVTGAAISTVISQYIGTMLMIWCLNKRAELLPPKMGDLQFGSYIKSG 187
Query: 329 GLLLAR 334
G LL R
Sbjct: 188 GFLLGR 205
>TC222888
Length = 1066
Score = 67.8 bits (164), Expect = 1e-11
Identities = 33/72 (45%), Positives = 48/72 (65%), Gaps = 4/72 (5%)
Frame = +3
Query: 411 LGVGLSLVVGGGLYFGAGVFSK----NVAVIHLIRLGLPFVAATQPINSLAFVFDGVNYG 466
+G+G + + L+FG G FS + V+ + R G+ FVA +QP+N+LAFV DG+ YG
Sbjct: 282 IGLGAGITLSMILFFGFGAFSSLFSTDSEVLDVARSGIWFVAGSQPVNALAFVIDGIYYG 461
Query: 467 ASDFAYSAYSLV 478
SDF Y+AYS+V
Sbjct: 462 VSDFGYAAYSMV 497
>AW100544
Length = 285
Score = 64.7 bits (156), Expect = 1e-10
Identities = 34/75 (45%), Positives = 45/75 (59%)
Frame = +2
Query: 187 LLFGTVLGLIQAATLIFAAKPLLGAMGLKYDSPMLVPAVKYLRLRALGAPAVLLSLAMQG 246
L LG+ + L + L+ M + DSPM PA ++L LRA G PA++L+LA QG
Sbjct: 2 LALAATLGMAETVVLTLGSGILMNIMAV*-DSPMRGPAEQFLTLRAFGTPAIVLALAAQG 178
Query: 247 IFRGFKDTTTPLYVI 261
FRGF DT TPLY +
Sbjct: 179 TFRGFLDTKTPLYAV 223
>TC217005
Length = 1440
Score = 31.2 bits (69), Expect = 1.2
Identities = 11/38 (28%), Positives = 23/38 (59%)
Frame = +1
Query: 104 RINTKAAEKQFNESGKAKSNEVMPDDHLLQDIEAGATK 141
++ ++++ + S K SNE+ P+ +QD+E G T+
Sbjct: 868 KVGSQSSGNDHSSSNKRSSNEIFPEPLEIQDVETGVTR 981
>TC225744 homologue to UP|Q9SPB8 (Q9SPB8) Malate dehydrogenase, complete
Length = 1476
Score = 29.6 bits (65), Expect = 3.4
Identities = 25/87 (28%), Positives = 38/87 (42%)
Frame = +3
Query: 61 HLGPVELAAAGVSIAVFNQASRITIFPLVSITTSFVAEEDTMDRINTKAAEKQFNESGKA 120
+ G + AGV++ V + ITI PL S T +D + + TK + E +A
Sbjct: 657 YAGKANVPVAGVNVPVVGGHAGITILPLFSQATPKANLDDDVIKALTKRTQDGGTEVVEA 836
Query: 121 KSNEVMPDDHLLQDIEAGATKQDSTLK 147
K+ + L AGA D+ LK
Sbjct: 837 KAGK---GSATLSMAYAGALFADACLK 908
>BG043416 weakly similar to GP|15081773|gb At1g61890/F8K4_9 {Arabidopsis
thaliana}, partial (20%)
Length = 386
Score = 29.3 bits (64), Expect = 4.5
Identities = 14/36 (38%), Positives = 22/36 (60%)
Frame = +3
Query: 37 IAFPSALAVAADPIASLIDTAFIGHLGPVELAAAGV 72
+A P+ L + + S + AF GHLG ++LAAA +
Sbjct: 174 LAAPAILVYVFNNLMSNVTRAFAGHLGNLKLAAANL 281
>TC222052
Length = 832
Score = 28.9 bits (63), Expect = 5.8
Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = +2
Query: 402 TRTLQMSFVLGVGLSLVVGGGLYFGAGVFSKNVAVIHLIRLGLPFVAATQ-PIN 454
T T+ +F L + ++ +GG FGA +F + L +G V ATQ P+N
Sbjct: 50 TNTISNAFKL-LSIATFIGGACCFGAFLFKSQYGFLALFAVGELLVFATQGPVN 208
>TC227415 similar to UP|Q9LVD9 (Q9LVD9) Gb|AAC28507.1, partial (89%)
Length = 1748
Score = 28.1 bits (61), Expect = 9.9
Identities = 14/41 (34%), Positives = 23/41 (55%)
Frame = +1
Query: 32 KEILGIAFPSALAVAADPIASLIDTAFIGHLGPVELAAAGV 72
K + +A P+ + + + S+ F GHLG +ELAAA +
Sbjct: 265 KLLFFLAAPAVIVYLINYLMSMSTQIFSGHLGNLELAAASL 387
>TC229021
Length = 1033
Score = 28.1 bits (61), Expect = 9.9
Identities = 17/53 (32%), Positives = 24/53 (45%)
Frame = -3
Query: 136 EAGATKQDSTLKNGDDANSNISKSSIVTNSSNKSESKPIRKKRHIASASTALL 188
++ TKQ LK+ N+N S T S +K I RHI + +LL
Sbjct: 902 QSSLTKQTKKLKDESIGNTNTILESFCTKGLGSSCNKTIHILRHIIPSRPSLL 744
>BM309094
Length = 192
Score = 28.1 bits (61), Expect = 9.9
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = +3
Query: 289 SHVLSQYIMATLLLFILMKKVDLLPPSMKDLQIFRF 324
SHV +Q I +L FIL+ + +P +D+ F
Sbjct: 51 SHVFAQAIKYLILKFILLNVISCIPELFRDISYINF 158
>TC205716 similar to UP|Q8W0Z0 (Q8W0Z0) AT5g44100/MLN1_2, partial (16%)
Length = 986
Score = 28.1 bits (61), Expect = 9.9
Identities = 15/61 (24%), Positives = 33/61 (53%)
Frame = +3
Query: 115 NESGKAKSNEVMPDDHLLQDIEAGATKQDSTLKNGDDANSNISKSSIVTNSSNKSESKPI 174
+E + +S++ +P +D++A + + S+ +NG + + SS ++S SES+
Sbjct: 195 SEHSRHRSSDDVPSS---KDVQADSERPRSSSRNGSSSKKPVLSSSRPSSSGEPSESRTS 365
Query: 175 R 175
R
Sbjct: 366 R 368
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.327 0.139 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,187,920
Number of Sequences: 63676
Number of extensions: 224519
Number of successful extensions: 1457
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 1443
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1455
length of query: 517
length of database: 12,639,632
effective HSP length: 101
effective length of query: 416
effective length of database: 6,208,356
effective search space: 2582676096
effective search space used: 2582676096
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC122162.1