BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122160.5 - phase: 0
(433 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC229793 homologue to UP|FD6C_SOYBN (P48628) Omega-6 fatty acid ... 749 0.0
TC208804 UP|Q9FXK9 (Q9FXK9) Microsomal omega-3 fatty acid desatu... 134 1e-31
TC204934 similar to UP|Q8H1X9 (Q8H1X9) Omega-3 fatty acid desatu... 130 1e-30
TC207696 UP|Q84X84 (Q84X84) Microsomal omega-3-fatty acid desatu... 115 3e-26
TC229997 UP|Q84X83 (Q84X83) Microsomal omega-3-fatty acid desatu... 113 2e-25
TC214992 homologue to UP|FD62_SOYBN (P48631) Omega-6 fatty acid ... 99 4e-21
TC204860 homologue to UP|FD61_SOYBN (P48630) Omega-6 fatty acid ... 90 2e-18
TC204936 homologue to UP|FD3C_SOYBN (P48621) Omega-3 fatty acid ... 82 6e-16
TC219346 similar to UP|FD62_SOYBN (P48631) Omega-6 fatty acid de... 68 7e-12
TC204861 homologue to UP|FD61_SOYBN (P48630) Omega-6 fatty acid ... 66 3e-11
BM121495 similar to SP|P48631|FD62 Omega-6 fatty acid desaturase... 65 5e-11
TC204932 homologue to UP|FD3C_SOYBN (P48621) Omega-3 fatty acid ... 63 2e-10
BG042278 56 3e-08
BG650512 54 2e-07
BF066409 similar to GP|2613051|gb|A omega-6 desaturase {Arachis ... 47 1e-05
BE800553 GP|27902573|g microsomal omega-3-fatty acid desaturase ... 42 1e-04
TC205449 homologue to UP|FD62_SOYBN (P48631) Omega-6 fatty acid ... 42 4e-04
TC227314 similar to UP|Q9ZRP8 (Q9ZRP8) Delta-8 sphingolipid desa... 39 0.005
TC204935 similar to UP|P93350 (P93350) Plastid omega-3 fatty aci... 37 0.023
BI426946 similar to GP|12597245|dbj cytochrome b561 {Arabidopsis... 33 0.25
>TC229793 homologue to UP|FD6C_SOYBN (P48628) Omega-6 fatty acid desaturase,
chloroplast precursor , complete
Length = 1719
Score = 749 bits (1934), Expect = 0.0
Identities = 345/414 (83%), Positives = 376/414 (90%)
Frame = +3
Query: 3 SFQRPILNLRTNISAPYSPGLFNLKGDGLIHKGFRHQSQKHLTPRNKVTVIRAVAIPVEP 62
S+Q+P+L R +I+A YSPG+F+L +GLI K FR Q ++ RNKVTVI AVAIPV+P
Sbjct: 132 SYQKPVL--RRDIAARYSPGIFSLNSNGLIQKRFRRQ--RNFVTRNKVTVIHAVAIPVQP 299
Query: 63 APVESAEYRKQLAERYGFEQIGEPLPDNVTLKDVITSLPKKVFEIDDVKAWKSVLISATS 122
APVESAEYRKQLAE YGF Q+GEPLPD+VTLKDVI SLPK+VFEIDDVKAWKSVLIS TS
Sbjct: 300 APVESAEYRKQLAEDYGFRQVGEPLPDDVTLKDVINSLPKEVFEIDDVKAWKSVLISVTS 479
Query: 123 YALGLLMISKAPWYLLPLAWAWTGTAVTGFFVIGHDCAHKSFSTNKLVEDIVGTLAFLPL 182
YALGL MISKAPWYLLPLAW WTGTA+TGFFVIGHDCAH+SFS+NKLVEDIVGTLAF+PL
Sbjct: 480 YALGLFMISKAPWYLLPLAWVWTGTAITGFFVIGHDCAHRSFSSNKLVEDIVGTLAFMPL 659
Query: 183 IYPYEPWRFKHDKHHAKTNMLKEDTAWQPVWQDEFESSPFLRKAIIYGYGPFRCWMSIAH 242
IYPYEPWRFKHD+HHAKTNML+EDTAW PVW+DEFES+P LRKAIIYGYGPFRCWMSIAH
Sbjct: 660 IYPYEPWRFKHDRHHAKTNMLREDTAWHPVWKDEFESTPLLRKAIIYGYGPFRCWMSIAH 839
Query: 243 WLVSHFDLKTFRPNEIKRVKISLACVFAFIAIGWPLIIYKTGIMGWIKFWLMPWLGYHFW 302
WL+ HFDLK FRP+E+ RVKISLACVFAFIAIGWPLIIYKTGIMGWIKFWLMPWLGYHFW
Sbjct: 840 WLMWHFDLKKFRPSEVPRVKISLACVFAFIAIGWPLIIYKTGIMGWIKFWLMPWLGYHFW 1019
Query: 303 MSTFTMVHHTAPHIPFKNSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPKI 362
MSTFTMVHHTAPHIPFK SEEWN AQAQLNGTVHC YP+WIEILCHDINVHIPHHISP+I
Sbjct: 1020MSTFTMVHHTAPHIPFKYSEEWNPAQAQLNGTVHCDYPKWIEILCHDINVHIPHHISPRI 1199
Query: 363 PSYNLRAAHKSIQENWGKYLNEASWNWRLMKTIMTVCHVYDKQQNYVSFDEVAP 416
PSYNLRAAHKS+QENWG+YLNEASWNWRLMKTIMTVC VYDK++N P
Sbjct: 1200PSYNLRAAHKSLQENWGQYLNEASWNWRLMKTIMTVCQVYDKEKNLCCLRRTCP 1361
Score = 46.6 bits (109), Expect = 2e-05
Identities = 19/30 (63%), Positives = 27/30 (89%)
Frame = +1
Query: 404 KQQNYVSFDEVAPKESRPIRFLKKVMPDYA 433
+++ YV+FDE+AP++SRPI FLK+ MPDYA
Sbjct: 1324 RRKIYVAFDELAPEDSRPITFLKETMPDYA 1413
>TC208804 UP|Q9FXK9 (Q9FXK9) Microsomal omega-3 fatty acid desaturase,
complete
Length = 1407
Score = 134 bits (336), Expect = 1e-31
Identities = 89/312 (28%), Positives = 146/312 (46%), Gaps = 15/312 (4%)
Frame = +2
Query: 88 PDNVTLKDVITSLPKKVFEIDDVKAWKSVLISATSY-ALGLLMISKAPWYLLPLAWAWTG 146
P + ++ ++PK +E + +++ VL AL I W+ PL W G
Sbjct: 143 PPPFKIANIRAAIPKHCWEKNTLRSLSYVLRDVLVVTALVAAAIGFNSWFFWPLYWPAQG 322
Query: 147 TAVTGFFVIGHDCAHKSFSTNKLVEDIVGTLAFLPLIYPYEPWRFKHDKHHAKTNMLKED 206
T FV+GHDC H SFS + L+ IVG + ++ PY WR H HH +++D
Sbjct: 323 TMFWALFVLGHDCGHGSFSNSPLLNSIVGHILHSSILVPYHGWRISHRTHHQNHGHVEKD 502
Query: 207 TAWQPVWQDEFESSPFLRKAI-------IYGYGPFRCWMSIAHWLVSHFD--LKTFRPNE 257
+W P+ + +++ + + + I+ Y PF W SHF+ F P E
Sbjct: 503 ESWVPLTEKVYKNLDNMTRMMRFTLPFPIFAY-PFYLWSRSPGKEGSHFNPYSNLFSPGE 679
Query: 258 IKRVKISLACVFAFIAIGWPLIIYKTGIMG---WIKFWLMPWLGYHFWMSTFTMVHHTA- 313
+ V S C +++ ++Y + MG +K + +P+L + W+ T +HH
Sbjct: 680 RRDVLTSTLCWGIMLSV----LLYLSLTMGPLFMLKLYGVPYLIFVMWLDFVTYLHHHGY 847
Query: 314 -PHIPFKNSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPKIPSYNLRAAHK 372
+P+ +EW+ + L TV Y WI + HDI H+ HH+ P+IP Y+L A K
Sbjct: 848 KQKLPWYRGQEWSYLRGGLT-TVDRDY-GWINNIHHDIGTHVIHHLFPQIPHYHLIEATK 1021
Query: 373 SIQENWGKYLNE 384
+ + GKY E
Sbjct: 1022AAKAVLGKYYRE 1057
>TC204934 similar to UP|Q8H1X9 (Q8H1X9) Omega-3 fatty acid desaturase,
partial (87%)
Length = 1764
Score = 130 bits (327), Expect = 1e-30
Identities = 96/347 (27%), Positives = 153/347 (43%), Gaps = 18/347 (5%)
Frame = +3
Query: 56 VAIPVEPAPVESAEYRKQLAERYGF-EQIGE---PLPDNVTLKDVITSLPKKVFEIDDVK 111
V+ P++ A E E + G E++ E P L D+ ++PK + D K
Sbjct: 261 VSAPLKFATNEGEEEERTNGANNGVGEEVSEFDPSAPPPFKLADIRAAIPKHCWVKDPWK 440
Query: 112 AWKSVLISA-TSYALGLLMISKAPWYLLPLAWAWTGTAVTGFFVIGHDCAHKSFSTNKLV 170
+ V+ + L W + PL WA GT FV+GHDC H SFS N +
Sbjct: 441 SMSYVVRDVIVVFGLAAAAAYLNNWVVWPLYWAAQGTMFWALFVLGHDCGHGSFSNNPKL 620
Query: 171 EDIVGTLAFLPLIYPYEPWRFKHDKHHAKTNMLKEDTAWQPVWQDEFESSPFLRKAI--- 227
+ G L ++ PY WR H HH ++ D +W P+ + F+S + + +
Sbjct: 621 NSVAGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLPEKIFKSLDNVTRILRFT 800
Query: 228 ----IYGYGPFRCWMSIAHWLVSHF--DLKTFRPNEIKRVKISLAC--VFAFIAIGWPLI 279
+ Y P W SHF D F P+E K V S C A + +G +
Sbjct: 801 LPFPLLAY-PIYLWSRSPGKTGSHFNPDSDLFVPSERKDVITSTICWTAMAALLVGLGFV 977
Query: 280 IYKTGIMGWIKFWLMPWLGYHFWMSTFTMVHHTA--PHIPFKNSEEWNAAQAQLNGTVHC 337
+ G + +K + +P++ + W+ T +HH +P+ EEW+ + L T+
Sbjct: 978 M---GPVQLLKLYGIPYVIFVMWLDLVTYLHHHGHEDKLPWYRGEEWSYLRGGLT-TIDR 1145
Query: 338 AYPQWIEILCHDINVHIPHHISPKIPSYNLRAAHKSIQENWGKYLNE 384
Y WI + HDI H+ HH+ P+IP Y+L A ++ + G+Y E
Sbjct: 1146DY-GWINNIHHDIGTHVIHHLFPQIPHYHLIEATEAAKPVLGQYYRE 1283
>TC207696 UP|Q84X84 (Q84X84) Microsomal omega-3-fatty acid desaturase,
complete
Length = 1294
Score = 115 bits (289), Expect = 3e-26
Identities = 73/264 (27%), Positives = 125/264 (46%), Gaps = 14/264 (5%)
Frame = +2
Query: 135 WYLLPLAWAWTGTAVTGFFVIGHDCAHKSFSTNKLVEDIVGTLAFLPLIYPYEPWRFKHD 194
W L + W GT FV+GHDC H SFS + + +VG + ++ PY WR H
Sbjct: 314 WLLWLIYWPIQGTMFWALFVLGHDCGHGSFSDSPFLNSLVGHILHSSILVPYHGWRISHR 493
Query: 195 KHHAKTNMLKEDTAWQPVWQDEFESSPFLRKAIIYGYGPFRCWMSIAHWLV-------SH 247
HH +++D +W P+ + +++ + + + + PF ++ + SH
Sbjct: 494 THHQNHGHIEKDESWVPLTEKIYKNLDNMTRLVRFTV-PFPLFVYPIYLFSRSPGKEGSH 670
Query: 248 FD--LKTFRPNEIKRVKISLACVFAFIAIGWPLIIYKTGIMG---WIKFWLMPWLGYHFW 302
F+ F P+E K + IS C + ++IY + I +K + +P+ + W
Sbjct: 671 FNPYSNLFPPSERKGIAISTLCWVTMFS----MLIYLSFITSPVLLLKLYGIPYWIFVMW 838
Query: 303 MSTFTMVHHTAPH--IPFKNSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISP 360
+ T +HH H +P+ +EW+ + L TV Y WI + HDI H+ HH+ P
Sbjct: 839 LDFVTYLHHHGHHQKLPWYRGKEWSYLRGGLT-TVDRDY-GWINNIHHDIGTHVIHHLFP 1012
Query: 361 KIPSYNLRAAHKSIQENWGKYLNE 384
+IP Y+L A ++ + G+Y E
Sbjct: 1013QIPHYHLVEATQAAKSVLGEYYRE 1084
>TC229997 UP|Q84X83 (Q84X83) Microsomal omega-3-fatty acid desaturase,
complete
Length = 1321
Score = 113 bits (282), Expect = 2e-25
Identities = 89/331 (26%), Positives = 148/331 (43%), Gaps = 21/331 (6%)
Frame = +1
Query: 75 AERYGFEQIGEPL------PDNVTLKDVITSLPKKVFEIDDVKAWKSVLISATSYA-LGL 127
A G++Q G P + ++ S+PK + + ++ VL A L
Sbjct: 34 AANNGYQQKGSSFDFDPSAPPPFKIAEIRASIPKHCWVKNPWRSLSYVLRDVLVIAALVA 213
Query: 128 LMISKAPWYLLPLAWAWTGTAVTGFFVIGHDCAHKSFSTNKLVEDIVGTLAFLPLIYPYE 187
I W L + GT FV+GHDC H SFS + L+ +VG + ++ PY
Sbjct: 214 AAIHFDNWLLWLIYCPIQGTMFWALFVLGHDCGHGSFSDSPLLNSLVGHILHSSILVPYH 393
Query: 188 PWRFKHDKHHAKTNMLKEDTAWQPVWQDEFESSPFLRKAIIYGYGPFRCWMSIAHWLV-- 245
WR H HH +++D +W P+ + +++ + + I + PF ++ +
Sbjct: 394 GWRISHRTHHQNHGHIEKDESWVPLTEKIYKNLDSMTRLIRFTV-PFPLFVYPIYLFSRS 570
Query: 246 -----SHFD--LKTFRPNEIKRVKISLACVFAFIAIGWPLIIYKTGIMG---WIKFWLMP 295
SHF+ F P+E K + IS C A + L+IY + I +K + +P
Sbjct: 571 PGKEGSHFNPYSNLFPPSERKGIAISTLC----WATMFSLLIYLSFITSPLLVLKLYGIP 738
Query: 296 WLGYHFWMSTFTMVHHTAPH--IPFKNSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVH 353
+ + W+ T +HH H +P+ +EW+ + L TV Y WI + HDI H
Sbjct: 739 YWIFVMWLDFVTYLHHHGHHQKLPWYRGKEWSYLRGGLT-TVDRDY-GWINNIHHDIGTH 912
Query: 354 IPHHISPKIPSYNLRAAHKSIQENWGKYLNE 384
+ HH+ P+IP Y+L A ++ + G Y E
Sbjct: 913 VIHHLFPQIPHYHLVEATQAAKPVLGDYYRE 1005
>TC214992 homologue to UP|FD62_SOYBN (P48631) Omega-6 fatty acid desaturase,
endoplasmic reticulum isozyme 2 , complete
Length = 1556
Score = 99.0 bits (245), Expect = 4e-21
Identities = 81/320 (25%), Positives = 144/320 (44%), Gaps = 16/320 (5%)
Frame = +1
Query: 78 YGFEQIGEPLPDNVTLKDVITSLPKKVFEIDDVKAWKSVLISATSYALGLLMISKAPWYL 137
+ QI + +P + + V S V+++ V AT Y L+ S +
Sbjct: 175 FSLSQIKKVIPPHCFQRSVFRSFSYVVYDLTIAFCLYYV---ATHYFH--LLPSPLSFLA 339
Query: 138 LPLAWAWTGTAVTGFFVIGHDCAHKSFSTNKLVEDIVGTLAFLPLIYPYEPWRFKHDKHH 197
P+ WA G +TG +VI H+C H +FS +L++DIVG + L+ PY W++ H +HH
Sbjct: 340 WPIYWAVQGCILTGVWVIAHECGHHAFSDYQLLDDIVGLVLHSGLLVPYFSWKYSHRRHH 519
Query: 198 AKTNMLKEDTAWQP------VWQDEFESSP---FLRKAIIYGYG-PFRCWMSIA----HW 243
+ T L+ D + P W ++ ++P L A+ G P ++++
Sbjct: 520 SNTGSLERDEVFVPKQKSCIKWYSKYLNNPPGRVLTLAVTLTLGWPLYLALNVSGRPYDR 699
Query: 244 LVSHFDL--KTFRPNEIKRVKISLACVFAFIAIGWPLIIYKTGIMGWIKFWLMPWLGYHF 301
H+D + E ++ IS A V A + G + G+ + + +P L +
Sbjct: 700 FACHYDPYGPIYSDRERLQIYISDAGVLA-VCYGLFRLAMAKGLAWVVCVYGVPLLVVNG 876
Query: 302 WMSTFTMVHHTAPHIPFKNSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPK 361
++ T + HT P +P S EW+ + L TV Y ++ + + H+ HH+
Sbjct: 877 FLVLITFLQHTHPALPHYTSSEWDWLRGAL-ATVDRDYGILNKVFHNITDTHVAHHLFST 1053
Query: 362 IPSYNLRAAHKSIQENWGKY 381
+P Y+ A K+I+ G+Y
Sbjct: 1054MPHYHAMEATKAIKPILGEY 1113
>TC204860 homologue to UP|FD61_SOYBN (P48630) Omega-6 fatty acid desaturase,
endoplasmic reticulum isozyme 1 , complete
Length = 1453
Score = 89.7 bits (221), Expect = 2e-18
Identities = 73/318 (22%), Positives = 142/318 (43%), Gaps = 18/318 (5%)
Frame = +3
Query: 82 QIGEPLPDNVTLKDVITSLPKKVFEIDDVKAWKSVLISATSYALGLLMISKAPWYLL--P 139
Q+ + +P + + ++TS V+++ ++ + AT+Y + P+ L+ P
Sbjct: 201 QLKKAIPPHCFQRSLLTSFSYVVYDL----SFAFIFYIATTY----FHLLPQPFSLIAWP 356
Query: 140 LAWAWTGTAVTGFFVIGHDCAHKSFSTNKLVEDIVGTLAFLPLIYPYEPWRFKHDKHHAK 199
+ W G +TG +VI H+C H +FS + V+D+VG L+ PY W+ H +HH+
Sbjct: 357 IYWVLQGCLLTGVWVIAHECGHHAFSKYQWVDDVVGLTLHSTLLVPYFSWKISHRRHHSN 536
Query: 200 TNMLKEDTAWQP------VWQDEFESSPFLRKAIIY--------GYGPFRCWMSIAHWLV 245
T L D + P W ++ ++P R + Y F
Sbjct: 537 TGSLDRDEVFVPKPKSKVAWYTKYLNNPLGRAVSLLVTLTIGWPMYLAFNVSGRPYDSFA 716
Query: 246 SHFDLKTFRPNEIKRVKISLACVFAFIAIGWPLIIYKT--GIMGWIKFWLMPWLGYHFWM 303
SH+ + +R+ I ++ V A ++ + L T G++ + + +P L + ++
Sbjct: 717 SHYHPYAPIYSNRERLLIYVSDV-ALFSVTYSLYRVATLKGLVWLLCVYGVPLLIVNGFL 893
Query: 304 STFTMVHHTAPHIPFKNSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPKIP 363
T T + HT +P +S EW+ + L T+ Y ++ H + H+ HH+ +P
Sbjct: 894 VTITYLQHTHFALPHYDSSEWDWLKGAL-ATMDRDYGILNKVFHHITDTHVAHHLFSTMP 1070
Query: 364 SYNLRAAHKSIQENWGKY 381
Y+ A +++ G+Y
Sbjct: 1071HYHATEATNAMKPILGEY 1124
>TC204936 homologue to UP|FD3C_SOYBN (P48621) Omega-3 fatty acid desaturase,
chloroplast precursor , partial (46%)
Length = 637
Score = 81.6 bits (200), Expect = 6e-16
Identities = 43/130 (33%), Positives = 61/130 (46%), Gaps = 1/130 (0%)
Frame = +2
Query: 84 GEPLPDNVTLKDVITSLPKKVFEIDDVKAWKSVLISATS-YALGLLMISKAPWYLLPLAW 142
G P P N L D+ ++PK + D +K+ V+ + + L W + PL W
Sbjct: 89 GAPPPFN--LADIRAAIPKHCWVKDPLKSMSYVVRDVIAVFGLASAAAYLNNWLVWPLYW 262
Query: 143 AWTGTAVTGFFVIGHDCAHKSFSTNKLVEDIVGTLAFLPLIYPYEPWRFKHDKHHAKTNM 202
A GT FV+GHDC H SFS N + +VG L ++ PY WR H HH
Sbjct: 263 AAQGTMFWALFVLGHDCGHGSFSNNSKLNSVVGHLLHSSILVPYHGWRISHRTHHQHHGH 442
Query: 203 LKEDTAWQPV 212
+ D +W P+
Sbjct: 443 AENDESWHPL 472
>TC219346 similar to UP|FD62_SOYBN (P48631) Omega-6 fatty acid desaturase,
endoplasmic reticulum isozyme 2 , partial (44%)
Length = 624
Score = 68.2 bits (165), Expect = 7e-12
Identities = 30/84 (35%), Positives = 48/84 (56%)
Frame = +1
Query: 128 LMISKAPWYLLPLAWAWTGTAVTGFFVIGHDCAHKSFSTNKLVEDIVGTLAFLPLIYPYE 187
L+ S + P+ WA G +TG +VI H+C H +FS +L++DIVG + L+ PY
Sbjct: 343 LLPSPLSFLAWPIYWAVQGCILTGVWVIAHECGHHAFSDYQLIDDIVGLVLHSGLLVPYS 522
Query: 188 PWRFKHDKHHAKTNMLKEDTAWQP 211
W++ H H + T L++D + P
Sbjct: 523 AWKYSHRLHLSYTGSLEQDEVFVP 594
>TC204861 homologue to UP|FD61_SOYBN (P48630) Omega-6 fatty acid desaturase,
endoplasmic reticulum isozyme 1 , partial (49%)
Length = 697
Score = 65.9 bits (159), Expect = 3e-11
Identities = 36/127 (28%), Positives = 63/127 (49%), Gaps = 2/127 (1%)
Frame = +1
Query: 82 QIGEPLPDNVTLKDVITSLPKKVFEIDDVKAWKSVLISATSYALGLLMISKAPWYLL--P 139
Q+ + +P + + ++TSL V+++ + + AT+Y + P+ L+ P
Sbjct: 244 QLKKAIPPHCFQRSLLTSLSYVVYDL----SLAFIFYIATTY----FHLLPHPFSLIAWP 399
Query: 140 LAWAWTGTAVTGFFVIGHDCAHKSFSTNKLVEDIVGTLAFLPLIYPYEPWRFKHDKHHAK 199
+ W G +TG +VI H+C H +FS V+D++G L+ PY W+ H HH+
Sbjct: 400 IYWVLQGCILTGVWVIAHECGHHAFSKYPWVDDVMGLTVHSALLVPYFSWKISHRPHHSN 579
Query: 200 TNMLKED 206
T L D
Sbjct: 580 TGSLDRD 600
>BM121495 similar to SP|P48631|FD62 Omega-6 fatty acid desaturase
endoplasmic reticulum isozyme 2 (EC 1.14.19.-).
[Soybean], partial (62%)
Length = 852
Score = 65.5 bits (158), Expect = 5e-11
Identities = 28/87 (32%), Positives = 48/87 (54%), Gaps = 6/87 (6%)
Frame = +1
Query: 141 AWAWTGTAVTGFFVIGHDCAHKSFSTNKLVEDIVGTLAFLPLIYPYEPWRFKHDKHHAKT 200
+WA G +TG +VI H+C H + + +L +DIVG + L+ PY W++ H +HH+ T
Sbjct: 10 SWAVQGCILTGVWVIAHECGHHAXNDYQLXDDIVGLVLHSGLLVPYFSWKYSHRRHHSNT 189
Query: 201 NMLKEDTAWQP------VWQDEFESSP 221
L+ D + P W ++ ++P
Sbjct: 190 GSLERDXXFVPKXXSCIKWYSKYLNNP 270
>TC204932 homologue to UP|FD3C_SOYBN (P48621) Omega-3 fatty acid desaturase,
chloroplast precursor , partial (47%)
Length = 815
Score = 63.2 bits (152), Expect = 2e-10
Identities = 47/146 (32%), Positives = 74/146 (50%), Gaps = 7/146 (4%)
Frame = +1
Query: 246 SHFDLKT--FRPNEIKRVKISLACVFAFIAIGWPLIIYKTGIMGWI---KFWLMPWLGYH 300
SHFD + F PNE K V S AC A + L++ +MG I K + +P++ +
Sbjct: 88 SHFDPSSDLFVPNERKDVITSTACWAAMLG----LLVGLGFVMGPIQLLKLYGVPYVIFV 255
Query: 301 FWMSTFTMVHHTAPH--IPFKNSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHI 358
W+ T +HH +P+ +EW+ + L T+ Y I + HDI H+ HH+
Sbjct: 256 MWLDLVTYLHHHGHEDKLPWYRGKEWSYLRGGLT-TLDRDYGL-INNIHHDIGTHVIHHL 429
Query: 359 SPKIPSYNLRAAHKSIQENWGKYLNE 384
P+IP Y+L A ++ + +GKY E
Sbjct: 430 FPQIPHYHLVEATEAAKPVFGKYYRE 507
>BG042278
Length = 366
Score = 56.2 bits (134), Expect = 3e-08
Identities = 25/80 (31%), Positives = 40/80 (49%), Gaps = 6/80 (7%)
Frame = +2
Query: 153 FVIGHDCAHKSFSTNKLVEDIVGTLAFLPLIYPYEPWRFKHDKHHAKTNMLKEDTAWQP- 211
+VI H+C H +FS V+D++G L+ PY W+ H +HH+ T L D + P
Sbjct: 5 WVIAHECGHHAFSKYPWVDDVMGLTVHSALLVPYFSWKISHRRHHSNTGFLDSDEVFVPN 184
Query: 212 -----VWQDEFESSPFLRKA 226
W ++ ++P R A
Sbjct: 185 PNSNAAWYTKYLNNPLGRAA 244
>BG650512
Length = 300
Score = 53.5 bits (127), Expect = 2e-07
Identities = 29/87 (33%), Positives = 43/87 (49%), Gaps = 4/87 (4%)
Frame = +3
Query: 302 WMSTFTMVHHTAPH--IPFKNSEEWNAAQAQLNGTVH--CAYPQWIEILCHDINVHIPHH 357
W+ T +HH +P+ EEW+ + L G C WI + HDI H+ HH
Sbjct: 15 WLDLVTYLHHHGHEDKLPWYRGEEWSYLRGGLTGIDRDFC----WINNIHHDIGTHVIHH 182
Query: 358 ISPKIPSYNLRAAHKSIQENWGKYLNE 384
+ P+IP Y+L A ++ + GKY E
Sbjct: 183 LFPQIPHYHLIEATEAAKPVLGKYYRE 263
>BF066409 similar to GP|2613051|gb|A omega-6 desaturase {Arachis hypogaea},
partial (19%)
Length = 232
Score = 47.4 bits (111), Expect = 1e-05
Identities = 22/65 (33%), Positives = 35/65 (53%)
Frame = +2
Query: 139 PLAWAWTGTAVTGFFVIGHDCAHKSFSTNKLVEDIVGTLAFLPLIYPYEPWRFKHDKHHA 198
P+ WA G +T I +C H +FS +L++D+VG + L+ Y +RF +HH+
Sbjct: 14 PIYWADQGCILTVVRAIARECGHHAFSDYQLLDDLVGLVLHSCLLVLYFSYRFSQFRHHS 193
Query: 199 KTNML 203
T L
Sbjct: 194 NTGSL 208
>BE800553 GP|27902573|g microsomal omega-3-fatty acid desaturase {Glycine
max}, partial (34%)
Length = 410
Score = 42.0 bits (97), Expect(2) = 1e-04
Identities = 23/62 (37%), Positives = 34/62 (54%)
Frame = +1
Query: 323 EWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPKIPSYNLRAAHKSIQENWGKYL 382
EW+ + L TV Y WI + HDI H+ HH+ P+IP Y+L A ++ + G+Y
Sbjct: 163 EWSYLRGGLT-TVDRDYG-WINNIHHDIGTHVIHHLFPQIPHYHLVEATQAAKSVLGEYY 336
Query: 383 NE 384
E
Sbjct: 337 RE 342
Score = 21.6 bits (44), Expect(2) = 1e-04
Identities = 7/27 (25%), Positives = 14/27 (50%)
Frame = +2
Query: 289 IKFWLMPWLGYHFWMSTFTMVHHTAPH 315
+K + +P+ + W+ T +HH H
Sbjct: 32 LKLYGIPYWIFVMWLDFVTYLHHHGHH 112
>TC205449 homologue to UP|FD62_SOYBN (P48631) Omega-6 fatty acid desaturase,
endoplasmic reticulum isozyme 2 , partial (40%)
Length = 803
Score = 42.4 bits (98), Expect = 4e-04
Identities = 28/117 (23%), Positives = 51/117 (42%)
Frame = +2
Query: 265 LACVFAFIAIGWPLIIYKTGIMGWIKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKNSEEW 324
L C+ + W + +Y +P L + ++ T + HT P +P S EW
Sbjct: 32 LFCLAMAKGLAWVVCVYG-----------VPLLVVNGFLVLITFLQHTHPALPHYTSSEW 178
Query: 325 NAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPKIPSYNLRAAHKSIQENWGKY 381
+ + L TV Y ++ + + H+ HH+ +P Y+ A K+I+ G+Y
Sbjct: 179 DWLRGAL-ATVDRDYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEY 346
>TC227314 similar to UP|Q9ZRP8 (Q9ZRP8) Delta-8 sphingolipid desaturase,
partial (72%)
Length = 1712
Score = 38.9 bits (89), Expect = 0.005
Identities = 50/242 (20%), Positives = 90/242 (36%), Gaps = 28/242 (11%)
Frame = +1
Query: 155 IGHDCAH----KSFSTNKLVEDIVGT-LAFLPLIYPYEPWRFKHDKHHAKTNMLKEDTAW 209
+GHD H S NKL + + G + + + + W++ H+ HH N L D
Sbjct: 103 VGHDSGHYEVMSSPGYNKLAQILCGNCMTGISIAW----WKWTHNAHHISCNSLDYDPDL 270
Query: 210 Q--PVWQDEFESSPFLRKAIIYGYGPFRCWMSIAHWLVSHFDLKTFRPNEIKRVKISLAC 267
Q PV+ S+ F YG + S++ +L+S+ + RV + L
Sbjct: 271 QHIPVFA---VSTRFFNSIKSCFYGRKLVFDSLSRFLISYQHFTFYPVLCFARVNLYLQT 441
Query: 268 VFAFIAIGWPLIIYKTGIMGWIKFWL--------MPWLGYHFWMSTFTMVHHTAPHIPF- 318
+ + + IMG + FW+ +P G + + H+ F
Sbjct: 442 LLLLFSRR-KVPDRAFNIMGILVFWVWFPLLISSLPNWGERVMFVLASFAVCSIQHLQFC 618
Query: 319 ------------KNSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPKIPSYN 366
N +W + Q GT+ + W++ + + HH+ P++P
Sbjct: 619 LNHFSADVYEGPPNGNDW--FEKQTGGTLDISCSSWMDWFFGGLQFQLEHHLFPRLPRAQ 792
Query: 367 LR 368
LR
Sbjct: 793 LR 798
>TC204935 similar to UP|P93350 (P93350) Plastid omega-3 fatty acid desaturase
, partial (18%)
Length = 574
Score = 36.6 bits (83), Expect = 0.023
Identities = 15/37 (40%), Positives = 22/37 (58%)
Frame = +1
Query: 348 HDINVHIPHHISPKIPSYNLRAAHKSIQENWGKYLNE 384
HDI H+ HH+ P+IP Y L A ++++ G Y E
Sbjct: 10 HDIXTHVIHHLFPQIPHYXLIEAXEAVKPVLGLYXRE 120
>BI426946 similar to GP|12597245|dbj cytochrome b561 {Arabidopsis thaliana},
partial (27%)
Length = 421
Score = 33.1 bits (74), Expect = 0.25
Identities = 18/58 (31%), Positives = 28/58 (48%), Gaps = 1/58 (1%)
Frame = +3
Query: 135 WYLLPLAWAWTGTAVTGFFVIGHDCAHKSFSTNKLVEDIVGTLAFLPLIYP-YEPWRF 191
W LLP +W+W+G++++G +G S ST L P+ P +E W F
Sbjct: 39 WQLLPASWSWSGSSISGVAWLGILITSPSSSTYSL----------FPITIPLFEIWAF 182
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.323 0.138 0.461
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,641,189
Number of Sequences: 63676
Number of extensions: 414822
Number of successful extensions: 2443
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 2374
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2423
length of query: 433
length of database: 12,639,632
effective HSP length: 100
effective length of query: 333
effective length of database: 6,272,032
effective search space: 2088586656
effective search space used: 2088586656
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 60 (27.7 bits)
Medicago: description of AC122160.5