BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121242.7 + phase: 0
(435 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC220812 similar to UP|Q9LRM9 (Q9LRM9) Gb|AAD27719.1, partial (57%) 513 e-146
TC219415 weakly similar to UP|Q9AVE7 (Q9AVE7) Zeaxanthin epoxida... 46 4e-05
AI966377 similar to GP|9857294|dbj CpABA1 {Vigna unguiculata}, p... 40 0.003
AW755720 38 0.010
CO982030 37 0.023
TC228441 similar to UP|Q8GSM8 (Q8GSM8) Squalene monooxygenase 1 ... 35 0.066
TC205901 homologue to UP|Q8GSM8 (Q8GSM8) Squalene monooxygenase ... 35 0.086
TC205902 homologue to UP|Q8GSM9 (Q8GSM9) Squalene monooxygenase ... 34 0.15
TC225479 UP|Q9XE94 (Q9XE94) Geranylgeranyl hydrogenase, partial ... 34 0.15
TC225480 UP|Q9XE94 (Q9XE94) Geranylgeranyl hydrogenase, complete 34 0.15
TC205900 homologue to UP|Q8GSM9 (Q8GSM9) Squalene monooxygenase ... 33 0.25
TC217353 similar to UP|Q9ZSN8 (Q9ZSN8) CTF2A (Fragment), partial... 33 0.25
TC217355 similar to UP|Q9ZSN8 (Q9ZSN8) CTF2A (Fragment), partial... 32 0.56
TC214364 UP|Q6WNU4 (Q6WNU4) Subtilisin-like protease, complete 30 2.1
BI425678 30 2.1
TC217354 similar to UP|Q9SBB5 (Q9SBB5) CTF2A, partial (50%) 30 2.8
TC221587 weakly similar to UP|Q8H9C4 (Q8H9C4) Monooxygenase (Fra... 29 4.7
AW760326 similar to GP|19749359|gb geranylgeranyl hydrogenase {G... 28 6.2
TC203736 similar to UP|O04149 (O04149) Atranbp1a protein, partia... 28 8.1
>TC220812 similar to UP|Q9LRM9 (Q9LRM9) Gb|AAD27719.1, partial (57%)
Length = 1088
Score = 513 bits (1322), Expect = e-146
Identities = 252/298 (84%), Positives = 267/298 (89%)
Frame = +2
Query: 138 SKEPSPAQGHASKLELSDGSSIYAKLVVGADGGKSRVRELAGFKTTGWNYSQNAIICTVE 197
SKEP AQGH ++L+LSDGSSIYAKLVVGADGGKS VRELAG KTTGWNYSQNAIICTVE
Sbjct: 17 SKEPPSAQGHGAELQLSDGSSIYAKLVVGADGGKSHVRELAGIKTTGWNYSQNAIICTVE 196
Query: 198 HTSANHCAWQRFLPNGPIALLPMGENFSNIVWTMSPTESNDRKSITEEMFLKDVNSALDY 257
HT N CAWQRFLP GPIALLPMG+ FSNIVWTMSPTESN+RKSITEE FLKDVN ALDY
Sbjct: 197 HTYENECAWQRFLPTGPIALLPMGDKFSNIVWTMSPTESNNRKSITEEEFLKDVNYALDY 376
Query: 258 GYGPHPTSSSLGARDIFSWLKMDGTLSANEPFEIPPKAIRLASERMVFPLSLRHANSYAT 317
G+GPHPTSS G RD+FSW K D TLS NE FEIPPK RLASERMVFPLSLRHANSYA
Sbjct: 377 GHGPHPTSSLSGTRDVFSWFKTDATLSTNEFFEIPPKVTRLASERMVFPLSLRHANSYAL 556
Query: 318 KRVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDIGEVNLLKKYEQERKP 377
KRVVLIGDAAHT+HPLAGQGVNLGFGDA+SLSR+IAEGIALG+DIGEVN+LKKYE ERK
Sbjct: 557 KRVVLIGDAAHTVHPLAGQGVNLGFGDAFSLSRIIAEGIALGTDIGEVNMLKKYEAERKT 736
Query: 378 ANVTMMAILDGFQKAYSIDFGPFNFLRGAAFSGANFVSPLKRSIISYASGERKLPIFF 435
AN+ MMAILDG QKAYSIDFGPFN LR AAF GAN++SPLKRSIISYASGE KLPIFF
Sbjct: 737 ANIMMMAILDGVQKAYSIDFGPFNILRAAAFHGANYISPLKRSIISYASGEHKLPIFF 910
>TC219415 weakly similar to UP|Q9AVE7 (Q9AVE7) Zeaxanthin epoxidase, partial
(18%)
Length = 448
Score = 45.8 bits (107), Expect = 4e-05
Identities = 26/66 (39%), Positives = 38/66 (57%), Gaps = 1/66 (1%)
Frame = +3
Query: 313 NSYATKRVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDIGEV-NLLKKY 371
N++ RV L+GDAAH + P GQG + D Y L + + GSD EV + L++Y
Sbjct: 231 NTWGIGRVTLLGDAAHPMQPNLGQGGCMAIEDCYQLILELDKVAKHGSDGSEVISALRRY 410
Query: 372 EQERKP 377
E++R P
Sbjct: 411 EKKRIP 428
>AI966377 similar to GP|9857294|dbj CpABA1 {Vigna unguiculata}, partial (26%)
Length = 487
Score = 39.7 bits (91), Expect = 0.003
Identities = 30/95 (31%), Positives = 44/95 (45%), Gaps = 11/95 (11%)
Frame = +2
Query: 320 VVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVI----AEGIALGSDIGEVNLLKKYEQER 375
V L+GD+ H + P GQ + D+Y L+ + + I GS I + LK YE+ER
Sbjct: 185 VTLLGDSIHAMQPNMGQ*GCMAIEDSYQLALELDNAWQQSIKSGSPIDIDSSLKSYERER 364
Query: 376 K------PANVTMMAILDGFQKAY-SIDFGPFNFL 403
+ M A++ KAY + GP FL
Sbjct: 365 RLRVAIVHGMARMAAMMASTYKAYLGVGLGPLEFL 469
>AW755720
Length = 426
Score = 37.7 bits (86), Expect = 0.010
Identities = 18/19 (94%), Positives = 18/19 (94%)
Frame = +1
Query: 2 PMTKQLSVAIIDSNPALSS 20
PMTKQLSVAIIDSNPAL S
Sbjct: 370 PMTKQLSVAIIDSNPALGS 426
>CO982030
Length = 641
Score = 36.6 bits (83), Expect = 0.023
Identities = 24/89 (26%), Positives = 37/89 (40%)
Frame = -3
Query: 306 PLSLRHANSYATKRVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDIGEV 365
P S A+ Y T +L+GDA + HPL G G+ + D L ++ L
Sbjct: 609 PNSSMAADPYPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLKPICDLNDATSLC 430
Query: 366 NLLKKYEQERKPANVTMMAILDGFQKAYS 394
L+ + RKP T+ + K +S
Sbjct: 429 RHLESFYILRKPMASTINTLAGALYKVFS 343
>TC228441 similar to UP|Q8GSM8 (Q8GSM8) Squalene monooxygenase 1 , partial
(39%)
Length = 911
Score = 35.0 bits (79), Expect = 0.066
Identities = 31/127 (24%), Positives = 49/127 (38%), Gaps = 5/127 (3%)
Frame = +3
Query: 312 ANSYATKRVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDIGEVNLLKKY 371
A Y T +L+GD + HPL G G+ + D L ++ D+ + N L KY
Sbjct: 42 AXXYPTPGALLMGDXXNMRHPLTGGGMTVALSDIVLLRNLLRP----LHDLHDANALCKY 209
Query: 372 EQE----RKPANVTMMAILDGFQKAYSIDFGPFN-FLRGAAFSGANFVSPLKRSIISYAS 426
+ RKP T+ + K + P + +R A F + I+ S
Sbjct: 210 LESFYTLRKPVASTINTLAGALYKVFCASPDPASKEMRQACFDYLSLGGVFSDGPIALLS 389
Query: 427 GERKLPI 433
G P+
Sbjct: 390 GLNPRPL 410
>TC205901 homologue to UP|Q8GSM8 (Q8GSM8) Squalene monooxygenase 1 , partial
(35%)
Length = 725
Score = 34.7 bits (78), Expect = 0.086
Identities = 27/114 (23%), Positives = 43/114 (37%), Gaps = 1/114 (0%)
Frame = +3
Query: 321 VLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDIGEVNLLKKYEQERKPANV 380
+L+GDA + HPL G G+ + D L ++ L G L+ + RKP
Sbjct: 3 LLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLRPXRDLNDAPGLCKYLESFYTLRKPVAS 182
Query: 381 TMMAILDGFQKAYSIDFGPF-NFLRGAAFSGANFVSPLKRSIISYASGERKLPI 433
T+ + K + P +R A F + +S SG P+
Sbjct: 183 TINTLAGALYKVFCASPDPARKEMRQACFDYLSLGGLFSEGPVSLLSGLNPRPL 344
>TC205902 homologue to UP|Q8GSM9 (Q8GSM9) Squalene monooxygenase 2 , partial
(65%)
Length = 1161
Score = 33.9 bits (76), Expect = 0.15
Identities = 23/86 (26%), Positives = 39/86 (44%), Gaps = 4/86 (4%)
Frame = +3
Query: 312 ANSYATKRVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDIGEVNLLKKY 371
A+ + T +L+GDA + HPL G G+ + D L +++ SD+ + L KY
Sbjct: 483 ADPHPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLSP----LSDLNDAPTLCKY 650
Query: 372 EQE----RKPANVTMMAILDGFQKAY 393
+ RKP T+ + K +
Sbjct: 651 LESFYTLRKPVASTINTLAGALYKVF 728
>TC225479 UP|Q9XE94 (Q9XE94) Geranylgeranyl hydrogenase, partial (43%)
Length = 937
Score = 33.9 bits (76), Expect = 0.15
Identities = 21/75 (28%), Positives = 33/75 (44%)
Frame = +3
Query: 319 RVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDIGEVNLLKKYEQERKPA 378
RV L+GDAA + +G+G+ + I EG G + E L+KY ++
Sbjct: 183 RVALVGDAAGYVTKCSGEGIYFAAKSGRMCAEAIVEGSENGKRMVEEGDLRKYLEKWDKT 362
Query: 379 NVTMMAILDGFQKAY 393
+LD QK +
Sbjct: 363 YWPTYKVLDILQKVF 407
>TC225480 UP|Q9XE94 (Q9XE94) Geranylgeranyl hydrogenase, complete
Length = 1746
Score = 33.9 bits (76), Expect = 0.15
Identities = 21/75 (28%), Positives = 33/75 (44%)
Frame = +1
Query: 319 RVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDIGEVNLLKKYEQERKPA 378
RV L+GDAA + +G+G+ + I EG G + E L+KY ++
Sbjct: 967 RVALVGDAAGYVTKCSGEGIYFAAKSGRMCAEAIVEGSENGKRMVEEGDLRKYLEKWDKT 1146
Query: 379 NVTMMAILDGFQKAY 393
+LD QK +
Sbjct: 1147 YWPTYKVLDILQKVF 1191
>TC205900 homologue to UP|Q8GSM9 (Q8GSM9) Squalene monooxygenase 2 , partial
(36%)
Length = 825
Score = 33.1 bits (74), Expect = 0.25
Identities = 23/86 (26%), Positives = 38/86 (43%), Gaps = 4/86 (4%)
Frame = +3
Query: 312 ANSYATKRVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDIGEVNLLKKY 371
A+ + T +L+GDA + HPL G G+ + D L ++ SD+ + L KY
Sbjct: 9 ADPHPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLRP----LSDLNDAPTLCKY 176
Query: 372 EQE----RKPANVTMMAILDGFQKAY 393
+ RKP T+ + K +
Sbjct: 177 LESFYTLRKPVASTINTLAGALYKVF 254
>TC217353 similar to UP|Q9ZSN8 (Q9ZSN8) CTF2A (Fragment), partial (42%)
Length = 792
Score = 33.1 bits (74), Expect = 0.25
Identities = 21/64 (32%), Positives = 30/64 (46%)
Frame = +2
Query: 314 SYATKRVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDIGEVNLLKKYEQ 373
S + RVV++GDA H + P GQG D+ L++ +A I D + Y
Sbjct: 218 SASAGRVVVVGDAWHPMTPNLGQGACCALEDSVVLAKKLARAIN-SEDPSVEEAFRSYGA 394
Query: 374 ERKP 377
ER P
Sbjct: 395 ERWP 406
>TC217355 similar to UP|Q9ZSN8 (Q9ZSN8) CTF2A (Fragment), partial (29%)
Length = 631
Score = 32.0 bits (71), Expect = 0.56
Identities = 21/59 (35%), Positives = 30/59 (50%)
Frame = +2
Query: 319 RVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDIGEVNLLKKYEQERKP 377
RVV++GDA H + P GQG D+ L++ +A I + I E + Y ER P
Sbjct: 104 RVVVVGDAWHPMTPNLGQGACCALEDSVVLAKKLARAINV**PIVE-EAFRPYGTERWP 277
>TC214364 UP|Q6WNU4 (Q6WNU4) Subtilisin-like protease, complete
Length = 2667
Score = 30.0 bits (66), Expect = 2.1
Identities = 17/44 (38%), Positives = 26/44 (58%)
Frame = -2
Query: 384 AILDGFQKAYSIDFGPFNFLRGAAFSGANFVSPLKRSIISYASG 427
A++ GF+ + +I FGPF+ AA +GA VS + YA+G
Sbjct: 1610 AVISGFRISGTIVFGPFD--ENAAINGAGLVSN*VFGCVIYATG 1485
>BI425678
Length = 425
Score = 30.0 bits (66), Expect = 2.1
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +1
Query: 312 ANSYATKRVVLIGDAAHTIHPLAGQGVNLGFGD 344
A+ Y T +L+GDA + HPL G G+ + D
Sbjct: 289 ASPYPTPGALLMGDAFNMRHPLTGGGMTVALSD 387
>TC217354 similar to UP|Q9SBB5 (Q9SBB5) CTF2A, partial (50%)
Length = 859
Score = 29.6 bits (65), Expect = 2.8
Identities = 13/30 (43%), Positives = 20/30 (66%)
Frame = +3
Query: 151 LELSDGSSIYAKLVVGADGGKSRVRELAGF 180
LEL DGS + A++V+ DG +S + + GF
Sbjct: 513 LELVDGSKLLARIVIECDGIRSPIAKWMGF 602
>TC221587 weakly similar to UP|Q8H9C4 (Q8H9C4) Monooxygenase (Fragment),
partial (29%)
Length = 608
Score = 28.9 bits (63), Expect = 4.7
Identities = 29/100 (29%), Positives = 43/100 (43%), Gaps = 10/100 (10%)
Frame = +3
Query: 295 AIRLASERMVFPLSLRHANSYATKRVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAE 354
A +LA R P L N + + + GDA H + P GQG D L+R +A
Sbjct: 117 AFQLAPLRYRHPWELMFGN-ISKGNICVGGDAFHPMTPDLGQGGCCALEDGIVLARCLA- 290
Query: 355 GIALGSDIGEVN----------LLKKYEQERKPANVTMMA 384
A I E + LKKY +ER+ ++ ++A
Sbjct: 291 -AAFSKHIKEKDEEDQFKRIEGSLKKYAKERRWRSIDVIA 407
>AW760326 similar to GP|19749359|gb geranylgeranyl hydrogenase {Glycine max},
partial (21%)
Length = 304
Score = 28.5 bits (62), Expect = 6.2
Identities = 17/53 (32%), Positives = 24/53 (45%)
Frame = +2
Query: 319 RVVLIGDAAHTIHPLAGQGVNLGFGDAYSLSRVIAEGIALGSDIGEVNLLKKY 371
R+ L+GDAA + +G+G+ S I EG G + E L KY
Sbjct: 143 RMALVGDAAGYVTKCSGEGIYFATLSGRMCSYAIDEGYENGKMMVEEGYLTKY 301
>TC203736 similar to UP|O04149 (O04149) Atranbp1a protein, partial (48%)
Length = 509
Score = 28.1 bits (61), Expect = 8.1
Identities = 13/27 (48%), Positives = 19/27 (70%)
Frame = -2
Query: 144 AQGHASKLELSDGSSIYAKLVVGADGG 170
A G ++ LEL DGS + + ++V ADGG
Sbjct: 238 AGGDSNLLELDDGSDVCSGVLVVADGG 158
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.133 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,674,758
Number of Sequences: 63676
Number of extensions: 256271
Number of successful extensions: 1375
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 1369
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1375
length of query: 435
length of database: 12,639,632
effective HSP length: 100
effective length of query: 335
effective length of database: 6,272,032
effective search space: 2101130720
effective search space used: 2101130720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC121242.7