BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121239.10 - phase: 0
(90 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC230171 similar to UP|Q9FFC7 (Q9FFC7) Alanyl-tRNA synthetase, p... 169 2e-43
BI701986 32 0.054
BM732165 homologue to SP|Q9TV66|HCN4 Potassium/sodium hyperpolar... 32 0.070
CF921682 30 0.21
TC230795 weakly similar to UP|O23299 (O23299) Carnitine racemase... 30 0.21
TC234951 weakly similar to UP|DHX9_MOUSE (O70133) ATP-dependent ... 30 0.27
AI416502 30 0.27
TC232500 similar to GB|AAL84954.1|19310435|AY078954 F17H15.1/F17... 29 0.35
TC209672 similar to GB|AAM19966.1|20466089|AY098956 At2g32600/T2... 29 0.46
TC205978 homologue to UP|Q6XPS8 (Q6XPS8) Drought-induced protein... 29 0.46
TC205979 homologue to UP|Q6XPS8 (Q6XPS8) Drought-induced protein... 29 0.46
TC232813 weakly similar to UP|FLS_EUSGR (Q9M547) Flavonol syntha... 28 0.60
TC219511 similar to UP|O82687 (O82687) Tyrosine phosphatase 1, p... 28 0.60
TC216757 similar to PIR|T05768|T05768 subtilisin-like proteinase... 27 1.3
TC212222 similar to UP|Q9LR47 (Q9LR47) T25N20.12, partial (30%) 27 1.3
BM891498 27 1.3
TC211249 similar to UP|FXE3_MOUSE (Q9QY14) Forkhead box protein ... 27 1.3
TC210831 27 1.3
AW278013 similar to SP|P27484|GRP2 Glycine-rich protein 2. [Wood... 27 1.7
CF921716 27 1.7
>TC230171 similar to UP|Q9FFC7 (Q9FFC7) Alanyl-tRNA synthetase, partial (16%)
Length = 813
Score = 169 bits (428), Expect = 2e-43
Identities = 81/90 (90%), Positives = 86/90 (95%)
Frame = +2
Query: 1 METLADPAAIVLGSCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFA 60
+ETL DPAA++LGSCPGEGKVSLVAAFTPGVV QGIQAGKFIGQIAKLCGGGGGGRPNFA
Sbjct: 200 LETLTDPAAVILGSCPGEGKVSLVAAFTPGVVDQGIQAGKFIGQIAKLCGGGGGGRPNFA 379
Query: 61 QAGGRKPENLAGALEKAKSELIAYLCEKGN 90
QAGGRKPENLA AL+KA+SELIA LCEKGN
Sbjct: 380 QAGGRKPENLASALDKARSELIATLCEKGN 469
>BI701986
Length = 422
Score = 32.0 bits (71), Expect = 0.054
Identities = 20/52 (38%), Positives = 27/52 (51%)
Frame = -1
Query: 19 GKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRPNFAQAGGRKPENL 70
G S V+ F PG+ G G++ G + L GGGGGG + G + ENL
Sbjct: 179 GSSSFVSEFPPGLGGDGVEGESRTGFL--LGGGGGGGGGDE*S*GVAEAENL 30
>BM732165 homologue to SP|Q9TV66|HCN4 Potassium/sodium
hyperpolarization-activated cyclic nucleotide-gated
channel 4, partial (2%)
Length = 423
Score = 31.6 bits (70), Expect = 0.070
Identities = 15/37 (40%), Positives = 20/37 (53%)
Frame = -3
Query: 19 GKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGG 55
G S V+ F PG+ G G++ G + GGGGGG
Sbjct: 148 GSSSFVSEFPPGLGGDGVEGESRTGFLLGGGGGGGGG 38
>CF921682
Length = 513
Score = 30.0 bits (66), Expect = 0.21
Identities = 18/51 (35%), Positives = 19/51 (36%)
Frame = -1
Query: 7 PAAIVLGSCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRP 57
P G CP F PGV G + G F GGGGGG P
Sbjct: 360 PLGFPPGKCPFFPPGKNPGGFFPGVPGWASRGGGFGFFFPPFLGGGGGGDP 208
Score = 24.6 bits (52), Expect = 8.6
Identities = 17/46 (36%), Positives = 20/46 (42%), Gaps = 1/46 (2%)
Frame = +3
Query: 28 TPGVVGQGIQAGKFIGQIAKLCG-GGGGGRPNFAQAGGRKPENLAG 72
TP G + G F G K GGGGG P GG+K + G
Sbjct: 300 TPPGFSPGEKRGIFRGGTPKGGNPGGGGGPPRKKGVGGKKKKKGGG 437
>TC230795 weakly similar to UP|O23299 (O23299) Carnitine racemase like
protein (AT4g14430/dl3255c), partial (47%)
Length = 749
Score = 30.0 bits (66), Expect = 0.21
Identities = 16/35 (45%), Positives = 23/35 (65%)
Frame = -1
Query: 33 GQGIQAGKFIGQIAKLCGGGGGGRPNFAQAGGRKP 67
G+G + G+ G+ + CGGGGGGR +AG R+P
Sbjct: 272 GRGARRGRGGGR--RTCGGGGGGR----RAGRRRP 186
>TC234951 weakly similar to UP|DHX9_MOUSE (O70133) ATP-dependent RNA helicase
A (Nuclear DNA helicase II) (NDH II) (DEAH-box protein
9) (mHEL-5), partial (3%)
Length = 460
Score = 29.6 bits (65), Expect = 0.27
Identities = 18/49 (36%), Positives = 22/49 (44%), Gaps = 8/49 (16%)
Frame = -3
Query: 24 VAAFTPGVVGQGIQAGKFIGQIAKL--------CGGGGGGRPNFAQAGG 64
+A PGV G FIG A L GGGGGG+ ++ GG
Sbjct: 188 IACTGPGVTCADPGVGVFIGGAATLGGFGGVVSSGGGGGGKDGLSELGG 42
>AI416502
Length = 238
Score = 29.6 bits (65), Expect = 0.27
Identities = 13/17 (76%), Positives = 14/17 (81%), Gaps = 1/17 (5%)
Frame = -1
Query: 50 GGGGGGRPNFAQA-GGR 65
GGGGGG+PNFA GGR
Sbjct: 70 GGGGGGKPNFASPFGGR 20
>TC232500 similar to GB|AAL84954.1|19310435|AY078954 F17H15.1/F17H15.1
{Arabidopsis thaliana;} , partial (3%)
Length = 425
Score = 29.3 bits (64), Expect = 0.35
Identities = 24/76 (31%), Positives = 36/76 (46%), Gaps = 5/76 (6%)
Frame = +2
Query: 19 GKVSLVAAFTPGVVGQGIQ---AGKFIGQI-AKLCGGGGGGRPNFAQAGGRKPENLAGAL 74
G V +V GV G G +G F+G + +L GGGGGR GG GA+
Sbjct: 77 GAVVVVLGAFGGVEGSGAAEEASGDFLGALFGELEVGGGGGRGRDVVVGGGWRRRRVGAV 256
Query: 75 -EKAKSELIAYLCEKG 89
E +++ ++ L +G
Sbjct: 257 GESSRAVVVVVLALEG 304
>TC209672 similar to GB|AAM19966.1|20466089|AY098956 At2g32600/T26B15.16
{Arabidopsis thaliana;} , partial (25%)
Length = 682
Score = 28.9 bits (63), Expect = 0.46
Identities = 22/55 (40%), Positives = 24/55 (43%), Gaps = 6/55 (10%)
Frame = -1
Query: 17 GEGKVSLVAAF-----TPGVVGQGIQAGKFIGQI-AKLCGGGGGGRPNFAQAGGR 65
G G LVAA PG+ G G G IG CGGGGGG GG+
Sbjct: 379 GAGLGPLVAAIGGGGIDPGIGGGGALGGFAIGAPGGSPCGGGGGGGSGPWGGGGK 215
>TC205978 homologue to UP|Q6XPS8 (Q6XPS8) Drought-induced protein 1, complete
Length = 645
Score = 28.9 bits (63), Expect = 0.46
Identities = 21/56 (37%), Positives = 31/56 (54%), Gaps = 2/56 (3%)
Frame = +3
Query: 14 SCPGEGKVS-LVAAFTPGVVGQGIQAGKFIGQIA-KLCGGGGGGRPNFAQAGGRKP 67
+C G G+V+ L + ++ G VG +G G++A CGG G GRP A+ R P
Sbjct: 252 TCNGTGRVTCLCSRWSDGDVGCSTCSGS--GRMACSSCGGSGTGRPLPAKIAIRPP 413
>TC205979 homologue to UP|Q6XPS8 (Q6XPS8) Drought-induced protein 1, complete
Length = 638
Score = 28.9 bits (63), Expect = 0.46
Identities = 21/56 (37%), Positives = 31/56 (54%), Gaps = 2/56 (3%)
Frame = +1
Query: 14 SCPGEGKVS-LVAAFTPGVVGQGIQAGKFIGQIA-KLCGGGGGGRPNFAQAGGRKP 67
+C G G+V+ L + ++ G VG +G G++A CGG G GRP A+ R P
Sbjct: 193 TCNGTGRVTCLCSRWSDGDVGCSTCSGS--GRMACSSCGGSGTGRPLPAKIAIRPP 354
>TC232813 weakly similar to UP|FLS_EUSGR (Q9M547) Flavonol synthase (FLS) ,
partial (12%)
Length = 549
Score = 28.5 bits (62), Expect = 0.60
Identities = 17/45 (37%), Positives = 23/45 (50%), Gaps = 1/45 (2%)
Frame = -1
Query: 14 SCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGG-GGGRP 57
SC G +L P +VG G+ G+ G + + GGG GGG P
Sbjct: 153 SCHGLSMKNL*WVLDPSLVGSGLYPGQ--GLVVVVVGGGLGGGEP 25
>TC219511 similar to UP|O82687 (O82687) Tyrosine phosphatase 1, partial (56%)
Length = 659
Score = 28.5 bits (62), Expect = 0.60
Identities = 19/51 (37%), Positives = 24/51 (46%), Gaps = 1/51 (1%)
Frame = -3
Query: 35 GIQAGKFIGQI-AKLCGGGGGGRPNFAQAGGRKPENLAGALEKAKSELIAY 84
G+ G+ QI + CGGGGGG A GG A E + +LI Y
Sbjct: 156 GVIGGEIEFQILGRQCGGGGGGGGGVAGHGGSPAGTRASRDEVPQLQLI*Y 4
>TC216757 similar to PIR|T05768|T05768 subtilisin-like proteinase -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(20%)
Length = 1126
Score = 27.3 bits (59), Expect = 1.3
Identities = 10/15 (66%), Positives = 12/15 (79%)
Frame = +1
Query: 43 GQIAKLCGGGGGGRP 57
G+ A+LCG GGGG P
Sbjct: 490 GEEAELCGDGGGGHP 534
>TC212222 similar to UP|Q9LR47 (Q9LR47) T25N20.12, partial (30%)
Length = 645
Score = 27.3 bits (59), Expect = 1.3
Identities = 14/37 (37%), Positives = 18/37 (47%)
Frame = +2
Query: 49 CGGGGGGRPNFAQAGGRKPENLAGALEKAKSELIAYL 85
CGGGGG P F + G P + L +K + YL
Sbjct: 461 CGGGGGAGPAFNEDGYAMPGHSRYCLVASKQMVGIYL 571
>BM891498
Length = 381
Score = 27.3 bits (59), Expect = 1.3
Identities = 12/29 (41%), Positives = 16/29 (54%)
Frame = -2
Query: 36 IQAGKFIGQIAKLCGGGGGGRPNFAQAGG 64
++ G A +CGGGGG R A+ GG
Sbjct: 185 VERGGTAAASAAVCGGGGGDRRAEAEEGG 99
Score = 24.6 bits (52), Expect = 8.6
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = +3
Query: 49 CGGGGGGRPN 58
CGGGGGG P+
Sbjct: 150 CGGGGGGAPS 179
>TC211249 similar to UP|FXE3_MOUSE (Q9QY14) Forkhead box protein E3, partial
(5%)
Length = 536
Score = 27.3 bits (59), Expect = 1.3
Identities = 15/33 (45%), Positives = 17/33 (51%)
Frame = -1
Query: 24 VAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGR 56
V TPG+ + G IG K GGGGGGR
Sbjct: 449 VGTGTPGIPWANVLGG--IGSTGKGGGGGGGGR 357
>TC210831
Length = 897
Score = 27.3 bits (59), Expect = 1.3
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +2
Query: 33 GQGIQAGKFIGQIAKLCGGGGGG 55
G G G +G A++CGGGG G
Sbjct: 323 GGGATDGVVVGWRARICGGGGDG 391
>AW278013 similar to SP|P27484|GRP2 Glycine-rich protein 2. [Wood tobacco]
{Nicotiana sylvestris}, partial (48%)
Length = 344
Score = 26.9 bits (58), Expect = 1.7
Identities = 10/16 (62%), Positives = 12/16 (74%)
Frame = +1
Query: 50 GGGGGGRPNFAQAGGR 65
GGGGGGR ++ GGR
Sbjct: 265 GGGGGGRGSYGGGGGR 312
>CF921716
Length = 367
Score = 26.9 bits (58), Expect = 1.7
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = +2
Query: 50 GGGGGGRPNFAQAGGRKPEN 69
GGGGG P + GG PEN
Sbjct: 242 GGGGGKTPQNPRGGGPPPEN 301
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.315 0.139 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,357,677
Number of Sequences: 63676
Number of extensions: 39571
Number of successful extensions: 623
Number of sequences better than 10.0: 88
Number of HSP's better than 10.0 without gapping: 532
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 608
length of query: 90
length of database: 12,639,632
effective HSP length: 66
effective length of query: 24
effective length of database: 8,437,016
effective search space: 202488384
effective search space used: 202488384
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 52 (24.6 bits)
Medicago: description of AC121239.10