BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121237.9 + phase: 0 /pseudo
(652 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC222972 similar to UP|Q6T303 (Q6T303) Ferric reductase, partial... 216 3e-56
TC227997 similar to UP|Q9LTA0 (Q9LTA0) FRO2-like protein; NADPH ... 197 2e-50
BG511181 weakly similar to GP|15341529|gb| ferric-chelate reduct... 117 1e-26
CO983033 91 1e-18
TC215541 similar to UP|Q84KK7 (Q84KK7) Respiratory burst oxidase... 89 8e-18
TC213222 83 4e-16
TC227998 weakly similar to UP|Q9LT99 (Q9LT99) FRO1-like protein;... 68 1e-11
TC211062 similar to UP|Q948T9 (Q948T9) Respiratory burst oxidase... 63 4e-10
BI787197 similar to GP|16549089|db respiratory burst oxidase hom... 60 2e-09
BF424072 47 3e-05
TC215540 similar to UP|Q84KK7 (Q84KK7) Respiratory burst oxidase... 32 1e-04
TC224449 similar to UP|Q6L8G3 (Q6L8G3) Ferric reductase, partial... 43 5e-04
TC215539 similar to UP|Q84KK7 (Q84KK7) Respiratory burst oxidase... 39 0.006
TC231928 similar to UP|Q9ZPN0 (Q9ZPN0) Cytochrome b5 reductase, ... 33 0.52
TC222081 UP|Q43461 (Q43461) Nitrate reductase (Fragment) , complete 32 0.68
BE607523 32 0.89
TC218022 homologue to UP|Q9SL05 (Q9SL05) Expressed protein (At2g... 32 0.89
BQ741940 homologue to GP|18182311|gb| GT-2 factor {Glycine max},... 31 1.5
BI968357 31 1.5
TC225801 similar to UP|Q8H179 (Q8H179) Eukaryotic initiation fac... 31 1.5
>TC222972 similar to UP|Q6T303 (Q6T303) Ferric reductase, partial (27%)
Length = 764
Score = 216 bits (550), Expect = 3e-56
Identities = 104/202 (51%), Positives = 140/202 (68%)
Frame = +2
Query: 451 KQEPSKDTQKQIQTIWFKTNLSDCPISAVLGPNNWLWLGAIITSSFVMFLLFLGIVTRYY 510
K+E + Q +Q IWFK +D PISA+LGPNNWLWL AII+SSF++FL+ +GI+TR+
Sbjct: 5 KEEHRLENQIHLQQIWFKPKATDAPISAILGPNNWLWLCAIISSSFIIFLILIGIITRFI 184
Query: 511 IYPIENNSGEVYNWTYGVMWYMFSFCSCICICSSVVFLWLKRLNKLENKHIMNVEVSTPA 570
I+PI++NS ++++ +F+ C I + +S LW K+ N E K I N+E S+ A
Sbjct: 185 IFPIDHNSNKIFSQPLRSFLNIFAICVSISMAASAAVLWNKKYNDREAKQIQNLEGSSSA 364
Query: 571 RSPGSWIYGSERELESLPHQSLVQATNVHFGSRPDLKKILFECEGKDVGVMTCGPRKMRH 630
SP ++ELESLP QSLVQAT VH+G+RPDL+++L E EG VGV GP+KMR
Sbjct: 365 ESPKLNTDEGDKELESLPQQSLVQATKVHYGARPDLRRLLLELEGSRVGVFVSGPKKMRQ 544
Query: 631 EVARICASGLADNLHFESISFN 652
EVA IC+S L +NLHFES SFN
Sbjct: 545 EVAAICSSDLGENLHFESFSFN 610
>TC227997 similar to UP|Q9LTA0 (Q9LTA0) FRO2-like protein; NADPH
oxidase-like, partial (31%)
Length = 1351
Score = 197 bits (500), Expect = 2e-50
Identities = 134/422 (31%), Positives = 218/422 (50%), Gaps = 22/422 (5%)
Frame = +3
Query: 253 LFLIDRHLRFLQSRQHARLLSARLLPCDALELNFSKNPSLYYNPTSLVFINVPKVSKLQW 312
LF++DR LRF QSR+ ++S+R LPC +EL SK SL YN S +F+ V ++S LQW
Sbjct: 3 LFVLDRFLRFCQSRRTVNVISSRCLPCGTVELVLSKPQSLRYNALSFIFVQVRELSWLQW 182
Query: 313 HPFTVTSSCNMETNYLSVAIKNVGSWSNKLYQELSSSSLDH----LNISVEGPYGPHTAQ 368
HPF+V+SS N+L++ IK +G W+ KL Q ++ + SVEGPYG
Sbjct: 183 HPFSVSSSPLDGKNHLAILIKVLGKWTEKLRQRITDVDAQKDSSVITTSVEGPYGHEVPY 362
Query: 369 FLRHEQIVMVSGGSGVTPFISIIRDLIFQSQQ-QEFQPPKLLLVCIFKNYADLTMLDLML 427
L +E +++V+GG G++PF++I+ D++ + ++ + QP +LLV K +L +L +
Sbjct: 363 HLMYENLILVAGGIGLSPFLAILSDILHRVREGKPCQPRNILLVWAVKKSNELPLLSTID 542
Query: 428 PISGLKTRISQLQLQIEAYITREKQEPSKD--TQKQIQTIWFKTNLSDCPISAVLGPNNW 485
S + +++ + I Y+TRE P ++ + K I++ F SDC +S ++G +
Sbjct: 543 MESICPSFSNKVNIDIHIYVTRESDPPLEEGYSYKPIKSS-FCPMASDCGMSVLVGTGDN 719
Query: 486 LWLGAIITSSFVMFLLFLGIVTRYYIYPIENNSGEVYNWTY-GVMWYMFSFCSCICICSS 544
W G + SS V F++ L ++ YY+ P + W Y G+++ + S + S
Sbjct: 720 FWSGLYVISSTVGFVILLALLYVYYVTPF-----HIETWWYRGLLYVICMVASVVIFGGS 884
Query: 545 VVFLWLKRLNKLENKHIMNVEVSTPARSPGS---WIYGSERELESLPHQ-SLVQATNVHF 600
VV +W HI + S S + I+ + P Q S+ ++T + +
Sbjct: 885 VVAMW----------HIWEKQNSLKDNSNDTKVDKIHQNGSLTPKAPSQVSIAKSTVIRY 1034
Query: 601 GSRPDLKKILFECEGK----DVGVMTCGPRKMRHEVARICASGLADN------LHFESIS 650
GSRPD K+I K DVG++ CGP ++ VA S HF S S
Sbjct: 1035GSRPDFKEIYESMSEKWGLVDVGIIVCGPSTLQTSVAEEIRSHSMTRQRHHPIFHFHSHS 1214
Query: 651 FN 652
F+
Sbjct: 1215FD 1220
>BG511181 weakly similar to GP|15341529|gb| ferric-chelate reductase {Pisum
sativum}, partial (10%)
Length = 314
Score = 117 bits (294), Expect = 1e-26
Identities = 62/104 (59%), Positives = 78/104 (74%), Gaps = 2/104 (1%)
Frame = +1
Query: 380 GGSGVTPFISIIRDLIFQSQQQEFQPPKLLLVCIFKNYADLTMLDLMLPISGLKTR--IS 437
GGSG+TPFISIIR LIF++ + + P++LLVC FK DLT +DL+LP+S T IS
Sbjct: 1 GGSGITPFISIIRSLIFKANTEGSKTPRVLLVCAFKKSIDLTTIDLILPVSATCTAFDIS 180
Query: 438 QLQLQIEAYITREKQEPSKDTQKQIQTIWFKTNLSDCPISAVLG 481
+LQLQIEAY+TREKQ P + +K IQT+WFK N D P+SAVLG
Sbjct: 181 RLQLQIEAYVTREKQ-PDMNDKKLIQTLWFKPNALDEPVSAVLG 309
>CO983033
Length = 614
Score = 91.3 bits (225), Expect = 1e-18
Identities = 45/116 (38%), Positives = 71/116 (60%), Gaps = 7/116 (6%)
Frame = -2
Query: 264 QSRQHARLLSARLLPCDALELNFSKNPSLYYNPTSLVFINVPKVSKLQWHPFTVTSSCNM 323
QS ++SAR+ P A+EL ++P + YNPT ++++ +P +S LQWH F++ SS
Sbjct: 613 QSSPKTCMVSARIFPSRAVELILPEDPGMKYNPTCVIYLKIPTISHLQWHSFSIISSSRA 434
Query: 324 ETNYLSVAIKNVGSWSNKLYQELSSSSLDH-------LNISVEGPYGPHTAQFLRH 372
+ + LSV IK G W+N LY +L + LD + +++EGPYGP + FLR+
Sbjct: 433 DDHILSVIIKCEGWWANSLY-DLIHAELDKTADTRKGIPVAIEGPYGPASLDFLRY 269
>TC215541 similar to UP|Q84KK7 (Q84KK7) Respiratory burst oxidase homolog,
partial (54%)
Length = 1695
Score = 88.6 bits (218), Expect = 8e-18
Identities = 57/217 (26%), Positives = 100/217 (45%), Gaps = 35/217 (16%)
Frame = +3
Query: 221 FFYTHHLYILYILFFAIHA----------GVGSMCVIAPGVFLFLIDRHLR-FLQSRQHA 269
F +HHL+++ +H + +A + ++ ++R +R F S +
Sbjct: 468 FGISHHLFVIVYALLVVHGIKLYLTKEWYKKTTWMYLAIPITIYALERLVRAFRSSIKSV 647
Query: 270 RLLSARLLPCDALELNFSKNPSLYYNPTSLVFINVPKVSKLQWHPFTVTSSCNMETNYLS 329
R+L L P + L L SK Y +F+N VS +WHPF++TS+ + +YLS
Sbjct: 648 RILKVTLYPGNVLSLKMSKPHGFSYKSGQYMFVNCAAVSPFEWHPFSITSA--PDDDYLS 821
Query: 330 VAIKNVGSWSNKLYQELSSSSLDHLN----------------------ISVEGPYGPHTA 367
V IK +G W+ L + + + LN + V+GPYG
Sbjct: 822 VHIKILGDWTRSLKAKFTQACQQPLNGQSGLLRAECLKGDNSPSSFPKVLVDGPYGAPAQ 1001
Query: 368 QFLRHEQIVMVSGGSGVTPFISIIRDLI--FQSQQQE 402
+ +E +++V G G TP ISI++D++ F++ +E
Sbjct: 1002DYREYEVVLLVGLGIGATPMISILKDMVNNFKANDEE 1112
>TC213222
Length = 420
Score = 82.8 bits (203), Expect = 4e-16
Identities = 36/65 (55%), Positives = 46/65 (70%)
Frame = +1
Query: 588 PHQSLVQATNVHFGSRPDLKKILFECEGKDVGVMTCGPRKMRHEVARICASGLADNLHFE 647
P + LVQ VH+G RP++KKIL C G +GV+ GPRKMRHEV +C S D+LHFE
Sbjct: 1 PTRPLVQTAKVHYGERPNIKKILSGCNGSSIGVLVSGPRKMRHEVGSLCTSCSTDDLHFE 180
Query: 648 SISFN 652
S+SF+
Sbjct: 181 SLSFS 195
>TC227998 weakly similar to UP|Q9LT99 (Q9LT99) FRO1-like protein; NADPH
oxidase-like, partial (9%)
Length = 792
Score = 67.8 bits (164), Expect = 1e-11
Identities = 54/199 (27%), Positives = 87/199 (43%), Gaps = 13/199 (6%)
Frame = +1
Query: 467 FKTNLSDCPISAVLGPNNWLWLGAIITSSFVMFLLFLGIVTRYYIYPIENNSGEVYNWTY 526
F SDC +S + G + W + SS V F++ L ++ YY+ P + W Y
Sbjct: 13 FXXMASDCGMSVLXGTXDNFWXXLYVISSTVGFVILLALLYVYYVTPF-----HIETWWY 177
Query: 527 -GVMWYMFSFCSCICICSSVVFLW--LKRLNKLENKHIMNVEVSTPARSPGSWIYGSERE 583
G+++ + S + SVV +W ++ N L++K S + GS
Sbjct: 178 RGLLYVICMVASVVIFGGSVVAMWHIWEKQNSLKDK-------SNDTKVDKIHQNGSLAT 336
Query: 584 LESLPHQSLVQATNVHFGSRPDLKKILFECEGK----DVGVMTCGPRKMRHEVARICASG 639
+ S+ ++T +H+GSRP+ K+I K DVGV+ CGP ++ VA S
Sbjct: 337 KDQSQDSSIAKSTVIHYGSRPNFKEIYDSISEKWGLVDVGVIVCGPSTLQTSVAEEIRSH 516
Query: 640 LADN------LHFESISFN 652
HF S SF+
Sbjct: 517 SMTRQRHHSIFHFHSHSFD 573
>TC211062 similar to UP|Q948T9 (Q948T9) Respiratory burst oxidase homolog,
partial (23%)
Length = 612
Score = 63.2 bits (152), Expect = 4e-10
Identities = 40/146 (27%), Positives = 73/146 (49%), Gaps = 13/146 (8%)
Frame = +3
Query: 210 IPQIRRKMY--EVFFYTHHLYILYILFFAIHAGV----------GSMCVIAPGVFLFLID 257
+P+ +K+ F+Y+HHL+ + + F IH + +A + L+ +
Sbjct: 63 LPKTLKKLTGCNAFWYSHHLFDIDYVLFIIHGYFLYLSKKWYKKTTWMYLAVPMILYGCE 242
Query: 258 RHLR-FLQSRQHARLLSARLLPCDALELNFSKNPSLYYNPTSLVFINVPKVSKLQWHPFT 316
R LR F + R+L + P + L L+ SK Y+ +++N VS +WHP +
Sbjct: 243 RLLRAFRSGYKSVRILKVAVYPGNVLALHVSKPQGFKYSSGQYIYVNCSDVSPFEWHPCS 422
Query: 317 VTSSCNMETNYLSVAIKNVGSWSNKL 342
+TS+ +YLSV I+ +G W+++L
Sbjct: 423 ITSAPG--DDYLSVHIRTLGDWTSQL 494
>BI787197 similar to GP|16549089|db respiratory burst oxidase homolog
{Solanum tuberosum}, partial (16%)
Length = 422
Score = 60.5 bits (145), Expect = 2e-09
Identities = 31/97 (31%), Positives = 55/97 (55%), Gaps = 1/97 (1%)
Frame = +2
Query: 247 IAPGVFLFLIDRHLR-FLQSRQHARLLSARLLPCDALELNFSKNPSLYYNPTSLVFINVP 305
+A + L+ +R LR F + ++L + P + L L+ SK Y+ +F+N P
Sbjct: 47 LAIPMILYACERLLRAFRSGYKSVKILKVAVYPGNVLALHMSKPQGFKYSSGQYIFVNCP 226
Query: 306 KVSKLQWHPFTVTSSCNMETNYLSVAIKNVGSWSNKL 342
VS QWHPF++TS+ +Y+SV I+ +G W++++
Sbjct: 227 DVSPFQWHPFSITSAPG--DDYVSVHIRTLGDWTSQV 331
>BF424072
Length = 384
Score = 47.0 bits (110), Expect = 3e-05
Identities = 22/66 (33%), Positives = 38/66 (57%)
Frame = +2
Query: 9 LRIIFLVMFLGWLSVWILLPTKIYKKIWAPMLQNKFNSTYFRGQGNYVFVFTFPMMFIGA 68
L+++ + +F GW+S+W+L PT+I+ W ++ N T F G V+TFP++ I
Sbjct: 74 LKLLIIFLFAGWVSLWLLKPTQIWTTKW-KHAEDSANDTIFGYYGLSFAVYTFPIVAIAI 250
Query: 69 LSCIYL 74
L + L
Sbjct: 251 LGLLLL 268
>TC215540 similar to UP|Q84KK7 (Q84KK7) Respiratory burst oxidase homolog,
partial (14%)
Length = 406
Score = 32.3 bits (72), Expect(2) = 1e-04
Identities = 19/86 (22%), Positives = 40/86 (46%), Gaps = 11/86 (12%)
Frame = +1
Query: 218 YEVFFYTHHLYILYILFFAIHA----------GVGSMCVIAPGVFLFLIDRHLRFLQSR- 266
+ F+Y+HHL+++ +H + +A + +++ +R R L+S
Sbjct: 43 FNAFWYSHHLFVIVYTLLIVHGIKLYLTKEWYKKTTWMYLAIPIIIYVSERLTRALRSSI 222
Query: 267 QHARLLSARLLPCDALELNFSKNPSL 292
+ R+L + P + L L+ SK P +
Sbjct: 223 KPVRILKVAVYPGNVLSLHMSKPPRI 300
Score = 31.6 bits (70), Expect(2) = 1e-04
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +2
Query: 294 YNPTSLVFINVPKVSKLQWHPFTVTSS 320
Y +F+N VS +WHPF++TS+
Sbjct: 305 YKSGQYMFLNCAAVSPFEWHPFSITSA 385
>TC224449 similar to UP|Q6L8G3 (Q6L8G3) Ferric reductase, partial (12%)
Length = 453
Score = 42.7 bits (99), Expect = 5e-04
Identities = 16/45 (35%), Positives = 29/45 (63%)
Frame = +3
Query: 131 TRMSSILPLVGLTSESSIKYHIWLGQLSMVLFAAHSIGFIIYWAI 175
+R S +L + + E + +YH+WLG L+MV+ H + ++I WA+
Sbjct: 318 SRGSVLLRYIDIPFEHATRYHVWLGHLTMVILTVHRLLYVIAWAM 452
>TC215539 similar to UP|Q84KK7 (Q84KK7) Respiratory burst oxidase homolog,
partial (23%)
Length = 1121
Score = 39.3 bits (90), Expect = 0.006
Identities = 15/40 (37%), Positives = 26/40 (64%)
Frame = +2
Query: 356 ISVEGPYGPHTAQFLRHEQIVMVSGGSGVTPFISIIRDLI 395
+ V+GPYG + +E +++V G G TP ISI++D++
Sbjct: 104 VLVDGPYGAPAQDYREYEVVLLVGLGIGATPMISILKDMV 223
>TC231928 similar to UP|Q9ZPN0 (Q9ZPN0) Cytochrome b5 reductase, partial
(83%)
Length = 854
Score = 32.7 bits (73), Expect = 0.52
Identities = 21/72 (29%), Positives = 40/72 (55%)
Frame = +1
Query: 356 ISVEGPYGPHTAQFLRHEQIVMVSGGSGVTPFISIIRDLIFQSQQQEFQPPKLLLVCIFK 415
++V GP G + + + M++GGSG+TP +IR ++ + + K+ LV
Sbjct: 454 LAVRGPKGRFSYKPGQARAFGMIAGGSGITPMFQLIRAILENPKDK----TKVNLV---- 609
Query: 416 NYADLTMLDLML 427
YA++T+ D++L
Sbjct: 610 -YANVTVDDILL 642
>TC222081 UP|Q43461 (Q43461) Nitrate reductase (Fragment) , complete
Length = 959
Score = 32.3 bits (72), Expect = 0.68
Identities = 35/144 (24%), Positives = 64/144 (44%), Gaps = 16/144 (11%)
Frame = +1
Query: 300 VFINVPKVSKLQWHPFTVTSSCNMETNYLSVAIKNVGSWSNKLYQE--LSSSSLDHLNIS 357
+F+ KL +T TSS + E + +AIK + + L S LD L+I
Sbjct: 190 IFLCATINEKLCMRAYTPTSSVD-EVGFFDLAIKVYFKGVHPKFPRGGLMSQHLDSLSIG 366
Query: 358 ----VEGPYG----PHTAQFLRH------EQIVMVSGGSGVTPFISIIRDLIFQSQQQEF 403
V+GP G FL H +++ M++GG+G+TP + + ++ +
Sbjct: 367 SVLDVKGPLGHIEYTGRGNFLVHGKQRFAKRLAMLAGGTGITPIYQVAQAILKDPE---- 534
Query: 404 QPPKLLLVCIFKNYADLTMLDLML 427
P ++ +V K D+ + + M+
Sbjct: 535 DPTEMHVVYANKTEDDILLKEEMM 606
>BE607523
Length = 369
Score = 32.0 bits (71), Expect = 0.89
Identities = 22/74 (29%), Positives = 39/74 (51%), Gaps = 8/74 (10%)
Frame = +1
Query: 9 LRIIFLVMFLGWLSVWILLPTKIYKKIWAPMLQNK--FNS------TYFRGQGNYVFVFT 60
++++F+++ L + + LLP KI ++ P L NK FN FR +F+
Sbjct: 121 VQVLFVLLGLKFS*LPQLLPKKIVVEL*LPFLTNKIIFNQHKKIIICSFREIKQIGILFS 300
Query: 61 FPMMFIGALSCIYL 74
F ++F G +SC+ L
Sbjct: 301 FEILFFGVISCLIL 342
>TC218022 homologue to UP|Q9SL05 (Q9SL05) Expressed protein
(At2g05620/T20G20.3), partial (57%)
Length = 750
Score = 32.0 bits (71), Expect = 0.89
Identities = 11/19 (57%), Positives = 18/19 (93%)
Frame = -1
Query: 369 FLRHEQIVMVSGGSGVTPF 387
FLR++ +++V+GGSG+TPF
Sbjct: 57 FLRYDTLLLVAGGSGITPF 1
>BQ741940 homologue to GP|18182311|gb| GT-2 factor {Glycine max}, partial
(25%)
Length = 431
Score = 31.2 bits (69), Expect = 1.5
Identities = 12/36 (33%), Positives = 17/36 (46%), Gaps = 2/36 (5%)
Frame = -2
Query: 516 NNSGEVYNWTYGVM--WYMFSFCSCICICSSVVFLW 549
N+ G++ +W YG W +C C C C LW
Sbjct: 319 NSGGDIRDWCYGTWPGWGW*CYCVCCCCCCCQSGLW 212
>BI968357
Length = 396
Score = 31.2 bits (69), Expect = 1.5
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -1
Query: 536 CSCICICSSVVFLWLKRLNKLE 557
C C+C+CS + LWL R NK +
Sbjct: 378 CVCVCVCSLSLSLWLLRGNKFK 313
>TC225801 similar to UP|Q8H179 (Q8H179) Eukaryotic initiation factor 4,
eIF4-like protein, partial (25%)
Length = 1233
Score = 31.2 bits (69), Expect = 1.5
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = -2
Query: 326 NYLSVAIKNVGSWSNKLYQELSSSSLDHL 354
+YL VA+ N GSW+ K+ Q S L+HL
Sbjct: 212 HYLEVAVLN*GSWTWKVKQSCSVQQLEHL 126
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.329 0.140 0.449
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,043,280
Number of Sequences: 63676
Number of extensions: 651345
Number of successful extensions: 5776
Number of sequences better than 10.0: 61
Number of HSP's better than 10.0 without gapping: 5523
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5692
length of query: 652
length of database: 12,639,632
effective HSP length: 103
effective length of query: 549
effective length of database: 6,081,004
effective search space: 3338471196
effective search space used: 3338471196
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 62 (28.5 bits)
Medicago: description of AC121237.9