BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121237.3 - phase: 1
(455 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC205977 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like pr... 643 0.0
TC217817 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like pr... 630 0.0
TC206561 401 e-112
TC217917 similar to UP|Q9M1L0 (Q9M1L0) Polygalacturonase-like pr... 308 4e-84
TC218721 similar to UP|Q9FJ27 (Q9FJ27) Polygalacturonase-like pr... 216 2e-70
TC209841 254 4e-68
TC218204 similar to UP|Q9FJ27 (Q9FJ27) Polygalacturonase-like pr... 244 4e-65
TC223982 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like pr... 238 4e-63
TC205976 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like pr... 222 3e-58
TC207014 weakly similar to UP|Q8LAH2 (Q8LAH2) Polygalacturonase-... 177 7e-45
BE801009 174 7e-44
TC209699 124 6e-39
BU545459 154 8e-38
CA784537 147 1e-35
TC221723 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like pr... 81 5e-31
CD395775 130 1e-30
TC233934 weakly similar to UP|Q8LAH2 (Q8LAH2) Polygalacturonase-... 115 3e-26
BU765101 107 9e-24
CA853125 similar to GP|11762132|gb| AT3g62110 {Arabidopsis thali... 104 7e-23
NP004759 polygalacturonase PG1 103 2e-22
>TC205977 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like protein,
partial (88%)
Length = 1966
Score = 643 bits (1659), Expect = 0.0
Identities = 301/442 (68%), Positives = 366/442 (82%), Gaps = 4/442 (0%)
Frame = +3
Query: 17 LNNFDYPAINCRKHSAVLTDFGGVGDGKTLNTKAFNSAITNLSQYANDGGAQLIVPPGKW 76
L+ F+Y A++CR +SA L +FGGVGDG TLNTKAF +AI NLSQYA GG+QL VPPGKW
Sbjct: 345 LDYFEYCAMSCRAYSASLEEFGGVGDGTTLNTKAFQAAIENLSQYAASGGSQLYVPPGKW 524
Query: 77 LTGSFNLTSHFTLFLQKDAVILASQYESDWPQLPALPSYGRGREKPGGRFSSLIFGTNLI 136
LTGSFNLTSHFTLFL KDAVILASQ E+DWP + LPSYGRGR+ GGRFSSLIFGTNL
Sbjct: 525 LTGSFNLTSHFTLFLHKDAVILASQDENDWPVIDPLPSYGRGRDTQGGRFSSLIFGTNLT 704
Query: 137 DVIITGNNGTIDGQGSTWWDKFQKKQLKITRPYMIEIMYSDQIQISNLTLVNSPSWFVHP 196
DVIITG+NGTIDGQG WW KF K +LK TRPY+IEIMYSD +QISNLTLVNSPSW VHP
Sbjct: 705 DVIITGDNGTIDGQGDLWWQKFHKGELKYTRPYLIEIMYSDNVQISNLTLVNSPSWNVHP 884
Query: 197 VYSSNIIINGLTILAPVDVPNTDGIDPDSSTNVLIEDNYIVSGDDCIAIKSGWDEYGIKV 256
+YSSN+++ G+TILAPV PNTDGI+PDS TN IED YIVSGDDC+A+KSGWDEYGI
Sbjct: 885 IYSSNLVVQGITILAPVTSPNTDGINPDSCTNTRIEDCYIVSGDDCVAVKSGWDEYGIAY 1064
Query: 257 GKPSQNIIVRRLTCISPKSALVALGSEMSGGIQDVRIEDVTAINTESAVRIKSAVGRGAF 316
G P++ +++RRLTCISP SA++ALGSEMSGGIQDVR ED+ AIN+ES VRIK+AVGRG +
Sbjct: 1065GMPTKQLVIRRLTCISPFSAVIALGSEMSGGIQDVRAEDIVAINSESGVRIKTAVGRGGY 1244
Query: 317 VKDIFVKGMDLNTLKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDVTAKNVTIAGKVE 376
VKDIFV+ M + T+K+ FWMTG+YG H D+ +DPNALP I INYRD+ A+NVT+A ++E
Sbjct: 1245VKDIFVRRMTMKTMKWAFWMTGNYGSHADDNYDPNALPVIQNINYRDMVAENVTMAARLE 1424
Query: 377 GISNDPFTGICVSNVTIEMSAHKKKLPWNCTDISGVTSNVVPKPCELL----KEKEIECP 432
GIS DPFTGIC+SNVTI+++ KK+PW CTDI+G++S+V P PC LL +EK C
Sbjct: 1425GISGDPFTGICISNVTIQLAKKAKKVPWTCTDIAGISSDVTPAPCGLLPDQGEEKIGACT 1604
Query: 433 FPRDKLPIENVQFKTCNFQSSV 454
FP + LPIE+++ +TC ++ ++
Sbjct: 1605FPEESLPIEDIKVQTCTYRRNL 1670
>TC217817 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like protein,
partial (92%)
Length = 1694
Score = 630 bits (1625), Expect = 0.0
Identities = 302/434 (69%), Positives = 353/434 (80%), Gaps = 4/434 (0%)
Frame = +1
Query: 19 NFDYPAINCRKHSAVLTDFGGVGDGKTLNTKAFNSAITNLSQYANDGGAQLIVPPGKWLT 78
+ +Y AINCR HSA LTDFGGVGDGKT NTKAF SAI++LSQYA++GG+QL VP GKWLT
Sbjct: 163 SLEYNAINCRAHSAALTDFGGVGDGKTSNTKAFQSAISHLSQYASEGGSQLYVPAGKWLT 342
Query: 79 GSFNLTSHFTLFLQKDAVILASQYESDWPQLPALPSYGRGREKPGGRFSSLIFGTNLIDV 138
GSF+LTSHFTL+L KDAV+LASQ ++WP L LPSYGRGR+ P GRF+SLIFGTNL DV
Sbjct: 343 GSFSLTSHFTLYLDKDAVLLASQDITEWPVLEPLPSYGRGRDAPAGRFTSLIFGTNLTDV 522
Query: 139 IITGNNGTIDGQGSTWWDKFQKKQLKITRPYMIEIMYSDQIQISNLTLVNSPSWFVHPVY 198
I+TG NGTIDGQG WW +F +K+LK TRPY+IE M+SD IQISNLTL+NSPSW VHPVY
Sbjct: 523 IVTGENGTIDGQGEFWWQQFHRKKLKYTRPYLIE*MFSDNIQISNLTLLNSPSWNVHPVY 702
Query: 199 SSNIIINGLTILAPVDVPNTDGIDPDSSTNVLIEDNYIVSGDDCIAIKSGWDEYGIKVGK 258
SSNII+ G+TI APV PNTDGI+PDS TNV IED YIVSGDDC+A+KSGWDEYGIK G
Sbjct: 703 SSNIIVQGITIYAPVTSPNTDGINPDSCTNVRIEDCYIVSGDDCVAVKSGWDEYGIKFGW 882
Query: 259 PSQNIIVRRLTCISPKSALVALGSEMSGGIQDVRIEDVTAINTESAVRIKSAVGRGAFVK 318
P++ +++RRLTCISP SA +ALGSEMSGGIQDVR ED+TAI TES VRIK+AVGRG +VK
Sbjct: 883 PTKQLVIRRLTCISPYSATIALGSEMSGGIQDVRAEDITAIQTESGVRIKTAVGRGGYVK 1062
Query: 319 DIFVKGMDLNTLKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDVTAKNVTIAGKVEGI 378
DI+VK M L+T+K+ F MTG Y H D +DPNALP+I INYRDV A+NVTIA + +GI
Sbjct: 1063DIYVKRMTLHTMKWAFKMTGDYNSHADGHYDPNALPEIKNINYRDVVAENVTIAARFQGI 1242
Query: 379 SNDPFTGICVSNVTIEMSAHKKKLPWNCTDISGVTSNVVPKPCELL----KEKEIECPFP 434
SNDPFTGIC++NVT+ M+A KK PW CTDI G+TS V P PC LL EK C FP
Sbjct: 1243SNDPFTGICIANVTLRMAAKAKKQPWTCTDIEGMTSGVTPPPCGLLPDQGPEKITACDFP 1422
Query: 435 RDKLPIENVQFKTC 448
D LPI+ V+ K C
Sbjct: 1423ADNLPIDIVELKKC 1464
>TC206561
Length = 1637
Score = 401 bits (1030), Expect = e-112
Identities = 192/394 (48%), Positives = 259/394 (65%)
Frame = +1
Query: 28 RKHSAVLTDFGGVGDGKTLNTKAFNSAITNLSQYANDGGAQLIVPPGKWLTGSFNLTSHF 87
R HS + +FG VGDGKTLNT AF +A+ +A+ GGA+L VP GKWLTGSFNLTSH
Sbjct: 31 RPHSVSILEFGAVGDGKTLNTVAFQNAVFYAKSFADKGGAKLYVPSGKWLTGSFNLTSHL 210
Query: 88 TLFLQKDAVILASQYESDWPQLPALPSYGRGREKPGGRFSSLIFGTNLIDVIITGNNGTI 147
TLFL++ A I+ASQ + W + LPSYGRG + P GR+ SLI+G NL DV+ITG+NG I
Sbjct: 211 TLFLERGATIIASQDYAHWTAMDPLPSYGRGIDVPVGRYRSLIYGQNLSDVVITGDNGII 390
Query: 148 DGQGSTWWDKFQKKQLKITRPYMIEIMYSDQIQISNLTLVNSPSWFVHPVYSSNIIINGL 207
DGQGS WWD L +RP++IE++ SD I ISNLT +NSP+W +HPVY SN+ I +
Sbjct: 391 DGQGSVWWDLISTHSLNYSRPHIIELVGSDNITISNLTFLNSPAWSIHPVYCSNVQIQKI 570
Query: 208 TILAPVDVPNTDGIDPDSSTNVLIEDNYIVSGDDCIAIKSGWDEYGIKVGKPSQNIIVRR 267
T+ AP P T GI PDSS +V I++ I +G D I +KSGWDEYG+ GKP+ N+ +R
Sbjct: 571 TVQAPTKFPYTSGIVPDSSEHVCIDNCNISTGHDAIVLKSGWDEYGVAYGKPTSNVHIRG 750
Query: 268 LTCISPKSALVALGSEMSGGIQDVRIEDVTAINTESAVRIKSAVGRGAFVKDIFVKGMDL 327
+ S A +A GSEMSGGI D+ E + N+ + +K+ GRG ++K+IF+ L
Sbjct: 751 VYLQSSSGAGLAFGSEMSGGISDIIAEQLHITNSTFGIELKTTRGRGGYMKNIFISDAKL 930
Query: 328 NTLKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDVTAKNVTIAGKVEGISNDPFTGIC 387
+ MTGS G HPD+ +DPNA+P + + + +V N+ IAG GI + PFT IC
Sbjct: 931 ENIYLGISMTGSSGSHPDDKYDPNAVPDVGNVTFENVIGANIAIAGNFSGIVDSPFTPIC 1110
Query: 388 VSNVTIEMSAHKKKLPWNCTDISGVTSNVVPKPC 421
+SNVT S+ W C+++ G++ V P+PC
Sbjct: 1111LSNVTFSTSSESSP-SWFCSNVMGISKEVFPEPC 1209
>TC217917 similar to UP|Q9M1L0 (Q9M1L0) Polygalacturonase-like protein
(AT3g42950/F18P9_110), partial (74%)
Length = 1292
Score = 308 bits (788), Expect = 4e-84
Identities = 156/336 (46%), Positives = 220/336 (65%), Gaps = 2/336 (0%)
Frame = +2
Query: 91 LQKDAVILASQYESDWPQLPALPSYGRGREKPGGRFSSLIFGTNLIDVIITGNNGTIDGQ 150
L +DAVIL E WP +P LPSYG GRE PG R+ SLI G +L DV+ITG+NGTI+GQ
Sbjct: 11 LAEDAVILGIDDEKYWPLMPPLPSYGYGREHPGPRYGSLIHGQHLKDVVITGHNGTINGQ 190
Query: 151 GSTWWDKFQKKQLKITRPYMIEIMYSDQIQISNLTLVNSPSWFVHPVYSSNIIINGLTIL 210
G TWW K+++K+L TR +++IM+S I I+N+TL +SP W +HP NI I G+TIL
Sbjct: 191 GQTWWKKYRQKRLNHTRGPLVQIMFSSDIVITNITLRDSPFWTLHPYDCKNITIKGVTIL 370
Query: 211 APV-DVPNTDGIDPDSSTNVLIEDNYIVSGDDCIAIKSGWDEYGIKVGKPSQNIIVRRLT 269
APV PNTDGIDPDS ++LIED YI GDD IAIKSGWD+YGI G+PS NI++R L
Sbjct: 371 APVFGAPNTDGIDPDSCEDMLIEDCYISVGDDAIAIKSGWDQYGIAYGRPSMNIMIRNLV 550
Query: 270 CISPKSALVALGSEMSGGIQDVRIEDVTAINTESAVRIKSAVGRGAFVKDIFVKGMDLNT 329
S SA +++GSEMSGG+ +V +E++ ++ VRIK+A GRGA+V+ I + +
Sbjct: 551 VRSMVSAGISIGSEMSGGVSNVTVENILIWDSRRGVRIKTARGRGAYVRQITYRNITFEN 730
Query: 330 LKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDVTAKNVTIAGKVEGISNDPFTGICVS 389
++ M Y +HPD+G+DP ALP + I++ V + V + ++ G P +
Sbjct: 731 VRVGIVMKTDYNEHPDDGYDPMALPILRDISFTTVHGQGVRVPVRIHGSEEIPVRNVTFQ 910
Query: 390 NVTIEMSAHKKKLPWNCTDISG-VTSNVVPKPCELL 424
++++ ++ +KKK + C + G V + P PCE L
Sbjct: 911 DMSVGLT-YKKKHIFQCAFVQGRVIGTIYPAPCENL 1015
>TC218721 similar to UP|Q9FJ27 (Q9FJ27) Polygalacturonase-like protein,
partial (53%)
Length = 1128
Score = 216 bits (550), Expect(2) = 2e-70
Identities = 102/234 (43%), Positives = 148/234 (62%)
Frame = +3
Query: 195 HPVYSSNIIINGLTILAPVDVPNTDGIDPDSSTNVLIEDNYIVSGDDCIAIKSGWDEYGI 254
H + SN+++ +TILAP D PNTDGIDPDSS+NV IED+YI +GDD +A+KSGWDEYGI
Sbjct: 207 HQDFKSNVVVRYVTILAPRDSPNTDGIDPDSSSNVCIEDSYISTGDDLVAVKSGWDEYGI 386
Query: 255 KVGKPSQNIIVRRLTCISPKSALVALGSEMSGGIQDVRIEDVTAINTESAVRIKSAVGRG 314
G+PS +I +RR+T SP + +A+GSE SGG+++V E + N + IK+ GRG
Sbjct: 387 AYGRPSSDITIRRITGSSPFAG-IAIGSETSGGVENVLAEHINLYNMGIGIHIKTNTGRG 563
Query: 315 AFVKDIFVKGMDLNTLKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDVTAKNVTIAGK 374
F+K+I + + + + ++G GDHPD+ FD NALP + G+ ++V V AG
Sbjct: 564 GFIKNITMSHVYMEEARKGIRISGDVGDHPDDKFDANALPLVKGVTIKNVWGMKVLQAGL 743
Query: 375 VEGISNDPFTGICVSNVTIEMSAHKKKLPWNCTDISGVTSNVVPKPCELLKEKE 428
++G+ N PFT IC ++ + + PW C+D+SG V P PC L +
Sbjct: 744 IQGLRNSPFTDICQYDINLHGVTGPRTPPWKCSDVSGFAHQVSPWPCSELSSNQ 905
Score = 68.6 bits (166), Expect(2) = 2e-70
Identities = 30/65 (46%), Positives = 43/65 (66%)
Frame = +1
Query: 139 IITGNNGTIDGQGSTWWDKFQKKQLKITRPYMIEIMYSDQIQISNLTLVNSPSWFVHPVY 198
I G NGTIDGQG WW+K++++ L+ TRP ++E + S I ISN+ +SP W +HP
Sbjct: 16 IFIGENGTIDGQGDEWWNKWKQRTLQFTRPNLVEFVNSRDIIISNVIFKSSPFWNIHPYR 195
Query: 199 SSNII 203
S +I
Sbjct: 196 SI*VI 210
>TC209841
Length = 1087
Score = 254 bits (650), Expect = 4e-68
Identities = 123/249 (49%), Positives = 172/249 (68%)
Frame = +1
Query: 179 IQISNLTLVNSPSWFVHPVYSSNIIINGLTILAPVDVPNTDGIDPDSSTNVLIEDNYIVS 238
+ ISN+T +NSP W +HPVY S++ I +TI+AP+ PNTDGI+PDSS NV IED YI +
Sbjct: 13 VLISNVTFMNSPFWTIHPVYCSHVTIQNVTIIAPLSSPNTDGINPDSSDNVCIEDCYIST 192
Query: 239 GDDCIAIKSGWDEYGIKVGKPSQNIIVRRLTCISPKSALVALGSEMSGGIQDVRIEDVTA 298
GDD I+IKSGWD YGI G+PS NI +RRL SA +A+GSEMSGG+ +V ED+
Sbjct: 193 GDDLISIKSGWDGYGILFGRPSTNINIRRLIG-KTTSAGIAIGSEMSGGVSEVHAEDIYI 369
Query: 299 INTESAVRIKSAVGRGAFVKDIFVKGMDLNTLKYVFWMTGSYGDHPDNGFDPNALPKISG 358
++ SA+RIK++ GRG +V+++++ M L + TG YG+HPD+ +DP+ALP I
Sbjct: 370 FDSHSAIRIKTSPGRGGYVRNVYISNMILVNVDIAIRFTGLYGEHPDDTYDPDALPVIER 549
Query: 359 INYRDVTAKNVTIAGKVEGISNDPFTGICVSNVTIEMSAHKKKLPWNCTDISGVTSNVVP 418
I +DV + V AG ++GI D F IC+SN+T+ +S KKLPWNC+ + G + V P
Sbjct: 550 ITIKDVIGEKVKRAGLIQGIKGDNFVNICLSNITLNVS---KKLPWNCSYVKGYSDLVSP 720
Query: 419 KPCELLKEK 427
+ CE L+E+
Sbjct: 721 EACEPLRER 747
>TC218204 similar to UP|Q9FJ27 (Q9FJ27) Polygalacturonase-like protein,
partial (51%)
Length = 1014
Score = 244 bits (624), Expect = 4e-65
Identities = 119/266 (44%), Positives = 165/266 (61%)
Frame = +2
Query: 163 LKITRPYMIEIMYSDQIQISNLTLVNSPSWFVHPVYSSNIIINGLTILAPVDVPNTDGID 222
L+ TRP ++E + S I ISN+ NSP W +HPVY SN+++ +TILAP D PNTDGID
Sbjct: 2 LQFTRPNLVEFVNSQDIIISNVIFKNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGID 181
Query: 223 PDSSTNVLIEDNYIVSGDDCIAIKSGWDEYGIKVGKPSQNIIVRRLTCISPKSALVALGS 282
PDSS+NV IED+YI +GDD +A+KSGWDEYGI G+PS I +RR+T SP A +A+GS
Sbjct: 182 PDSSSNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRPSYGITIRRVTGSSP-FAGIAIGS 358
Query: 283 EMSGGIQDVRIEDVTAINTESAVRIKSAVGRGAFVKDIFVKGMDLNTLKYVFWMTGSYGD 342
E SGG+++V E + N + IK+ GRG +K+I V + + + + G G
Sbjct: 359 ETSGGVENVLAEHINLFNMGVGIHIKTNSGRGGLIKNITVAHVYVENARQGIKIAGDVGG 538
Query: 343 HPDNGFDPNALPKISGINYRDVTAKNVTIAGKVEGISNDPFTGICVSNVTIEMSAHKKKL 402
HPD F+PNALP + GI ++V V AG + G+ N PFT +C+S++ +
Sbjct: 539 HPDEKFNPNALPVVKGITIKNVWGVKVNQAGLIHGLRNSPFTDVCLSDINFHGMEGPRSP 718
Query: 403 PWNCTDISGVTSNVVPKPCELLKEKE 428
W C+D+ G V P PC L +E
Sbjct: 719 SWKCSDVFGFAHQVSPWPCSQLSSQE 796
>TC223982 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like protein,
partial (31%)
Length = 445
Score = 238 bits (607), Expect = 4e-63
Identities = 113/146 (77%), Positives = 132/146 (90%)
Frame = +3
Query: 169 YMIEIMYSDQIQISNLTLVNSPSWFVHPVYSSNIIINGLTILAPVDVPNTDGIDPDSSTN 228
Y+IE+M+SD+IQISNLTL+NSPSW VHPVYSSNIII GLTI+APV PNTDGI+PDS TN
Sbjct: 3 YLIELMFSDKIQISNLTLLNSPSWNVHPVYSSNIIIKGLTIIAPVPSPNTDGINPDSCTN 182
Query: 229 VLIEDNYIVSGDDCIAIKSGWDEYGIKVGKPSQNIIVRRLTCISPKSALVALGSEMSGGI 288
IED YIVSGDDC+A+KSGWDEYGIK G P++ +++RRLTCISP+SA +ALGSEMSGGI
Sbjct: 183 TRIEDCYIVSGDDCVAVKSGWDEYGIKFGWPTKQLVIRRLTCISPESAAIALGSEMSGGI 362
Query: 289 QDVRIEDVTAINTESAVRIKSAVGRG 314
QDVR ED+TAI+TES VRIK+AVGRG
Sbjct: 363 QDVRAEDITAIHTESGVRIKTAVGRG 440
>TC205976 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like protein,
partial (32%)
Length = 689
Score = 222 bits (565), Expect = 3e-58
Identities = 99/171 (57%), Positives = 135/171 (78%), Gaps = 4/171 (2%)
Frame = +2
Query: 288 IQDVRIEDVTAINTESAVRIKSAVGRGAFVKDIFVKGMDLNTLKYVFWMTGSYGDHPDNG 347
IQD+R ED+ AINTES VRIK+AVGRG +VKDIFV+ M + T+K+ FWMTG+YG H D+
Sbjct: 2 IQDMRAEDIVAINTESGVRIKTAVGRGGYVKDIFVRRMTMKTMKWAFWMTGNYGSHADDN 181
Query: 348 FDPNALPKISGINYRDVTAKNVTIAGKVEGISNDPFTGICVSNVTIEMSAHKKKLPWNCT 407
+DPNALP I INYRD+ A+NVT+A ++EGIS DPFTGIC+SNVTI+++ KK+PW CT
Sbjct: 182 YDPNALPVIQNINYRDMVAENVTMAARLEGISGDPFTGICISNVTIQLAKKAKKVPWTCT 361
Query: 408 DISGVTSNVVPKPCELL----KEKEIECPFPRDKLPIENVQFKTCNFQSSV 454
DI+G++S+V P PC+LL +EK C FP + LPIE+++ +TC ++ ++
Sbjct: 362 DIAGISSDVTPAPCDLLPDQGEEKIGACTFPEESLPIEDIKVQTCTYRRNL 514
>TC207014 weakly similar to UP|Q8LAH2 (Q8LAH2) Polygalacturonase-like
protein, partial (66%)
Length = 1299
Score = 177 bits (450), Expect = 7e-45
Identities = 89/250 (35%), Positives = 148/250 (58%)
Frame = +1
Query: 172 EIMYSDQIQISNLTLVNSPSWFVHPVYSSNIIINGLTILAPVDVPNTDGIDPDSSTNVLI 231
+I++S +Q+ T + + ++ ++ S ++ N L +LA SS NV I
Sbjct: 127 QILHS*MLQLIAYTQFIA-AMYIFKMFQSLLLQNPLILLA*CQY---------SSDNVCI 276
Query: 232 EDNYIVSGDDCIAIKSGWDEYGIKVGKPSQNIIVRRLTCISPKSALVALGSEMSGGIQDV 291
ED + G D I++KSGWDEYGI G+P++N+ +RR+ + + +A GS+MSGGI +V
Sbjct: 277 EDCIVAMGFDAISLKSGWDEYGIAYGRPTENVHIRRVQLHAFSGSALAFGSDMSGGISNV 456
Query: 292 RIEDVTAINTESAVRIKSAVGRGAFVKDIFVKGMDLNTLKYVFWMTGSYGDHPDNGFDPN 351
+E N+ S + ++ GRG ++K+I + + + + TG+ G HPD+ FDPN
Sbjct: 457 LVEHAHLFNSNSGIEFRTTKGRGGYMKEIVMSDIQMENVHTAIAATGNCGSHPDDKFDPN 636
Query: 352 ALPKISGINYRDVTAKNVTIAGKVEGISNDPFTGICVSNVTIEMSAHKKKLPWNCTDISG 411
ALP + I +DV N+TIAG + GI PFT IC+SN+T+ ++ + W C+++SG
Sbjct: 637 ALPHLDHITLKDVIGTNITIAGNLAGIDESPFTNICLSNITLSTNS-VSPITWACSNVSG 813
Query: 412 VTSNVVPKPC 421
+ +V+P+PC
Sbjct: 814 FSDSVLPEPC 843
Score = 89.4 bits (220), Expect = 3e-18
Identities = 38/82 (46%), Positives = 56/82 (67%)
Frame = +3
Query: 142 GNNGTIDGQGSTWWDKFQKKQLKITRPYMIEIMYSDQIQISNLTLVNSPSWFVHPVYSSN 201
GNNGTIDG G WWD + L +RP+++EI+ SD + +SNLT +N+P++ +HPVY S+
Sbjct: 6 GNNGTIDGMGMVWWDWYSTHSLNHSRPHLVEIVASDYVVVSNLTFLNAPAYSIHPVYCSH 185
Query: 202 IIINGLTILAPVDVPNTDGIDP 223
+ I ++I P + P T GI P
Sbjct: 186 VHIQNVSISTPPESPYTVGIVP 251
>BE801009
Length = 425
Score = 174 bits (441), Expect = 7e-44
Identities = 82/141 (58%), Positives = 107/141 (75%), Gaps = 1/141 (0%)
Frame = +3
Query: 129 LIFGTNLIDVIITGNNGTIDGQGSTWWDKFQKKQLKITRPYMIEIMYSDQIQISNLTLVN 188
LI G NL+DV+ITG+NGTI+GQG TWW K+++K L TR +++I++S I ISN+TL +
Sbjct: 3 LIHGQNLVDVVITGHNGTINGQGQTWWTKYRQKLLNHTRGPLVQILWSSNIVISNITLRD 182
Query: 189 SPSWFVHPVYSSNIIINGLTILAPVD-VPNTDGIDPDSSTNVLIEDNYIVSGDDCIAIKS 247
SP W +HP N+ + +TILAPV PNTDGIDPDS ++LIED YI GDD IAIKS
Sbjct: 183 SPFWTLHPYDCKNVTVKKVTILAPVSHAPNTDGIDPDSCEDMLIEDCYISVGDDAIAIKS 362
Query: 248 GWDEYGIKVGKPSQNIIVRRL 268
GWD+YGI G+PS+NI++R L
Sbjct: 363 GWDQYGITYGRPSKNIVIRNL 425
>TC209699
Length = 978
Score = 124 bits (311), Expect(3) = 6e-39
Identities = 59/120 (49%), Positives = 84/120 (69%)
Frame = +2
Query: 231 IEDNYIVSGDDCIAIKSGWDEYGIKVGKPSQNIIVRRLTCISPKSALVALGSEMSGGIQD 290
IED YI +GDD IAIKSGWDEYGI G+PS NII+ RL + S +A+GSEMSGG+ +
Sbjct: 5 IEDCYISTGDDLIAIKSGWDEYGIAYGRPSTNIIIHRLVGKTQTSG-IAIGSEMSGGVSE 181
Query: 291 VRIEDVTAINTESAVRIKSAVGRGAFVKDIFVKGMDLNTLKYVFWMTGSYGDHPDNGFDP 350
V ED+ ++ +A+RIK++ GRG +V++I+V + L + TG YG+HPD+ ++P
Sbjct: 182 VHAEDIQFYDSYNAIRIKTSPGRGGYVRNIYVSNVTLANVDIAITFTGLYGEHPDDAYNP 361
Score = 43.9 bits (102), Expect(3) = 6e-39
Identities = 18/50 (36%), Positives = 31/50 (62%)
Frame = +1
Query: 378 ISNDPFTGICVSNVTIEMSAHKKKLPWNCTDISGVTSNVVPKPCELLKEK 427
+ D F IC+SN+ + ++++ PWNC+ + G + V P+ CE LKE+
Sbjct: 445 LEGDNFVNICLSNIILNVTSN---YPWNCSYVKGYSDLVQPEACEPLKER 585
Score = 31.6 bits (70), Expect(3) = 6e-39
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = +3
Query: 351 NALPKISGINYRDVTAKNVTIAGKVEGI 378
NALP I I +DV +N+ AG +EGI
Sbjct: 363 NALPVIEKITIKDVVGENIKTAGLIEGI 446
>BU545459
Length = 495
Score = 154 bits (389), Expect = 8e-38
Identities = 71/120 (59%), Positives = 90/120 (74%), Gaps = 4/120 (3%)
Frame = -1
Query: 336 MTGSYGDHPDNGFDPNALPKISGINYRDVTAKNVTIAGKVEGISNDPFTGICVSNVTIEM 395
MTG+YG H D+ +DPNALP+I+GINYRDV A NVTIA ++EGISNDPFTGIC++NVTI M
Sbjct: 495 MTGNYGSHXDSHYDPNALPEINGINYRDVVADNVTIAARLEGISNDPFTGICIANVTINM 316
Query: 396 SAHKKKLPWNCTDISGVTSNVVPKPCELL----KEKEIECPFPRDKLPIENVQFKTCNFQ 451
+A KK PW CTDI G+TS V PKPC L EK C FP++ LPI+ ++ + C ++
Sbjct: 315 AAKAKKQPWACTDIEGITSGVTPKPCNSLPDQGPEKIKACDFPQENLPIDMLELQKCVYK 136
>CA784537
Length = 434
Score = 147 bits (371), Expect = 1e-35
Identities = 72/119 (60%), Positives = 87/119 (72%)
Frame = +1
Query: 23 PAINCRKHSAVLTDFGGVGDGKTLNTKAFNSAITNLSQYANDGGAQLIVPPGKWLTGSFN 82
P ++ R HS + +FG VGDGKTLNT AF +AI L +A+ GGAQL VPPGKWLTGSFN
Sbjct: 76 PTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSFN 255
Query: 83 LTSHFTLFLQKDAVILASQYESDWPQLPALPSYGRGREKPGGRFSSLIFGTNLIDVIIT 141
LTSH TLFL+K AV++ +Q S W + LPSYGRG E PGGR+ SLI G L DV++T
Sbjct: 256 LTSHLTLFLEKGAVLIGTQDPSHWDVVEPLPSYGRGLEVPGGRYQSLINGYMLHDVVVT 432
>TC221723 similar to UP|Q84LI7 (Q84LI7) Polygalacturonase-like protein,
partial (24%)
Length = 485
Score = 80.9 bits (198), Expect(2) = 5e-31
Identities = 40/66 (60%), Positives = 47/66 (70%)
Frame = +1
Query: 66 GAQLIVPPGKWLTGSFNLTSHFTLFLQKDAVILASQYESDWPQLPALPSYGRGREKPGGR 125
GA L VP GK LTGSF+L SH TL L KD +LASQ +WP + LPSYGRGR+ GR
Sbjct: 286 GAHLYVPAGK*LTGSFSLISHLTLGLNKDTFLLASQDIREWPVIEPLPSYGRGRDAAAGR 465
Query: 126 FSSLIF 131
++SLIF
Sbjct: 466 YTSLIF 483
Score = 72.0 bits (175), Expect(2) = 5e-31
Identities = 34/48 (70%), Positives = 39/48 (80%)
Frame = +3
Query: 19 NFDYPAINCRKHSAVLTDFGGVGDGKTLNTKAFNSAITNLSQYANDGG 66
+F++ AINCR HSA L DFGGVGDG T NTKAF SAI++LSQYA GG
Sbjct: 144 SFEHKAINCRAHSASLIDFGGVGDGNTSNTKAFQSAISHLSQYAFKGG 287
>CD395775
Length = 644
Score = 130 bits (327), Expect = 1e-30
Identities = 56/144 (38%), Positives = 92/144 (63%)
Frame = -2
Query: 278 VALGSEMSGGIQDVRIEDVTAINTESAVRIKSAVGRGAFVKDIFVKGMDLNTLKYVFWMT 337
+A GS+MSGGI ++ +E+V N++S + ++ GRG ++K+I + +++ + T
Sbjct: 640 IAFGSDMSGGISNILVENVHLYNSKSGIEFRTMRGRGGYMKEIIISDIEMENIYTAIAAT 461
Query: 338 GSYGDHPDNGFDPNALPKISGINYRDVTAKNVTIAGKVEGISNDPFTGICVSNVTIEMSA 397
G G HPD+ FDPNALP + I +D+ N+TIAG G+ PFT IC+SN+T+ ++
Sbjct: 460 GYCGSHPDDKFDPNALPLLDHIILQDMIGTNITIAGSFAGLQESPFTNICLSNITLSTNS 281
Query: 398 HKKKLPWNCTDISGVTSNVVPKPC 421
+PW C+++SG + V+PKPC
Sbjct: 280 -VSSIPWECSNVSGFSDYVLPKPC 212
>TC233934 weakly similar to UP|Q8LAH2 (Q8LAH2) Polygalacturonase-like
protein, partial (27%)
Length = 911
Score = 115 bits (289), Expect = 3e-26
Identities = 53/156 (33%), Positives = 92/156 (58%)
Frame = +2
Query: 278 VALGSEMSGGIQDVRIEDVTAINTESAVRIKSAVGRGAFVKDIFVKGMDLNTLKYVFWMT 337
+A GSEMSGGI + E + +N+ + +K+ GRG +++ IF+ +L + MT
Sbjct: 65 LAFGSEMSGGISVIIAEKLHILNSPIGIELKTTRGRGGYMRGIFISDAELENISLGVSMT 244
Query: 338 GSYGDHPDNGFDPNALPKISGINYRDVTAKNVTIAGKVEGISNDPFTGICVSNVTIEMSA 397
G G HPD+ +D ++LP + I +++V N+++AG GI PF+ IC+SNVT +S+
Sbjct: 245 GYSGFHPDDKYDTSSLPVVGDITFKNVIGANISVAGNFSGIVESPFSTICLSNVTFSLSS 424
Query: 398 HKKKLPWNCTDISGVTSNVVPKPCELLKEKEIECPF 433
W C+++ G + +V+P+PC ++ + PF
Sbjct: 425 EPSP-SWFCSNVIGFSEHVIPEPCPDIQSSYSKFPF 529
>BU765101
Length = 423
Score = 107 bits (268), Expect = 9e-24
Identities = 52/78 (66%), Positives = 58/78 (73%)
Frame = +1
Query: 65 GGAQLIVPPGKWLTGSFNLTSHFTLFLQKDAVILASQYESDWPQLPALPSYGRGREKPGG 124
GGAQL VPPG+WLT FNLTSH TLFL +DAVIL E WP +P LPSYG GRE PG
Sbjct: 1 GGAQLNVPPGRWLTAPFNLTSHMTLFLAEDAVILGIDDEKYWPLMPPLPSYGYGREHPGP 180
Query: 125 RFSSLIFGTNLIDVIITG 142
R+ SLI G +L DV+ITG
Sbjct: 181 RYGSLIHGQHLKDVVITG 234
>CA853125 similar to GP|11762132|gb| AT3g62110 {Arabidopsis thaliana},
partial (17%)
Length = 546
Score = 104 bits (260), Expect = 7e-23
Identities = 50/80 (62%), Positives = 59/80 (73%)
Frame = +3
Query: 28 RKHSAVLTDFGGVGDGKTLNTKAFNSAITNLSQYANDGGAQLIVPPGKWLTGSFNLTSHF 87
R HS +T+FG VGDG TLNTKAF +AI L+ +A+ GGA+L VP G+WLTGSF+L SH
Sbjct: 279 RPHSVSITEFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHL 458
Query: 88 TLFLQKDAVILASQYESDWP 107
TL L KDAVIL S DWP
Sbjct: 459 TLSLDKDAVILGSTNPEDWP 518
>NP004759 polygalacturonase PG1
Length = 1332
Score = 103 bits (256), Expect = 2e-22
Identities = 100/410 (24%), Positives = 174/410 (42%), Gaps = 20/410 (4%)
Frame = +1
Query: 36 DFGGVGDGKTLNTKAFNSAITNLSQYANDGGAQLIVPPGK-WLTGSFNLTS---HFTLFL 91
D+G GDG +TKAF A + G+ ++VP G +L + + +
Sbjct: 148 DYGAKGDGHADDTKAFEDAWAAACKVE---GSTMVVPSGSVFLVKPISFSGPNCEPNIVF 318
Query: 92 QKDAVILASQYESDWPQLPALPSYGRGREKPGGRFSSLIFGTNLIDVIITGNNGTIDGQG 151
Q D I+A W G L F ++ I G IDGQG
Sbjct: 319 QLDGKIIAPTSSEAWGS---------------GTLQWLEFSK--LNTITIRGKGVIDGQG 447
Query: 152 STWWDKFQKK------------QLKITRPYMIEIMYSDQIQISNLTLVNSPSWFVHPVYS 199
S WW+ +L T+P + SD + ++ +T+ NS +
Sbjct: 448 SVWWNNDSPTYNPTEVMLESNGRLPSTKPTALRFYGSDGVTVTGITIQNSQQTHLKFDSC 627
Query: 200 SNIIINGLTILAPVDVPNTDGIDPDSSTNVLIEDNYIVSGDDCIAIKSGWDEYGIKVGKP 259
+N+ ++G+++ +P D PNTDGI +S NV+I + + GDDC++I++G
Sbjct: 628 TNVQVSGISVSSPGDSPNTDGIHLQNSQNVVIYSSTLACGDDCVSIQTG----------- 774
Query: 260 SQNIIVRRLTCISPKS-ALVALGSEMSGG-IQDVRIEDVTAINTESAVRIKSAVGRGAFV 317
+I V + C ++ +LG E + +++V + DVT NT + VRIK+ G V
Sbjct: 775 CSDIYVHNVNCGPGHGISIGSLGRENTKACVRNVTVRDVTIQNTLTGVRIKTWQGGSGSV 954
Query: 318 KDIFVKGMDLNTLKYVFWMTGSYGDHPDNGFDPNALPKISGINYRDVTAKNVTIAGKVEG 377
++I + ++ ++ + Y D + +A+ +SGI+Y +V
Sbjct: 955 QNIMFSNVQVSGVQTPISIDQYYCDGGRCRNESSAV-AVSGIHYVNVKGTYTKEPIYFAC 1131
Query: 378 ISNDPFTGICVSNVTIEMSAHKK--KLPWNCTDISGVTSNVVPKPCELLK 425
N P +GI + + +E + K +P+ C + G P E L+
Sbjct: 1132SDNLPCSGITLDTIQLESAQETKNSNVPF-CWEAYGELKTTTVPPVECLQ 1278
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.137 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,450,850
Number of Sequences: 63676
Number of extensions: 250544
Number of successful extensions: 1252
Number of sequences better than 10.0: 66
Number of HSP's better than 10.0 without gapping: 1217
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1224
length of query: 455
length of database: 12,639,632
effective HSP length: 100
effective length of query: 355
effective length of database: 6,272,032
effective search space: 2226571360
effective search space used: 2226571360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 61 (28.1 bits)
Medicago: description of AC121237.3