BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC119417.4 - phase: 0 /pseudo
(459 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CO979507 197 6e-51
TC229557 weakly similar to UP|Q9FUJ1 (Q9FUJ1) Cytokinin oxidase ... 193 1e-49
TC220263 similar to UP|Q9FUJ0 (Q9FUJ0) Cytokinin oxidase, partia... 135 2e-35
BI970438 weakly similar to SP|Q9LTS3|CKX3 Cytokinin oxidase 3 pr... 130 9e-31
TC222766 similar to UP|Q9FUJ0 (Q9FUJ0) Cytokinin oxidase, partia... 106 2e-23
TC234116 similar to UP|Q9LY71 (Q9LY71) Cytokinin oxidase-like pr... 101 8e-22
TC220851 similar to UP|Q84U27 (Q84U27) Cytokinin dehydrogenase 3... 92 5e-19
BG651837 weakly similar to GP|11120514|gb| cytokinin oxidase {Ar... 89 3e-18
CA936072 weakly similar to GP|11120514|gb| cytokinin oxidase {Ar... 79 4e-15
BI498566 similar to GP|11120514|gb cytokinin oxidase {Arabidopsi... 74 1e-13
TC232674 similar to UP|Q9FUJ1 (Q9FUJ1) Cytokinin oxidase (FAD-li... 55 5e-08
TC204813 similar to UP|RSP4_CICAR (O65751) 40S ribosomal protein... 30 2.3
TC204376 similar to UP|Q9SVG3 (Q9SVG3) Reticuline oxidase-like p... 29 5.0
AW308995 28 8.6
BG510985 similar to GP|20279572|gb L-galactono-1 4-lactone dehyd... 28 8.6
TC204812 similar to UP|RSP4_CICAR (O65751) 40S ribosomal protein... 28 8.6
>CO979507
Length = 593
Score = 197 bits (502), Expect = 6e-51
Identities = 94/181 (51%), Positives = 130/181 (70%), Gaps = 2/181 (1%)
Frame = -1
Query: 251 LFCLELAKYFNMEETLEVNQDIQKHLSHLNFIPSTLFQTEVTYVDFLDRVHISEVKLRSK 310
L+CLE+AKY++ + V++++Q L L++IP ++ +V+Y +FL+RV E+KL+S+
Sbjct: 542 LYCLEVAKYYDGQNENNVDKELQVLLQGLSYIPGFYYEKDVSYFEFLNRVRSGELKLQSQ 363
Query: 311 GLWDVPHPWLNLFIPKSKIHNFAEVVFGNIV--KETSNGPVLIYPVHKSKWDKRTSVVIP 368
GLWDVPHPWLNLFIPKS+I F VF NI+ + + GPVL+YP++++KWD R S IP
Sbjct: 362 GLWDVPHPWLNLFIPKSQIMEFDSGVFKNIILKRNITTGPVLVYPMNRNKWDNRMSASIP 183
Query: 369 DEDIFYLVGFLASSSGPDELEHILSQNKRILEYCERAHLGVKQYLPHYTTQEEWQTHYGH 428
DEDIFY VGFL SSG D + +QNK IL++C A + VKQY PHY TQE+W H+G
Sbjct: 182 DEDIFYTVGFL-HSSGFDNWKAYDAQNKEILQFCNVAGIKVKQYFPHYCTQEDWANHFGP 6
Query: 429 K 429
K
Sbjct: 5 K 3
>TC229557 weakly similar to UP|Q9FUJ1 (Q9FUJ1) Cytokinin oxidase (FAD-linked
oxidoreductase family), partial (13%)
Length = 841
Score = 193 bits (491), Expect = 1e-49
Identities = 93/211 (44%), Positives = 130/211 (61%), Gaps = 4/211 (1%)
Frame = +1
Query: 251 LFCLELAKYF-NMEETLEVNQDIQKHLSHLNFIPSTLFQTEVTYVDFLDRVHISEVKLRS 309
L+CLELA ++ N + V+ ++ + L L F+ F +VTY++FL RV E ++
Sbjct: 4 LYCLELALHYRNQDHPSAVDMEVDRLLGRLRFVEGLKFCVDVTYMEFLLRVKRVEEDAKA 183
Query: 310 KGLWDVPHPWLNLFIPKSKIHNFAEVVFGNIVKETSNGPVLIYPVHKSKWDKRTSVVIPD 369
G+WD PHPWLN+F+ KS I F VF I+K GP+L+YP+ +SKWD R SVV+PD
Sbjct: 184 NGIWDAPHPWLNMFVSKSNIAEFDREVFKKILKHGVGGPILVYPLLRSKWDSRHSVVVPD 363
Query: 370 EDIFYLVGFLASSSGPDE---LEHILSQNKRILEYCERAHLGVKQYLPHYTTQEEWQTHY 426
+IFY++ L P + E ++ QN I++ C K YLPHY +QE W HY
Sbjct: 364 SNIFYIIALLRFIPPPPKGPPTELLVEQNHEIIQLCYNRGFDFKLYLPHYQSQENWMRHY 543
Query: 427 GHKWEIFKQRKSIYDPLAILAPGQGIFSKSI 457
G KW F +RK+ +DPLAILAPGQ IF +S+
Sbjct: 544 GDKWTRFVERKANFDPLAILAPGQKIFPESL 636
>TC220263 similar to UP|Q9FUJ0 (Q9FUJ0) Cytokinin oxidase, partial (28%)
Length = 693
Score = 135 bits (340), Expect(2) = 2e-35
Identities = 71/149 (47%), Positives = 102/149 (67%), Gaps = 6/149 (4%)
Frame = +1
Query: 149 SEEQNGELFYSVLGGLGQFGIITRARILLEPAPTMVKWIRVLYSDFTAFTKDQEKLIF-- 206
S ++N ELF++VLGGLGQFG+I RARI LEPAP VKW+R+LYSDF AFTKDQE+LI
Sbjct: 250 SSQKNLELFHAVLGGLGQFGVIARARIALEPAPKRVKWVRLLYSDFFAFTKDQERLISIN 429
Query: 207 ---AEKAFDYIEGFVIKNRTGLLNNWRLSFNP-QDPVQASKFKSDGRTLFCLELAKYFNM 262
+ A D++EG ++ N+ G +NNWR SF P D + + ++ L+CLE+AKY++
Sbjct: 430 GRKQKNALDFLEGMLLMNQ-GPINNWRSSFFPLSDHPRIASLITEHSILYCLEVAKYYDE 606
Query: 263 EETLEVNQDIQKHLSHLNFIPSTLFQTEV 291
+ L V+++I+ L L +IP ++ V
Sbjct: 607 QTELNVDKEIEVLLQGLAYIPGFYYEKNV 693
Score = 32.3 bits (72), Expect(2) = 2e-35
Identities = 23/61 (37%), Positives = 29/61 (46%)
Frame = +2
Query: 84 MWMCQEVNYG*RFCMRH*SMVWHQDLGQIICI*QLVVLYPMLVLVVRLLGMVHRSVMFRR 143
M M + N G + H +M HQ LG IIC * +PML V R M +S F +
Sbjct: 32 MRMLEGNNSGLMCYIPHLNMDLHQFLGLIICT*PWEGHFPMLESVARASVMDLKSATFMK 211
Query: 144 W 144
W
Sbjct: 212 W 214
>BI970438 weakly similar to SP|Q9LTS3|CKX3 Cytokinin oxidase 3 precursor (EC
1.4.3.-) (CKO 3). [Mouse-ear cress] {Arabidopsis
thaliana}, partial (26%)
Length = 773
Score = 130 bits (328), Expect = 9e-31
Identities = 68/140 (48%), Positives = 91/140 (64%), Gaps = 2/140 (1%)
Frame = -3
Query: 320 LNLFIPKSKIHNFAEVVFGNIV--KETSNGPVLIYPVHKSKWDKRTSVVIPDEDIFYLVG 377
LN P S+I VF +IV + GPVL+YP++++KW R S IPDED+FY VG
Sbjct: 771 LNCXXPXSQILXXNSGVFKDIVLKRNXXXGPVLVYPMNRNKWYDRMSASIPDEDVFYTVG 592
Query: 378 FLASSSGPDELEHILSQNKRILEYCERAHLGVKQYLPHYTTQEEWQTHYGHKWEIFKQRK 437
FL SSG D + +QN+ ILE+C A + VKQYLP+++TQE+W H+G KW F +RK
Sbjct: 591 FL-HSSGFDTWKAYDAQNREILEFCRDAGIMVKQYLPNHSTQEDWTNHFGAKWMKFLERK 415
Query: 438 SIYDPLAILAPGQGIFSKSI 457
+DP IL+PGQ IF K +
Sbjct: 414 HQFDPRMILSPGQKIFHKKL 355
>TC222766 similar to UP|Q9FUJ0 (Q9FUJ0) Cytokinin oxidase, partial (16%)
Length = 276
Score = 106 bits (264), Expect = 2e-23
Identities = 48/84 (57%), Positives = 67/84 (79%)
Frame = -3
Query: 268 VNQDIQKHLSHLNFIPSTLFQTEVTYVDFLDRVHISEVKLRSKGLWDVPHPWLNLFIPKS 327
V Q+IQ L LNFIP+++F T++ +VD+LDRVH +E+KL+SKGLWDV HPWLNLF+PKS
Sbjct: 253 VMQEIQALLKKLNFIPTSVFTTDLPHVDYLDRVHKAELKLKSKGLWDVHHPWLNLFVPKS 74
Query: 328 KIHNFAEVVFGNIVKETSNGPVLI 351
+I +F + VF I+ ++GP+LI
Sbjct: 73 RIEDFDKGVFKGILGNKTSGPILI 2
>TC234116 similar to UP|Q9LY71 (Q9LY71) Cytokinin oxidase-like protein,
partial (10%)
Length = 638
Score = 101 bits (251), Expect = 8e-22
Identities = 42/70 (60%), Positives = 56/70 (80%)
Frame = +1
Query: 389 EHILSQNKRILEYCERAHLGVKQYLPHYTTQEEWQTHYGHKWEIFKQRKSIYDPLAILAP 448
EHIL+QNKR++++ A + VKQYLPHY+TQEEWQ H+G +WE F +RK YDPLA+LAP
Sbjct: 10 EHILAQNKRVIDFYTGAKMRVKQYLPHYSTQEEWQVHFGSRWETFVERKRAYDPLALLAP 189
Query: 449 GQGIFSKSIT 458
G IF K+++
Sbjct: 190 GHKIFQKAVS 219
>TC220851 similar to UP|Q84U27 (Q84U27) Cytokinin dehydrogenase 3 , partial
(9%)
Length = 651
Score = 92.0 bits (227), Expect = 5e-19
Identities = 40/55 (72%), Positives = 46/55 (82%)
Frame = +2
Query: 405 AHLGVKQYLPHYTTQEEWQTHYGHKWEIFKQRKSIYDPLAILAPGQGIFSKSITF 459
A LGVKQYLPHY TQ+EW+ H+G +WE F RKS+YDPLAILAPGQ IF K+ITF
Sbjct: 5 AQLGVKQYLPHYNTQQEWRAHFGPQWETFLLRKSVYDPLAILAPGQRIFQKAITF 169
>BG651837 weakly similar to GP|11120514|gb| cytokinin oxidase {Arabidopsis
thaliana}, partial (15%)
Length = 414
Score = 89.4 bits (220), Expect = 3e-18
Identities = 42/101 (41%), Positives = 60/101 (58%)
Frame = +2
Query: 259 YFNMEETLEVNQDIQKHLSHLNFIPSTLFQTEVTYVDFLDRVHISEVKLRSKGLWDVPHP 318
Y N + V+ D+ L L FI FQ +VTY++FL RV E + G WD PHP
Sbjct: 14 YRNADHPSRVDTDVDGLLGRLRFIQGLKFQVDVTYMEFLLRVKRVEEHAKGNGTWDAPHP 193
Query: 319 WLNLFIPKSKIHNFAEVVFGNIVKETSNGPVLIYPVHKSKW 359
WLNLF+ KS I +F VF I+K+ +GP+L+YP+ ++ +
Sbjct: 194 WLNLFVSKSHIVDFDREVFKKILKDGVDGPILVYPLLRNNY 316
>CA936072 weakly similar to GP|11120514|gb| cytokinin oxidase {Arabidopsis
thaliana}, partial (12%)
Length = 421
Score = 79.0 bits (193), Expect = 4e-15
Identities = 37/84 (44%), Positives = 49/84 (58%)
Frame = +1
Query: 372 IFYLVGFLASSSGPDELEHILSQNKRILEYCERAHLGVKQYLPHYTTQEEWQTHYGHKWE 431
I L+ F+ E ++ QN I++ C K YLPHY ++E W HYG KW
Sbjct: 4 IIALLRFIPPPPKGPPTELLVEQNHEIIQLCYNRGFDFKLYLPHYQSKENWMRHYGDKWS 183
Query: 432 IFKQRKSIYDPLAILAPGQGIFSK 455
F +RK+ +DPLAILAPGQ IFS+
Sbjct: 184 RFVERKANFDPLAILAPGQKIFSR 255
>BI498566 similar to GP|11120514|gb cytokinin oxidase {Arabidopsis thaliana},
partial (22%)
Length = 378
Score = 73.9 bits (180), Expect = 1e-13
Identities = 34/49 (69%), Positives = 42/49 (85%)
Frame = +3
Query: 146 LLQSEEQNGELFYSVLGGLGQFGIITRARILLEPAPTMVKWIRVLYSDF 194
L+ SE QN ELF++ LGGLGQFGIITRAR+ ++ AP MV+WIRVLYS+F
Sbjct: 228 LVCSESQNSELFFATLGGLGQFGIITRARLPVQQAPDMVRWIRVLYSEF 374
>TC232674 similar to UP|Q9FUJ1 (Q9FUJ1) Cytokinin oxidase (FAD-linked
oxidoreductase family), partial (28%)
Length = 779
Score = 55.5 bits (132), Expect = 5e-08
Identities = 26/39 (66%), Positives = 32/39 (81%)
Frame = +2
Query: 146 LLQSEEQNGELFYSVLGGLGQFGIITRARILLEPAPTMV 184
L+ SE QN ELF++ LGGLGQFGIITRAR+ ++ AP MV
Sbjct: 620 LVCSESQNSELFFATLGGLGQFGIITRARVPVQQAPDMV 736
>TC204813 similar to UP|RSP4_CICAR (O65751) 40S ribosomal protein SA (p40),
partial (84%)
Length = 1206
Score = 30.0 bits (66), Expect = 2.3
Identities = 17/40 (42%), Positives = 25/40 (62%)
Frame = -1
Query: 97 CMRH*SMVWHQDLGQIICI*QLVVLYPMLVLVVRLLGMVH 136
C RH*S+ WH +LGQ++ LV +P+ VL R+ +H
Sbjct: 801 CQRH*SLKWH-NLGQVVLHLPLVA-WPLQVLYRRISPPLH 688
>TC204376 similar to UP|Q9SVG3 (Q9SVG3) Reticuline oxidase-like protein,
partial (63%)
Length = 1418
Score = 28.9 bits (63), Expect = 5.0
Identities = 17/45 (37%), Positives = 23/45 (50%), Gaps = 1/45 (2%)
Frame = +1
Query: 146 LLQSEEQNGELFYSVLGGLG-QFGIITRARILLEPAPTMVKWIRV 189
LL + +LF+++ GG G FG+I I L P P V RV
Sbjct: 307 LLNRKTMGEDLFWAIRGGGGASFGVILSFTIKLVPVPETVTGFRV 441
>AW308995
Length = 458
Score = 28.1 bits (61), Expect = 8.6
Identities = 14/36 (38%), Positives = 22/36 (60%), Gaps = 1/36 (2%)
Frame = +2
Query: 155 ELFYSVLGGLG-QFGIITRARILLEPAPTMVKWIRV 189
+LF+++ GG G FG++ +I L P P +V RV
Sbjct: 11 DLFWAIRGGGGASFGVVVSWKIRLVPVPEVVTVFRV 118
>BG510985 similar to GP|20279572|gb L-galactono-1 4-lactone dehydrogenase
{Cucumis melo}, partial (20%)
Length = 371
Score = 28.1 bits (61), Expect = 8.6
Identities = 22/94 (23%), Positives = 40/94 (42%), Gaps = 6/94 (6%)
Frame = +2
Query: 149 SEEQNGELFYSVLGGLGQFGIITRARILLEPAPTMVKWIRVLYSDFTAFTKDQEKLIFAE 208
S++++ ELFY GLG G++ + +V+ + S K+ +KL+
Sbjct: 86 SKDKDPELFYLARCGLGGLGVVAEVTLQCVDRQELVE--HTVVSTMNEIKKNHKKLLSEN 259
Query: 209 KAFDYI----EGFVIKNRTGLLNNWR--LSFNPQ 236
K Y+ V+ R ++ W+ F PQ
Sbjct: 260 KHVKYLYIPYTDSVVVVRCNPVSKWKGPPKFKPQ 361
>TC204812 similar to UP|RSP4_CICAR (O65751) 40S ribosomal protein SA (p40),
partial (84%)
Length = 1236
Score = 28.1 bits (61), Expect = 8.6
Identities = 16/40 (40%), Positives = 25/40 (62%)
Frame = -3
Query: 97 CMRH*SMVWHQDLGQIICI*QLVVLYPMLVLVVRLLGMVH 136
C +H*S+ WH +LGQ++ LV +P+ VL R+ +H
Sbjct: 799 CQQH*SLKWH-NLGQVVLHLPLVA-WPLQVLYRRINPPLH 686
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.336 0.148 0.478
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,179,875
Number of Sequences: 63676
Number of extensions: 329029
Number of successful extensions: 2574
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 2516
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2568
length of query: 459
length of database: 12,639,632
effective HSP length: 101
effective length of query: 358
effective length of database: 6,208,356
effective search space: 2222591448
effective search space used: 2222591448
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC119417.4