BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0592.7
(116 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from tran... 113 7e-26
COPI_DROME (P04146) Copia protein (Gag-int-pol protein) [Contain... 101 3e-22
YCH4_YEAST (P25600) Transposon Ty5-1 34.5 kDa hypothetical protein 49 2e-06
YMU0_YEAST (Q04670) Transposon Ty1 protein B 45 2e-05
YJL3_YEAST (P47024) Transposon Ty4 207.7 kDa hypothetical protein 45 3e-05
YMT5_YEAST (Q04214) Transposon Ty1 protein B 42 2e-04
YME4_YEAST (Q04711) Transposon Ty1 protein B 42 2e-04
YMD9_YEAST (Q03434) Transposon Ty1 protein B 42 2e-04
YJZ9_YEAST (P47100) Transposon Ty1 protein B 42 2e-04
YCB9_YEAST (P25384) Transposon Ty2 protein B (Ty1-17 protein B) 41 6e-04
YJZ7_YEAST (P47098) Transposon Ty1 protein B 40 0.001
M820_ARATH (P92520) Hypothetical mitochondrial protein AtMg00820... 34 0.052
GLCE_HUMAN (O94923) D-glucuronyl C5-epimerase (EC 5.1.3.-) (Hepa... 33 0.089
GLCE_BOVIN (O18756) D-glucuronyl C5 epimerase (EC 5.1.3.-) (Hepa... 33 0.089
GLCE_MOUSE (Q9EPS3) D-glucuronyl C5-epimerase (EC 5.1.3.-) (Hepa... 31 0.44
GSA_PYRAE (Q8ZYW1) Probable glutamate-1-semialdehyde 2,1-aminomu... 30 1.3
SYE2_RICCN (Q92H06) Glutamyl-tRNA synthetase 2 (EC 6.1.1.17) (Gl... 29 2.2
PYRG_WIGBR (Q8D2K0) CTP synthase (EC 6.3.4.2) (UTP--ammonia liga... 28 2.9
TOP1_BUCAI (P57371) DNA topoisomerase I (EC 5.99.1.2) (Omega-pro... 28 3.7
GRSB_BACBR (P14688) Gramicidin S synthetase II [Includes: ATP-de... 28 4.9
>POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from
transposon TNT 1-94 [Contains: Protease (EC 3.4.23.-);
Reverse transcriptase (EC 2.7.7.49); Endonuclease]
Length = 1328
Score = 113 bits (283), Expect = 7e-26
Identities = 53/111 (47%), Positives = 78/111 (69%), Gaps = 1/111 (0%)
Query: 6 FTQKEGIGYFDTYAPVARITTIRVLLALASLYKFVIHQMDVKTAFLNGELDEEVYMKQPE 65
F QK+GI + + ++PV ++T+IR +L+LA+ + Q+DVKTAFL+G+L+EE+YM+QPE
Sbjct: 883 FEQKKGIDFDEIFSPVVKMTSIRTILSLAASLDLEVEQLDVKTAFLHGDLEEEIYMEQPE 942
Query: 66 GFVIKGQVQKVCKLTKSLYELKQTPKKKWHQKFDQVVLAHEYKINEFDKCV 116
GF + G+ VCKL KSLY LKQ P ++W+ KFD + + Y D CV
Sbjct: 943 GFEVAGKKHMVCKLNKSLYGLKQAP-RQWYMKFDSFMKSQTYLKTYSDPCV 992
>COPI_DROME (P04146) Copia protein (Gag-int-pol protein) [Contains:
Copia VLP protein; Copia protease (EC 3.4.23.-)]
Length = 1409
Score = 101 bits (251), Expect = 3e-22
Identities = 52/111 (46%), Positives = 72/111 (64%), Gaps = 3/111 (2%)
Query: 6 FTQKEGIGYFDTYAPVARITTIRVLLALASLYKFVIHQMDVKTAFLNGELDEEVYMKQPE 65
FTQK I Y +T+APVARI++ R +L+L Y +HQMDVKTAFLNG L EE+YM+ P+
Sbjct: 963 FTQKYQIDYEETFAPVARISSFRFILSLVIQYNLKVHQMDVKTAFLNGTLKEEIYMRLPQ 1022
Query: 66 GFVIKGQVQKVCKLTKSLYELKQTPKKKWHQKFDQVVLAHEYKINEFDKCV 116
G I VCKL K++Y LKQ + W + F+Q + E+ + D+C+
Sbjct: 1023 G--ISCNSDNVCKLNKAIYGLKQA-ARCWFEVFEQALKECEFVNSSVDRCI 1070
>YCH4_YEAST (P25600) Transposon Ty5-1 34.5 kDa hypothetical
protein
Length = 308
Score = 49.3 bits (116), Expect = 2e-06
Identities = 24/47 (51%), Positives = 30/47 (63%)
Query: 44 MDVKTAFLNGELDEEVYMKQPEGFVIKGQVQKVCKLTKSLYELKQTP 90
MDV TAFLN +DE +Y+KQP GFV + V +L +Y LKQ P
Sbjct: 1 MDVDTAFLNSTMDEPIYVKQPPGFVNERNPDYVWELYGGMYGLKQAP 47
>YMU0_YEAST (Q04670) Transposon Ty1 protein B
Length = 1328
Score = 45.4 bits (106), Expect = 2e-05
Identities = 29/89 (32%), Positives = 47/89 (52%), Gaps = 8/89 (8%)
Query: 6 FTQKEGIGYFDTYAPVARITTIR-----VLLALASLYKFVIHQMDVKTAFLNGELDEEVY 60
F + I + DTY P + T+ L+LA + I Q+D+ +A+L ++ EE+Y
Sbjct: 875 FVARGDIQHPDTYDPGMQSNTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELY 934
Query: 61 MKQPEGFVIKGQVQKVCKLTKSLYELKQT 89
++ P G K+ +L KSLY LKQ+
Sbjct: 935 IRPPPHL---GMNDKLIRLKKSLYGLKQS 960
>YJL3_YEAST (P47024) Transposon Ty4 207.7 kDa hypothetical protein
Length = 1803
Score = 45.1 bits (105), Expect = 3e-05
Identities = 30/101 (29%), Positives = 51/101 (49%), Gaps = 11/101 (10%)
Query: 16 DTYAPVARITT----IRVLLALASLYKFVIHQMDVKTAFLNGELDEEVYMKQPEGFVIKG 71
DTY+ + + I++ L +A+ + +D+ AFL +L+EE+Y+ P
Sbjct: 1351 DTYSVITTESLNHNHIKIFLMIANNRNMFMKTLDINHAFLYAKLEEEIYIPHPH------ 1404
Query: 72 QVQKVCKLTKSLYELKQTPKKKWHQKFDQVVLAHEYKINEF 112
+ V KL K+LY LKQ+P K+W+ Q + K N +
Sbjct: 1405 DRRCVVKLNKALYGLKQSP-KEWNDHLRQYLNGIGLKDNSY 1444
>YMT5_YEAST (Q04214) Transposon Ty1 protein B
Length = 1328
Score = 42.4 bits (98), Expect = 2e-04
Identities = 28/89 (31%), Positives = 46/89 (51%), Gaps = 8/89 (8%)
Query: 6 FTQKEGIGYFDTYAPVARITTIR-----VLLALASLYKFVIHQMDVKTAFLNGELDEEVY 60
F + I + DTY + T+ L+LA + I Q+D+ +A+L ++ EE+Y
Sbjct: 875 FVARGDIQHPDTYDSGMQSNTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELY 934
Query: 61 MKQPEGFVIKGQVQKVCKLTKSLYELKQT 89
++ P G K+ +L KSLY LKQ+
Sbjct: 935 IRPPPHL---GMNDKLIRLKKSLYGLKQS 960
>YME4_YEAST (Q04711) Transposon Ty1 protein B
Length = 1328
Score = 42.4 bits (98), Expect = 2e-04
Identities = 28/89 (31%), Positives = 46/89 (51%), Gaps = 8/89 (8%)
Query: 6 FTQKEGIGYFDTYAPVARITTIR-----VLLALASLYKFVIHQMDVKTAFLNGELDEEVY 60
F + I + DTY + T+ L+LA + I Q+D+ +A+L ++ EE+Y
Sbjct: 875 FVARGDIQHPDTYDSGMQSNTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELY 934
Query: 61 MKQPEGFVIKGQVQKVCKLTKSLYELKQT 89
++ P G K+ +L KSLY LKQ+
Sbjct: 935 IRPPPHL---GMNDKLIRLKKSLYGLKQS 960
>YMD9_YEAST (Q03434) Transposon Ty1 protein B
Length = 1328
Score = 42.4 bits (98), Expect = 2e-04
Identities = 28/89 (31%), Positives = 46/89 (51%), Gaps = 8/89 (8%)
Query: 6 FTQKEGIGYFDTYAPVARITTIR-----VLLALASLYKFVIHQMDVKTAFLNGELDEEVY 60
F + I + DTY + T+ L+LA + I Q+D+ +A+L ++ EE+Y
Sbjct: 875 FVARGDIQHPDTYDSGMQSNTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELY 934
Query: 61 MKQPEGFVIKGQVQKVCKLTKSLYELKQT 89
++ P G K+ +L KSLY LKQ+
Sbjct: 935 IRPPPHL---GMNDKLIRLKKSLYGLKQS 960
>YJZ9_YEAST (P47100) Transposon Ty1 protein B
Length = 1755
Score = 42.4 bits (98), Expect = 2e-04
Identities = 28/89 (31%), Positives = 46/89 (51%), Gaps = 8/89 (8%)
Query: 6 FTQKEGIGYFDTYAPVARITTIR-----VLLALASLYKFVIHQMDVKTAFLNGELDEEVY 60
F + I + DTY + T+ L+LA + I Q+D+ +A+L ++ EE+Y
Sbjct: 1302 FVARGDIQHPDTYDSGMQSNTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELY 1361
Query: 61 MKQPEGFVIKGQVQKVCKLTKSLYELKQT 89
++ P G K+ +L KSLY LKQ+
Sbjct: 1362 IRPPPHL---GMNDKLIRLKKSLYGLKQS 1387
>YCB9_YEAST (P25384) Transposon Ty2 protein B (Ty1-17 protein B)
Length = 1770
Score = 40.8 bits (94), Expect = 6e-04
Identities = 27/89 (30%), Positives = 45/89 (50%), Gaps = 8/89 (8%)
Query: 6 FTQKEGIGYFDTYAPVARITTIRVLLALASLY-----KFVIHQMDVKTAFLNGELDEEVY 60
F + I + DTY + T+ + SL + I Q+D+ +A+L ++ EE+Y
Sbjct: 1317 FVARGDIQHPDTYDSDMQSNTVHHYALMTSLSIALDNDYYITQLDISSAYLYADIKEELY 1376
Query: 61 MKQPEGFVIKGQVQKVCKLTKSLYELKQT 89
++ P G K+ +L KSLY LKQ+
Sbjct: 1377 IRPPPHL---GLNDKLLRLRKSLYGLKQS 1402
>YJZ7_YEAST (P47098) Transposon Ty1 protein B
Length = 1755
Score = 39.7 bits (91), Expect = 0.001
Identities = 27/89 (30%), Positives = 45/89 (50%), Gaps = 8/89 (8%)
Query: 6 FTQKEGIGYFDTYAPVARITTIR-----VLLALASLYKFVIHQMDVKTAFLNGELDEEVY 60
F + I + DTY + T+ L+LA + I Q+D+ +A+L ++ EE+Y
Sbjct: 1302 FVARGDIQHPDTYDTGMQSNTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIKEELY 1361
Query: 61 MKQPEGFVIKGQVQKVCKLTKSLYELKQT 89
++ P G K+ +L KS Y LKQ+
Sbjct: 1362 IRPPPHL---GMNDKLIRLKKSHYGLKQS 1387
>M820_ARATH (P92520) Hypothetical mitochondrial protein AtMg00820
(ORF170)
Length = 170
Score = 34.3 bits (77), Expect = 0.052
Identities = 15/29 (51%), Positives = 21/29 (71%)
Query: 6 FTQKEGIGYFDTYAPVARITTIRVLLALA 34
F Q+EGI + +TY+PV R TIR +L +A
Sbjct: 97 FHQEEGIYFVETYSPVVRTATIRTILNVA 125
>GLCE_HUMAN (O94923) D-glucuronyl C5-epimerase (EC 5.1.3.-)
(Heparin/heparan sulfate:glucuronic acid C5 epimerase)
(Fragment)
Length = 609
Score = 33.5 bits (75), Expect = 0.089
Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 4/57 (7%)
Query: 37 YKFVIHQMDVKTAFLNGELDEEVYMKQPEGFVIKGQVQKVCKLTKSLYELKQTPKKK 93
YKF+ Q VK F+N E Y P FV+ G + + LY+LK+T +K
Sbjct: 469 YKFLSEQHGVKAVFMNKHDWYEEYPTTPSSFVLNGFMYSLI----GLYDLKETAGEK 521
>GLCE_BOVIN (O18756) D-glucuronyl C5 epimerase (EC 5.1.3.-)
(Heparin/heparan sulfate:glucuronic acid C5 epimerase)
Length = 617
Score = 33.5 bits (75), Expect = 0.089
Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 4/57 (7%)
Query: 37 YKFVIHQMDVKTAFLNGELDEEVYMKQPEGFVIKGQVQKVCKLTKSLYELKQTPKKK 93
YKF+ Q VK F+N E Y P FV+ G + + LY+LK+T +K
Sbjct: 477 YKFLSEQHGVKAVFMNKHDWYEEYPTTPSSFVLNGFMYSLI----GLYDLKETAGEK 529
>GLCE_MOUSE (Q9EPS3) D-glucuronyl C5-epimerase (EC 5.1.3.-)
(Heparin/heparan sulfate:glucuronic acid C5 epimerase)
Length = 618
Score = 31.2 bits (69), Expect = 0.44
Identities = 19/53 (35%), Positives = 25/53 (46%), Gaps = 4/53 (7%)
Query: 37 YKFVIHQMDVKTAFLNGELDEEVYMKQPEGFVIKGQVQKVCKLTKSLYELKQT 89
YKF Q VK F+N E Y P FV+ G + + LY+LK+T
Sbjct: 478 YKFPSEQHGVKAVFMNKHDWYEEYPTTPSSFVLNGFMYSLI----GLYDLKET 526
>GSA_PYRAE (Q8ZYW1) Probable glutamate-1-semialdehyde
2,1-aminomutase (EC 5.4.3.8) (GSA)
(Glutamate-1-semialdehyde aminotransferase) (GSA-AT)
Length = 423
Score = 29.6 bits (65), Expect = 1.3
Identities = 24/86 (27%), Positives = 41/86 (46%), Gaps = 5/86 (5%)
Query: 29 VLLALASLYKFVIHQMDVKTAFLNGELDEEVYMKQPEGFVIKGQVQKVCKLTKSLYELKQ 88
V+ +AS+++F + DVKT E +E++Y+K E +K V LT S YE+
Sbjct: 340 VVKRMASMFQFYFKKGDVKTPQDVRESNEKMYLKLHE-IALKHGVY----LTPSQYEVNF 394
Query: 89 TPKKKWHQKFDQVVLAHEYKINEFDK 114
T + ++ + A E E +
Sbjct: 395 TSAAHGREVVEETLAALEKSFQELQR 420
>SYE2_RICCN (Q92H06) Glutamyl-tRNA synthetase 2 (EC 6.1.1.17)
(Glutamate--tRNA ligase 2) (GluRS 2)
Length = 513
Score = 28.9 bits (63), Expect = 2.2
Identities = 26/111 (23%), Positives = 48/111 (42%), Gaps = 19/111 (17%)
Query: 9 KEGIGYFDTYAP--VARITTIRVLLALASLYKFVIHQMDVKTAFLNGELDEEVYMKQPEG 66
++ IGY P + R T+ L LA +Y +D + Q
Sbjct: 387 EQEIGYIKQAMPSLLVRSETLLELTRLAQIYL----------------VDSPIIYSQDSK 430
Query: 67 FVIKGQVQKVCK-LTKSLYELKQTPKKKWHQKFDQVVLAHEYKINEFDKCV 116
+I+ + + K + ++L EL+Q K+ KF ++ A++ K+N+ K V
Sbjct: 431 EIIENCDKNLIKQIIENLSELEQFDKESVQNKFKEIAAANDLKLNDIMKPV 481
>PYRG_WIGBR (Q8D2K0) CTP synthase (EC 6.3.4.2) (UTP--ammonia ligase)
(CTP synthetase)
Length = 552
Score = 28.5 bits (62), Expect = 2.9
Identities = 13/53 (24%), Positives = 27/53 (50%)
Query: 31 LALASLYKFVIHQMDVKTAFLNGELDEEVYMKQPEGFVIKGQVQKVCKLTKSL 83
L L +F+ +M K +F +G++ EV + +GF + +Q + +T +
Sbjct: 77 LDLGHYERFIKTKMSKKNSFTSGKIYSEVLKNERKGFYLGSTIQVIPHITNEI 129
>TOP1_BUCAI (P57371) DNA topoisomerase I (EC 5.99.1.2)
(Omega-protein) (Relaxing enzyme) (Untwisting enzyme)
(Swivelase)
Length = 861
Score = 28.1 bits (61), Expect = 3.7
Identities = 22/77 (28%), Positives = 34/77 (43%), Gaps = 10/77 (12%)
Query: 47 KTAFLNGELDEE---VYMKQPEGFVIKGQVQKVCKLTKSLYELKQTPK-------KKWHQ 96
KT FL +L EE YM+ ++ + ++KV K KS Y PK +K Q
Sbjct: 302 KTMFLAQKLYEEGYITYMRTDSNYLSEYAIKKVRKYIKSNYGSNYLPKEPNIYSNEKHSQ 361
Query: 97 KFDQVVLAHEYKINEFD 113
+ + + + KI D
Sbjct: 362 EAHEAIRPSDIKIKNID 378
>GRSB_BACBR (P14688) Gramicidin S synthetase II [Includes:
ATP-dependent proline adenylase (ProA) (Proline
activase); ATP-dependent valine adenylase (ValA) (Valine
activase); ATP-dependent ornithine adenylase (OrnA)
(Ornithine activase); ATP-dependent leu
Length = 4451
Score = 27.7 bits (60), Expect = 4.9
Identities = 18/67 (26%), Positives = 31/67 (45%), Gaps = 5/67 (7%)
Query: 50 FLNGELDEEVYMKQPEGFVIKGQVQKVCKLTKSLYELKQTPKKKWHQKFDQVVLAHEYKI 109
F+ G+LD + + + IK V + L S Y L P ++ HQ + + E K
Sbjct: 2126 FIEGKLDYQRFE-----YAIKSLVNRHEALRTSFYSLNGEPVQRVHQNVELQIAYSEAKE 2180
Query: 110 NEFDKCV 116
+E ++ V
Sbjct: 2181 DEIEQIV 2187
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.328 0.143 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,142,524
Number of Sequences: 164201
Number of extensions: 478955
Number of successful extensions: 1490
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 19
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1460
Number of HSP's gapped (non-prelim): 26
length of query: 116
length of database: 59,974,054
effective HSP length: 92
effective length of query: 24
effective length of database: 44,867,562
effective search space: 1076821488
effective search space used: 1076821488
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0592.7