BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0588b.10
(328 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
K2C5_HUMAN (P13647) Keratin, type II cytoskeletal 5 (Cytokeratin... 37 0.052
MAPA_MOUSE (Q9QYR6) Microtubule-associated protein 1A (MAP 1A) (... 37 0.089
KF3A_HUMAN (Q9Y496) Kinesin-like protein KIF3A (Microtubule plus... 37 0.089
ST31_HUMAN (Q9BXU1) Serine/threonine-protein kinase 31 (EC 2.7.1... 36 0.15
CN3A_MOUSE (Q9Z0X4) cGMP-inhibited 3',5'-cyclic phosphodiesteras... 35 0.34
CN3A_RAT (Q62865) cGMP-inhibited 3',5'-cyclic phosphodiesterase ... 34 0.44
RPOC_CHLCV (Q822J2) DNA-directed RNA polymerase beta' chain (EC ... 33 0.75
RIP1_HUMAN (O95153) Peripheral-type benzodiazepine receptor-asso... 33 0.75
NFL_COTJA (Q02916) Neurofilament triplet L protein (Neurofilamen... 33 0.75
MAPB_RAT (P15205) Microtubule-associated protein 1B (MAP 1B) (Ne... 33 0.75
K22O_HUMAN (Q01546) Keratin, type II cytoskeletal 2 oral (Cytoke... 33 0.75
RPOC_CHLMU (Q9PK79) DNA-directed RNA polymerase beta' chain (EC ... 33 0.98
REST_HUMAN (P30622) Restin (Cytoplasmic linker protein-170 alpha... 33 0.98
K2CF_HUMAN (P48669) Keratin, type II cytoskeletal 6F (Cytokerati... 33 0.98
K2CE_HUMAN (P48668) Keratin, type II cytoskeletal 6E (Cytokerati... 33 0.98
K2CD_HUMAN (P48667) Keratin, type II cytoskeletal 6D (Cytokerati... 33 0.98
K2CC_HUMAN (P48666) Keratin, type II cytoskeletal 6C (Cytokerati... 33 0.98
K2CB_HUMAN (P04259) Keratin, type II cytoskeletal 6B (Cytokerati... 33 0.98
K2CA_HUMAN (P02538) Keratin, type II cytoskeletal 6A (Cytokerati... 33 0.98
Y149_MYCPN (P75583) Hypothetical lipoprotein MG149 homolog precu... 33 1.3
>K2C5_HUMAN (P13647) Keratin, type II cytoskeletal 5 (Cytokeratin 5)
(K5) (CK 5) (58 kDa cytokeratin)
Length = 590
Score = 37.4 bits (85), Expect = 0.052
Identities = 31/131 (23%), Positives = 65/131 (48%), Gaps = 8/131 (6%)
Query: 192 STRQKKSGDGVYATSSD---RSASIKGDEYEATQPATRPLGKKNQKRKAKVGDTASSDLD 248
+ R + + Y T + ++A GD+ T+ + + Q+ +A++ D
Sbjct: 350 ANRSRTEAESWYQTKYEELQQTAGRHGDDLRNTKHEITEMNRMIQRLRAEI-DNVKKQCA 408
Query: 249 YVPNSEMIAIGKAKLGFLASFEKLKTEKLEVKKEKNKLQKARLLKEYKDILMEDTSEMNE 308
+ N+ A + +L + KL +LE +K K ARLL+EY++++ +T +
Sbjct: 409 NLQNAIADAEQRGELALKDARNKLA--ELEEALQKAKQDMARLLREYQELM--NTKLALD 464
Query: 309 VQLATHQRLVE 319
V++AT+++L+E
Sbjct: 465 VEIATYRKLLE 475
>MAPA_MOUSE (Q9QYR6) Microtubule-associated protein 1A (MAP 1A)
(Fragment)
Length = 1021
Score = 36.6 bits (83), Expect = 0.089
Identities = 40/153 (26%), Positives = 70/153 (45%), Gaps = 15/153 (9%)
Query: 177 VKDEPKWKGTFMTTSSTRQKKSGDGVYATSSDRSASIKGDEYEATQPATRPLGKKNQKRK 236
+K PK T M+ + R++ +G S+ + + E +A +P+ +P K ++ +
Sbjct: 547 LKAAPK---TAMSKLAKREEVLEEGAKEARSELAKELAKSEKKAKEPSEKPPEKPSKPER 603
Query: 237 AKVGDTASSDLDYVPNSEMIA--IGKAKLGFLASF--EKLKTEKLEVKKEKNKLQKARLL 292
+ T SS+ ++I +GK L S EK EK E+KKE+ +L+K
Sbjct: 604 VR---TESSEALKAEKRKLIKDKVGKKHLKEKISKLEEKRDKEKKEIKKERKELKKEEGR 660
Query: 293 KEYKDILMEDTSEMNEVQLATHQRLVEFAMKEL 325
KE K +D E + T L +F+ +L
Sbjct: 661 KEEKKDAKKD-----EKRKDTKPELKKFSKPDL 688
>KF3A_HUMAN (Q9Y496) Kinesin-like protein KIF3A (Microtubule plus
end-directed kinesin motor 3A)
Length = 702
Score = 36.6 bits (83), Expect = 0.089
Identities = 43/179 (24%), Positives = 77/179 (42%), Gaps = 18/179 (10%)
Query: 123 NKLNADLQKFVGCHTKAVNHWKSGHSDKDIMATARQLYHVDTGKDFKHENEWRLVKDEPK 182
+KL LQ +G ++K + G +D + T L + + K+ K++ + ++PK
Sbjct: 300 SKLTRLLQDSLGGNSKTMMCANIGPADYNYDETISTLRYANRAKNIKNKAR---INEDPK 356
Query: 183 --WKGTFMTTSSTRQKKSGDGVYATSSDRSASIKGDEYEATQPATRPLGKKNQKRKAKVG 240
F +KK +G + SD S S + D+ E +G+ +KRK +
Sbjct: 357 DALLRQFQKEIEELKKKLEEGEEISGSDISGSEEDDDEEGE------VGEDGEKRKKRRD 410
Query: 241 DTASSDLDYVPNSEMIAIGKAKLGFLASFEKLKTEKLEVKKEKNKLQKARLLKEYKDIL 299
T + EM +AK+ K KL++++E+ +A L K KD+L
Sbjct: 411 QTGKKKVSPDKMIEM----QAKID---EERKALETKLDMEEEERNKARAELEKREKDLL 462
>ST31_HUMAN (Q9BXU1) Serine/threonine-protein kinase 31 (EC
2.7.1.37) (Serine/threonine-protein kinase NYD-SPK)
Length = 1019
Score = 35.8 bits (81), Expect = 0.15
Identities = 26/87 (29%), Positives = 42/87 (47%), Gaps = 1/87 (1%)
Query: 225 TRPLGKKNQKRKAKVGDTASSDLDYVPNSEMIAIGKAKLGFLASFEKLKTE-KLEVKKEK 283
+RP G ++K + D + E +A+G LG S EK+K + KL + EK
Sbjct: 253 SRPKGHLSEKMTLDLKDENDAGNLITFPKESLAVGDFNLGSNVSLEKIKQDQKLIEENEK 312
Query: 284 NKLQKARLLKEYKDILMEDTSEMNEVQ 310
K +K LL+ YK + ++ E+Q
Sbjct: 313 LKTEKDALLESYKALELKVEQIAQELQ 339
>CN3A_MOUSE (Q9Z0X4) cGMP-inhibited 3',5'-cyclic phosphodiesterase A
(EC 3.1.4.17) (Cyclic GMP inhibited phosphodiesterase A)
(CGI-PDE A)
Length = 1141
Score = 34.7 bits (78), Expect = 0.34
Identities = 15/36 (41%), Positives = 22/36 (60%)
Query: 13 PPQYQAPPTGSSSSSKVSSTQCEAMPEEPEFSTQRG 48
P +PP GS +SS VS++ + PE PE + +RG
Sbjct: 523 PSPSSSPPQGSPASSPVSNSASQQFPESPEVTIKRG 558
>CN3A_RAT (Q62865) cGMP-inhibited 3',5'-cyclic phosphodiesterase A
(EC 3.1.4.17) (Cyclic GMP inhibited phosphodiesterase A)
(CGI-PDE A)
Length = 1141
Score = 34.3 bits (77), Expect = 0.44
Identities = 16/36 (44%), Positives = 20/36 (55%)
Query: 13 PPQYQAPPTGSSSSSKVSSTQCEAMPEEPEFSTQRG 48
P +PP GS +SS VS PE PE +T+RG
Sbjct: 523 PSPSSSPPQGSPTSSPVSGIASVQFPESPEVTTKRG 558
>RPOC_CHLCV (Q822J2) DNA-directed RNA polymerase beta' chain (EC
2.7.7.6) (RNAP beta' subunit) (Transcriptase beta'
chain) (RNA polymerase beta' subunit)
Length = 1393
Score = 33.5 bits (75), Expect = 0.75
Identities = 34/131 (25%), Positives = 53/131 (39%), Gaps = 27/131 (20%)
Query: 193 TRQKKSGDGVYATSSDRSASIKGDEYEATQPATRPLGKKNQKRKAKVGDTASSDLDYVPN 252
T + G+ + T+SD I +EY P L KK A+ +
Sbjct: 121 TTPSRIGNVLGMTASDLERIIYYEEYVVIDPGKTDLNKKQLLNDAQYREVVEK------- 173
Query: 253 SEMIAIGK----AKLGFLASFEKLKTEKLEVKKEKNKLQKARLLKEYKDILMEDTSEMNE 308
GK AK+G A ++ LK+E L+ LLKE KD L + S+
Sbjct: 174 -----WGKDAFVAKMGGEAIYDLLKSEDLQ-----------SLLKELKDRLRKTKSQQAR 217
Query: 309 VQLATHQRLVE 319
++LA +++E
Sbjct: 218 MKLAKRLKIIE 228
>RIP1_HUMAN (O95153) Peripheral-type benzodiazepine
receptor-associated protein 1 (PRAX-1) (Peripheral
benzodiazepine receptor interacting protein) (PBR-IP)
(RIM binding protein 1) (RIM-BP1)
Length = 1857
Score = 33.5 bits (75), Expect = 0.75
Identities = 37/139 (26%), Positives = 53/139 (37%), Gaps = 6/139 (4%)
Query: 195 QKKSGDGVYATSSDRSASIKGDEYEATQPATRPLGKKNQKRKAKVGDTASSD-----LDY 249
Q+++G V TS +R+AS + PA L K+ + A ASS
Sbjct: 1153 QEEAGAAVLGTSEERTASTSTLGEKDPGPAAPSLAKQEAEWTAGEACPASSSTQGARAQQ 1212
Query: 250 VPNSEMIAIGKAKLGFLASFEKLKTEKLEVKKEKNKLQKARLLKEYKDILMEDTSEMNEV 309
PN+EM G G EK T +L V + + R + DI E+ E E
Sbjct: 1213 APNTEMCQGGDPGSGLRPRAEKEDTAELGVHLVNSLVDHGR-NSDLSDIQEEEEEEEEEE 1271
Query: 310 QLATHQRLVEFAMKELGMS 328
+ R F + G S
Sbjct: 1272 EEELGSRTCSFQKQVAGNS 1290
>NFL_COTJA (Q02916) Neurofilament triplet L protein (Neurofilament
light polypeptide) (NF-L)
Length = 555
Score = 33.5 bits (75), Expect = 0.75
Identities = 33/148 (22%), Positives = 66/148 (44%), Gaps = 27/148 (18%)
Query: 175 RLVKDEPKWKGTFMTTSSTRQKKSGDGVYATSSDRSAS---IKGDEYEATQPATRPLGKK 231
R +++ +W + T S K+ D V A + S S +K E ATR + +
Sbjct: 272 RNMQNAEEWFRSRFTVLSESAAKNTDAVRAAKDEVSESRRLLKAKTLEIE--ATRGMNEA 329
Query: 232 NQKRKAKVGDTASSDLDYVPNSEMIAIGKAKLGFLASFEKLKTEKLEVKKEKNKLQKARL 291
+K+ ++ + S+D+ + ++ KLE + K + AR
Sbjct: 330 LEKQLQELEEKQSADISALQDT--------------------INKLENELRTTKSEMARY 369
Query: 292 LKEYKDILMEDTSEMNEVQLATHQRLVE 319
LKEY+D+L + ++++A +++L+E
Sbjct: 370 LKEYQDLL--NVKMALDIEIAAYRKLLE 395
>MAPB_RAT (P15205) Microtubule-associated protein 1B (MAP 1B)
(Neuraxin) [Contains: MAP1 light chain LC1]
Length = 2459
Score = 33.5 bits (75), Expect = 0.75
Identities = 31/122 (25%), Positives = 58/122 (47%), Gaps = 11/122 (9%)
Query: 214 KGDEYEATQPATRPLGKKNQKRKAK---VGDTASSDLDYVP---NSEMIAIGKAKLGFLA 267
+ D E+ +PAT+PL K+ ++++K T +S ++ P + E + + K K G +
Sbjct: 537 RADSRESLKPATKPLSSKSVRKESKEEAPEATKASQVEKTPKVESKEKVIVKKDKPGKVE 596
Query: 268 SFEKLKTEKLEVKKEKNKLQKARLLK----EYKDILMEDTSEMNEVQLATHQRLVEFAMK 323
S + TEK KE+ KA + + E K + +D E++ ++ E K
Sbjct: 597 SKPSV-TEKEVPSKEEQSPVKAEVAEKAATESKPKVTKDKVVKKEIKTKPEEKKEEKPKK 655
Query: 324 EL 325
E+
Sbjct: 656 EV 657
>K22O_HUMAN (Q01546) Keratin, type II cytoskeletal 2 oral
(Cytokeratin 2P) (K2P) (CK 2P)
Length = 638
Score = 33.5 bits (75), Expect = 0.75
Identities = 27/129 (20%), Positives = 59/129 (44%), Gaps = 8/129 (6%)
Query: 194 RQKKSGDGVYATSSDR---SASIKGDEYEATQPATRPLGKKNQKRKAKVGDTASSDLDYV 250
R K + +Y T +A GD+ T+ L + Q+ +A++ + + +
Sbjct: 367 RSKSEAEALYQTKLGELQTTAGRHGDDLRNTKSEIMELNRMIQRLRAEIENVKKQNANL- 425
Query: 251 PNSEMIAIGKAKLGFLASFEKLKTEKLEVKKEKNKLQKARLLKEYKDILMEDTSEMNEVQ 310
IA + + K + L+ +K K ARLL++Y++++ + +V+
Sbjct: 426 --QTAIAEAEQRGEMALKDANAKLQDLQTALQKAKDDLARLLRDYQELM--NVKLALDVE 481
Query: 311 LATHQRLVE 319
+AT+++L+E
Sbjct: 482 IATYRKLLE 490
>RPOC_CHLMU (Q9PK79) DNA-directed RNA polymerase beta' chain (EC
2.7.7.6) (RNAP beta' subunit) (Transcriptase beta'
chain) (RNA polymerase beta' subunit)
Length = 1396
Score = 33.1 bits (74), Expect = 0.98
Identities = 36/131 (27%), Positives = 52/131 (39%), Gaps = 27/131 (20%)
Query: 193 TRQKKSGDGVYATSSDRSASIKGDEYEATQPATRPLGKKNQKRKAKVGDTASSDLDYVPN 252
T + G+ + T+SD I +EY P L KK AK +
Sbjct: 121 TTPSRIGNVLGMTASDLERVIYYEEYVVIDPGNTDLVKKQLLNDAKYREVVEK------- 173
Query: 253 SEMIAIGK----AKLGFLASFEKLKTEKLEVKKEKNKLQKARLLKEYKDILMEDTSEMNE 308
GK AK+G A ++ LK+E LE LL E KD L + S+
Sbjct: 174 -----WGKDAFVAKMGGEAVYDLLKSEDLE-----------SLLGELKDRLRKTKSQQAR 217
Query: 309 VQLATHQRLVE 319
++LA ++VE
Sbjct: 218 MKLAKRLKIVE 228
>REST_HUMAN (P30622) Restin (Cytoplasmic linker protein-170 alpha-2)
(CLIP-170) (Reed-Sternberg intermediate filament
associated protein)
Length = 1427
Score = 33.1 bits (74), Expect = 0.98
Identities = 32/116 (27%), Positives = 57/116 (48%), Gaps = 22/116 (18%)
Query: 217 EYEATQPATRPLGKKNQKRKAKVGDTASSDLDYVPNSEMIAIGKAKL------------G 264
E +A AT L K+N+ K+K+ + A+ + NS++IA+ K+KL
Sbjct: 576 EIKALYTATEKLSKENESLKSKL-EHANKE-----NSDVIALWKSKLETAIASHQQAMEE 629
Query: 265 FLASFEK-LKTEKLEVKKEKNKLQKARLLKEYKDILMEDTSEMNEVQLATHQRLVE 319
SF K L TE E + K +++K RL +++ +E+ + + A H + +E
Sbjct: 630 LKVSFSKGLGTETAEFAELKTQIEKMRLDYQHE---IENLQNQQDSERAAHAKEME 682
>K2CF_HUMAN (P48669) Keratin, type II cytoskeletal 6F (Cytokeratin
6F) (CK 6F) (K6f keratin)
Length = 563
Score = 33.1 bits (74), Expect = 0.98
Identities = 19/49 (38%), Positives = 32/49 (64%), Gaps = 2/49 (4%)
Query: 271 KLKTEKLEVKKEKNKLQKARLLKEYKDILMEDTSEMNEVQLATHQRLVE 319
K K E LE +K K ARLLKEY++++ + +V++AT+++L+E
Sbjct: 423 KNKLEGLEDALQKAKQDLARLLKEYQELM--NVKLALDVEIATYRKLLE 469
>K2CE_HUMAN (P48668) Keratin, type II cytoskeletal 6E (Cytokeratin
6E) (CK 6E) (K6e keratin)
Length = 563
Score = 33.1 bits (74), Expect = 0.98
Identities = 19/49 (38%), Positives = 32/49 (64%), Gaps = 2/49 (4%)
Query: 271 KLKTEKLEVKKEKNKLQKARLLKEYKDILMEDTSEMNEVQLATHQRLVE 319
K K E LE +K K ARLLKEY++++ + +V++AT+++L+E
Sbjct: 423 KNKLEGLEDALQKAKQDLARLLKEYQELM--NVKLALDVEIATYRKLLE 469
>K2CD_HUMAN (P48667) Keratin, type II cytoskeletal 6D (Cytokeratin
6D) (CK 6D) (K6d keratin) (Fragment)
Length = 384
Score = 33.1 bits (74), Expect = 0.98
Identities = 19/49 (38%), Positives = 32/49 (64%), Gaps = 2/49 (4%)
Query: 271 KLKTEKLEVKKEKNKLQKARLLKEYKDILMEDTSEMNEVQLATHQRLVE 319
K K E LE +K K ARLLKEY++++ + +V++AT+++L+E
Sbjct: 244 KNKLEGLEDALQKAKQDLARLLKEYQELM--NVKLALDVEIATYRKLLE 290
>K2CC_HUMAN (P48666) Keratin, type II cytoskeletal 6C (Cytokeratin
6C) (CK 6C) (K6c keratin)
Length = 563
Score = 33.1 bits (74), Expect = 0.98
Identities = 19/49 (38%), Positives = 32/49 (64%), Gaps = 2/49 (4%)
Query: 271 KLKTEKLEVKKEKNKLQKARLLKEYKDILMEDTSEMNEVQLATHQRLVE 319
K K E LE +K K ARLLKEY++++ + +V++AT+++L+E
Sbjct: 423 KNKLEGLEDALQKAKQDLARLLKEYQELM--NVKLALDVEIATYRKLLE 469
>K2CB_HUMAN (P04259) Keratin, type II cytoskeletal 6B (Cytokeratin
6B) (CK 6B) (K6b keratin)
Length = 563
Score = 33.1 bits (74), Expect = 0.98
Identities = 19/49 (38%), Positives = 32/49 (64%), Gaps = 2/49 (4%)
Query: 271 KLKTEKLEVKKEKNKLQKARLLKEYKDILMEDTSEMNEVQLATHQRLVE 319
K K E LE +K K ARLLKEY++++ + +V++AT+++L+E
Sbjct: 423 KNKLEGLEDALQKAKQDLARLLKEYQELM--NVKLALDVEIATYRKLLE 469
>K2CA_HUMAN (P02538) Keratin, type II cytoskeletal 6A (Cytokeratin
6A) (CK 6A) (K6a keratin)
Length = 563
Score = 33.1 bits (74), Expect = 0.98
Identities = 19/49 (38%), Positives = 32/49 (64%), Gaps = 2/49 (4%)
Query: 271 KLKTEKLEVKKEKNKLQKARLLKEYKDILMEDTSEMNEVQLATHQRLVE 319
K K E LE +K K ARLLKEY++++ + +V++AT+++L+E
Sbjct: 423 KNKLEGLEDALQKAKQDLARLLKEYQELM--NVKLALDVEIATYRKLLE 469
>Y149_MYCPN (P75583) Hypothetical lipoprotein MG149 homolog
precursor (VXpSPT7_orf320)
Length = 320
Score = 32.7 bits (73), Expect = 1.3
Identities = 26/83 (31%), Positives = 41/83 (49%), Gaps = 8/83 (9%)
Query: 238 KVGDTASSDLDYVPNSEMIAIGKAKLGF---LASFEKLKTEKLEVKKEKNKLQKARLLKE 294
K G+T D D+ E + +G+A L F L LK EK E KKE K+ +
Sbjct: 169 KNGETHEHDHDHHEGEEEVIVGRA-LSFANGLFLVVDLKEEKHEEKKEA----KSEMSMN 223
Query: 295 YKDILMEDTSEMNEVQLATHQRL 317
KD++M +EM ++ + Q++
Sbjct: 224 SKDMVMMTKTEMMSKEMKSEQKM 246
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.310 0.127 0.365
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,395,563
Number of Sequences: 164201
Number of extensions: 1678991
Number of successful extensions: 4701
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 50
Number of HSP's that attempted gapping in prelim test: 4677
Number of HSP's gapped (non-prelim): 70
length of query: 328
length of database: 59,974,054
effective HSP length: 110
effective length of query: 218
effective length of database: 41,911,944
effective search space: 9136803792
effective search space used: 9136803792
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 66 (30.0 bits)
Lotus: description of TM0588b.10